Micromachines,
Journal Year:
2021,
Volume and Issue:
12(12), P. 1464 - 1464
Published: Nov. 27, 2021
The
deleterious
effects
of
the
coronavirus
disease
2019
(COVID-19)
pandemic
urged
development
diagnostic
tools
to
manage
spread
disease.
Currently,
“gold
standard”
involves
use
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR)
for
SARS-CoV-2
detection.
Even
though
it
is
sensitive,
specific
and
applicable
large
batches
samples,
qRT-PCR
labour-intensive,
time-consuming,
requires
trained
personnel
not
available
in
remote
settings.
This
review
summarizes
compares
strategies
COVID-19:
serological
testing,
Point-of-Care
Testing,
nanotechnology-based
approaches
biosensors.
Last
but
least,
we
address
advantages
limitations
these
methods
as
well
perspectives
COVID-19
diagnostics.
effort
constantly
focused
on
understanding
quickly
changing
landscape
testing
at
clinical
levels
introducing
reliable
rapid
screening
point
care
testing.
last
approach
key
aid
decision-making
process
infection
control,
enhancing
an
appropriate
treatment
strategy
prompt
isolation
asymptomatic/mild
cases.
As
a
viable
alternative,
Testing
(POCT)
typically
low-cost
user-friendly,
hence
harbouring
tremendous
potential
diagnosis.
Journal of Clinical Microbiology,
Journal Year:
2022,
Volume and Issue:
60(10)
Published: July 19, 2022
Nearly
40
years
have
elapsed
since
the
invention
of
PCR,
with
its
extremely
sensitive
and
specific
ability
to
detect
nucleic
acids
via
in
vitro
enzyme-mediated
amplification.
In
turn,
more
than
2
passed
onset
coronavirus
disease
2019
(COVID-19)
pandemic,
during
which
time
molecular
diagnostics
for
infectious
diseases
assumed
a
larger
global
role
ever
before.
Military Medical Research,
Journal Year:
2022,
Volume and Issue:
9(1)
Published: March 18, 2022
Abstract
Traditional
diagnostic
strategies
for
infectious
disease
detection
require
benchtop
instruments
that
are
inappropriate
point-of-care
testing
(POCT).
Emerging
microfluidics,
a
highly
miniaturized,
automatic,
and
integrated
technology,
potential
substitute
traditional
methods
in
performing
rapid,
low-cost,
accurate,
on-site
diagnoses.
Molecular
diagnostics
widely
used
microfluidic
devices
as
the
most
effective
approaches
pathogen
detection.
This
review
summarizes
latest
advances
microfluidics-based
molecular
diseases
from
academic
perspectives
industrial
outlooks.
First,
we
introduce
typical
on-chip
nucleic
acid
processes,
including
sample
preprocessing,
amplification,
signal
read-out.
Then,
four
categories
of
platforms
compared
with
respect
to
features,
merits,
demerits.
We
further
discuss
application
digital
assay
absolute
quantification.
Both
classic
recent
commercial
summarized
proof
current
market
status.
Finally,
propose
future
directions
diagnosis.
ECS Sensors Plus,
Journal Year:
2024,
Volume and Issue:
3(1), P. 011601 - 011601
Published: March 1, 2024
There
is
a
plethora
of
electrochemical
biosensors
developed
for
ultrasensitive
detection
clinically
relevant
biomarkers.
However,
many
these
systems
lose
their
performance
in
heterogeneous
clinical
samples
and
are
too
complex
to
be
operated
by
end
users
at
the
point-of-care
(POC),
prohibiting
commercial
success.
Integration
with
sample
processing
technology
addresses
both
challenges;
however,
it
adds
manufacturing
complexity
overall
cost
systems.
Herein,
we
review
different
components
biosensor
avenues
creating
fully
integrated
In
context
integration,
focus
on
discussing
trade-offs
between
sensing
performance,
cost,
scalable
guide
readers
toward
designing
new
commercialization
potential.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(16)
Published: Feb. 26, 2024
Bacterial
infections
have
emerged
as
the
leading
causes
of
mortality
and
morbidity
worldwide.
Herein,
we
developed
a
dual-channel
fluorescence
"turn-on"
sensor
array,
comprising
six
electrostatic
complexes
formed
from
one
negatively
charged
poly(para-aryleneethynylene)
(PPE)
positively
aggregation-induced
emission
(AIE)
fluorophores.
The
6-element
array
enabled
simultaneous
identification
20
bacteria
(OD
Biomicrofluidics,
Journal Year:
2022,
Volume and Issue:
16(2)
Published: March 1, 2022
Transforming
lab
research
into
a
sustainable
business
is
becoming
trend
in
the
microfluidic
field.
However,
there
are
various
challenges
during
translation
process
due
to
gaps
between
academia
and
industry,
especially
from
laboratory
prototyping
industrial
scale-up
production,
which
critical
for
potential
commercialization.
In
this
Perspective,
based
on
our
experience
collaboration
with
stakeholders,
e.g.,
biologists,
engineers,
diagnostic
specialists,
manufacturers,
we
aim
share
understanding
of
manufacturing
chain
cartridge
concept
development
where
production
commercial
cartridges
highlighted.
Four
suggestions
aspect
design
manufacturing,
professional
involvement,
material
selection,
standardization
provided
order
help
scientists
bring
their
innovations
pre-clinical,
clinical,
mass
improve
manufacturability
prototypes
toward
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(6), P. 584 - 584
Published: May 27, 2023
Conventional
diagnostic
techniques
are
based
on
the
utilization
of
analyte
sampling,
sensing
and
signaling
separate
platforms
for
detection
purposes,
which
must
be
integrated
to
a
single
step
procedure
in
point
care
(POC)
testing
devices.
Due
expeditious
nature
microfluidic
platforms,
trend
has
been
shifted
toward
implementation
these
systems
analytes
biochemical,
clinical
food
technology.
Microfluidic
molded
with
substances
such
as
polymers
or
glass
offer
specific
sensitive
infectious
noninfectious
diseases
by
providing
innumerable
benefits,
including
less
cost,
good
biological
affinity,
strong
capillary
action
simple
process
fabrication.
In
case
nanosensors
nucleic
acid
detection,
some
challenges
need
addressed,
cellular
lysis,
isolation
amplification
before
its
detection.
To
avoid
laborious
steps
executing
processes,
advances
have
deployed
this
perspective
on-chip
sample
preparation,
introduction
an
emerging
field
modular
microfluidics
that
multiple
advantages
over
microfluidics.
This
review
emphasizes
significance
technology
non-infectious
diseases.
The
isothermal
conjunction
lateral
flow
assay
greatly
increases
binding
efficiency
nanoparticles
biomolecules
improves
limit
sensitivity.
Most
importantly,
deployment
paper-based
material
made
cellulose
reduces
overall
cost.
discussed
explicating
applications
different
fields.
Next-generation
methods
can
improved
using
CRISPR/Cas
systems.
concludes
comparison
future
prospects
various
systems,
plasma
separation
used
ACS Nano,
Journal Year:
2023,
Volume and Issue:
17(7), P. 6507 - 6518
Published: March 27, 2023
Point-of-care
real-time
reverse-transcription
polymerase
chain
reaction
(RT-PCR)
facilitates
the
widespread
use
of
rapid,
accurate,
and
cost-effective
near-patient
testing
that
is
available
to
public.
Here,
we
report
ultrafast
plasmonic
nucleic
acid
amplification
quantification
for
decentralized
molecular
diagnostics.
The
RT-PCR
system
features
an
thermocycler
(PTC),
a
disposable
plastic-on-metal
(PoM)
cartridge,
ultrathin
microlens
array
fluorescence
(MAF)
microscope.
PTC
provides
photothermal
cycling
under
white-light-emitting
diode
illumination
precise
temperature
monitoring
with
integrated
resistance
detector.
PoM
thin
film
cartridge
allows
rapid
heat
transfer
as
well
complete
light
blocking
from
excitation
source,
resulting
in
highly
efficient
PCR
quantification.
Besides,
MAF
microscope
exhibits
close-up
high-contrast
microscopic
imaging.
All
systems
were
fully
packaged
palm
size
point-of-care
testing.
demonstrates
diagnosis
coronavirus
disease-19
RNA
virus
within
10
min
yields
95.6%
efficiency,
96.6%
classification
accuracy
preoperational
test,
91%
total
percent
agreement
clinical
diagnostic
test.
compact
can
decentralize
primary
care
developing
countries.
