Two sex pheromone receptors for sexual communication in the American cockroach

Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(7), P. 1455 - 1467

Published: March 22, 2024

Language: Английский

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Insulin/IGF signaling and TORC1 promote vitellogenesis via inducing juvenile hormone biosynthesis in the American cockroach

Development, Journal Year: 2020, Volume and Issue: 147(20)

Published: Oct. 15, 2020

ABSTRACT Vitellogenesis, including vitellogenin (Vg) production in the fat body and Vg uptake by maturing oocytes, is of great importance for successful reproduction adult females. The endocrinal nutritional regulation vitellogenesis differs distinctly insects. Here, complex crosstalk between juvenile hormone (JH) two nutrient sensors insulin/IGF signaling (IIS) target rapamycin complex1 (TORC1), was investigated to elucidate molecular mechanisms American cockroach, Periplaneta americana. Our data showed that a block JH biosynthesis or action arrested vitellogenesis, part inhibiting expression doublesex (Dsx), key transcription factor gene involved sex determination cascade. Depletion IIS TORC1 blocked both vitellogenesis. Importantly, analog methoprene, but not bovine insulin (to restore IIS) amino acids activity), restored neck-ligated (IIS-, TORC1- JH-deficient) rapamycin-treated (TORC1- cockroaches. Combining classic physiology with modern techniques, we have demonstrated promote mainly via inducing cockroach.

Language: Английский

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Field cricket genome reveals the footprint of recent, abrupt adaptation in the wild

Evolution Letters, Journal Year: 2019, Volume and Issue: 4(1), P. 19 - 33

Published: Dec. 20, 2019

Abstract Evolutionary adaptation is generally thought to occur through incremental mutational steps, but large leaps can during its early stages. These are challenging study in nature due the difficulty of observing new genetic variants as they arise and spread, characterizing their genomic dynamics important for understanding factors favoring rapid adaptation. Here, we report consequences recent, adaptive song loss a Hawaiian population field crickets (Teleogryllus oceanicus). A discrete variant, flatwing, appeared spread approximately 15 years ago. Flatwing erases sound-producing veins on male wings. silent flatwing males protected from lethal, eavesdropping parasitoid fly. We sequenced, assembled annotated cricket genome, produced linkage map, identified quantitative trait locus covering region X chromosome. Gene expression profiling showed that associated with extensive genome-wide effects embryonic gene expression. found express feminized chemical pheromones. This feminizing effect, different sexual signaling modality, genetically genotype. Our findings suggest stages evolutionary extreme pressures be accompanied by greater phenotypic disruption than previously appreciated, highlight how abrupt might involve suites traits pleiotropy or hitchhiking.

Language: Английский

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Genomics- and Peptidomics-Based Discovery of Conserved and Novel Neuropeptides in the American Cockroach

Journal of Proteome Research, Journal Year: 2020, Volume and Issue: 20(2), P. 1217 - 1228

Published: Nov. 9, 2020

As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, of great interest in both basic applied research. Previous studies on P. americana neuropeptide identification have been based biochemical isolation molecular cloning. In present study, integrated approach genomics- peptidomics-based discovery was performed for this species. First, 67 conserved or neurohormone precursor genes were predicted via silico analysis genome transcriptome. Using large-scale peptidomic peptide extracts from four different tissues (the central nervous system, corpora cardiac allata complex, midgut, male accessory gland), 35 (predicted) neuropeptides potential (novel) then identified. Subsequent experiments revealed tissue distribution, sex difference, developmental patterns two (allatostatin B short F) novel (PaOGS36577). Our study shows comprehensive neuropeptidome detailed spatiotemporal distribution patterns, providing solid basis future functional cockroach (data are available ProteomeXchange with identifier PXD021660).

Language: Английский

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A chromosome‐level genome of the mud crab (Scylla paramamosain estampador) provides insights into the evolution of chemical and light perception in this crustacean

Molecular Ecology Resources, Journal Year: 2021, Volume and Issue: 21(4), P. 1299 - 1317

Published: Jan. 19, 2021

Abstract Mud crabs, found throughout the Indo‐Pacific region, are coastal species that important fisheries resources in many tropical and subtropical Asian countries. Here, we present a chromosome‐level genome assembly of mud crab ( Scylla paramamosain ). The is 1.55 Gb (contig N50 191 kb) length encodes 17,821 proteins. heterozygosity assembled was estimated to be 0.47%. Effective population size analysis suggested an initial large this maintained until 200 thousand years ago. contraction cuticle protein opsin genes compared with Litopenaeus vannamei assumed correlated shell hardness light perception ability, respectively. Furthermore, three chemoreceptor gene families, odorant receptor (OR), gustatory (GR) ionotropic (IR) has no OR shows GR expansion IR genes. numbers families were similar those other decapods but different from two nondecapods insects. In addition, IRs more diversified than nondecapod crustaceans, most expanded clustered antennal clades. These findings might exhibit diverse functions nondecapods, which may compensate for smaller number Decoding S. not only provides insight into genetic changes underpinning ecological traits also valuable information improving breeding aquaculture species.

Language: Английский

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Physiological and molecular mechanisms of insect appendage regeneration

Cell Regeneration, Journal Year: 2023, Volume and Issue: 12(1)

Published: March 2, 2023

Abstract Regeneration, as a fascinating scientific field, refers to the ability of animals replacing lost tissue or body parts. Many metazoan organisms have been reported with regeneration phenomena, but showing evolutionarily variable abilities. As most diverse taxon, hundreds insects show strong appendage ability. The process and are dependent on many factors, including macroscopic physiological conditions microscopic molecular mechanisms. This article reviews research progress internal underlying mechanisms controlling in insects.

Language: Английский

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One genome, multiple phenotypes: decoding the evolution and mechanisms of environmentally induced developmental plasticity in insects

Biochemical Society Transactions, Journal Year: 2023, Volume and Issue: 51(2), P. 675 - 689

Published: March 16, 2023

Plasticity in developmental processes gives rise to remarkable environmentally induced phenotypes. Some of the most striking and well-studied examples plasticity are seen insects. For example, beetle horn size responds nutritional state, butterfly eyespots enlarged response temperature humidity, environmental cues also give queen worker castes eusocial These phenotypes arise from essentially identical genomes an cue during development. Developmental is taxonomically widespread, affects individual fitness, may act as a rapid-response mechanism allowing individuals adapt changing environments. Despite importance prevalence plasticity, there remains scant mechanistic understanding how it works or evolves. In this review, we use key discuss what known about insects identify fundamental gaps current knowledge. We highlight working towards fully integrated diverse range species. Furthermore, advocate for comparative studies evo-devo framework address

Language: Английский

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Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach

iScience, Journal Year: 2022, Volume and Issue: 25(5), P. 104272 - 104272

Published: April 20, 2022

Insects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is heteromeric complex composed ligand-specific receptor and the co-receptor (ORco). In this study, we identified ORco gene cockroach, Periplaneta americana (PameORco), performed RNAi-based functional analysis PameORco. All OSNs basiconic sensilla PameORco received including sex pheromones. trichoid sensilla, PameORco-positive OSN was consistently paired with PameORco-negative tuned to acids. adult cockroaches injected dsRNA at nymphal stage, expression PameORco, odor receptions ORs, its central processing were strongly suppressed. These results provide new insights into molecular basis reception cockroach. long-lasting irreversible effects RNAi would be an effective method for controlling household pest.

Language: Английский

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A single gene integrates sex and hormone regulators into sexual attractiveness

Nature Ecology & Evolution, Journal Year: 2022, Volume and Issue: 6(8), P. 1180 - 1190

Published: July 4, 2022

Language: Английский

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Behavioral and genomic divergence between a generalist and a specialist fly

Cell Reports, Journal Year: 2022, Volume and Issue: 41(7), P. 111654 - 111654

Published: Nov. 1, 2022

The evolution of feeding habits leads to speciation in insects. Bactrocera true fruit flies display diverse across species. We combine behavioral and functional genomic studies probe the divergence between specialist B. minax generalist dorsalis. find that both vision olfaction contribute their respective host preferences, with a dominant effect over short range. Correspondingly, location-related genes are significantly enriched phototransduction pathway, which long-wavelength rhodopsin confers color preference species has been subject selection specialist. also massive expansion olfactory receptors generalist, along signatures conditional expression positive selection. phylogenetic context suggests an ancestrally important role location Bactrocera, as well increased performance plasticity alongside arising generalism.

Language: Английский

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