Chemistry & Biodiversity,
Journal Year:
2024,
Volume and Issue:
21(12)
Published: Aug. 22, 2024
Abstract
Structural
modification
is
an
effective
way
to
improve
the
antifungal
activity
of
natural
products
and
has
been
widely
used
in
development
novel
fungicides.
In
this
work,
a
series
aminocoumarin‐based
Schiff
bases
were
synthesized
characterized
by
1
H‐NMR,
13
C
NMR
HR‐MS
spectra.
The
vitro
inhibition
all
compounds
was
tested
against
four
phytopathogenic
fungi
(
Alternaria
solani
,
Fusarium
oxysporum
Botrytis
cinerea
alternata
)
using
mycelial
growth
rate
method.
results
showed
that
most
target
exhibited
significant
activities.
particular,
5b
5c
5d
5h
5n
7c
7n
7p
more
than
commercially
available
fungicides,
chlorothalonil
azoxystrobin.
structure‐activity
relationship
revealed
electron‐withdrawing
groups
with
electronegativity
introduced
at
C‐3
position
improving
inhibitory
halogenated
benzaldehydes
would
be
necessary
preparation
bases.
compound
(EC
50
=8.73
μg/mL)
=26.25
much
better
positive
controls
(chlorothalonil
azoxystrobin).
Therefore,
could
serve
as
promising
lead
for
broad‐spectrum
which
useful
applications
agriculture.
Frontiers in Microbiology,
Journal Year:
2023,
Volume and Issue:
14
Published: Feb. 27, 2023
Bagging-free
apple
is
more
vulnerable
to
postharvest
disease,
which
severely
limits
the
cultivation
pattern
transformation
of
industry
in
China.
This
study
aimed
ascertain
dominant
pathogens
bagging-free
apples,
evaluate
efficacy
essential
oil
(EO)
on
inhibition
fungal
growth,
and
further
clarify
molecular
mechanism
this
action.
By
morphological
characteristics
rDNA
sequence
analyses,
Botryosphaeria
dothidea
(
B.
)
Colletotrichum
gloeosporioides
C.
were
identified
as
main
isolated
from
decayed
apples.
Cinnamon
clove
EO
exhibited
high
inhibitory
activities
against
mycelial
growth
both
vapor
contact
phases
under
vitro
conditions.
at
a
concentration
60
μL
L
−1
significantly
reduced
incidence
lesion
diameter
inoculated
decay
vivo
.
Observations
using
scanning
electron
microscope
(SEM)
transmission
(TEM)
revealed
that
changed
morphology
cellular
ultrastructure
destroyed
integrity
structure
cell
membranes
major
organelles.
Using
RNA
sequencing
bioinformatics,
it
was
demonstrated
treatment
impaired
membrane
biological
function
via
downregulating
genes
involved
component
transmembrane
transport.
Simultaneously,
stronger
binding
affinity
trans
-cinnamaldehyde
eugenol
with
CYP51
assessed
by
silico
analysis,
attenuating
activity
ergosterol
synthesis
enzyme.
Moreover,
pronounced
alternations
oxidation/reduction
reaction
critical
materials
metabolism
EO-treated
also
observed
transcriptomic
data.
Altogether,
these
findings
contributed
novel
antimicrobial
mechanisms
EO,
suggesting
its
potential
use
natural
useful
preservative
for
controlling
spoilage
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(9), P. 4630 - 4638
Published: Feb. 26, 2024
To
investigate
the
potential
application
value
of
dehydroabietic
acid,
27
novel
dehydroabietyl-1,2,4-triazole-5-thioether-based
derivatives
were
designed
and
characterized
by
IR,
Plants,
Journal Year:
2023,
Volume and Issue:
12(4), P. 909 - 909
Published: Feb. 17, 2023
Phytophthora
infestans,
the
notorious
pathogen
of
potato
late
blight,
leads
to
a
severe
decline
in
yields
and
even
harvest
failure.
We
isolated
201
endophytic
isolates
from
healthy
root
tissues
potatoes,
among
which
41
showed
strong
antagonistic
activity
against
P.
infestans.
Further,
tolerance
stress
potential
application
blight
these
were
tested.
Most
them
extremely
tolerant
stresses
such
as
acid-alkali,
temperature,
UV,
salt,
heavy
metal
stress.
However,
some
with
excellent
might
be
pathogenic
potatoes.
Combining
screening
results,
total
14
endophytes
had
comprehensive
performance
all
tests.
In
this
paper,
endophyte
6-5
was
selected
for
preliminary
exploration
anti-oomycete
mechanism.
Analysis
16S
rDNA
sequence
revealed
that
high
homology
corresponding
Bacillus
velezensis
(99.72%)
NCBI
database.
Endophyte
significantly
inhibited
mycelial
growth
an
inhibition
rate
over
90%
vitro
assays,
deformed
hyphal
phenotype
addition,
could
secrete
protease
cellulase,
produce
substances
thermal
stability,
helpful
its
Furthermore,
it
demonstrated
ability
improve
resistance
tubers
blight.
short,
our
study
described
process
isolating
infestans
roots,
further
explored
biocontrol
candidate
strain
control.
Antonie van Leeuwenhoek,
Journal Year:
2024,
Volume and Issue:
117(1)
Published: Feb. 9, 2024
Plant
probiotics
are
live
microbial
cells
or
cultures
that
support
plant
growth
and
control
pathogens
through
different
mechanisms.
They
have
various
effects
on
plants,
including
promotion
the
production
of
indole
acetic
acid
(IAA),
biological
activity
(BCA),
cellulase
enzymes,
thus
inducing
systemic
resistance
increasing
availability
mineral
elements.
The
present
work
aimed
to
study
potential
Achromobacter
marplatensis
Bacillus
velezensis
as
for
field
cultivation
potatoes.
In
vitro
studies
demonstrated
ability
selected
produce
IAA
cellulase,
well
antimicrobial
against
two
infect
Solanum
tuberosum
Fusarium
oxysporum
Ralstonia
solanacearum
under
conditions
at
a
broad
range
temperatures
pH
values.
vivo
A.
B.
S.
plants
grown
in
sandy
clay
loamy
soil
was
detected
after
90
days.
Probiotic
isolates
were
able
tolerate
ultraviolet
radiation
(UV)
exposure
up
hours,
dose
response
curve
exhibited
D
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(4), P. 1573 - 1573
Published: Feb. 13, 2025
Potato
Fusarium
dry
rot
and
wilt
are
the
most
important
soil-
seed-borne
diseases
in
potatoes.
They
cause
high
economic
losses
during
potato
growth
storage
across
world.
Previous
observations
have
shown
that
dryocrassin
ABBA
can
induce
resistance
However,
little
is
known
about
whether
suppress
oxysporum.
In
this
research,
we
determined
exogenous
significantly
inhibited
mycelial
growth,
changed
cell
ultrastructure,
increased
MDA
content,
decreased
antioxidant
enzyme
activity
of
F.
The
transcriptome
analysis
oxysporum
with
or
without
indicated
1244
differentially
expressed
genes
(DEGs)
were
identified,
which
594
upregulated
650
downregulated.
GO
term
showed
DEGs
mostly
related
to
biological
processes.
KEGG
pathway
was
mainly
carbohydrate,
amino
acid,
lipid
metabolism.
Moreover,
expressions
PCWDEs,
HSPs,
MFS
downregulated,
decreasing
stress
capacity
weakening
pathogenicity
treatment.
These
findings
contribute
a
new
understanding
direct
functions
on
provide
potential
ecofriendly
biocontrol
approach
for
wilt.
Plant
diseases
caused
by
vegetable
viruses
are
an
important
threat
to
global
food
security,
presenting
a
major
challenge
for
the
development
of
antiviral
agrochemicals.
Functional
proteins
plant
play
crucial
role
in
viral
life
cycle,
and
targeted
inhibition
these
has
emerged
as
promising
strategy.
However,
current
discovery
small
molecules
is
hampered
limitations
synthetic
approaches
narrow
range
targets.
Herein,
we
report
practical
application
organocatalysis
serving
pesticide
that
bears
unique
molecular
basis.
An
N
-heterocyclic
carbene-modulated
reaction
first
designed
asymmetrically
functionalize
diverse
natural
phenols
with
phthalides.
Our
method
capable
producing
series
new
phthalidyl
ethers
under
mild
conditions
good
yields,
enantioselectivity,
functional
group
tolerance.
Among
these,
compound
(
R
)-
3w
exhibits
excellent
enantioselectivity-preferred
curative
activity
against
potato
virus
Y
(PVY).
Mechanistically,
it
proposed
interacts
nuclear
inclusion
body
A
(Nia)
protein
PVY
at
His150
residue.
This
binding
impairs
Nia’s
function
cleavage
polyprotein,
thereby
inhibiting
formation
replication
complex.
The
study
provides
insights
into
advancing
protocol
facilitate
agrochemical
discovery,
our
identified
may
serve
potential
lead
future
research
PVY-Nia
inhibitors.
