Fundamental Research, Journal Year: 2025, Volume and Issue: unknown
Published: May 1, 2025
Language: Английский
Poultry Science, Journal Year: 2025, Volume and Issue: 104(3), P. 104869 - 104869
Published: Feb. 6, 2025
The intestinal microbiome is essential in regulating host muscle growth and development. Antibiotic treatment commonly used to model dysbiosis of the microbiota, yet limited research addresses relationship between gut microbes yellow-feathered broilers. In this study, Xinghua chickens were administered broad-spectrum antibiotics for eight weeks induce suppression. We investigated relationships using 16S rRNA sequencing transcriptomic analysis. Results indicated that antibiotic significantly reduced body weight, dressed eviscerated breast leg weight. Microbial diversity richness duodenum, jejunum, ileum, cecum decreased. relative abundances Firmicutes, Actinobacteria, Bacteroidetes declined, while Proteobacteria increased. This microbial imbalance led 298 differentially expressed genes (DEGs) tissue, which 67 down-regulated enriched skeletal development, including MYF6, MYBPC1 METTL21C DEGs primarily involved MAPK signaling pathway, calcium ECM-receptor interaction, actin cytoskeleton regulation, nitrogen metabolism. Correlation analysis showed dysregulation cecal had most substantial effect on Furthermore, DNMT3b mRNA expression lowered overall DNA methylation SAM levels, induced changes impacted study demonstrates microbiota influence thereby associated with
Language: Английский
Citations
2
Advanced Materials, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 19, 2025
Abstract Osteoporosis is a widespread condition that induces an inflammatory microenvironment, limiting the effectiveness of conventional therapies and presenting significant challenges for bone defect repair. To address these issues, high‐strength gelatin hydrogel scaffold loaded with roxadustat developed, specifically designed to remodel microenvironment enhance osteoporotic regeneration. By incorporating minimal methacrylated hyaluronic acid (HAMA) into o ‐nitrobenzyl functionalized (GelNB) matrix, fracture strength 10 MPa achieved, providing exceptional structural stability enabling precise fabrication through digital light processing (DLP) 3D printing. Validated cell experiments animal studies, supports adhesion migration, offers excellent tissue compatibility, fully degradable, meeting requirements therapeutic scaffold. Including further enhances scaffold's functionality by regulating via hypoxia‐inducible factor‐1α (HIF‐1α) signaling, significantly improving repair in models. This drug‐loaded effectively addresses inflammation‐induced limitations regenerative capacity affected area, paving way improved outcomes
Language: Английский
Citations
1
Environmental Research, Journal Year: 2024, Volume and Issue: 258, P. 119483 - 119483
Published: June 22, 2024
Language: Английский
Citations
9
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 2, 2024
Abstract The repair of hierarchical osteochondral defects requires complex gradient reconstruction at different levels, with continuous‐gradient mineralization being crucial. Strategies for achieving have rarely been reported. Here, a mineralized hydrogel is prepared using simple gravitational osmosis ≈2 nm amorphous calcium phosphate nanocluster (ACPC) suspension into composite organic frameworks regeneration. During infiltration, ACPC underwent gradual mineralization, resulting in the spontaneous formation hydroxyapatite (HAP). aligned closely normal structure, thereby effectively promoting cartilage and subchondral bone. In layer, compounds improved oxidative stress environment induced by injury complemented extracellular matrix cartilage. bone HAP from continuously intracellular accumulation mesenchymal stem cells (MSCs), activating calcium/calmodulin‐dependent protein kinase 2 (CaMK2) osteogenic differentiation MSCs through signaling pathway. Ultimately, compared nongradient hydrogel, this innovative method exhibited significantly enhanced regeneration capabilities defects, as evidenced metrics, such volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), number (Tb.N). This holistic strategy provides hope field repair.
Language: Английский
Citations
9
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 23, 2024
Osteoarthritis (OA) of the knee is most prevalent degenerative joint condition that places a substantial financial and medical burden on society. However, due to drawbacks such as inefficiency, adverse effects, brief duration action, clinical efficacy current major therapies for OA largely restricted. Therefore, novel medication development highly required address these issues. Numerous studies in recent years have established nanomaterials can be potential effective way overcome challenges. In this review, anatomical distinctions between healthy joints, well advances field treatment are summarized. The limits present therapeutic strategies treating also highlighted, prospects future.
Language: Английский
Citations
7
Nature Metabolism, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 10, 2025
Bone lengthening and fracture repair depend on the anabolic properties of chondrocytes that function in an avascular milieu. The limited supply oxygen nutrients calls into question how biosynthesis redox homeostasis are guaranteed. Here we show glucose metabolism by pentose phosphate pathway (PPP) is essential for endochondral ossification. Loss glucose-6-phosphate dehydrogenase does not affect cell proliferation because reversal non-oxidative PPP produces ribose-5-phosphate. However, decreased NADPH production reduces glutathione recycling, resulting protection against reactive species (ROS) produced during oxidative protein folding. disturbed proteostasis activates unfolded response degradation. Moreover, stress induces ferroptosis, which, together with altered matrix properties, results a chondrodysplasia phenotype. Collectively, these data hypoxia, crucial to produce reducing power confines ROS generated folding thereby controls prevents ferroptosis. Loopmans et al. provides ensure necessary bone lengthening.
Language: Английский
Citations
1
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 160557 - 160557
Published: Feb. 1, 2025
Language: Английский
Citations
1
Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)
Published: Feb. 19, 2025
Angiogenesis and osteogenesis are closely interrelated. The interaction between endothelial bone-forming cells, such as osteoblasts, is crucial for normal bone development repair. Juxtacrine paracrine mechanisms play key roles in cell differentiation towards the osteogenic direction, assuming direct effect of endothelium on differentiation. However, this interplay have yet to be thoroughly studied. Isolated cells (EC) from human umbilical vein osteoblasts (OB) epiphysis femur or tibia were cultured indirect (separated by membrane) contact vitro under conditions. Osteogenic was verified RT-PCR, alizarin red staining. Shotgun proteomics RNA-sequencing used compare both EC OB different co-culture conditions assess EC-OB interplay. To verify role Notch signaling, experiments with modulation performed lentiviral transduction further co-cultivation OB. Additionally, assessed RNA-sequencing. opposite effects depending In contact, enhance differentiation, but cultures, suppress it. Our proteotranscriptomic analysis revealed that osteosuppressive related action factors secreted EC, while osteoinductive properties mediated signaling pathway, which can activated only upon a physical Indeed, co-culture, knockdown Notch1 Notch3 receptors has an inhibitory whereas activation intracellular domain either inductive data indicate dual regulating highlight unique pathway inducing during cell-to-cell interactions. findings study emphasize importance intercellular communication regulation osteoblast maintenance.
Language: Английский
Citations
1
Antioxidants, Journal Year: 2024, Volume and Issue: 13(4), P. 470 - 470
Published: April 17, 2024
Imbalanced osteogenic cell-mediated bone gain and osteoclastic remodeling accelerates the development of osteoporosis, which is leading risk factor disability in elderly. Harmonizing metabolic actions bone-making cells resorbing to mineralized matrix network required maintain mass homeostasis. The tricarboxylic acid (TCA) cycle mitochondria a crucial process for cellular energy production redox canonical TCA enzymes intermediates are indispensable oxidative phosphorylation adenosine triphosphate (ATP) biosynthesis differentiation osteoclast formation. Knockout mouse models identify these enzymes’ roles microarchitecture. In noncanonical processes, metabolites as co-factor or substrate involve epigenetic modification, including histone acetyltransferases, DNA demethylases, RNA m6A affect genomic stability chromatin accessibility cell metabolism formation resorption. genetic manipulation regulators intermediate supplementation compromises age, estrogen deficiency, inflammation-induced loss microstructure deterioration. This review sheds light on functions terms integrity highlights crosstalk pathways skeletal tissue treatment options delaying osteoporosis.
Language: Английский
Citations
5
Osteoarthritis and Cartilage, Journal Year: 2024, Volume and Issue: unknown
Published: June 1, 2024
Language: Английский
Citations
4