Metabolomic profiles in serum and urine uncover novel biomarkers in children with nephrotic syndrome DOI
Lidan Hu, Li Lin, Guoping Huang

et al.

European Journal of Clinical Investigation, Journal Year: 2023, Volume and Issue: 53(7)

Published: March 1, 2023

Nephrotic syndrome is common in children and adults worldwide, steroid-sensitive nephrotic (SSNS) accounts for 80%. Aberrant metabolism involvement early SSNS sparsely studied, its pathogenesis remains unclear. Therefore, the goal of this study was to investigate changes initiated patients-related metabolites through serum urine metabolomics discover novel potential metabolic pathways.Serum samples (27 56 controls) (17 24 were collected. Meanwhile, non-targeted analyses performed by ultra-high-performance liquid chromatography-quadrupole time flight-mass spectrometry (UHPLC-QTOF-MS) determine SSNS. We applied causal inference model, DoWhy assess effects several selected metabolites. An ultraperformance chromatography-tandem mass (UPLC-MS/MS) used validate hits (D-mannitol, dulcitol, D-sorbitol, XMP, NADPH, NAD, bilirubin, α-KG-like) 41 43 controls. In addition, pathways explored.Compared urine, analysis more clearly discriminated at 194 differential five obtained group. Eight identified establishing diagnostic model SSNS, four variables had a positive effect. After validation targeted MS, except others have similar trends like untargeted analysis.With further quantitative analysis, we found seven may be new biomarkers risk prediction diagnosis

Language: Английский

TGF-β as A Master Regulator of Aging-Associated Tissue Fibrosis DOI Creative Commons
Lili Ren, Hua Miao, Yanni Wang

et al.

Aging and Disease, Journal Year: 2023, Volume and Issue: 14(5), P. 1633 - 1633

Published: Jan. 1, 2023

Fibrosis is the abnormal accumulation of extracellular matrix proteins such as collagen and fibronectin. Aging, injury, infections, inflammation can cause different types tissue fibrosis. Numerous clinical investigations have shown a correlation between degree liver pulmonary fibrosis in patients telomere length mitochondrial DNA content, both which are signs aging. Aging involves gradual loss function over time, results homeostasis and, ultimately, an organism's fitness. A major feature aging senescent cells. Senescent cells abnormally continuously accumulate late stages life, contributing to age-related deterioration, among other characteristics. Furthermore, generates chronic inflammation, decreases organ function. This finding suggests that closely related. The transforming growth factor-beta (TGF-β) superfamily plays crucial role physiological pathological processes aging, immune regulation, atherosclerosis, In this review, functions TGF-β normal organs, fibrotic tissues discussed: signalling altered with age indicator pathology associated addition, review discusses potential targeting noncoding.

Language: Английский

Citations

45
Lactobacillus species ameliorate membranous nephropathy through inhibiting the aryl hydrocarbon receptor pathway via tryptophan‐produced indole metabolites DOI
Hua Miao, Yanni Wang,

Xiao‐Yong Yu

et al.

British Journal of Pharmacology, Journal Year: 2023, Volume and Issue: 181(1), P. 162 - 179

Published: Aug. 19, 2023

Abstract Background and Purpose Membranous nephropathy (MN) is an immune‐mediated glomerular disease in adults. Antibody‐ antigen‐bonding mechanisms have been largely clarified, but the subepithelium immune complex deposition‐mediated downstream molecular are currently unresolved. Increasing evidence has suggested that gut microbiota contribute to MN pathogenesis. Experimental Approach In this study, we identified alterations faecal serum metabolites mediate aryl hydrocarbon receptor (AhR) mechanism cationic bovine albumin (CBSA)‐induced rats patients with idiopathic (IMN). Key Results Impaired renal function correlated relative abundance of reduced probiotics, Lactobacillus Bifidobacterium , altered levels tryptophan‐produced indole derivatives (TPIDs) rats. Further results showed five namely johnsonii murinus vaginalis reuteri animalis positively decreased indole‐3‐pyruvic acid, indole‐3‐aldehyde tryptamine negatively increased indole‐3‐lactic acid indole‐3‐acetic Altered probiotics TPIDs also were observed IMN. studies exhibited a significant increase intrarenal mRNA expression AhR its target genes CYP1A1 CYP1A2 CYP1B1 which accompanied by protein down‐regulated cytoplasmic AhR, up‐regulated nuclear IMN patients. Conclusion Implications Activation signalling pathway may involve TPIDs. These data suggest could influence through engage host receptors.

Language: Английский

Citations

39
Atractylodes macrocephala Koidz polysaccharide improves glycolipid metabolism disorders through activation of aryl hydrocarbon receptor by gut flora-produced tryptophan metabolites DOI
Ziwen He, Jing-yan Guo, Huiwen Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 126987 - 126987

Published: Sept. 18, 2023

Language: Английский

Citations

27
The nuclear factor kappa B signaling pathway is a master regulator of renal fibrosis DOI Creative Commons
Na Ren, Wenfeng Wang, Liang Zou

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 16, 2024

Renal fibrosis is increasingly recognized as a global public health problem. Acute kidney injury (AKI) and chronic disease (CKD) both result in renal fibrosis. Oxidative stress inflammation play central roles progressive are closely linked form vicious cycle which oxidative induces through various molecular mechanisms. Ample evidence has indicated that hyperactive nuclear factor kappa B (NF-ƙB) signaling pathway plays pivotal role Hyperactive NF-ƙB causes the activation recruitment of immune cells. Inflammation, turn, triggers production reactive oxygen species nitrogen by activating leukocytes resident These events mediate organ apoptosis, necrosis, Therefore, developing strategy to target important for effective treatment This Review summarizes effect on context AKI CKD (immunoglobulin A nephropathy, membranous diabetic hypertensive transplantation). Therapies targeting pathway, including natural products, also discussed. In addition, NF-ƙB-dependent non-coding RNAs involved crucial targets development treatments disease. provides clear pathophysiological rationale specific concept-driven therapeutic pathway.

Language: Английский

Citations

12
Understanding the physiological mechanisms and therapeutic targets of diseases: Lipidomics strategies DOI
Qiang Yang, Ying Cai,

Zhibo Wang

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: 363, P. 123411 - 123411

Published: Jan. 21, 2025

Language: Английский

Citations

1
Luteolin alleviates depression‐like behavior by modulating glycerophospholipid metabolism in the hippocampus and prefrontal cortex of LOD rats DOI Creative Commons
Xiaofeng Wu, Hanfang Xu, Ning-Xi Zeng

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2023, Volume and Issue: 30(3)

Published: Sept. 16, 2023

Abstract Background Late‐onset depression (LOD) is defined as primary that first manifests after the age of 65. Luteolin (LUT) a natural flavonoid has shown promising antidepressant effects and improvement in neurological function previous studies. Aims In this study, we utilized UPLC–MS/MS non‐targeted metabolomics techniques, along with molecular docking technology experimental validation, to explore mechanism LUT treating LOD from perspective. Results The behavioral results our study demonstrate significantly ameliorated anxiety depression‐like behaviors while enhancing cognitive rats. Metabolomic analysis revealed on rats were primarily mediated through glycerophospholipid metabolic pathway hippocampus prefrontal cortex. levels key lipid metabolites, phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE), altered by treatment, PC PE showing significant correlations indices. Molecular indicated had strong binding activity synthase 1 (PTDSS1), 2 (PTDSS2), decarboxylase (PISD), which are involved transformation synthesis PC, PE, PS. Lastly, explored reasons for opposing trends PS cortex perspective autophagy, may be attributable bidirectional regulation autophagy distinct brain regions. Conclusions Our alterations metabolism pathways both Moreover, appears regulate disorders specifically modulating different regions rats, consequently alleviating behavior these animals.

Language: Английский

Citations

19
Arachidonic acid metabolism as a therapeutic target in AKI-to-CKD transition DOI Creative Commons
Xiaojun Li,

Ping Suo,

Yanni Wang

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: March 8, 2024

Arachidonic acid (AA) is a main component of cell membrane lipids. AA mainly metabolized by three enzymes: cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP450). Esterified hydrolysed phospholipase A 2 into free form that further COX, LOX CYP450 to wide range bioactive mediators, including prostaglandins, lipoxins, thromboxanes, leukotrienes, hydroxyeicosatetraenoic acids epoxyeicosatrienoic acids. Increased mitochondrial oxidative stress considered be central mechanism in the pathophysiology kidney. Along with increased stress, apoptosis, inflammation tissue fibrosis drive progressive loss kidney function, affecting glomerular filtration barrier tubulointerstitium. Recent studies have shown its active derivative eicosanoids play important roles regulation physiological function pathogenesis disease. These factors are potentially novel biomarkers, especially context their involvement inflammatory processes stress. In this review, we introduce metabolic pathways discuss molecular mechanisms which these affect progression acute injury (AKI), diabetic nephropathy (DN) renal carcinoma (RCC). This review may provide new therapeutic targets for identification AKI CKD continuum.

Language: Английский

Citations

8
Traditional Chinese medicine improved diabetic kidney disease through targeting gut microbiota DOI Creative Commons
Xiaqing Wu, Lei Zhao,

Yanlong Zhao

et al.

Pharmaceutical Biology, Journal Year: 2024, Volume and Issue: 62(1), P. 423 - 435

Published: May 17, 2024

Context Diabetic kidney disease (DKD) affects nearly 40% of diabetic patients, often leading to end-stage renal that requires replacement therapies, such as dialysis and transplantation. The gut microbiota, an integral aspect human evolution, plays a crucial role in this condition. Traditional Chinese medicine (TCM) has shown promising outcomes ameliorating DKD by addressing the microbiota.

Language: Английский

Citations

8
Bile acid metabolism in health and ageing-related diseases DOI
Xiaojun Li,

Chu Fang,

Ruihua Zhao

et al.

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: 225, P. 116313 - 116313

Published: May 22, 2024

Language: Английский

Citations

7
Poria cocos: traditional uses, triterpenoid components and their renoprotective pharmacology DOI

Zhi-yuan Guo,

X. Wu,

Shui-juan Zhang

et al.

Acta Pharmacologica Sinica, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

Language: Английский

Citations

7