ACS Nano,
Journal Year:
2023,
Volume and Issue:
18(1), P. 119 - 135
Published: Dec. 20, 2023
T
cell-mediated
immunity
plays
a
pivotal
role
in
cancer
immunotherapy.
The
anticancer
actions
of
cells
are
coordinated
by
sequence
biological
processes,
including
the
capture
and
presentation
antigens
antigen-presenting
(APCs),
activation
APCs,
subsequent
killing
activated
cells.
However,
have
various
means
to
evade
immune
responses.
Meanwhile,
these
vulnerabilities
provide
potential
targets
for
treatments.
Functional
nucleic
acids
(FNAs)
make
up
class
synthetic
with
specific
functions.
With
their
diverse
functionality,
good
biocompatibility,
high
programmability,
FNAs
attracted
widespread
interest
This
Review
focuses
on
recent
research
progress
employing
as
molecular
tools
immunotherapy,
corresponding
challenges
prospects.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: May 1, 2024
The
metal-nucleic
acid
nanocomposites,
first
termed
frameworks
(MNFs)
in
this
work,
show
extraordinary
potential
as
functional
nanomaterials.
However,
thus
far,
realized
MNFs
face
limitations
including
harsh
synthesis
conditions,
instability,
and
non-targeting.
Herein,
we
discover
that
longer
oligonucleotides
can
enhance
the
efficiency
stability
of
by
increasing
oligonucleotide
folding
entanglement
probabilities
during
reaction.
Besides,
provide
upgraded
metal
ions
binding
facilitating
to
load
macromolecular
protein
drugs
at
room
temperature.
Furthermore,
facilitate
expansion
nucleotide
sequences,
enabling
disease-targeted
MNFs.
As
a
proof-of-concept,
build
an
interferon
regulatory
factor-1(IRF-1)
loaded
Ca
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(3), P. 1268 - 1274
Published: Jan. 9, 2024
RNA-cleaving
DNAzymes
have
emerged
as
a
promising
tool
for
metal
ion
detection.
Achieving
spatiotemporal
control
over
their
catalytic
activity
is
essential
understanding
the
role
of
ions
in
various
biological
processes.
While
photochemical
and
endogenous
stimuli-responsive
approaches
shown
potential
controlled
imaging
using
DNAzymes,
limitations
such
photocytotoxicity,
poor
tissue
penetration,
or
off-target
activation
hindered
application
safe
precise
detection
vivo.
We
herein
report
chemically
inducible
DNAzyme
which
core
modified
to
contain
chemical
caging
groups
at
selected
backbone
sites
through
systematic
screening.
This
exhibits
minimal
leakage
can
be
reactivated
by
small
molecule
selenocysteines,
effectively
remove
restore
DNAzyme.
Benefiting
from
these
findings,
we
designed
fluorogenic
sensor
with
tunable
high
selectivity
live
cells
design
expands
toolbox
controlling
easily
adapted
detect
other
vivo
changing
module,
offering
opportunities
biomedical
diagnosis.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(18)
Published: March 22, 2024
Abstract
DNAzymes
exhibit
tremendous
application
potentials
in
the
field
of
biosensing
and
gene
regulation
due
to
its
unique
catalytic
function.
However,
spatiotemporally
controlled
DNAzyme
activity
remains
a
daunting
challenge,
which
may
cause
nonspecific
signal
leakage
or
silencing
systems.
Here,
we
report
photochemical
approach
via
modular
weaving
active
into
skeleton
tetrahedral
DNA
nanocages
(TDN)
for
light‐triggered
on‐demand
liberation
thus
conditional
control
activity.
We
demonstrate
that
direct
encoding
TDN
could
improve
biostability
ensure
delivery
efficiency,
comparing
with
conventional
surface
anchoring
strategy.
Furthermore,
molecular
nanostructures
allows
remote
DNAzyme‐mediated
high
spatiotemporal
precision
light.
In
addition,
is
applicable
editing
functions
other
functional
nucleic
acids.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(24), P. 9866 - 9875
Published: June 5, 2024
Herein,
a
dual
self-protected
DNAzyme-based
3D
DNA
walker
(dSPD
walker),
composed
of
activated
walking
particles
(ac-dSPWPs)
and
track
(TPs),
was
constructed
for
ultrasensitive
ultrahigh-speed
fluorescence
detection
imaging
microRNAs
(miRNAs)
in
living
cells.
Impressively,
compared
with
the
defect
that
"one"
target
miRNA
only
initiates
arm
conventional
single
DNAzyme
walker,
dSPD
benefits
from
secondary
amplification
spatial
confinement
effect
could
guide
to
generate
"n"
targets,
thereby
initiating
nearby
strands
immediately,
realizing
initial
rate
over
one-magnitude-order
faster
than
one.
Moreover,
process
relative
motion
between
ac-dSPWPs
TPs,
cleave
multiple
substrate
simultaneously
speed
up
movement
reduce
derailment
rate,
as
well
combine
successive
TPs
facilitate
large
amount
continuous
signal
accumulation,
achieving
an
ultrafast
miRNA-221
within
10
min
vitro
high
sensitivity
low
limit
0.84
pM.
In
addition,
nanospheres
obtained
by
rolling
circle
reaction
can
capture
Cy5
dispersed
liquids,
which
achieves
high-contrast
miRNA-221,
resulting
further
cancer
The
proposed
strategy
has
made
bold
innovation
rapid
sensitive
intracellular
low-abundance
biomarkers,
offering
promising
application
early
diagnosis
relevant
research
tumors.
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(22), P. 28041 - 28055
Published: May 20, 2024
Bacterial
infection
poses
a
significant
challenge
to
wound
healing
and
skin
regeneration,
leading
substantial
economic
burdens
on
patients
society.
Therefore,
it
is
crucial
promptly
explore
develop
effective
methodologies
for
bacterial
infections.
Herein,
we
propose
novel
approach
synthesizing
nanostructures
based
antisense
oligonucleotides
(ASOs)
through
the
coordination-driven
self-assembly
of
Zn2+
with
ASO
molecules.
This
aims
provide
synergistic
therapy
chronic
infections
caused
by
Staphylococcus
aureus
(S.
aureus).
The
resulting
hybrid
nanoparticles
successfully
preserve
structural
integrity
biological
functionalities
ASOs,
demonstrating
excellent
encapsulation
efficiency
bioaccessibility.
In
vitro
antibacterial
experiments
reveal
that
Zn-ASO
NPs
exhibit
antimicrobial
properties
against
Escherichia
coli,
aureus,
Bacillus
subtilis.
ability
attributed
high
concentration
metal
zinc
ions
generation
levels
reactive
oxygen
species.
Additionally,
ftsZ-ASO
effectively
inhibits
expression
ftsZ
gene,
further
enhancing
effect.
vivo
assays
demonstrate
promote
optimal
favorable
biocompatibility
S.
infections,
in
residual
infected
area
less
than
8%.
combined
strategy,
which
integrates
gene
metal-coordination-directed
self-assembly,
not
only
achieves
augmented
outcomes
but
also
expands
horizons
coordination
chemistry.
Moreover,
addresses
gap
application
metal-coordination
thereby
advancing
field
ASO-based
therapeutic
approaches.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(23), P. 15815 - 15824
Published: June 4, 2024
Ribonuclease
targeting
chimera
(RIBOTAC)
represents
an
emerging
strategy
for
targeted
therapy.
However,
RIBOTAC
that
is
selectively
activated
by
bio-orthogonal
or
cell-specific
triggers
has
not
been
explored.
We
developed
a
of
inducible
(iRIBOTAC)
enables
on-demand
degradation
G-quadruplex
(G4)
RNAs
precision
cancer
iRIBOTAC
designed
coupling
RNA
G4
binder
with
caged
ribonuclease
recruiter,
which
can
be
decaged
reaction,
tumor-specific
enzyme,
metabolite.
A
bivalent
engineered
conjugating
near-infrared
(NIR)
fluorescence
ligand
to
noncompetitive
ligand,
conferring
activation
on
binding
G4s
synergistically
enhanced
affinity.
demonstrated
greatly
knockdown
upon
under
stimulus,
dysregulation
gene
expressions
involving
cell
killing,
channel
regulator
activity,
and
metabolism
as
revealed
sequencing.
This
also
shows
crucial
effect
fate
remarkable
biochemical
hallmarks
apoptosis.
Mice
model
studies
demonstrate
allows
selective
imaging
growth
suppression
tumors
controls,
highlighting
silencing
valuable
paradigm
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(21), P. 8682 - 8688
Published: May 17, 2024
Programming
ultrasensitive
and
stimuli-responsive
DNAzyme-based
probes
holds
great
potential
for
on-demand
biomarker
detection.
Here,
an
optically
triggered
DNAzyme
platform
was
reported
activation-sensitive
electrochemiluminescence
(ECL)
c-myc
mRNA
analysis.
In
this
design,
the
sensing
recognition
function
of
split
(SDz)
probe
silent
by
engineering
a
blocking
sequence
containing
photocleavable
linker
(PC-linker)
group
at
defined
site
that
could
be
indirectly
cleaved
302
nm
ultraviolet
(UV)
light.
When
SDz
were
assembled
on
Au
nanoparticles
potassium
(K)
element
doped
graphitic
carbon
nitride
nanosheet
(K-doped
g-C3N4)
covered
electrode,
UV
light
activation
induces
configurational
switching
consequently
formation
active
with
help
target
mRNA,
allowing
cleavage
substrate
strand
magnesium
ions
(Mg2+).
Thus,
release
ferrocene
(Fc)-labeled
2
contributed
to
extreme
ECL
signal
recovery.
meantime,
released
combined
another
inactive
motif
form
repeat
cyclic
reaction,
resulting
in
amplification.
Furthermore,
according
responses
toward
two
other
designed
nPC-SDz
m-SDz
probes,
we
demonstrated
controlled
mediated
photoactivation
biosensor
"on
demand"
effectively
constrained
interest.
Moreover,
false
positive
signals
also
avoided
due
such
design
Therefore,
study
provided
simple
methodology
may
broadly
applicable
investigating
mRNA-associated
physiological
events
difficult
access
using
traditional
probes.
Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
136(18)
Published: March 22, 2024
Abstract
DNAzymes
exhibit
tremendous
application
potentials
in
the
field
of
biosensing
and
gene
regulation
due
to
its
unique
catalytic
function.
However,
spatiotemporally
controlled
DNAzyme
activity
remains
a
daunting
challenge,
which
may
cause
nonspecific
signal
leakage
or
silencing
systems.
Here,
we
report
photochemical
approach
via
modular
weaving
active
into
skeleton
tetrahedral
DNA
nanocages
(TDN)
for
light‐triggered
on‐demand
liberation
thus
conditional
control
activity.
We
demonstrate
that
direct
encoding
TDN
could
improve
biostability
ensure
delivery
efficiency,
comparing
with
conventional
surface
anchoring
strategy.
Furthermore,
molecular
nanostructures
allows
remote
DNAzyme‐mediated
high
spatiotemporal
precision
light.
In
addition,
is
applicable
editing
functions
other
functional
nucleic
acids.
