Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(26)
Published: May 5, 2024
Abstract
Self‐assembling
peptides
have
numerous
applications
in
medicine,
food
chemistry,
and
nanotechnology.
However,
their
discovery
has
traditionally
been
serendipitous
rather
than
driven
by
rational
design.
Here,
HydrogelFinder,
a
foundation
model
is
developed
for
the
design
of
self‐assembling
from
scratch.
This
explores
self‐assembly
properties
molecular
structure,
leveraging
1,377
non‐peptidal
small
molecules
to
navigate
chemical
space
improve
structural
diversity.
Utilizing
111
peptide
candidates
are
generated
synthesized
17
peptides,
subsequently
experimentally
validating
biophysical
characteristics
nine
ranging
1–10
amino
acids—all
achieved
within
19‐day
workflow.
Notably,
two
de
novo‐designed
demonstrated
low
cytotoxicity
biocompatibility,
as
confirmed
live/dead
assays.
work
highlights
capacity
HydrogelFinder
diversify
through
molecules,
offering
powerful
toolkit
paradigm
future
endeavors.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 8, 2025
Tailored
droplet
generation
is
crucial
for
microfluidics
that
involve
samples
of
varying
sizes.
However,
the
absence
precise
predictive
models
forces
platforms
to
rely
on
empiricism
derived
from
extensive
experiments,
underscoring
need
comprehensive
modeling
analysis.
To
address
this,
a
novel
customized
assembled
centrifugal
step
emulsifier
(CASE)
presented
by
incorporating
"jigsaw
puzzles"
design
efficiently
acquire
large-scale
experimental
data.
Numerical
simulations
are
utilized
analyze
fluid
configurations
during
emulsification,
identifying
key
connection
tube
determines
size.
By
training
and
verifying
with
simulation
datasets,
theoretical
model
established
allows
preliminary
size
frequency
an
average
error
rate
4.8%,
successfully
filling
critical
gap
in
existing
field.
This
empowers
CASE
achieve
all-in-one
functionality,
including
pre-design,
generation,
manipulation,
on-site
detection.
As
proof
concept,
multiscale
sample
analysis
ranging
nanoscale
nucleic
acids
microscale
bacteria
3D
cell
spheroids
realized
CASE.
In
summary,
this
platform
offers
valuable
guidance
emulsifiers
promotes
adoption
biochemical
assays.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 19, 2024
Synthetic
extracellular
matrix
(ECM)
mimics
that
can
recapitulate
the
complex
biochemical
and
mechanical
nature
of
native
tissues
are
needed
for
advanced
models
development
disease.
Biomedical
research
has
heavily
relied
on
use
animal-derived
biomaterials,
which
is
now
impeding
their
translational
potential
convoluting
biological
insights
gleaned
from
in
vitro
tissue
models.
Natural
hydrogels
have
long
served
as
a
convenient
effective
cell
culture
tool,
but
advances
materials
chemistry
fabrication
techniques
present
promising
new
avenues
creating
xenogenic-free
ECM
substitutes
appropriate
organotypic
microphysiological
systems.
However,
significant
challenges
remain
synthetic
matrices
approximate
structural
sophistication,
complexity,
dynamic
functionality
tissues.
This
review
summarizes
key
properties
ECM,
discusses
recent
approaches
used
to
systematically
decouple
tune
these
matrices.
The
importance
mechanics,
such
viscoelasticity
plasticity,
also
discussed,
particularly
within
context
organoid
engineered
Emerging
design
strategies
mimic
reviewed,
multi-network
hydrogels,
supramolecular
chemistry,
assembled
monomers.
Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
124(18), P. 10435 - 10508
Published: Sept. 16, 2024
The
mechanical
properties
of
polymer
gels
devote
to
emerging
devices
and
machines
in
fields
such
as
biomedical
engineering,
flexible
bioelectronics,
biomimetic
actuators,
energy
harvesters.
Coupling
network
architectures
interactions
has
been
explored
regulate
supportive
characteristics
gels;
however,
systematic
reviews
correlating
mechanics
interaction
forces
at
the
molecular
structural
levels
remain
absent
field.
This
review
highlights
engineering
gel
a
comprehensive
mechanistic
understanding
regulation.
Molecular
alters
architecture
manipulates
functional
groups/moieties
level,
introducing
various
permanent
or
reversible
dynamic
bonds
dissipative
energy.
usually
uses
monomers,
cross-linkers,
chains,
other
additives.
Structural
utilizes
casting
methods,
solvent
phase
regulation,
mechanochemistry,
macromolecule
chemical
reactions,
biomanufacturing
technology
construct
tailor
topological
structures,
heterogeneous
modulus
compositions.
We
envision
that
perfect
combination
may
provide
fresh
view
extend
exciting
new
perspectives
this
burgeoning
also
summarizes
recent
representative
applications
with
excellent
properties.
Conclusions
are
provided
from
five
aspects
concise
summary,
mechanism,
biofabrication
upgraded
applications,
synergistic
methodology.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(26)
Published: May 5, 2024
Abstract
Self‐assembling
peptides
have
numerous
applications
in
medicine,
food
chemistry,
and
nanotechnology.
However,
their
discovery
has
traditionally
been
serendipitous
rather
than
driven
by
rational
design.
Here,
HydrogelFinder,
a
foundation
model
is
developed
for
the
design
of
self‐assembling
from
scratch.
This
explores
self‐assembly
properties
molecular
structure,
leveraging
1,377
non‐peptidal
small
molecules
to
navigate
chemical
space
improve
structural
diversity.
Utilizing
111
peptide
candidates
are
generated
synthesized
17
peptides,
subsequently
experimentally
validating
biophysical
characteristics
nine
ranging
1–10
amino
acids—all
achieved
within
19‐day
workflow.
Notably,
two
de
novo‐designed
demonstrated
low
cytotoxicity
biocompatibility,
as
confirmed
live/dead
assays.
work
highlights
capacity
HydrogelFinder
diversify
through
molecules,
offering
powerful
toolkit
paradigm
future
endeavors.
