Social networking at the microbiome-host interface

Infection and Immunity, Journal Year: 2023, Volume and Issue: 91(9)

Published: Aug. 18, 2023

Microbial species colonizing host ecosystems in health or disease rarely do so alone. Organisms conglomerate into dynamic heterotypic communities biofilms which interspecies and interkingdom interactions drive functional specialization of constituent shape community properties, including nososymbiocity pathogenic potential. Cell-to-cell binding, exchange signaling molecules, nutritional codependencies can all contribute to the emergent properties these communities. Spatial constraints defined by architecture also determine overall function. Multilayered thus occur between individual pairs organisms, relative impact be determined contextual cues. Host responses on surfaces are driven collective action community. Additionally, range extended bacteria utilizing cells diet indirectly directly influence other organisms microenvironment. In contexts where transition a dysbiotic state, their quasi-organismal nature imparts adaptability availability facilitates resistance immune effectors and, moreover, exploits inflammatory acidic microenvironments for persistence.

Language: Английский

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Active Density Pattern Formation in Bacterial Binary Mixtures

PRX Life, Journal Year: 2024, Volume and Issue: 2(2)

Published: April 4, 2024

This study identified a new mechanism of active self-organization in microbial communities by which motile bacteria induce non-equilibrium spatial patterns non-motile ones through surface-swimming-induced fluid flows.

Language: Английский

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The impact of phage and phage resistance on microbial community dynamics

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(4), P. e3002346 - e3002346

Published: April 22, 2024

Where there are bacteria, will be bacteriophages. These viruses known to important players in shaping the wider microbial community which they embedded, with potential implications for human health. On other hand, bacteria possess a range of distinct immune mechanisms that provide protection against bacteriophages, including mutation or complete loss phage receptor, and CRISPR-Cas adaptive immunity. While our previous work showed how may impact resistance evolution, little is about inverse, namely interactions between phages these different affect embedded. Here, we conducted 10-day, fully factorial evolution experiment examine structure dynamics an artificial four-species bacterial includes either Pseudomonas aeruginosa wild-type isogenic mutant unable evolve through CRISPR-Cas. Additionally, used mathematical modelling explore ecological underlying full behaviour, as well identify general principles governing impacts on dynamics. Our results show drastically altered by addition phage, Acinetobacter baumannii becoming dominant species P . being driven nearly extinct, whereas outcompetes absence phage. Moreover, find strain ability CRISPR-based generally does better when presence A , but this benefit largely lost over time extinct. Finally, pairwise data alone insufficient community, both without highlighting importance higher order multispecies complex communities. Combined, clearly illustrate targeting allows competitive release strongest competitor while also contributing diversity maintenance potentially preventing reinvasion target species, underline mapping composition before therapeutically applying

Language: Английский

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Material Substrate Physical Properties Control Pseudomonas aeruginosa Biofilm Architecture

mBio, Journal Year: 2023, Volume and Issue: 14(2)

Published: Feb. 14, 2023

In the wild, bacteria are most frequently found in form of multicellular structures called biofilms. Biofilms grow at surface abiotic and living materials with wide-ranging mechanical properties. The opportunistic pathogen Pseudomonas aeruginosa forms biofilms on indwelling medical devices soft tissues, including burn wounds airway mucosa. Despite critical role substrates foundation biofilms, we still lack a clear understanding how material mechanics regulate their architecture physiology resident bacteria. Here, demonstrate that physical properties hydrogel define P. biofilm architecture. We show mesh size regulates twitching motility, exploration mechanism priming ultimately controlling organization single cells community. resulting architectural transitions increase aeruginosa's tolerance to colistin, last-resort antibiotic. addition, regulation motility affects clonal lineages, so more mixed relatively denser materials. Our results thereby establish as factor dramatically architecture, antibiotic efficacy, evolution population.

Language: Английский

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Spatial self-organization of metabolism in microbial systems: A matter of enzymes and chemicals

Cell Systems, Journal Year: 2023, Volume and Issue: 14(2), P. 98 - 108

Published: Feb. 1, 2023

Language: Английский

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Echinacoside reduces intracellular c-di-GMP levels and potentiates tobramycin activity against Pseudomonas aeruginosa biofilm aggregates

npj Biofilms and Microbiomes, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 7, 2025

Abstract Cyclic diguanylate (c-di-GMP) is a central biofilm regulator in Pseudomonas aeruginosa , where increased intracellular levels promote formation and antibiotic tolerance. Targeting the c-di-GMP network may be promising anti-biofilm approach, but most strategies studied so far aimed at eliminating surface-attached biofilms, while vivo P. biofilms often occur as suspended aggregates. Here, expression profile of metabolism-related genes was analysed among 32 strains grown aggregates synthetic cystic fibrosis sputum. The cyclase SiaD proved essential for auto-aggregation under vivo-like conditions. Virtual screening predicted high binding affinity echinacoside towards active site SiaD. Echinacoside reduced aggregate sizes potentiated tobramycin activity against >80% tested. This synergism also observed -infected 3-D alveolar epithelial cells murine lungs, demonstrating echinacoside’s potential an adjunctive therapy recalcitrant infections.

Language: Английский

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Shining Light on Oral Biofilm Fluorescence In Situ Hybridization (FISH): Probing the Accuracy of In Situ Biogeography Studies

Molecular Oral Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

ABSTRACT The oral biofilm has been instrumental in advancing microbial research and enhancing our understanding of health disease. Recent developments next‐generation sequencing have provided detailed insights into the composition microbiome, enabling species‐level analyses interactions. Fluorescence situ hybridization (FISH) especially valuable for studying spatial organization these microbes, revealing intricate arrangements such as “corncob” structures that highlight close bacterial As more genetic sequence data become available, specificity accuracy existing FISH probes used biogeographical studies require reevaluation. This study examines performance commonly species‐specific probes, designed to differentiate microbes within biofilms, when applied vitro an expanded set strains. Our findings reveal several is compromised, with cross‐species being common than previously assumed. Notably, we demonstrate associations particularly involving Streptococcus Corynebacterium , may need be reassessed align latest metagenomic data.

Language: Английский

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Growth rates of bacteria in vivo

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

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Monoclonal antibodies that target extracellular DNABII proteins or the type IV pilus of nontypeable Haemophilus influenzae (NTHI) worked additively to disrupt 2-genera biofilms

Biofilm, Journal Year: 2022, Volume and Issue: 4, P. 100096 - 100096

Published: Dec. 1, 2022

The biofilm state is the preferred lifestyle of bacteria in nature. Within a biofilm, resident are protected from environmental stresses, antibiotics and other antimicrobials, including those due to multiple immune effectors their host during conditions disease. Thereby, biofilms contribute significantly pathogenicity, recalcitrance clearance chronicity/recurrence bacterial diseases, diseases respiratory tract. In absence highly effective, biofilm-targeted therapeutics, commonly prescribed attempt treat these however, light canonical resistance biofilm-resident antibiotic-mediated killing, this ineffectual practice often fails resolve diseased condition contributes global threat rising antimicrobial resistance. Nontypeable Haemophilus influenzae common tract disease co-pathogen, present partnership with airway pathogens. Herein we aspired determine whether either two monoclonal antibodies developed, one specific for NTHI [directed against majority subunit (PilA) type IV pilus (T4P) NTHI] able act agnostically on all tested date (directed structural protein matrix, DNABII protein), were disrupt 2-genera wherein co-partnered another pathogen. These monoclonals singly as well when within an antibody cocktail. directed antigen PilA was only effective single species not formed by unrelated species. However, any 5 pathogens here (Burkholderia cenocepacia, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumoniae or Moraxella catarrhalis), exclusively NTHI-directed biofilms. Conversely, protective epitopes protein, disrupted biofilms, which reflected universal presence matrices. greatest release both uniformly achieved incubation 1:1 cocktail monoclonals. data support use approach patients could be treated therapeutic its co-pathogens killed traditional and/or effectors.

Language: Английский

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One Earth: The Equilibrium between the Human and the Bacterial Worlds

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(20), P. 15047 - 15047

Published: Oct. 10, 2023

Misuse and abuse of antibiotics on humans, cattle, crops have led to the selection multi-resistant pathogenic bacteria, most feared ‘superbugs’. Infections caused by superbugs are progressively difficult treat, with a subsequent increase in lethality: toll human lives is predicted reach 10 million 2050. Here we review three concepts linked growing resistance antibiotics, namely (i) Resistome, which refers collection bacterial genes that confer (ii) Mobilome, includes all mobile genetic elements participate spreading antibiotic among bacteria horizontal gene transfer processes, (iii) Nichome, set expressed when try colonize new niches. We also discuss strategies can be used tackle infections propose an entente cordiale world so instead war destruction ‘fierce enemy’ achieve peaceful coexistence (the One Earth concept) between worlds. This, turn, will contribute microbial biodiversity, crucial globally changing climate due anthropogenic activities.

Language: Английский

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