Genetic Resources and Crop Evolution, Journal Year: 2023, Volume and Issue: 71(1), P. 83 - 91
Published: Sept. 15, 2023
Language: Английский
BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)
Published: June 15, 2024
Abstract Background Three Amino acid Loop Extension (TALE) belongs to the homeobox group of genes that are important constituents plant systems. The TALE gene family is instrumental not only in growth and development but also plays an essential role regulating response environmental adversaries. Results In present study, we isolated 21 CsTALE from cucumber ( Cucumis sativus L.) genome database. Bioinformatics tools were put place understand structural functional components family. evolutionary analysis dissected them into seven subclades (KNOX-I, KNOX-II, BELL-I BELL-V). c - acting elements promoter region disclosed they key regulators hormonal stress-related processes. Additionally, STRING database advocated concerting proteins with other transcription factors potent developmental biology. CsmiR319 CsmiR167a-3p targeting CsTALE15 CsTALE16 , respectively, further assert importance posttranscriptional-related Tissue-specific expression unfolded fundamental involvement as expressed throughout stages. Under waterlogging stress, CsTALE17 significantly higher values WL, WL-NAA, WL-ETH WL-MeJA-treated samples. Conclusions study reveals evolution functions cucumber. Our work will provide a platform help future researchers address issue stress Yangtze River Delta.
Language: Английский
Citations
7
Functional Plant Biology, Journal Year: 2024, Volume and Issue: 51(4)
Published: April 3, 2024
DNA binding proteins with one finger (Dof) transcription factors are essential for seed development and defence against various biotic abiotic stresses in plants. Genomic analysis of Dof has not been determined yet pitaya (Selenicereus undatus). In this study, we have identified 26 gene family members, renamed as HuDof-1 to HuDof-26, clustered them into seven subfamilies based on conserved motifs, domains, phylogenetic analysis. The pairs members were duplicated by segmental duplications that faced purifying selection, indicated the Ka/Ks ratio values. Promoter regions HuDof genes contain many cis-acting elements related phytohormones including abscisic acid, jasmonic gibberellin, temperature, light. We exposed plants different environmental examined melatonin’s influence expression levels. Signifcant HuDof-2 HuDof-6 observed developmental stages flower buds, flowers, pericarp, pulp. Pitaya subjected stresses, transcriptome was carried out identify role members. RNA-sequencing data reverse quantitative PCR-based revealed three putative candidate (HuDof-1, HuDof-2, HuDof-8), which might diverse roles stresses. Our study provides a theoretical foundation functional through traditional modern biotechnological tools trait improvement.
Language: Английский
Citations
6
Plants, Journal Year: 2024, Volume and Issue: 13(7), P. 942 - 942
Published: March 25, 2024
Brassica napus (B. napus) is susceptible to multiple abiotic stresses that can affect plant growth and development, ultimately reducing crop yields. In the past, many genes provide tolerance have been identified characterized. Peroxidase (POD) proteins, members of oxidoreductase enzyme family, play a critical role in protecting plants against stresses. This study demonstrated comprehensive investigation POD gene family B. napus. As result, total 109 were across 19 chromosomes classified into five distinct subgroups. Further, 44 duplicate events identified; these, two pairs tandem 42 segmental. Synteny analysis revealed segmental duplication was more prominent than among genes. Expression pattern based on RNA-seq data indicated BnPOD expressed differently various tissues; most them roots rather other tissues. To validate these findings, we performed RT-qPCR ten genes; showed expression levels under Our findings not only furnish valuable insights evolutionary dynamics but also serve as foundation for subsequent investigations functional roles
Language: Английский
Citations
4
Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(4)
Published: March 24, 2025
Language: Английский
Citations
0
Plant Stress, Journal Year: 2024, Volume and Issue: 12, P. 100492 - 100492
Published: May 16, 2024
Dragon fruit (pitaya) is an important tropical crop with medicinal and industrial values. However, severe environmental stress factors affect dragon production development. The MADS-box gene family performs essential role in the regulation of tolerance, reproduction, ripening various plants, but relevant studies havn't been conducted on fruit. In this study, identification expression analysis was response to abiotic pitaya seedlings. We identified 67 genes distributed across 11 chromosomes genome. All were named from HuMADS-1 HuMADS-67. phylogenetic tree HuMADS-box constructed together soybean, wheat, Arabidopsis. Based analysis, tropology motif's structure, all divided into 3 groups as Mα, Mδ, MIKC. HuMADS showed a conserved intron-exon structure motif domain organization. patterns varied 15 tissue parts pitaya. subcellular localization proteins predicted that 55.8% total members localized nucleus. syntenic 28 collinearity pairs, which 4 pairs tandem duplication 24 segmental chromosomes. Cis-acting element their regulatory roles plant defense processes. RNA-seq seedlings under single combined stresses drought nickel exhibited higher genes. addition, several enhanced melatonin treatment stresses, thereby enhancing seedling growth To validate RNA-Seq data, RT-qPCR performed 12 genes, revealing six them (HuMADS-14/15/22/62/63/67) play tolerance. Overall, our findings offer key insights for future genetic engineering strategies develop stress-tolerant genotypes.
Language: Английский
Citations
3
BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)
Published: May 31, 2024
Abstract Heavy-metal ATPases (HMAs) play a vital role in plants, helping to transport heavy metal ions across cell membranes.However, insufficient data exists concerning HMAs genes within the Arecaceae family.In this study, 12 AcHMA were identified genome of Areca catechu , grouped into two main clusters based on their phylogenetic relationships.Genomic distribution analysis reveals that unevenly distributed six chromosomes. We further analyzed physicochemical properties, collinearity, and gene structure.Furthermore, RNA-seq exhibited varied expressions different tissues A. found AcHMA1 AcHMA2 AcHMA7 highly expressed roots, leaves, pericarp, male/female flowers. A total candidate selected expression patterns, roots leaves was determined using RT-qPCR under stress. Results showed levels AcHMA3 significantly up-regulated Cd2 + Zn2 Similarly, response Cu 2+, AcHMA5 AcHMA8 revealed highest respectively. In conclusion, study will offer foundation for exploring family dealing with stress conditions .
Language: Английский
Citations
3
Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(4)
Published: July 1, 2024
Abstract Under changing climatic conditions, plants are simultaneously facing conflicting stresses in nature. Plants can sense different stresses, induce systematic ROS signals, and regulate transcriptomic, hormonal, stomatal responses. We performed transcriptome analysis to reveal the integrative stress response regulatory mechanism underlying heavy metal alone or combination with heat drought conditions pitaya (dragon fruit). A total of 70 genes were identified from 31,130 transcripts conserved differential expression. Furthermore, weighted gene co‐expression network (WGCNA) trait‐associated modules. By integrating information three modules protein–protein interaction (PPI) networks, we 10 interconnected associated multifaceted defense employed by against co‐occurring stresses. To further confirm reliability results, a comparative 350 trait exhibiting their dynamic expression under all treatments. Differential pattern analysis, have proven instrumental identifying ten putative structural genes. These annotated as PLAT/LH2 , CAT MLP HSP PB1 PLA NAC HMA CER1 transcription factors involved antioxidant activity, response, MAPK signaling, detoxification metals regulating crosstalk between complex pathways. Predictive candidate genes, potentially governing single, double, multifactorial several signaling systems molecular patterns. findings represent valuable resource for breeding programs, offering potential develop resilient “super pitaya” plants.
Language: Английский
Citations
3
Plant Stress, Journal Year: 2023, Volume and Issue: 11, P. 100300 - 100300
Published: Nov. 22, 2023
Multidrug and toxic compound extrusion (MATE) are transporter proteins exists widely in all living organisms which involved toxins detoxification. In plants, MATE functions detoxification of endogenous secondary metabolites, exogenous agents, other plant developmental processes. this study, the identification expression analysis gene family was conducted to analyse response against heavy metal salt stresses pitaya seedlings. We have identified analysed 35 from genome were mapped on 11 chromosomes. All members named HuMATE-1 HuMATE-35, divided into 14 groups based phylogenetic analysis, tree topology motif's structure. The subcellular localization predicted data showed that 62% total localized plasma membrane. syntenic collinearity pairs two tandem duplication twelve segmental Genes motif composition exon-intron structures found more similar within same group. Cis-acting element promoter regions their regulatory function toxin defence RNA-Seq HuMATE candidate genes exhibits higher under copper individually as well both combination. Melatonin applications regulate effectively for stresses, thus enhancing seedling growth development. Moreover, RT-qPCR highly expressed 10 at different stages validates results. Our finding predicts (HuMATE-1/7/8/10/11/12/13/16/28/35) may play a key role produced by metals accumulation high soil salinity. Furthermore, our results provide foundation development stress-tolerant genotypes various climate scenarios through forward reverse genetic breeding programs.
Language: Английский
Citations
6
South African Journal of Botany, Journal Year: 2023, Volume and Issue: 163, P. 145 - 156
Published: Oct. 27, 2023
Language: Английский
Citations
5
Plant Stress, Journal Year: 2023, Volume and Issue: 10, P. 100294 - 100294
Published: Nov. 16, 2023
The SQUAMOSA PROMOTER BINDING PROTEIN (SBP) gene family is essential for plant growth and development. However, little known about the SBP in pitaya (Selenicereus undatus L.); a fruit crop cultivated tropical climates, severely affected by abiotic stress factors. These stresses produce excessive reactive oxygen species (ROS), affecting In this study, we identified 17 genes genome (HuSBP) classified them into seven distinct clusters based on their phylogeny. HuSBP exhibited conserved intron-exon structure motif domain organization. were differentially expressed 15 developmental stages of flower (F), buds (FB), pericarp (PeriC), pulp. addition, specific higher expression under single-factor, double-factor, multifactorial stresses, revealing diverse role development pitaya. Exogenous application melatonin with antioxidant stress-protective properties, was also applied. pattern RNA-Seq data validated RT-qPCR analysis. Based genome-wide results transcriptome-based predicted five putative candidate (HuSBP1/3/7/11/14) that might play roles contain vital enzymes production phenylpropanoid glutathione metabolism. provide valuable insights tolerance could be used to develop innovative strategies create resilient adaptable genotypes using conventional breeding modern biotechnological tools.
Language: Английский
Citations
5