Biochemical Pathways of Cellular Mechanosensing/Mechanotransduction and Their Role in Neurodegenerative Diseases Pathogenesis DOI Creative Commons
Ilaria Tortorella, Chiara Argentati, Carla Emiliani

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(19), P. 3093 - 3093

Published: Oct. 1, 2022

In this review, we shed light on recent advances regarding the characterization of biochemical pathways cellular mechanosensing and mechanotransduction with particular attention to their role in neurodegenerative disease pathogenesis. While mechanistic components these are mostly uncovered today, crosstalk between mechanical forces soluble intracellular signaling is still not fully elucidated. Here, recapitulate general concepts mechanobiology mechanisms that govern processes, examine stimuli response, highlighting effect organelles' homeostasis dysfunction. particular, discuss current knowledge about translation mechanosignaling into signaling, focusing those diseases encompass metabolic accumulation mutant proteins have as primary characteristics formation pathological aggregates, such Alzheimer's Disease, Huntington's Amyotrophic Lateral Sclerosis Parkinson's Disease. Overall, findings elucidate how may be crucial understand pathogenic underlying emphasize importance for identifying potential therapeutic targets.

Language: Английский

iPSC-based disease modeling and drug discovery in cardinal neurodegenerative disorders DOI Creative Commons
Hideyuki Okano, Satoru Morimoto

Cell stem cell, Journal Year: 2022, Volume and Issue: 29(2), P. 189 - 208

Published: Feb. 1, 2022

Language: Английский

Citations

122
Engineering Electrodes with Robust Conducting Hydrogel Coating for Neural Recording and Modulation DOI
Jiajun Zhang, Lulu Wang, Yuhua Xue

et al.

Advanced Materials, Journal Year: 2022, Volume and Issue: 35(3)

Published: Nov. 18, 2022

Abstract Coating conventional metallic electrodes with conducting polymers has enabled the essential characteristics required for bioelectronics, such as biocompatibility, electrical conductivity, mechanical compliance, and capacity structural chemical functionalization of bioelectrodes. However, fragile interface between polymer electrode in wet physiological environment greatly limits their utility reliability. Here, a general yet reliable strategy to seamlessly hydrogel coatings is established, featuring tissue‐like modulus, highly‐desirable electrochemical properties, robust interface, long‐term Numerical modeling reveals role toughening mechanism, synergy covalent anchorage long‐chain polymers, cross‐linking, improving robustness interface. Through vivo implantation freely‐moving mouse models, it shown that stable electrophysiological recording can be achieved, while hydrogel–electrode remains during low‐voltage stimulation. This simple versatile design addresses long‐standing technical challenges functional bioelectrode engineering, opens up new avenues next‐generation diagnostic brain‐machine interfaces.

Language: Английский

Citations

77
Hydrogen‐Bonded Organic Framework (HOF)‐Based Single‐Neural Stem Cell Encapsulation and Transplantation to Remodel Impaired Neural Networks DOI

Dongqin Yu,

Haochen Zhang, Zhenqi Liu

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(28)

Published: April 6, 2022

Abstract Herein we present a new way to encapsulate neural stem cells (NSCs) by using hydrogen‐bonded organic frameworks (HOFs) overcome the common causes of low therapeutic efficacy during NSC transplantation: 1) loss fundamental cell properties, “stemness”, before transplantation, 2) cytomembrane damage and 3) apoptosis due oxidative stress after transplantation. Porous carbon nanospheres (PCNs) are doped into HOF shell process mineralization endow cellular exoskeletons with hierarchical hydrogen bonds, ability resist catalase superoxide dismutase‐like activities PCN. Under NIR‐II irradiation, thermal‐responsive bonds dissociate release NSCs. Stereotactic transplanting encapsulated brain an Alzheimer's disease (AD) mouse model further verifies that our design can enhance viability, promote neurogenesis, ameliorate cognitive impairment. As first example HOFs NSCs, this work may inspire HOF‐based neurogenesis behavioral symptoms associated AD.

Language: Английский

Citations

74
Spatially controlled construction of assembloids using bioprinting DOI Creative Commons
Julien G. Roth, Lucia G. Brunel, Michelle S. Huang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: July 19, 2023

Abstract The biofabrication of three-dimensional (3D) tissues that recapitulate organ-specific architecture and function would benefit from temporal spatial control cell-cell interactions. Bioprinting, while potentially capable achieving such control, is poorly suited to organoids with conserved cytoarchitectures are susceptible plastic deformation. Here, we develop a platform, termed Spatially Patterned Organoid Transfer (SPOT), consisting an iron-oxide nanoparticle laden hydrogel magnetized 3D printer enable the controlled lifting, transport, deposition organoids. We identify cellulose nanofibers as both ideal biomaterial for encasing magnetic nanoparticles shear-thinning, self-healing support maintaining positioning facilitate generation assembloids. leverage SPOT create precisely arranged assembloids composed human pluripotent stem cell-derived neural patient-derived glioma In doing so, demonstrate potential platform construct which key developmental processes disease etiologies.

Language: Английский

Citations

65
Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons DOI Creative Commons
Zaida Álvarez, José Antonio Ortega, Kohei Sato

et al.

Cell stem cell, Journal Year: 2023, Volume and Issue: 30(2), P. 219 - 238.e14

Published: Jan. 12, 2023

Language: Английский

Citations

51
Tunable hydrogel viscoelasticity modulates human neural maturation DOI Creative Commons
Julien G. Roth, Michelle S. Huang, Renato S. Navarro

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(42)

Published: Oct. 20, 2023

Human-induced pluripotent stem cells (hiPSCs) have emerged as a promising in vitro model system for studying neurodevelopment. However, current models remain limited their ability to incorporate tunable biomechanical signaling cues imparted by the extracellular matrix (ECM). The native brain ECM is viscoelastic and stress-relaxing, exhibiting time-dependent response an applied force. To recapitulate remodelability of neural ECM, we developed family protein-engineered hydrogels that exhibit stress relaxation rates. hiPSC-derived progenitor (NPCs) encapsulated within these gels underwent rate-dependent maturation. Specifically, NPCs with faster rates extended longer, more complex neuritic projections, exhibited decreased metabolic activity, expressed higher levels genes associated By inhibiting actin polymerization, observed projections concomitant decrease maturation gene expression. Together, results suggest microenvironmental viscoelasticity sufficient bias human NPC

Language: Английский

Citations

47
Integrating organoids and organ-on-a-chip devices DOI
Yimu Zhao, Shira Landau, Sargol Okhovatian

et al.

Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(7), P. 588 - 608

Published: July 2, 2024

Language: Английский

Citations

32
Organic transistor‐based integrated circuits for future smart life DOI Creative Commons
Yifan Xie,

Chenming Ding,

Qingqing Jin

et al.

SmartMat, Journal Year: 2024, Volume and Issue: 5(4)

Published: Jan. 2, 2024

Abstract With the rapid development of advanced technologies in Internet Things era, higher requirements are needed for next‐generation electronic devices. Fortunately, organic thin film transistors (OTFTs) provide an effective solution skin and flexible wearable devices due to their intrinsic features mechanical flexibility, lightweight, simple fabrication process, good biocompatibility. So far considerable efforts have been devoted this research field. This article reviews recent advances various promising state‐of‐the‐art OTFTs as well related integrated circuits with main focuses on: (I) material categories high‐mobility semiconductors both individual circuits; (II) device architectures processing techniques large‐area fabrication; (III) important performance metrics realization digital analog future smart life; (IV) applicable analytical models design flow accelerate circuit design. In addition, emerging challenges OTFT‐based circuits, such transistor uniformity stability also discussed, possible methods solve these problems at levels summarized.

Language: Английский

Citations

27
Rational design of electrically conductive biomaterials toward excitable tissues regeneration DOI
Guoxu Zhao, Hongwei Zhou, Guorui Jin

et al.

Progress in Polymer Science, Journal Year: 2022, Volume and Issue: 131, P. 101573 - 101573

Published: June 4, 2022

Language: Английский

Citations

43
Morphogenesis and development of human telencephalic organoids in the absence and presence of exogenous extracellular matrix DOI Creative Commons
Catarina Martins‐Costa, Vincent A. Pham, Jaydeep Sidhaye

et al.

The EMBO Journal, Journal Year: 2023, Volume and Issue: 42(22)

Published: Oct. 16, 2023

The establishment and maintenance of apical-basal polarity is a fundamental step in brain development, instructing the organization neural progenitor cells (NPCs) developing cerebral cortex. Particularly, basally located extracellular matrix (ECM) crucial for this process. In vitro, epithelial polarization can be achieved via endogenous ECM production, or exogenous supplementation. While neuroepithelial development recapitulated organoids, effects different sources tissue morphogenesis remain underexplored. Here, we show that exposure to solubilized basement membrane substrate, Matrigel, at early stages causes rapid rearrangement architecture. cultures exposed pure components unexposed any ECM, acquisition slower driven by production. After onset neurogenesis, architecture neuronal differentiation are largely independent initial source, but Matrigel has long-lasting on patterning. These results advance knowledge mechanisms exogenously endogenously guided morphogenesis, demonstrating self-sustainability processes.

Language: Английский

Citations

30