Compartmentalization of the host microbiome: how tumor microbiota shapes checkpoint immunotherapy outcome and offers therapeutic prospects DOI Creative Commons

Maximilian Boesch,

Lena Horvath, Florent Baty

et al.

Journal for ImmunoTherapy of Cancer, Journal Year: 2022, Volume and Issue: 10(11), P. e005401 - e005401

Published: Nov. 1, 2022

The host microbiome is polymorphic, compartmentalized, and composed of distinctive tissue microbiomes. While research in the field cancer immunotherapy has provided an improved understanding interaction with gastrointestinal microbiome, significance tumor-associated only recently been grasped. This article provides a state-of-the-art review about sheds light on how local tumor microbiota shapes anticancer immunity influences checkpoint outcome. direct route between cells, immune microenvironment emphasized advocates focus addition to spatially separated gut compartment. Since mechanisms underlying modulation by remain largely elusive, future should dissect pathways involved outline strategies therapeutically modulate microbes their products within microenvironment. A more detailed knowledge governing composition functional quality will improve advance precision medicine for solid tumors.

Language: Английский

Gut microbiota influence immunotherapy responses: mechanisms and therapeutic strategies DOI Creative Commons
Yuting Lu, Xiangliang Yuan, Miao Wang

et al.

Journal of Hematology & Oncology, Journal Year: 2022, Volume and Issue: 15(1)

Published: April 29, 2022

The gut microbiota have long been recognized to play a key role in human health and disease. Currently, several lines of evidence from preclinical clinical research gradually established that the can modulate antitumor immunity affect efficacy cancer immunotherapies, especially immune checkpoint inhibitors (ICIs). Deciphering underlying mechanisms reveals reprogram tumor microenvironment (TME) by engaging innate and/or adaptive cells. Notably, one primary modes which is means metabolites, are small molecules could spread their initial location impact local systemic response promote ICI efficiency. Mechanistic exploration provides novel insights for developing rational microbiota-based therapeutic strategies manipulating microbiota, such as fecal transplantation (FMT), probiotics, engineered microbiomes, specific microbial augment advance age utilization precision medicine.

Language: Английский

Citations

279
Human organoids-on-chips for biomedical research and applications DOI Creative Commons
Hui Wang,

Xiufan Ning,

Feng Zhao

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(2), P. 788 - 818

Published: Jan. 1, 2024

Human organoids-on-chips (OrgOCs) are the synergism of human organoids (HOs) technology and microfluidic organs-on-chips (OOCs).OOCs can mimic extrinsic characteristics organs, such as environmental clues living tissue, while HOs more amenable to biological analysis genetic manipulation.By spatial cooperation, OrgOCs served 3D organotypic models allowing them recapitulate critical tissue-specific properties forecast responses outcomes.It represents a giant leap forward from regular 2D cell monolayers animal in improved ecological niche modeling.In recent years, have offered potential promises for clinical studies advanced preclinical-to-clinical translation medical industrial fields.In this review, we highlight cutting-edge achievements OrgOCs, introduce key features architectures, share revolutionary applications basic biology, disease modeling, preclinical assay precision medicine.Furthermore, discuss how combine wide range disciplines with accelerate translational applications, well challenges opportunities biomedical research applications.

Language: Английский

Citations

23
The role of animal hosts in shaping gut microbiome variation DOI Creative Commons
Elisa Maritan, Andrea Quagliariello, Enric Frago

et al.

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)

Published: March 18, 2024

Millions of years co-evolution between animals and their associated microbial communities have shaped diversified the nature relationship. Studies continue to reveal new layers complexity in host–microbe interactions, fate which depends on a variety different factors, ranging from neutral processes environmental factors local dynamics. Research is increasingly integrating ecosystem-based approaches, metagenomics mathematical modelling disentangle individual contribution ecological microbiome evolution. Within this framework, host are known be among dominant drivers composition animal species. However, extent they shape assembly evolution remains unclear. In review, we summarize our understanding how drive these dynamics conserved vary across taxa. We conclude by outlining key avenues for research highlight need implementation modifications existing theory fully capture host-associated microbiomes. This article part theme issue ‘Sculpting microbiome: determine respond colonization’.

Language: Английский

Citations

20
Microbiomes of microscopic marine invertebrates do not reveal signatures of phylosymbiosis DOI
Vittorio Boscaro, Corey C. Holt, Niels W. L. Van Steenkiste

et al.

Nature Microbiology, Journal Year: 2022, Volume and Issue: 7(6), P. 810 - 819

Published: May 26, 2022

Language: Английский

Citations

59
Microbiome breeding: conceptual and practical issues DOI Creative Commons
Ulrich G. Mueller, Timothy A. Linksvayer

Trends in Microbiology, Journal Year: 2022, Volume and Issue: 30(10), P. 997 - 1011

Published: May 18, 2022

Language: Английский

Citations

44
Drivers of gut microbiome variation within and between groups of a wild Malagasy primate DOI Creative Commons
Katja Rudolph, Dominik Schneider, Claudia Fichtel

et al.

Microbiome, Journal Year: 2022, Volume and Issue: 10(1)

Published: Feb. 9, 2022

Various aspects of sociality can benefit individuals' health. The host social environment and its relative contributions to the host-microbiome relationship have emerged as key topics in microbial research. Yet, understanding mechanisms that lead structural variation microbiome, collective metacommunity an animal's network, remains difficult since multiple processes operate simultaneously within among animal networks. Here, we examined potential drivers convergence gut microbiome on scales seven neighbouring groups wild Verreaux's sifakas (Propithecus verreauxi) - a folivorous primate Madagascar. Over four field seasons, collected 519 faecal samples 41 animals determined communities via 16S 18S rRNA gene amplicon analyses. First, whether group members share more similar microbiota if diet, home range overlap, or habitat similarity drive between-group communities, accounting for seasonality. Next, within-group by examining effects contact rates, male rank, maternal relatedness. To explore intrinsic community structure, investigated age, sex, glucocorticoid metabolites, female reproductive state. We found differ alpha diversity, while none environmental predictors explained patterns variation. Maternal relatedness played important role homogeneity may also explain why adult shared least microbiota. Also, dominant males differed their bacterial composition from mates, which might be driven rank-related differences physiology scent-marking behaviours. Links state, metabolites were not detected. Environmental factors define general set-up population-specific microbiota, but stronger impact this species. Video abstract.

Language: Английский

Citations

39
Into the microbial niche DOI Creative Commons
Lucie Malard, Antoine Guisan

Trends in Ecology & Evolution, Journal Year: 2023, Volume and Issue: 38(10), P. 936 - 945

Published: May 24, 2023

The environmental niche concept describes the distribution of a taxon in environment and can be used to understand community dynamics, biological invasions, impact changes. uses applications are still restricted microbial ecology, largely due complexity systems associated methodological limitations. development shotgun metagenomics metatranscriptomics opens new ways investigate by focusing on metabolic within space. Here, we propose framework, which, defining fundamental realised microorganisms, has potential not only provide novel insights into habitat preferences metabolism associated, but also inform plasticity, shifts, invasions.

Language: Английский

Citations

37
Reactive oxygen species are regulated by immune deficiency and Toll pathways in determining the host specificity of honeybee gut bacteria DOI Creative Commons

Lizhen Guo,

Junbo Tang, Min Tang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(33)

Published: Aug. 9, 2023

Host specificity is observed in gut symbionts of diverse animal lineages. But how hosts maintain while rejecting their close relatives remains elusive. We use eusocial bees and codiversified bacteria to understand host regulation driving symbiotic specificity. The cross-inoculation bumblebee Gilliamella induced higher prostaglandin the honeybee gut, promoting a pronounced response through immune deficiency (IMD) Toll pathways. Gene silencing vitamin C treatments indicate that reactive oxygen species (ROS), not antimicrobial peptides, acts as effector inhibiting non-native strain. Quantitative PCR RNAi further reveal regulatory function IMD pathways, which Relish dorsal-1 may regulate Dual Oxidase ( Duox ) for ROS production. Therefore, maintains by creating hostile environment exotic bacteria, differential its system, reflecting co-opting existing machinery evolved combat pathogens.

Language: Английский

Citations

33
Host species and habitat shape fish-associated bacterial communities: phylosymbiosis between fish and their microbiome DOI Creative Commons
Javad Sadeghi, Subba Rao Chaganti, Timothy B. Johnson

et al.

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: Nov. 20, 2023

While many studies have reported that the structure of gut and skin microbiota is driven by both species-specific habitat-specific factors, relative importance host-specific versus environmental factors in wild vertebrates remains poorly understood. The aim this study was to determine diversity composition fish skin, gut, surrounding water bacterial communities (hereafter referred as microbiota) assess extent which host habitat phylogeny predict similarity. Skin swabs samples from 334 belonging 17 species were sampled three Laurentian Great Lakes (LGLs) habitats (Detroit River, Lake Erie, Ontario). We also collected filtered at time collection. analyzed community using 16S metabarcoding tested for variation.We found distinct microbiota, although more closely resembled microbiota. (sample location), habitat, diet, shape promote divergence or convergence Since significantly affected (separately effects), we phylosymbiosis pairwise phylogenetic distance dissimilarity. significant effects on dissimilarity, consistent with perhaps reflecting longstanding co-evolutionary relationship between their microbiomes.Analyzing mucus across diverse complex natural ecosystems such LGLs provides insights into potential microbiome, ultimately health, host. Video Abstract.

Language: Английский

Citations

29
Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet? DOI Creative Commons
Katalin Demeter, Rita Linke, Elisenda Ballesté

et al.

FEMS Microbiology Reviews, Journal Year: 2023, Volume and Issue: 47(4)

Published: June 7, 2023

The impacts of nucleic acid-based methods - such as PCR and sequencing to detect analyze indicators, genetic markers or molecular signatures microbial faecal pollution in health-related water quality research were assessed by rigorous literature analysis. A wide range application areas study designs has been identified since the first more than 30 years ago (>1100 publications). Given consistency assessment types, we suggest defining this emerging part science a new discipline: diagnostics (GFPD) Undoubtedly, GFPD already revolutionized detection (i.e., traditional alternative general indicator/marker analysis) source tracking host-associated analysis), current core applications. is also expanding many other areas, including infection health risk assessment, evaluation treatment, support wastewater surveillance. In addition, storage DNA extracts allows for biobanking, which opens up perspectives. tools can be combined with cultivation-based standardized indicator enumeration, pathogen detection, various environmental data an integrated analysis approach. This comprehensive meta-analysis provides scientific status quo field, trend analyses statistics, outlining discusses benefits challenges GFPD.

Language: Английский

Citations

25