DNA‐Based Nanomaterials for Analysis of Extracellular Vesicles DOI
Jinqi Deng, Chao Liu, Jiashu Sun

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(22)

Published: Nov. 28, 2023

Abstract Extracellular vesicles (EVs) are cell‐derived nanovesicles comprising a myriad of molecular cargo such as proteins and nucleic acids, playing essential roles in intercellular communication physiological pathological processes. EVs have received substantial attention noninvasive biomarkers for disease diagnosis prognosis. Owing to their ability recognize protein acid targets, DNA‐based nanomaterials with excellent programmability modifiability provide promising tool the sensitive accurate detection carried by EVs. In this perspective, recent advancements EV analysis using variety summarized, which can be broadly classified into three categories: linear DNA probes, nanostructures, hybrid nanomaterials. The design, construction, advantages, disadvantages different types nanomaterials, well performance detecting reviewed. challenges opportunities field also discussed.

Language: Английский

Recent advances in cascade isothermal amplification techniques for ultra-sensitive nucleic acid detection DOI Open Access
Hao Jiang, Yuan Li,

Xuefei Lv

et al.

Talanta, Journal Year: 2023, Volume and Issue: 260, P. 124645 - 124645

Published: May 4, 2023

Language: Английский

Citations

44
Enzyme-free isothermal amplification strategy for the detection of tumor-associated biomarkers: A review DOI
Wenjia Wang, Qinyu Ge,

Xiangwei Zhao

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 160, P. 116960 - 116960

Published: Feb. 4, 2023

Language: Английский

Citations

32
DNA Nanomaterial-Based Electrochemical Biosensors for Clinical Diagnosis DOI

Mengge Chu,

Yawen Zhang,

Cailing Ji

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(46), P. 31713 - 31736

Published: Nov. 7, 2024

Sensitive and quantitative detection of chemical biological molecules for screening, diagnosis monitoring diseases is essential to treatment planning response monitoring. Electrochemical biosensors are fast, sensitive, easy miniaturize, which has led rapid development in clinical diagnosis. Benefiting from their excellent molecular recognition ability high programmability, DNA nanomaterials could overcome the Debye length electrochemical by simple design well suited as elements biosensors. Therefore, enhance sensitivity specificity biosensors, significant progress been made recent years optimizing design. Here, establishment sensing strategies based on reviewed detail. First, structural nanomaterial examined improving overcoming length. In addition, electrical signal transduction amplification reviewed, applications nanomaterial-based integrated devices further summarized. Finally, main opportunities challenges detecting disease biomarkers presented an aim guide with specificity.

Language: Английский

Citations

14
Improved surface-enhanced Raman scattering detection of miRNA employing polyhedral gold plasmonic nanoparticles in conjunction with DNA cascade amplification strategy DOI

Yufei Zang,

Xu Yang, Lin Zhao

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137320 - 137320

Published: Jan. 1, 2025

Language: Английский

Citations

2
Paper-based electrochemiluminescence telomerase activity detection using hybridization chain reaction and CRISPR/Cas12a dual signal amplification DOI
Yun Zhang, Liang Gao,

Zijun Shi

et al.

Bioelectrochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108916 - 108916

Published: Jan. 1, 2025

Language: Английский

Citations

1
Integration of biodegradable nanosphere-mediated spatial-localized cascade catalytic hairpin assembly with quantum dots for intracellular circular RNA imaging and clinical diagnosis DOI

Huimin Yuan,

Qi-qin Ge,

Jinping Hu

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160685 - 160685

Published: Feb. 1, 2025

Language: Английский

Citations

1
Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens DOI Creative Commons
Jiangshan Wang, Josiah Levi Davidson, Simerdeep Kaur

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(12), P. 1094 - 1094

Published: Nov. 29, 2022

Paper-based biosensors are microfluidic analytical devices used for the detection of biochemical substances. The unique properties paper-based biosensors, including low cost, portability, disposability, and ease use, make them an excellent tool point-of-care testing. Among all analyte methods, nucleic acid-based pathogen offers versatility due to acid synthesis. In a testing context, combination platform allows accurate detection. This review overview contemporary detecting acids from pathogens. methods limitations implementing integrated portable discussed. concludes with potential directions future research in development biosensors.

Language: Английский

Citations

35
Cascade DNA Circuits Mediated CRISPR‐Cas12a Fluorescent Aptasensor based on Multifunctional Fe3O4@hollow‐TiO2@MoS2 Nanochains for Tetracycline Determination DOI
Yan Lv,

Yuhan Sun,

You Zhou

et al.

Small, Journal Year: 2023, Volume and Issue: 19(16)

Published: Jan. 22, 2023

Herein, for the first time, CRISPR-Cas12a system is combined with aptamer, cascaded dynamic DNA network circuits, and Fe3 O4 @hollow-TiO2 @MoS2 nanochains (Fe3 @h-TiO2 NCs) to construct an efficient sensing platform tetracycline (TC) analysis. In this strategy, specific recognition of target transduced amplified into H1-H2 duplexes containing sequence Cas12a-crRNA through aptamer module dual amplification network. Subsequently, obtained activated Cas12a protein non-specifically cleaves adjacent reporter gene ssDNA-FAM dissociate FAM molecule from quencher NCs, resulting in recovery fluorescence signal further amplification. Particularly, synthesized multifunctional NCs composites also exhibit superb magnetic separability photocatalytic degradation ability. Under optimal conditions, aptasensor displays a distinct linear relationship logarithm TC concentration, limit detection as low 0.384 pg mL-1 . Furthermore, results spiked confirm viability proposed quantification real samples. This study extends application field analytical contributes new insights exploration reliable tools monitoring treating hazards food environment.

Language: Английский

Citations

21
Integrating CRISPR/Cas12a with strand displacement amplification for the ultrasensitive aptasensing of cadmium(II) DOI
Xiaochen Ma,

Tiying Suo,

Furong Zhao

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2023, Volume and Issue: 415(12), P. 2281 - 2289

Published: March 23, 2023

Language: Английский

Citations

20
Visualized Lateral Flow Assay for Dual Viral RNA Fragment Detection DOI
Juanjuan Song,

Liangwen Zhang,

Luhao Zeng

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(30), P. 11187 - 11192

Published: July 18, 2023

In this technical note, we report an easy-to-produce, reverse-transcription-free, and protein-enzyme-free lateral flow assay for detection of viral RNA fragments by taking SARS-CoV-2 ORF1ab N as target models. Catalytic hairpin assembly is utilized dual fragment orthogonal reaction to generate copious amounts opened duplexes, which bridge DNA-modified gold nanoparticles capture strands on the strip induce coloration. The are simultaneously visualized during one time sample flow, single-base-mismatched nontarget sequences can be differentiated. test flexibly adapted detect evolutional variants such Delta Omicron. It also shows potential in visually detecting long-sequence virus simulants achieves a sensitivity comparable that RT-qPCR incorporation with upstream amplification. should offer convenient reliable technique nucleic acid detection.

Language: Английский

Citations

19