Bioprinting, Journal Year: 2023, Volume and Issue: 32, P. e00280 - e00280
Published: May 18, 2023
Language: Английский
Biomaterials, Journal Year: 2022, Volume and Issue: 287, P. 121639 - 121639
Published: June 20, 2022
Language: Английский
Citations
149
Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(2), P. 464 - 464
Published: Feb. 21, 2022
Skin tissue engineering and regeneration aim at repairing defective skin injuries progress in wound healing. Until now, even though several developments are made this field, it is still challenging to face the complexity of with current methods fabrication. In review, short, state-of-the-art on using 3D bioprinting as a new tool described. The summary bioink formulations, parameters, properties discussed. Finally, representative number examples advances field together limitations future needs provided.
Language: Английский
Citations
73
Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 168, P. 1 - 21
Published: July 15, 2023
3D-printed composite scaffolds have emerged as an alternative to deal with existing limitations when facing bone reconstruction. The aim of the study was systematically review feasibility using PLA/bioceramic manufactured by 3D-printing technologies grafting materials in preclinical vivo studies. Electronic databases were searched specific search terms, and thirteen manuscripts selected after screening. synthesis carried out mainly extrusion-based techniques. Likewise, hydroxyapatite most used bioceramic for synthesizing composites a PLA matrix. Among studies, seven conducted rats six rabbits, but high variability that exists regarding experimental process made it difficult compare them. Regarding results, PLA/Bioceramic shown be biocompatible mechanically resistant. Preclinical studies elucidated ability grafts, allowing growing without adverse reactions. In conclusion, PLA/Bioceramics been demonstrated promising treating defects. Nevertheless, more care should taken designing performing trials, since lack standardization processes, which prevents comparison results reduces quality information. polylactic acid/bioceramic Since animal models represent mandatory step clinical translation, present manuscript analyzed discussed not only those aspects related selection material, implants their characterization. But provides new approach understand how design perform well analysis methods, may affect obtained covering authors' knowledgebase from veterinary medicine biomaterial science. Thus, this aims trials.
Language: Английский
Citations
49
Stem Cell Reviews and Reports, Journal Year: 2024, Volume and Issue: 20(4), P. 881 - 899
Published: March 1, 2024
Language: Английский
Citations
27
Biotechnology Notes, Journal Year: 2024, Volume and Issue: 5, P. 8 - 12
Published: Jan. 1, 2024
Organ-on-chip (OOC) technology is an innovative approach that reproduces human organ structures and functions on microfluidic platforms, offering detailed insights into intricate physiological processes. This provides unique advantages over conventional in vitro vivo models thus has the potential to become new standard for biomedical research drug screening. In this mini-review, we compare OOCs with models, highlighting their differences, present several applications of research. Additionally, highlight advancements OOC technology, particularly developing multiorgan systems, discuss challenges future directions field.
Language: Английский
Citations
19
Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13
Published: July 22, 2022
Recently, research is undergoing a drastic change in the application of animal model as unique investigation strategy, considering an alternative approach for development science future. Although conventional monolayer cell cultures represent established and widely used vitro method, lack tissue architecture complexity such fails to inform true biological processes vivo . Recent advances culture techniques have revolutionized tools biomedical by creating powerful three-dimensional (3D) models recapitulate heterogeneity, structure functions primary tissues. These also bridge gap between traditional two-dimensional (2D) single-layer models. 3D systems allow researchers recreate human organs diseases one dish thus holds great promise many applications regenerative medicine, drug discovery, precision cancer research, gene expression studies. Bioengineering has made important contribution context using scaffolds that help mimic microenvironments which cells naturally reside, supporting mechanical, physical biochemical requirements cellular growth function. We therefore speak based on organoids, bioreactors, organ-on-a-chip up bioprinting each these provides its own advantages applications. All prove be excellent candidates methods testing, well revolutionizing technology. will able provide new ideas study interactions both basic more specialized compliance with 3R principle. In this review, we comparison 2D culture, details some different currently available discussing their strengths potential
Language: Английский
Citations
65
Bioengineering, Journal Year: 2022, Volume and Issue: 9(1), P. 28 - 28
Published: Jan. 11, 2022
Organ on chip (OOC) has emerged as a major technological breakthrough and distinct model system revolutionizing biomedical research drug discovery by recapitulating the crucial structural functional complexity of human organs in vitro. OOC are rapidly emerging powerful tools for oncology research. Indeed, Cancer (COC) can ideally reproduce certain key aspects tumor microenvironment (TME), such biochemical gradients niche factors, dynamic cell-cell cell-matrix interactions, complex tissue structures composed stromal cells. Here, we review state art COC models with focus microphysiological systems that host multicellular 3D engineering help elucidate biology TME cancer growth progression. Finally, some examples microengineered integrated multi-organ microdevices to study disease progression different tissues will be presented.
Language: Английский
Citations
54
Beni-Suef University Journal of Basic and Applied Sciences, Journal Year: 2022, Volume and Issue: 11(1)
Published: Jan. 4, 2022
Abstract Background Evolution in the vitro cell culture from conventional 2D to 3D technique has been a significant accomplishment. The models have provided close and better insight into physiological study of human body. increasing demand for organs like liver, kidney, pancreas transplantation, rapid anti-cancer drug screening, limitations associated with use animal attracted interest researchers explore organ culture. Main body Natural, synthetic, hybrid material-based hydrogels are being used as scaffolds provide 'close-to-in vivo’ structures. Organoids: stem cell-derived small size systems now favored due their ability mimic in-vivo conditions or tissue this characteristic made it eligible variety clinical applications, discovery regenerative medicine few many areas application. applications long-time ethical biological challenge get accurate outcomes. bioprinting resolved issue vascularization organoid great extent by its layer-by-layer construction approach. bioprinted organoids popular application personalized disease modeling development therapeutics. Short conclusions This review paper, focuses on discussing novel approach, advantages limitations, potential life science namely cancer research, therapy, engineering, discovery. Graphical
Language: Английский
Citations
54
Nanotechnology, Journal Year: 2024, Volume and Issue: 35(39), P. 392002 - 392002
Published: June 28, 2024
Tissue engineering is a multidisciplinary field that merges engineering, material science, and medical biology in order to develop biological alternatives for repairing, replacing, maintaining, or boosting the functionality of tissues organs. The ultimate goal tissue create enhancing However, current landscape techniques presents several challenges, including lack suitable biomaterials, inadequate cell proliferation, limited methodologies replicating desired physiological structures, unstable insufficient production growth factors, which are essential facilitating communication appropriate cellular responses. Despite these there has been significant progress made recent years. Nanoparticles hold major role within realm nanotechnology due their unique qualities change with size. These particles, provide potential solutions issues met have helped propel its state prominence. substantial breakthroughs utilization nanoparticles over past two decades, full range addressing difficulties remains largely untapped. This fact advancements occurred relatively isolated pockets. In purpose this research conduct an in-depth investigation ways various types might be put use. addition this, it sheds light on challenges need conquered unlock maximum area.
Language: Английский
Citations
9
Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1638 - 1638
Published: Oct. 1, 2024
The 3D bioprinting technique has made enormous progress in tissue engineering, regenerative medicine and research into diseases such as cancer. Apart from individual cells, a collection of organoids, can be printed combination with various hydrogels. It hypothesized that will even become promising tool for mechanobiological analyses organoids their matrix environments highly defined precisely structured environments, which the mechanical properties cell environment individually adjusted. Mechanical obstacles or bead markers integrated bioprinted samples to analyze deformations forces within these constructs, perform biophysical analysis complex systems, are still not standard techniques. review highlights advances 4D printing technologies integrating cues so next step detailed key future directions organoid generation development disease model regeneration drug testing perspective. Finally, hydrogels, pure natural synthetic hydrogels mixtures, organoid–cell co-cultures, organ-on-a-chip systems organoid-organ-on-a chip combinations introduces use assembloids determine mutual interactions different types cell–matrix interferences specific biological environments.
Language: Английский
Citations
9