Probing Single-Particle Electrocatalytic Stability: Electrogenerated Chemiluminescence Imaging of Nanoparticle Array DOI

Zhouzhou Zhu,

Xiangfu Hu,

Ying Wei

et al.

The Journal of Physical Chemistry Letters, Journal Year: 2024, Volume and Issue: unknown, P. 12228 - 12233

Published: Dec. 5, 2024

Understanding the stability of single nanoparticles is crucial for optimizing their performance in various applications, including catalysis. In this study, we employed electrochemiluminescence (ECL) imaging to investigate temporal individual Au and Pt within precisely engineered arrays. Our results reveal significant differences NPs. While both exhibit initial decay due diffusion limitations, NPs undergo more rapid degradation, attributed surface oxidation detachment. contrast, demonstrate much better with little oxidation. This study provides valuable insights into fundamental behavior single-NP electrocatalysis highlights potential ECL as a powerful tool unraveling complex dynamics nanoscale systems.

Language: Английский

Chromatin structure and dynamics: one nucleosome at a time DOI
Diego M. Presman,

Belén Benítez,

Agustina L. Lafuente

et al.

Histochemistry and Cell Biology, Journal Year: 2024, Volume and Issue: 162(1-2), P. 79 - 90

Published: April 12, 2024

Language: Английский

Citations

8
Optics Determines the Electrochemiluminescence Signal of Bead-Based Immunoassays DOI
Dongni Han, Dechen Jiang, Giovanni Valenti

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(12), P. 4782 - 4791

Published: Nov. 17, 2023

Electrochemiluminescence (ECL) is an optical readout technique that successfully applied for the detection of biomarkers in body fluids using microbead-based immunoassays. This technology utmost importance vitro diagnostics and thus a very active research area but mainly focused on quest new dyes coreactants, whereas investigation ECL optics extremely scarce. Herein, we report 3D imaging signals recorded at single microbeads decorated with labels sandwich immunoassay format. We show effects due to light propagation through bead determine spatial distribution signals. Indeed, simulations based discrete dipole approximation compute rigorously electromagnetic scattering emission by microbead allow reconstructing map emission. Thus, it provides global description chemical reactivity associated optics. The outcomes this approach complemented modeling provide insight into unique mechanism operating bead-based Therefore, opens directions mechanistic investigations, ultrasensitive bioassays, imaging.

Language: Английский

Citations

15
Tris(2,2’-bipyridyl)ruthenium (II) complex as a universal reagent for the fabrication of heterogeneous electrochemiluminescence platforms and its recent analytical applications DOI

Mathavan Sornambigai,

Laurent Bouffier, Nešo Šojić

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2023, Volume and Issue: 415(24), P. 5875 - 5898

Published: July 29, 2023

Language: Английский

Citations

13
Electrogenerated chemiluminescence at boron-doped diamond electrodes DOI Creative Commons
Andrea Fiorani, Giovanni Valenti, Francesco Paolucci

et al.

Chemical Communications, Journal Year: 2023, Volume and Issue: 59(51), P. 7900 - 7910

Published: Jan. 1, 2023

Electrogenerated chemiluminescence (ECL) refers to the phenomenon of light emission from molecular species which is triggered by an electrochemical reaction. Therefore, like most systems, electrode material plays a pivotal role and much effort has been made in order find best for ECL, terms signal intensity long-term stability, especially after development ECL analytical applications. In this article, we will introduce highlight distinctive features boron-doped diamond (BDD) as complementary properties compared common metals (e.g., Au or Pt) carbon materials glassy carbon, nanotubes graphene). Boron-doped electrodes emerged novel electrodes, gaining more interest community their peculiar characteristics such wide solvent window, low capacitance, resistance fouling mechanical robustness. Furthermore, metal BDD does not form oxide layer aqueous solutions, sp3 hybridization gives ability enable reactions that are possible on sp2 materials. investigations with have reported systems (luminophores co-reactants), special only includes situ generation co-reactant.

Language: Английский

Citations

12
CRISPR/Cas12a-Responsive Smart DNA Hydrogel for Sensitive Electrochemiluminescence Detection of the Huanglongbing Outer Membrane Protein Gene DOI
Yutian Zhang,

Can Hu,

Yashi Yin

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11611 - 11618

Published: June 29, 2024

Citrus Huanglongbing (HLB) is known as the cancer of citrus, where

Language: Английский

Citations

5
Immunosensor with Enhanced Electrochemiluminescence Signal Using Platinum Nanoparticles Confined within Nanochannels for Highly Sensitive Detection of Carcinoembryonic Antigen DOI Creative Commons
Huihua Zhang, Chaoyan Zhang, Hui Qu

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(18), P. 6559 - 6559

Published: Sept. 11, 2023

Rapid, highly sensitive, and accurate detection of tumor biomarkers in serum is great significance cancer screening, early diagnosis, postoperative monitoring. In this study, an electrochemiluminescence (ECL) immunosensing platform was constructed by enhancing the ECL signal through situ growth platinum nanoparticles (PtNPs) a nanochannel array, which can achieve sensitive marker carcinoembryonic antigen (CEA). An inexpensive readily available indium tin oxide (ITO) glass electrode used as supporting electrode, layer amino-functionalized vertically ordered mesoporous silica film (NH2-VMSF) grown on its surface using electrochemically assisted self-assembly method (EASA). The amino groups within nanochannels served anchoring sites for one-step electrodeposition PtNPs, taking advantage confinement effect ultrasmall nanochannels. After outer NH2-VMSF were derivatized with aldehyde groups, specific recognition antibodies covalently immobilized followed blocking nonspecific binding to create immunorecognition interface. acting nanocatalysts, catalyzed generation reactive oxygen species (ROS) hydrogen peroxide (H2O2), significantly luminol. exhibited high stability during continuous electrochemical scanning. When CEA specifically bound interface, resulting immune complexes restricted diffusion emitters co-reactants towards leading reduction signal. Based recognition-induced signal-gating effect, immunosensor enabled linear range 0.1 pg mL-1 1000 ng low limit (LOD, 0.03 mL-1). demonstrated excellent selectivity serum.

Language: Английский

Citations

11
A review of innovative electrochemical strategies for bioactive molecule detection and cell imaging: Current advances and challenges DOI
Hao Zhang, Hui Jiang, Xiaohui Liu

et al.

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1285, P. 341920 - 341920

Published: Oct. 19, 2023

Language: Английский

Citations

10
Infrared photoinduced electrochemiluminescence microscopy of single cells DOI Creative Commons
Julie Descamps, Yiran Zhao, Bertrand Goudeau

et al.

Chemical Science, Journal Year: 2023, Volume and Issue: 15(6), P. 2055 - 2061

Published: Dec. 8, 2023

Electrochemiluminescence (ECL) is evolving rapidly from a purely analytical technique into powerful microscopy. Herein, we report the imaging of single cells by photoinduced ECL (PECL; λem = 620 nm) stimulated an incident near-infrared light (λexc 1050 nm). The were grown on metal-insulator-semiconductor (MIS) n-Si/SiOx/Ir photoanode that exhibited stable and bright PECL emission. large anti-Stokes shift allowed for recording well-resolved images with high sensitivity. microscopy demonstrated at remarkably low onset potential 0.8 V; this contrasts classic ECL, which blind potential. Two modes are reported: (i) positive (PECL+), showing cell membranes labeled [Ru(bpy)3]2+ complex; (ii) shadow label-free (PECL-) morphology, luminophore in solution. Finally, adding new dimension stimulus, should find promising applications to image study photoactive nanoparticles biological entities.

Language: Английский

Citations

10
Simultaneous determination of α-fetoprotein and carbohydrate antigens 125 and 19–9 in potential- and spectrum-resolved dual-mode electrochemiluminescence DOI

Junying Ge,

Jianzhi Dou,

Xifeng Yu

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 199, P. 110117 - 110117

Published: Feb. 7, 2024

Language: Английский

Citations

4
Recent Advances in Electrochemiluminescence Visual Biosensing and Bioimaging DOI

Yajuan Yan,

Lurong Ding,

Jialian Ding

et al.

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 2, 2024

Electrochemiluminescence (ECL) is one of the most powerful techniques that meet needs analysis and detection in a variety scenarios, because its highly analytical sensitivity excellent spatiotemporal controllability. ECL combined with microscopy (ECLM) offers promising approach for quantifying mapping wide range analytes. To date, ECLM has been widely used to image biological entities processes, such as cells, subcellular structures, proteins membrane transport properties. In this review, we first introduced mechanisms several classic systems, then highlighted progress visual biosensing bioimaging by last decade. Finally, characteristics were summarized, well some current challenges. The future research interests potential directions application also outlooked.

Language: Английский

Citations

4