The Science of The Total Environment,
Journal Year:
2021,
Volume and Issue:
784, P. 147183 - 147183
Published: April 19, 2021
This
study
is
the
first
focused
on
presence
of
SARS-CoV-2
in
different
freshwater
environments
an
urban
setting.
Groundwater
and
surface
water
reservoirs
for
drinking
as
well
from
receiving
rivers
Monterrey
Metropolitan
Area
were
sampled
repeatedly
during
a
peak
phase
between
October
2020
January
2021,
viral
RNA
was
measured
by
quantitative
reverse
transcription
polymerase
chain
reaction.
Forty-four
percent
groundwater
samples
had
detectable
loads
2.6
38.3
copies/ml.
A
significant
correlation
load
sucralose
concentration
reaffirmed
hypothesis
leaching
infiltrating
effluent
and/or
failing
sewage
pipes
emphasized
importance
disinfection.
Twelve
dam
tested
positive
RNA,
with
values
varying
3.3
3.8
Finally,
13%
river
concentrations
ranging
2.5
to
7.0
Untreated
wastewater
taken
same
period
showed
up
3535
copies/ml,
demonstrating
dilution
effect
facilities
efficiency
three
orders
magnitude.
Variations
over
time
at
submetropolitan
level
generally
reflected
reported
trends
infection
cases
Monterrey.
The
represent
low
risk
recreational
activities
according
preliminary
assessment
model.
However,
this
result
should
not
be
lightly
due
uncertainty
regarding
data
model
constraints
possibility
situations
where
may
increase
considerably.
ACS ES&T Water,
Journal Year:
2022,
Volume and Issue:
2(11), P. 1992 - 2003
Published: May 26, 2022
Severe
acute
respiratory
syndrome
coronavirus-2
(SARS-CoV-2)
in
wastewater
has
been
used
to
track
community
infections
of
coronavirus
disease-2019
(COVID-19),
providing
critical
information
for
public
health
interventions.
Since
levels
are
dependent
upon
human
inputs,
we
hypothesize
that
tracking
can
be
improved
by
normalizing
concentrations
against
indicators
waste
[Pepper
Mild
Mottle
Virus
(PMMoV),
β-2
Microglobulin
(B2M),
and
fecal
coliform].
In
this
study,
analyzed
SARS-CoV-2
from
two
sewersheds
different
scales:
a
University
campus
treatment
plant.
Wastewater
data
were
combined
with
complementary
COVID-19
case
evaluate
the
efficiency
surveillance
forecasting
new
cases
and,
larger
scale,
hospitalizations.
Results
show
normalization
PMMoV
B2M
resulted
correlations
using
volcano
second
generation
(V2G)-qPCR
chemistry
(rs
=
0.69
without
normalization,
rs
0.73
normalization).
Mixed
results
obtained
samples
collected
at
scale.
Overall
benefits
measures
depend
qPCR
improves
smaller
sewershed
We
recommend
further
studies
efficacy
additional
targets.
The Science of The Total Environment,
Journal Year:
2022,
Volume and Issue:
821, P. 153291 - 153291
Published: Jan. 25, 2022
As
COVID-19
continues
to
spread
globally,
monitoring
the
disease
at
different
scales
is
critical
support
public
health
decision
making.
Surveillance
for
SARS-CoV-2
RNA
in
wastewater
can
supplement
surveillance
based
on
diagnostic
testing.
In
this
paper,
we
report
results
of
wastewater-based
Emory
University
campus
that
included
routine
sampling
sewage
from
a
hospital
building,
an
isolation/quarantine
and
21
student
residence
halls
between
July
13th,
2020
March
14th,
2021.
We
examined
sensitivity
detecting
cases
building
level
relation
Ct
values
RT-qPCR
samples
number
patients
residing
building.
Our
show
weekly
using
Moore
swab
was
not
sensitive
enough
(6
63
times)
reliably
detect
one
or
two
sporadic
The
over
time
same
location
reflected
temporal
trend
(Pearson's
r
<
−0.8),
but
there
too
much
uncertainty
directly
estimate
values.
After
students
returned
spring
2021
semester,
detected
most
hall
sites
weeks
before
surged
campus.
This
finding
suggests
be
used
provide
early
warning
outbreaks
institutions.
The Science of The Total Environment,
Journal Year:
2023,
Volume and Issue:
892, P. 164495 - 164495
Published: May 26, 2023
Wastewater-based
surveillance
can
be
a
valuable
tool
to
monitor
viral
circulation
and
serve
as
an
early
warning
system.
For
respiratory
viruses
that
share
similar
clinical
symptoms,
namely
SARS-CoV-2,
influenza,
syncytial
virus
(RSV),
identification
in
wastewater
may
allow
differentiation
between
seasonal
outbreaks
COVID-19
peaks.
In
this
study,
these
well
standard
indicators
of
fecal
contamination,
weekly
sampling
campaign
was
carried
out
for
15
months
(from
September
2021
November
2022)
two
treatment
plants
the
entire
population
Barcelona
(Spain).
Samples
were
concentrated
by
aluminum
hydroxide
adsorption-precipitation
method
then
analyzed
RNA
extraction
RT-qPCR.
All
samples
positive
while
positivity
rates
influenza
RSV
significantly
lower
(10.65
%
A
(IAV),
0.82
B
(IBV),
37.70
RSV-A
34.43
RSV-B).
Gene
copy
concentrations
SARS-CoV-2
often
approximately
1
2
logarithmic
units
higher
compared
other
viruses.
Clear
peaks
IAV
H3:N2
February
March
2022
winter
observed,
which
matched
chronological
incidence
infections
recorded
Catalan
Government
database.
conclusion,
data
obtained
from
provided
new
information
on
abundance
area
correlated
favorably
with
data.
Clinical Microbiology Reviews,
Journal Year:
2023,
Volume and Issue:
37(1)
Published: Dec. 14, 2023
SUMMARY
Wastewater-based
surveillance
(WBS)
has
undergone
dramatic
advancement
in
the
context
of
coronavirus
disease
2019
(COVID-19)
pandemic.
The
power
and
potential
this
platform
technology
were
rapidly
realized
when
it
became
evident
that
not
only
did
WBS-measured
SARS-CoV-2
RNA
correlate
strongly
with
COVID-19
clinical
within
monitored
populations
but
also,
fact,
functioned
as
a
leading
indicator.
Teams
from
across
globe
innovated
novel
approaches
by
which
wastewater
could
be
collected
diverse
sewersheds
ranging
treatment
plants
(enabling
community-level
surveillance)
to
more
granular
locations
including
individual
neighborhoods
high-risk
buildings
such
long-term
care
facilities
(LTCF).
Efficient
processes
enabled
extraction
concentration
highly
dilute
matrix.
Molecular
genomic
tools
identify,
quantify,
characterize
its
various
variants
adapted
programs
applied
these
mixed
environmental
systems.
Novel
data-sharing
allowed
information
mobilized
made
immediately
available
public
health
government
decision-makers
even
public,
enabling
evidence-informed
decision-making
based
on
local
dynamics.
WBS
since
been
recognized
tool
transformative
potential,
providing
near-real-time
cost-effective,
objective,
comprehensive,
inclusive
data
changing
prevalence
measured
analytes
space
time
populations.
However,
consequence
rapid
innovation
hundreds
teams
simultaneously,
tremendous
heterogeneity
currently
exists
literature.
This
manuscript
provides
state-of-the-art
review
established
details
current
work
underway
expanding
scope
other
infectious
targets.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2023,
Volume and Issue:
12
Published: Jan. 5, 2023
The
coronavirus
disease
2019
(COVID-19)
pandemic
has
prompted
a
lot
of
questions
globally
regarding
the
range
information
about
virus’s
possible
routes
transmission,
diagnostics,
and
therapeutic
tools.
Worldwide
studies
have
pointed
out
importance
monitoring
early
surveillance
techniques
based
on
identification
viral
RNA
in
wastewater.
These
indicated
presence
severe
acute
respiratory
syndrome
2
(SARS-CoV-2)
human
feces,
which
is
shed
via
excreta
including
mucus,
saliva,
sputum.
Subsequently,
they
get
dumped
into
wastewater,
their
wastewater
provides
possibility
using
it
as
tool
to
help
prevent
eradicate
virus.
Its
still
done
many
regions
worldwide
serves
an
“warning
signal”;
however,
limitations
also
been
identified.
Environmental Health Perspectives,
Journal Year:
2021,
Volume and Issue:
129(4)
Published: April 1, 2021
Wastewater
testing
offers
a
cost-effective
strategy
for
measuring
population
disease
prevalence
and
health
behaviors.
For
COVID-19,
wastewater
surveillance
addresses
gaps
provides
an
early
warning
outbreaks.
As
U.S.
federal
agencies
build
National
Surveillance
System
around
the
pandemic,
thinking
through
ways
to
develop
flexible
frameworks
sampling,
testing,
reporting
can
avoid
unnecessary
system
overhauls
future
infectious
disease,
chronic
drug
epidemics.
Microbiology Spectrum,
Journal Year:
2021,
Volume and Issue:
9(2)
Published: Oct. 7, 2021
A
wastewater
surveillance
program
targeting
a
university
residence
hall
was
implemented
during
the
spring
semester
2021
as
proactive
measure
to
avoid
an
outbreak
of
COVID-19
on
campus.
Over
period
7
weeks
from
early
February
through
late
March
2021,
originating
collected
grab
samples
3
times
per
week.
During
this
time,
there
no
detection
SARS-CoV-2
by
reverse
transcriptase
quantitative
PCR
(RT-qPCR)
in
stream.
Aiming
obtain
sample
more
representative
community,
decision
made
use
passive
samplers
beginning
onwards.
Adopting
Moore
swab
approach,
detected
just
2
days
after
were
deployed.
These
also
tested
positive
for
B.1.1.7
(Alpha)
variant
concern
(VOC)
using
RT-qPCR.
The
result
triggered
public
health
case-finding
response,
including
mobile
testing
unit
deployed
following
day,
with
nearly
200
students
and
staff,
which
identified
two
laboratory-confirmed
cases
Alpha
COVID-19.
individuals
relocated
separate
quarantine
facility,
averting
Aggregating
clinical
data,
campus
has
yielded
first
estimates
fecal
shedding
rates
VOC
nonclinical
setting.
IMPORTANCE
Among
adopters
monitoring
have
been
colleges
universities
throughout
North
America,
many
whom
are
approach
monitor
congregate
living
facilities
evidence
infection
integral
component
screening
programs.
Yet,
while
numerous
examples
where
among
community
members,
few
response
that
may
averted
actual
outbreak.
This
report
details
wastewater-testing
when
mounting
globally
over
emergence
variants
concern,
reported
be
transmissible
than
wild-type
Wuhan
strain.
In
communication,
we
present
clear
example
how
resulted
actionable
responses
administration
health,
Food and Environmental Virology,
Journal Year:
2021,
Volume and Issue:
14(4), P. 315 - 354
Published: Nov. 2, 2021
The
outbreak
of
coronavirus
infectious
disease-2019
(COVID-19),
caused
by
the
Severe
Acute
Respiratory
Syndrome
Coronavirus
2
(SARS-CoV-2),
has
rapidly
spread
throughout
world.
Several
studies
have
shown
that
detecting
SARS-CoV-2
in
untreated
wastewater
can
be
a
useful
tool
to
identify
new
outbreaks,
establish
trends,
and
assess
prevalence
infections.
On
06
May
2021,
over
year
into
pandemic,
we
conducted
scoping
review
aiming
summarize
research
data
on
sewage.
Papers
dealing
with
raw
sewage
collected
at
treatment
plants,
sewer
networks,
septic
tanks,
sludge
facilities
were
included
this
review.
We
also
reviewed
community
settings
such
as
private
or
municipal
hospitals,
healthcare
facilities,
nursing
homes,
dormitories,
campuses,
airports,
aircraft,
cruise
ships.
literature
search
was
using
electronic
databases
PubMed,
EMBASE,
Web
Science
Core
Collection.
This
comprehensive
yielded
1090
results,
66
which
met
inclusion
criteria
are
discussed
Studies
from
26
countries
worldwide
investigated
occurrence
different
origin.
percentage
positive
samples
ranged
11.6
100%,
viral
concentrations
ranging
˂LOD
4.6
×
108
genome
copies/L.
outlines
evidence
currently
available
surveillance:
(i)
an
early
warning
system
capable
predicting
COVID-19
outbreaks
days
weeks
before
clinical
cases;
(ii)
establishing
trends
current
outbreaks;
(iii)
estimating
infections;
(iv)
studying
genetic
diversity.
In
conclusion,
cost-effective,
rapid,
reliable
source
information
its
variants
population,
surveillance
enhance
genomic
epidemiological
independent
complementary
inform
public
health
decision-making
during
ongoing
pandemic.