Integration of optogenetics with complementary methodologies in systems neuroscience

Nature reviews. Neuroscience, Journal Year: 2017, Volume and Issue: 18(4), P. 222 - 235

Published: March 17, 2017

Language: Английский

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Genetically encoded indicators of neuronal activity

Nature Neuroscience, Journal Year: 2016, Volume and Issue: 19(9), P. 1142 - 1153

Published: Aug. 26, 2016

Language: Английский

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The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

Trends in Biochemical Sciences, Journal Year: 2016, Volume and Issue: 42(2), P. 111 - 129

Published: Nov. 1, 2016

Language: Английский

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High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor

Science, Journal Year: 2015, Volume and Issue: 350(6266), P. 1361 - 1366

Published: Dec. 11, 2015

In vivo imaging of neuronal voltage spikes Neuroscientists have long sought tools that allow optical individual neurons' membrane dynamics in awake behaving animals. Gong et al. genetically engineered a protein indicator can report action potentials with <1-ms precision and orders magnitude lower spike detection error rates than were previously possible. They thus able to record the brains mice fruit flies. Science , this issue p. 1361

Language: Английский

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Labeling of active neural circuits in vivo with designed calcium integrators

Science, Journal Year: 2015, Volume and Issue: 347(6223), P. 755 - 760

Published: Feb. 12, 2015

The identification of active neurons and circuits in vivo is a fundamental challenge understanding the neural basis behavior. Genetically encoded calcium (Ca(2+)) indicators (GECIs) enable quantitative monitoring cellular-resolution activity during However, such require online within limited field view. Alternatively, post hoc staining immediate early genes (IEGs) indicates highly cells entire brain, albeit with poor temporal resolution. We designed fluorescent sensor, CaMPARI, that combines genetic targetability link to GECIs permanent, large-scale labeling IEGs, allowing temporally precise "activity snapshot" large tissue volume. CaMPARI undergoes efficient irreversible green-to-red conversion only when elevated intracellular Ca(2+) experimenter-controlled illumination coincide. demonstrate utility freely moving larvae zebrafish flies, head-fixed mice adult flies.

Language: Английский

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Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

Chemical Reviews, Journal Year: 2018, Volume and Issue: 118(24), P. 11707 - 11794

Published: Dec. 14, 2018

Cellular signaling networks are the foundation which determines fate and function of cells as they respond to various cues stimuli. The discovery fluorescent proteins over 25 years ago enabled development a diverse array genetically encodable biosensors that capable measuring spatiotemporal dynamics signal transduction pathways in live cells. In an effort encapsulate breadth have expanded, we endeavored assemble comprehensive list published engineered biosensors, discuss many molecular designs utilized their development. Then, review how high temporal spatial resolution afforded by has aided our understanding regulation at cellular subcellular level. Finally, highlight some emerging areas research both biosensor design applications on forefront

Language: Английский

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Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

Science, Journal Year: 2019, Volume and Issue: 365(6454), P. 699 - 704

Published: Aug. 1, 2019

Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility existing GEVIs has been limited by brightness photostability fluorescent proteins rhodopsins. We engineered a GEVI, called Voltron, that uses bright photostable synthetic dyes instead protein-based fluorophores, thereby extending number neurons imaged simultaneously in vivo factor 10 enabling imaging for significantly longer durations relative to GEVIs. used Voltron mice, zebrafish, fruit flies. In mouse cortex, allowed single-trial recording spikes subthreshold signals from dozens over 15-minute period continuous imaging. larval enabled precise correlation spike timing with behavior.

Language: Английский

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Next-generation probes, particles, and proteins for neural interfacing

Science Advances, Journal Year: 2017, Volume and Issue: 3(6)

Published: June 2, 2017

Multimodal and multidisciplinary approaches lead to next-generation technologies for reading modulating neural function.

Language: Английский

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A Guide to Fluorescent Protein FRET Pairs

Sensors, Journal Year: 2016, Volume and Issue: 16(9), P. 1488 - 1488

Published: Sept. 14, 2016

Förster or fluorescence resonance energy transfer (FRET) technology and genetically encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live cells with high spatiotemporal resolution. Fluorescent proteins (FPs) are most commonly used as both donor acceptor fluorophores biosensors, especially since FPs encodable live-cell compatible. In this review, we will an overview of methods to measure changes biological contexts, discuss the palette FP pairs developed their relative strengths weaknesses, note important factors consider when using studies.

Language: Английский

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Next-generation GRAB sensors for monitoring dopaminergic activity in vivo

Nature Methods, Journal Year: 2020, Volume and Issue: 17(11), P. 1156 - 1166

Published: Oct. 21, 2020

Language: Английский

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