Metal–Organic‐Framework‐Engineered Enzyme‐Mimetic Catalysts

Advanced Materials, Journal Year: 2020, Volume and Issue: 32(49)

Published: Oct. 30, 2020

Nanomaterial-based enzyme-mimetic catalysts (Enz-Cats) have received considerable attention because of their optimized and enhanced catalytic performances selectivities in diverse physiological environments compared with natural enzymes. Recently, owing to molecular/atomic-level centers, high porosity, large surface area, loading capacity, homogeneous structure, metal–organic frameworks (MOFs) emerged as one the most promising materials engineering Enz-Cats. Here, recent advances design MOF-engineered Enz-Cats, including preparation methods, composite constructions, structural characterizations, biomedical applications, are highlighted commented upon. In particular, performance, selectivities, essential mechanisms, potential structure–property relations these Enz-Cats accelerating reactions discussed. Some applications also breifly proposed. These include, for example, tumor therapies, bacterial disinfection, tissue regeneration, biosensors. Finally, future opportunities challenges emerging research frontiers thoroughly Thereby, pathways perspectives designing state-of-the-art sciences offered.

Language: Английский

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Versatile polyphenolic platforms in regulating cell biology

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(10), P. 4175 - 4198

Published: Jan. 1, 2022

Polyphenolic materials are a class of fascinating and versatile bioinspired for biointerfacial engineering. In particular, due to the presence active chemical groups, series unique physicochemical properties become accessible tunable as-prepared polyphenolic platforms, which could delicately regulate cell activities via cell-material contact-dependent interactions. More interestingly, polyphenols also affect behaviors contact-independent manner, arise their intrinsically functional characteristics (e.g., antioxidant photothermal behaviors). As such, comprehensive understanding on relationship between material desired biomedical applications, as well underlying mechanism at cellular molecular level would provide design principles accelerate lab-to-clinic translation platforms. this review, we firstly give brief overview hallmarks governed by surrounding cues, followed introduction engineering strategies. Subsequently, detailed discussion cell-polyphenols interfacial interaction was carefully provided. Lastly, applications were elaborated. We believe that review guidances rational multifunctional extend application window.

Language: Английский

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Smart polydopamine-based nanoplatforms for biomedical applications: state-of-art and further perspectives

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 488, P. 215153 - 215153

Published: April 27, 2023

Language: Английский

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3D printing of bio-instructive materials: Toward directing the cell

Bioactive Materials, Journal Year: 2022, Volume and Issue: 19, P. 292 - 327

Published: April 23, 2022

Fabrication of functional scaffolds for tissue engineering and regenerative medicine applications requires material systems with precise control over cellular performance. 3D printing is a powerful technique to create highly complex multicomponent structures well-defined architecture composition. In this review paper, we explore extrusion-based methods (EBP, i.e., Near Field Electrospinning (NFES), Melt Electrowriting (MEW), Fused Deposition Modeling (FDM), extrusion bioprinting) in terms their ability produce bio-instructive properties. These provide spatio-temporal guidance cells, allowing controlled regeneration maturation. Multiple physical biochemical cues introduced the EBP are evaluated direct cell alignment, proliferation, differentiation, specific ECM production, We indicate that have different impacts depending on system, type used, or coexistence multiple cues. Therefore, they must be carefully chosen based targeted application. propose future directions materials development, including such concepts as metamaterials, hybrid living materials, 4D printing. The gathers knowledge essential designing new response, fabrication advanced engineered tissue, developing better understanding biology, especially response biomaterial.

Language: Английский

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Static and Dynamic: Evolving Biomaterial Mechanical Properties to Control Cellular Mechanotransduction

Advanced Science, Journal Year: 2023, Volume and Issue: 10(9)

Published: Jan. 19, 2023

The extracellular matrix (ECM) is a highly dynamic system that constantly offers physical, biological, and chemical signals to embraced cells. Increasing evidence suggests mechanical derived from the cellular microenvironment are essential controllers of cell behaviors. Conventional culture biomaterials, with static properties such as chemistry, topography, stiffness, have offered fundamental understanding various vital biochemical biophysical processes, adhesion, spreading, migration, growth, differentiation. At present, novel biomaterials can spatiotemporally impart cues manipulate fate emerging. adaptive traits new materials endow them ability adapt requirements enhance functions. In this review, an introductory overview key players mechanobiology provided. A perspective on state-of-the-art manipulation techniques in designing ECM-mimicking taken. particular, different regulating mechanosensing functions compared. This review benefit development engineering biomechanical systems expected.

Language: Английский

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3D-printed tri-element-doped hydroxyapatite/ polycaprolactone composite scaffolds with antibacterial potential for osteosarcoma therapy and bone regeneration

Bioactive Materials, Journal Year: 2023, Volume and Issue: 31, P. 18 - 37

Published: Aug. 4, 2023

The resection of malignant osteosarcoma often results in large segmental bone defects, and the residual cells can facilitate recurrence. Consequently, treatment is a major challenge clinical practice. ideal goal for to eliminate it thoroughly, repair resultant defects as well avoid bacterial infections. Herein, we fabricated selenium/strontium/zinc-doped hydroxyapatite (Se/Sr/Zn-HA) powder by hydrothermal method, then employed with polycaprolactone (PCL) ink construct composite scaffolds through 3D printing, finally introduced them defect induced osteosarcoma. integrated multiple functions involving anti-tumor, osteogenic, antibacterial potentials, mainly attributed anti-tumor effects SeO32−, osteogenic Sr2+ Zn2+, SeO32− Zn2+. In vitro studies confirmed that Se/Sr/Zn-HA leaching solution could induce apoptosis cells, differentiation MSCs, proliferation MC3T3-E1 while showing excellent properties. vivo tests demonstrated significantly suppress tumors after 8 days injection, Se/Sr/Zn-HA-PCLs scaffold repaired femoral effectively 3 months implantation. Summarily, developed this study were effective tumor treatment, repair, post-operative anti-infection, which provided great potential be facile therapeutic material resection.

Language: Английский

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Extracellular Matrix Cues Regulate Mechanosensing and Mechanotransduction of Cancer Cells

Cells, Journal Year: 2024, Volume and Issue: 13(1), P. 96 - 96

Published: Jan. 2, 2024

Extracellular biophysical properties have particular implications for a wide spectrum of cellular behaviors and functions, including growth, motility, differentiation, apoptosis, gene expression, cell–matrix cell–cell adhesion, signal transduction mechanotransduction. Cells not only react to unambiguously mechanical cues from the extracellular matrix (ECM), but can occasionally manipulate features in parallel with biological characteristics, thus interfering downstream matrix-based both physiological pathological processes. Bidirectional interactions between cells (bio)materials vitro alter cell phenotype mechanotransduction, as well ECM structure, intentionally or unintentionally. Interactions mechanics vivo are importance variety diseases, primarily cancer. Stiffness values normal cancerous tissue range 500 Pa (soft) 48 kPa (stiff), respectively. Even shear flow increase 0.1–1 dyn/cm2 (normal tissue) 1–10 (cancerous tissue). There currently many new areas activity tumor research on various length scales, which highlighted this review. Moreover, complexity cancer is reduced common different tumors characteristics identify main pathways interaction. This all contributes standardization mechanotransduction models approaches, which, ultimately, increases understanding complex Finally, effects mechanics–biology pairing key insights clinical practice treatment and, consequently, translation.

Language: Английский

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Fiber Flexibility Reconciles Matrix Recruitment and the Fiber Modulus to Promote Cell Mechanosensing

Nano Letters, Journal Year: 2024, Volume and Issue: 24(13), P. 4029 - 4037

Published: March 25, 2024

The mechanical interaction between cells and the extracellular matrix is pervasive in biological systems. On fibrous substrates, possess ability to recruit neighboring fibers, thereby augmenting their own adhesion facilitating generation of cues. However, matrices with high moduli impede fiber recruitment, restricting cell mechanoresponse. Herein, by harnessing inherent swelling properties gelatin, flexible gelatin methacryloyl network empowers fibers spanning a broad spectrum physiological during adhesion. flexibility concurrently facilitates optimization distribution, deformability, modulus, contributing promotion mechanosensing. Consequently, randomly distributed maximize adhesive forces. This study uncovers impact recruitment on mechanosensing introduces as previously unexplored property, offering an innovative perspective for design development novel biomaterials.

Language: Английский

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Aligned PCL Fiber Conduits Immobilized with Nerve Growth Factor Gradients Enhance and Direct Sciatic Nerve Regeneration

Advanced Functional Materials, Journal Year: 2020, Volume and Issue: 30(39)

Published: Aug. 13, 2020

Abstract Topographical guidance and chemotaxis are crucial factors for peripheral nerve regeneration. This study describes the preparation of highly aligned poly(ε‐caprolactone) (PCL) fiber conduits coated with a concentration gradient growth factor (NGF) (A/G‐PCL) using newly designed electrospinning receiving device. The A/G‐PCL confirmed in vitro to enhance attract neurite longitudinal dorsal root ganglion (DRG) neurons toward their high‐concentration side. In vivo, observed direct stronger attraction axons migration Schwann cells 15 mm rat sciatic defects. At 12 weeks, rats transplanted show satisfactory morphological functional improvements g‐ratio, total number, area myelinated fibers as well function index, compound muscle action potentials, wet weight ratio compared PCL uniform NGF (A/U‐PCL). performance is similar that autografts. Moreover, mRNA‐seq RT‐PCR results reveal Rap1, MAPK, cell adhesion molecules signaling pathways closely associated axon chemotactic response attraction. Altogether, by combining structural chemotaxis, NGF‐gradient/aligned represent promising approach defect repair.

Language: Английский

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BMP‐2 Signaling and Mechanotransduction Synergize to Drive Osteogenic Differentiation via YAP/TAZ

Advanced Science, Journal Year: 2020, Volume and Issue: 7(15)

Published: June 16, 2020

Abstract Growth factors and mechanical cues synergistically affect cellular functions, triggering a variety of signaling pathways. The molecular levels such cooperative interactions are not fully understood. Due to its role in osteogenesis, the growth factor bone morphogenetic protein 2 (BMP‐2) is tremendous interest for regenerative medicine, osteoporosis therapeutics, beyond. Here, contribution BMP‐2 extracellular osteogenic commitment C2C12 cells investigated. It revealed that these two distinct pathways integrated at transcriptional level provide multifactorial control cell differentiation. activation genes requires cooperation pathway‐associated Smad1/5/8 heteromeric complexes mechanosensitive YAP/TAZ translocation. further demonstrated Smad remain bound onto active on target genes, even after removal, suggesting they act as “molecular memory unit.” Thus, synergistic stimulation with drives differentiation programmable fashion.

Language: Английский

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