Gut-on-Chip microphysiological systems: Latest advances in the integration of sensing strategies and adoption of mature detection mechanisms DOI Creative Commons
M.A. Signore, Chiara De Pascali, Lucia Giampetruzzi

et al.

Sensing and Bio-Sensing Research, Journal Year: 2021, Volume and Issue: 33, P. 100443 - 100443

Published: July 21, 2021

Synergic efforts in microfabrication processes, cells culture and tissue engineering promoted extraordinary progress Organ-on-Chip (OoC) technology, leading to the development of vitro microphysiological models able recapitulate microenvironment key biochemical, functional, structural mechanical features specific tissues living organs. In order assess functionality these cell cultures with every increasing biological complexity, it is also important equip OoCs miniaturized sensing devices monitor physical chemical parameters related pathophysiological cell-cell interactions. Gut one most interesting studied human organs: performs multiple fundamental body functions, from transport, absorption metabolism nutrients drugs, maturation immune system host protection pathogens infections. this Review, an overview Gut-on-Chip (GoC) systems provided, a special attention focused on relevant strategies integrated into GoC, aimed at monitoring situ intestine functionalities. Advantages limitations associated currently physical, chemical, biochemical sensors are discussed, together challenges that technology still faces, possible adaptive solutions coming other developed OoC models. Finally, we focus how gut microbiota connect organs role understanding progression many diseases, such as recent pandemic infection caused by SARS-CoV-2 virus.

Language: Английский

Organs-on-a-chip models for biological research DOI Creative Commons
Gordana Vunjak‐Novakovic, Kacey Ronaldson-Bouchard, Milica Radisic

et al.

Cell, Journal Year: 2021, Volume and Issue: 184(18), P. 4597 - 4611

Published: Sept. 1, 2021

We explore the utility of bioengineered human tissues—individually or connected into physiological units—for biological research. While much smaller and simpler than their native counterparts, these tissues are complex enough to approximate distinct tissue phenotypes: molecular, structural, functional. Unlike organoids, which form spontaneously recapitulate development, "organs-on-a-chip" engineered display some specific functions whole organs. Looking back, we discuss key developments this emerging technology. Thinking forward, focus on challenges faced fully establish, validate, utilize fidelity models for

Language: Английский

Citations

169
COVID-19 Point-of-Care Diagnostics: Present and Future DOI Creative Commons
Enrique Valera, Aaron Jankelow, Jongwon Lim

et al.

ACS Nano, Journal Year: 2021, Volume and Issue: 15(5), P. 7899 - 7906

Published: May 13, 2021

Point-of-care (POC) detection technologies that enable decentralized, rapid, sensitive, low-cost diagnostics of COVID-19 infection are urgently needed around the world. With many approved for commercialization in past 10 months, field POC is rapidly evolving. In this Perspective, we analyze current state diagnosis and monitoring discuss future challenges diagnostics. As pandemic becomes endemic, advances gained during year will likely also be utilized prediction emerging outbreaks pandemics.

Language: Английский

Citations

149
Application of 3D bioprinting in the prevention and the therapy for human diseases DOI Creative Commons
Hee‐Gyeong Yi, Hyeonji Kim, Jun‐Young Kwon

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)

Published: May 14, 2021

Abstract Rapid development of vaccines and therapeutics is necessary to tackle the emergence new pathogens infectious diseases. To speed up drug discovery process, conventional pipeline can be retooled by introducing advanced in vitro models as alternatives disease employing technology for production medicine cell/drug delivery systems. In this regard, layer-by-layer construction with a 3D bioprinting system or other technologies provides beneficial method developing highly biomimetic reliable research. addition, high flexibility versatility offer advantages effective vaccines, therapeutics, relevant Herein, we discuss potential control We also suggest that research could significant platform rapid automated tissue/organ medicines near future.

Language: Английский

Citations

111
Brain-on-a-chip: Recent advances in design and techniques for microfluidic models of the brain in health and disease DOI Creative Commons

Leyla Amirifar,

Amir Shamloo,

Rohollah Nasiri

et al.

Biomaterials, Journal Year: 2022, Volume and Issue: 285, P. 121531 - 121531

Published: April 21, 2022

Recent advances in biomaterials, microfabrication, microfluidics, and cell biology have led to the development of organ-on-a-chip devices that can reproduce key functions various organs. Such platforms promise provide novel insights into physiological events, including mechanisms disease, evaluate effects external interventions, such as drug administration. The neuroscience field is expected benefit greatly from these innovative tools. Conventional ex vivo studies nervous system been limited by inability culture adequately mimic physiology. While animal models be used, their relevance human physiology uncertain use laborious associated with ethical issues. To date, systems developed model different tissue components brain, brain regions specific blood barrier, both normal pathophysiological conditions. still its infancy, it major impact on neurophysiology, pathology neuropharmacology future. Here, we review made limitations faced an effort stimulate next generation brain-on-a-chip devices.

Language: Английский

Citations

97
Organ-on-a-chip meets artificial intelligence in drug evaluation DOI Creative Commons
Shiwen Deng, Caifeng Li, Junxian Cao

et al.

Theranostics, Journal Year: 2023, Volume and Issue: 13(13), P. 4526 - 4558

Published: Jan. 1, 2023

Drug evaluation has always been an important area of research in the pharmaceutical industry. However, animal welfare protection and other shortcomings traditional drug development models pose obstacles challenges to evaluation. Organ-on-a-chip (OoC) technology, which simulates human organs on a chip physiological environment functionality, with high fidelity reproduction organ-level physiology or pathophysiology, exhibits great promise for innovating pipeline. Meanwhile, advancement artificial intelligence (AI) provides more improvements design data processing OoCs. Here, we review current progress that made generate OoC platforms, how single multi-OoCs have used applications, including testing, disease modeling, personalized medicine. Moreover, discuss issues facing field, such as large reproducibility, point integration OoCs AI analysis automation, is benefit future Finally, look forward opportunities faced by coupling AI. In summary, advancements development, combinations AI, will eventually break state

Language: Английский

Citations

56
A microfluidics platform for simultaneous evaluation of sensitivity and side effects of anti-cancer drugs using a three-dimensional culture method DOI Creative Commons
Yuki Kobayashi, Hideo Hashizume,

Sotaro Takiguchi

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 2, 2025

Language: Английский

Citations

2
Innovative Human Three-Dimensional Tissue-Engineered Models as an Alternative to Animal Testing DOI Creative Commons

Patrick Bédard,

Sara Gauvin,

Karel Ferland

et al.

Bioengineering, Journal Year: 2020, Volume and Issue: 7(3), P. 115 - 115

Published: Sept. 17, 2020

Animal testing has long been used in science to study complex biological phenomena that cannot be investigated using two-dimensional cell cultures plastic dishes. With time, it appeared more differences could exist between animal models and even when translated human patients. Innovative became essential develop accurate knowledge. Tissue engineering provides some of those models, but mostly relies on the use prefabricated scaffolds which cells are seeded. The self-assembly protocol recently produced organ-specific human-derived three-dimensional without need for exogenous material. This strategy will help achieve 3R principles.

Language: Английский

Citations

113
Recurrent Urinary Tract Infection: A Mystery in Search of Better Model Systems DOI Creative Commons
Benjamin Murray, Carlos Flores, Corin Williams

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2021, Volume and Issue: 11

Published: May 26, 2021

Urinary tract infections (UTIs) are among the most common infectious diseases worldwide but significantly understudied. Uropathogenic E. coli (UPEC) accounts for a significant proportion of UTI, large number other species can infect urinary tract, each which will have unique host-pathogen interactions with bladder environment. Given substantial economic burden UTI and its increasing antibiotic resistance, there is an urgent need to better understand pathophysiology – especially tendency relapse recur. Most models developed date use murine infection; few human-relevant exist. Of these, majority in vitro utilized cells static culture, needs be studied context aspects bladder’s biophysical environment (e.g., tissue architecture, urine, fluid flow, stretch). In this review, we summarize complexities recurrent critically assess current infection discuss potential improvements. More advanced human cell-based enable understanding etiology disease provide complementary platform alongside animals drug screening search treatments.

Language: Английский

Citations

95
Modeling the Human Body on Microfluidic Chips DOI
Sasan Jalili‐Firoozinezhad, Cláudia C. Miranda, Joaquim M. S. Cabral

et al.

Trends in biotechnology, Journal Year: 2021, Volume and Issue: 39(8), P. 838 - 852

Published: Feb. 10, 2021

Language: Английский

Citations

77
Simulating drug concentrations in PDMS microfluidic organ chips DOI
Jennifer Grant, Aliçan Özkan, Crystal Yuri Oh

et al.

Lab on a Chip, Journal Year: 2021, Volume and Issue: 21(18), P. 3509 - 3519

Published: Jan. 1, 2021

A simulation method for predicting drug responses is described that overcomes challenges relating to absorption of hydrophobic molecules by Organ Chips made from polydimethylsiloxane (PDMS).

Language: Английский

Citations

74