Tandem‐Controlled Dynamic DNA Assembly Enables Temporally‐Selective Orthogonal Regulation of cGAS–STING Stimulation DOI Open Access
Xiang‐Fei Li, Fangzhi Yu, Lele Li

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstract Despite advances in the controlled reconfiguration of DNA structures for biological applications, dearth strategies that allow orthogonal regulation immune pathways remains a challenge. Here, we report first time an endogenous and exogenous tandem‐regulated assembly strategy enables orthogonally stimulation cyclic GMP‐AMP synthase (cGAS)‐stimulator interferon genes (STING) pathway. A motif containing two palindromic sequences is engineered with abasic site (AP)‐connected blocking sequence to inhibit its self‐assembly function, while apurinic/apyrimidinic endonuclease 1 (APE1)‐triggered enzymatic cleavage AP into long double‐stranded structures, thus realizing allosteric activation catalytic activity cGAS produce 2’3’‐cyclic‐GMP‐AMP STING stimulation. Importantly, demonstrate APE1‐regulated allows cell‐selective cGAS–STING signaling. Furthermore, by re‐engineering photocleavable group, enzyme‐triggered operate (switched “ON”), whereas light‐mediated fragmentation termination such “OFF”), thereby achieving control over regulation. This work highlights tandem regulated modulate pathway manner.

Language: Английский

Chemically modified DNAzymes-based electrochemical sensor for binary and highly sensitive detection of reactive oxygen species DOI Creative Commons
Baoting Dou, Hui Shen, Zhimin Li

et al.

Chemical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

A chemically modified DNAzyme-based electrochemical sensor was developed for binary and highly sensitive detection of reactive oxygen species.

Language: Английский

Citations

2
Phototherapy: progress, challenges, and opportunities DOI
Xi Yuan,

Junliang Zhou,

Lin Yuan

et al.

Science China Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

Language: Английский

Citations

14
DNA Nanotechnology Targeting Mitochondria: From Subcellular Molecular Imaging to Tailor‐Made Therapeutics DOI
Fangzhi Yu, Xiang‐Fei Li,

Chuangui Sheng

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(36)

Published: June 14, 2024

Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio-applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria-targeted tumor treatment. We first highlight engineering mitochondria-localized nanosensors situ detection diverse key molecules are essential to maintain mitochondrial functions, including microRNA, enzymes, small molecules, metal ions. Then, compile developments anti-tumor therapy, modularly designed nanodevices delivery therapeutic agents, programmed assembly interference. will place emphasis clarification chemical principles how nanobiotechnology can be various Finally, remaining challenges future directions field discussed, hoping inspire further development advanced toolkits both academic clinical regarding mitochondria.

Language: Английский

Citations

12
Recent advances on DNAzyme for bioimaging, biosensing and cancer therapy DOI
Pei Sun,

Hongquan Gou,

Xinran Che

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(78), P. 10805 - 10821

Published: Jan. 1, 2024

DNAzymes, a class of single-stranded catalytic DNA with good stability, high activity, and easy synthesis, functionalization modification properties, have garnered significant interest in the realm biosensing bioimaging. Their integration fluorescent dyes or chemiluminescent moieties has led to remarkable bioimaging outcomes, while DNAzyme-based biosensors demonstrated robust sensitivity selectivity detecting metal ions, nucleic acids, proteins, enzyme activities, exosomes, bacteria microorganisms. In addition, by delivering DNAzymes into tumor cells, mRNA therein can be cleaved regulate expression corresponding which further propelled application cancer gene therapy synergistic therapy. This paper reviews strategies for screening attractive such as SELEX high-throughput sequencing, briefly describes amplification mainly include hairpin assembly (CHA), walker, hybridization chain reaction (HCR), origami, CRISPR-Cas12a, rolling circle (RCA), aptamers. applications bioimaging, biosensing, are also highlighted. Subsequently, possible challenges these practical pointed out, future research directions suggested.

Language: Английский

Citations

5
Supramolecular DNA Nanodevice Assembled via RCA and HCR Cascade Reaction on Tetrahedral DNA Nanostructure for Sensitive Detection and Intracellular Imaging of Dual-miRNAs Associated with Liver Cancer DOI

Xue-Mei Zhou,

Si–Yi Chen, Yaqin Chai

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Herein, a supramolecular DNA nanodevice was formed via the rolling circle amplification (RCA) and hybridization chain reaction (HCR) cascade on tetrahedral nanostructure (TDN) to achieve simultaneous sensitive detection intracellular imaging of dual-miRNAs related liver cancer. The effectively addressed limitations low probe loading capacity in traditional TDN nanodevices by enriching plenty signal probes around single TDN, significantly enhancing fluorescence signal. Impressively, with fulcrum dense structure imparted strong rigidity, ensuring stability decrease aggregation quenching for further increasing response. Consequently, biosensor based enabled miRNA221 miRNA222, achieved accurate situ displaying improved capabilities compared TDN-based nanodevices. More importantly, precise miRNA222 could distinguish hepatocellular carcinoma cells different degrees metastasis from human normal cells, providing more information diagnosis development carcinoma.

Language: Английский

Citations

0
Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary DOI
Lei Wang, Tingting Zhao,

Congkai Wang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Developing tumor cell-specific imaging approaches is essential for the clear delineation of margins. However, traditional suffered from low reaction kinetics as well limited specificity resulting their "always active" sensing mode, making it difficult to accurately depict boundary. To address these limitations, we developed an endogenous enzyme-activated spatial confinement DNA nanowire probe (E-SCNW) with enhanced tumor/normal cell discrimination ratio high precision cells The effect can improve kinetics, and enzyme-activation design confine fluorescence response region. Additionally, no additional delivery carriers were required during cross membrane into intracellular space. It worth noting that benefiting design, detection limit was decreased by nearly 25.6-fold 4.46-fold through using E-SCNW, indicating promising prospects in high-precision

Language: Английский

Citations

0
Advancements in DNAzyme-based biosensors for the detection of hazardous substances in foodstuff: current applications and future perspectives DOI

Taolin Li,

Yijia Zhang, Weiwei Cheng

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 20

Published: April 6, 2025

DNAzyme-based biosensors have emerged as a promising tool for ensuring food safety due to their high sensitivity, specificity, and potential rapid, cost-effective detection of hazardous substances. These leverage DNAzymes-catalytically active DNA molecules-to detect range contaminants, including metal ions, fungal toxins, pesticides, pathogens. While show significant advantages over conventional techniques, challenges such nuclease degradation, interference from complex sample matrices, the costs associated with DNAzyme synthesis still hinder widespread application. Recent advancements in stability DNAzymes, immobilization strategies, integration nanomaterials are progressively addressing these limitations, enhancing performance reliability sensors. This review highlights structural catalytic characteristics assesses current applications safety, discusses innovative strategies overcome existing challenges. The continuous evolution biosensors, particularly design device integration, holds great promise future role routine, reliable analysis.

Language: Английский

Citations

0
DNA Nanotechnology Targeting Mitochondria: From Subcellular Molecular Imaging to Tailor‐Made Therapeutics DOI
Fangzhi Yu, Xiang‐Fei Li,

Chuangui Sheng

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(36)

Published: June 14, 2024

Abstract Mitochondria, one of the most important organelles, represent a crucial subcellular target for fundamental research and biomedical applications. Despite significant advances in design DNA nanotechnologies variety bio‐applications, dearth strategies that enable mitochondria targeting molecular imaging therapy remains an outstanding challenge this field. In Minireview, we summarize recent progresses on emerging application nanotechnology mitochondria‐targeted tumor treatment. We first highlight engineering mitochondria‐localized nanosensors situ detection diverse key molecules are essential to maintain mitochondrial functions, including microRNA, enzymes, small molecules, metal ions. Then, compile developments anti‐tumor therapy, modularly designed nanodevices delivery therapeutic agents, programmed assembly interference. will place emphasis clarification chemical principles how nanobiotechnology can be various Finally, remaining challenges future directions field discussed, hoping inspire further development advanced toolkits both academic clinical regarding mitochondria.

Language: Английский

Citations

3
DNA Nanocage-Assisted Size-Selective Recognition and Quantification toward Low-Mass Soluble β-Amyloid Oligomers DOI
Jia Chen, Qi Liu,

Yongchun Fu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11397 - 11403

Published: June 28, 2024

Low-mass soluble β-amyloid peptide oligomers (LSAβOs) play a crucial role in the pathogenesis of Alzheimer's disease. However, these exhibit heterogeneity terms structure, stability, and stoichiometry, their abundance biofluids is low, making accurate identification challenging. In this study, we developed DNA nanocage-assisted method for selective sizing sensitive quantification LSAβOs serum. Using LSAβO less than 10 kDa (LSAβO10kD) 30 (LSAβO30kD) as models, size-matching rules between nanocages were investigated, two appropriate selected detection LSAβOs, respectively. Both functionalized by encapsulating oligomer's aptamer complementary sequence within cavities. Once entered corresponding nanocage cavity, was released, triggering hybridization chain reaction on an electrochemical sensing platform. The system achieved size-selective discrimination LSAβO10kD with linear range 10–150 pM LSAβO30kD 15–150 pM. Real sample testing confirmed applicability blood-based diagnosis. analysis platform provides accurate, highly selective, approach oligomer analysis, which significant amyloid protein research related disease

Language: Английский

Citations

2
A DNAzyme‐Based Nanohybrid for Ultrasound and Enzyme Dual‐Controlled mRNA Regulation and Combined Tumor Treatment DOI Open Access
Hanji Huang, Yaoxuan Chen, Lele Li

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract Despite the significant potential of RNA‐cleaving DNAzymes for gene regulation, their application is limited by low therapeutic efficacy and lack cell‐specific control. Here, a DNAzyme‐based nanohybrid designed ultrasound (US)‐controlled, enzyme‐activatable mRNA regulation presented, enabling tumor cell‐selective combination therapy. The constructed coordination‐directed self‐assembly an enzymatically‐triggerable DNAzyme (En‐Dz) natural sonosensitizer hemoglobin (Hb). Controlled US exposure induces reactive oxygen species generation from Hb units, which not only facilitates efficient endosomal escape En‐Dz, but also promotes translocation specific enzyme nucleus to cytoplasm, thereby enhancing efficacy. Notably, enzyme‐triggered, spatiotemporally‐controlled activation En‐Dz's catalytic activity results in enhanced cancer‐cell selectivity treatment. Furthermore, enzyme‐activated sonodynamic therapy significantly enhances anti‐tumor both vitro vivo. This work highlights integrating strategy overcome current limitations regulators, providing approach precise

Language: Английский

Citations

2