Biofabrication,
Journal Year:
2023,
Volume and Issue:
16(1), P. 015005 - 015005
Published: Oct. 11, 2023
Abstract
Recent
developments
in
digital
light
processing
(DLP)
can
advance
the
structural
and
biochemical
complexity
of
perfusable
vitro
models
blood–brain
barrier.
Here,
we
describe
a
strategy
to
functionalize
complex,
DLP-printed
vascular
with
multiple
peptide
motifs
single
hydrogel.
Different
peptides
be
clicked
into
walls
distinct
topologies,
or
lining
channel
differ
from
those
bulk
The
flexibility
this
approach
is
used
both
characterize
effects
various
bioactive
domains
on
endothelial
coverage
tight
junction
formation,
addition
facilitating
astrocyte
attachment
hydrogel
surrounding
endothelialized
vessel
mimic
endothelial–astrocyte
interaction.
Peptides
derived
proteins
mediating
cell-extracellular
matrix
(e.g.
RGD
IKVAV)
cell–cell
HAVDI)
adhesions
are
mediate
cell
coverage.
HAVDI
IKVAV-lined
channels
exhibit
significantly
greater
endothelialization
increased
zonula-occluden-1
(ZO-1)
localization
junctions
cells,
indicative
formation.
then
create
an
co-culture
model
barrier
that
overcomes
limitations
previous
platforms
incapable
complex
topology
tunable
domains.
This
yields
adjustable,
biofabricated
platform
interrogate
cell-matrix
interaction
mechanobiology.
Bioactive Materials,
Journal Year:
2023,
Volume and Issue:
31, P. 475 - 496
Published: Sept. 9, 2023
In
the
human
body,
almost
all
cells
interact
with
extracellular
matrices
(ECMs),
which
have
tissue
and
organ-specific
compositions
architectures.
These
ECMs
not
only
function
as
cellular
scaffolds,
providing
structural
support,
but
also
play
a
crucial
role
in
dynamically
regulating
various
functions.
This
comprehensive
review
delves
into
examination
of
biofabrication
strategies
used
to
develop
bioactive
materials
that
accurately
mimic
one
or
more
biophysical
biochemical
properties
ECMs.
We
discuss
potential
integration
these
ECM-mimics
range
physiological
pathological
vitro
models,
enhancing
our
understanding
behavior
organization.
Lastly,
we
propose
future
research
directions
for
context
engineering
organ-on-a-chip
applications,
offering
advancements
therapeutic
approaches
improved
patient
outcomes.
Polymers,
Journal Year:
2023,
Volume and Issue:
15(19), P. 3940 - 3940
Published: Sept. 29, 2023
Vat
photopolymerization
(VP),
including
stereolithography
(SLA),
digital
light
processing
(DLP),
and
volumetric
printing,
employs
UV
or
visible
to
solidify
cell-laden
photoactive
bioresin
contained
within
a
vat
in
point-by-point,
layer-by-layer,
manner.
VP-based
bioprinting
has
garnered
substantial
attention
both
academia
industry
due
its
unprecedented
control
over
printing
resolution
accuracy,
as
well
rapid
speed.
It
holds
tremendous
potential
for
the
fabrication
of
tissue-
organ-like
structures
field
regenerative
medicine.
This
review
summarizes
recent
progress
VP
fields
tissue
engineering
First,
it
introduces
mechanism
photopolymerization,
followed
by
an
explanation
technique
commonly
used
biomaterials.
Furthermore,
application
was
discussed.
Finally,
challenges
facing
are
discussed,
future
trends
projected.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
34(36)
Published: April 18, 2024
Abstract
Decellularized
small
intestine
submucosa
(dSIS)
is
a
promising
biomaterial
for
promoting
tissue
regeneration.
Isolated
from
the
submucosal
layer
of
animal
jejunum,
SIS
rich
in
extracellular
matrix
(ECM)
proteins,
including
collagen,
laminin,
and
fibronectin.
Following
mild
decellularization,
dSIS
becomes
an
acellular
that
supports
cell
adhesion,
proliferation,
differentiation.
Conventional
usually
obtained
by
thermal
crosslinking,
which
yields
soft
scaffold
with
low
stability.
To
address
these
challenges,
modified
methacrylate
groups
photocrosslinking
into
stable
hydrogels.
However,
has
not
been
clickable
handles
orthogonal
crosslinking.
Here,
development
norbornene‐modified
dSIS,
named
dSIS‐NB,
via
reacting
amine
carbic
anhydride
acidic
aqueous
reaction
conditions
reported.
Using
triethylamine
(TEA)
as
base
catalyst,
high
degrees
NB
substitution
on
are
obtained.
In
addition
to
describing
synthesis
its
adaptability
hydrogel
crosslinking
cancer
vascular
engineering
explored.
Impressively,
compared
physically
crosslinked
collagen
matrices,
orthogonally
dSIS‐NB
hydrogels
supported
rapid
dissemination
cells
superior
vasculogenic
angiogenic
properties.
also
exploited
versatile
bioink
3D
bioprinting.
Macromolecular Bioscience,
Journal Year:
2023,
Volume and Issue:
23(12)
Published: Aug. 3, 2023
Digital
light
processing
(DLP)
bioprinting
can
be
used
to
fabricate
volumetric
scaffolds
with
intricate
internal
structures,
such
as
perfusable
vascular
channels.
The
successful
implementation
of
DLP
in
tissue
fabrication
requires
using
suitable
photo-reactive
bioinks.
Norbornene-based
bioinks
have
emerged
an
attractive
alternative
(meth)acrylated
macromers
3D
owing
their
mild
and
rapid
reaction
kinetics,
high
cytocompatibility
for
situ
cell
encapsulation,
adaptability
post-printing
modification
or
conjugation
bioactive
motifs.
In
this
contribution,
the
development
gelatin-norbornene
(GelNB)
is
reported
a
photo-cross-linkable
bioink
bioprinting.
Low
concentrations
GelNB
(2-5
wt.%)
poly(ethylene
glycol)-tetra-thiol
(PEG4SH)
are
DLP-printed
wide
range
stiffness
(G'
≈120
4000
Pa)
hydrogels
highly
cytocompatible,
demonstrated
by
viability
encapsulated
human
umbilical
vein
endothelial
cells
(HUVECs).
HUVECs
formed
interconnected
microvascular
network
lumen
structures.
Notably,
permitted
both
tethering
secondary
QK
peptide,
growth
factor
(VEGF)-mimetic
peptide.
Incorporation
peptide
significantly
improved
endothelialization
vasculogenesis
hydrogels,
reinforcing
applicability
system
diverse
biofabrication
applications.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 17, 2024
Advancements
in
bioprinting
technology
are
driving
the
creation
of
complex,
functional
tissue
constructs
for
use
engineering
and
regenerative
medicine.
Various
methods,
including
extrusion,
jetting,
light-based
bioprinting,
have
their
unique
advantages
drawbacks.
Over
years,
researchers
industry
leaders
made
significant
progress
enhancing
techniques
materials,
resulting
production
increasingly
sophisticated
constructs.
Despite
this
progress,
challenges
still
need
to
be
addressed
achieving
clinically
relevant,
human-scale
constructs,
presenting
a
hurdle
widespread
clinical
translation.
However,
with
ongoing
interdisciplinary
research
collaboration,
field
is
rapidly
evolving
holds
promise
personalized
medical
interventions.
Continued
development
refinement
technologies
potential
address
complex
needs,
enabling
functional,
transplantable
tissues
organs,
as
well
advanced
vitro
models.
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 5, 2024
Abstract
3D
printing,
also
known
as
additive
manufacturing,
holds
immense
potential
for
rapid
prototyping
and
customized
production
of
functional
health‐related
devices.
With
advancements
in
polymer
chemistry
biomedical
engineering,
polymeric
biomaterials
have
become
integral
to
3D‐printed
applications.
However,
there
still
exists
a
bottleneck
the
compatibility
with
different
printing
methods,
well
intrinsic
challenges
such
limited
resolution
rates.
Therefore,
this
review
aims
introduce
current
state‐of‐the‐art
It
begins
an
overview
landscape
techniques,
followed
by
examination
commonly
used
biomaterials.
Subsequently,
examples
devices
are
provided
classified
into
categories
biosensors,
bioactuators,
soft
robotics,
energy
storage
systems,
self‐powered
devices,
data
science
bioplotting.
The
emphasis
is
on
exploring
capabilities
manufacturing
desired
geometries
that
facilitate
device
functionality
studying
reasons
material
choice.
Finally,
outlook
possible
improvements
near
future
presented,
projecting
contribution
general
field
healthcare.
