Cell stem cell, Journal Year: 2024, Volume and Issue: 31(7), P. 945 - 946
Published: July 1, 2024
Language: Английский
Cell, Journal Year: 2024, Volume and Issue: 187(11), P. 2855 - 2874.e19
Published: April 23, 2024
Language: Английский
Citations
19
Cell stem cell, Journal Year: 2024, Volume and Issue: 31(7), P. 1058 - 1071.e5
Published: May 31, 2024
The hypoblast is an essential extraembryonic tissue set aside within the inner cell mass in blastocyst. Research with human embryos challenging. Thus, stem models that reproduce differentiation provide valuable alternatives. We show here naive pluripotent (PSC) to proceeds via reversion a transitional ICM-like state from which emerges concordance trajectory blastocysts. identified window when fibroblast growth factor (FGF) signaling critical for specification. Revisiting FGF revealed inhibition early blastocyst suppresses formation. In vitro, induction of synergistically enhanced by limiting trophectoderm and epiblast fates. This finding revises previous reports establishes conservation lineage specification between mice humans. Overall, this study demonstrates utility PSC-based elucidating mechanistic features embryogenesis.
Language: Английский
Citations
16
Developmental Biology, Journal Year: 2024, Volume and Issue: 509, P. 43 - 50
Published: Feb. 5, 2024
Understanding the processes and mechanisms underlying early human embryo development has become an increasingly active important area of research. It potential for insights into clinical issues such as pregnancy loss, origins congenital anomalies developmental adult disease, well fundamental biology. Improved culture systems preimplantation embryos, combined with new tools single cell genomics live imaging, are providing similarities differences between mouse development. However, access to material is still restricted extended embryos regulatory ethical concerns. Stem cell-derived models different phases can potentially overcome these limitations provide a scalable source explore postimplantation stages To date, clearly incomplete replicas normal but future technological improvements be envisaged. The environment studies remains fully resolved.
Language: Английский
Citations
12
Stem Cell Reports, Journal Year: 2025, Volume and Issue: unknown, P. 102419 - 102419
Published: Feb. 1, 2025
Highlights•A biallelic, dual-colored fluorescent reporter at the imprinted SNRPN locus in hPSCs•Biallelic expression is rapidly induced during primed-to-naive resetting•Acquisition of biallelic irreversible upon re-priming•ZFP57 overexpression mitigates imprint erasure resettingSummaryNaive human pluripotent stem cells (hPSCs) model pre-implantation epiblast. However, parent-specific epigenetic marks (imprints) are eroded naive hPSCs, which represents an important deviation from epiblast vivo. To track dynamics resetting real time, we established a both alleles locus. During resetting, becomes most cells, and re-priming. We utilized this live-cell to evaluate chemical genetic strategies minimize erasure. Decreasing level MEK/ERK inhibition or overexpressing KRAB zinc-finger protein ZFP57 protected subset imprints resetting. Combining these two levels further extent than either strategy alone. This study offers experimental tool enhance stability transitions between states vitro.Graphical abstract
Language: Английский
Citations
1
Development, Journal Year: 2025, Volume and Issue: 152(7)
Published: April 1, 2025
ABSTRACT Pluripotency, the capacity to generate all cells of body, is a defining property transient population epiblast found in pre-, peri- and post-implantation mammalian embryos. As development progresses, undergo dynamic transitions pluripotency states, concurrent with specification extra-embryonic embryonic lineages. Recently, stem cell-based models pre- human have been developed using that capture key properties at different developmental stages. Here, we review early primate development, comparing states vivo cultured pluripotent representative these states. We consider how status starting influences embryo and, turn, what can learn about epiblast. Finally, discuss limitations questions arising from pioneering studies this emerging field.
Language: Английский
Citations
1
Cell, Journal Year: 2025, Volume and Issue: unknown
Published: May 1, 2025
The amnion, an extra-embryonic tissue in mammalian embryos, is thought to provide crucial signaling, structural, and nutritional support during pregnancy. Despite its pivotal importance, studying human amnion formation function has been hampered by the lack of accurate vitro models. Here, we present embryonic stem cell-derived 3D model post-gastrulation amnioids (PGAs), that faithfully recapitulates development up 4 weeks post-fertilization, closely mimicking functional traits amniotic sac. PGAs self-organize, forming yolk sac, are surrounded mesoderm. Using PGAs, show GATA3 required sufficient for amniogenesis autoregulatory feedback loop governs formation, whereby signals promote specification. reproducibility scalability PGA system, with precise cellular, integrity, opens avenues investigating embryo-amnion interactions beyond gastrulation offers ideal platform large-scale pharmacological clinical studies.
Language: Английский
Citations
1
Cell, Journal Year: 2024, Volume and Issue: 187(13), P. 3194 - 3219
Published: June 1, 2024
Developing functional organs from stem cells remains a challenging goal in regenerative medicine. Existing methodologies, such as tissue engineering, bioprinting, and organoids, only offer partial solutions. This perspective focuses on two promising approaches emerging for engineering human cells: cell-based embryo models interspecies organogenesis. Both exploit the premise of guiding to mimic natural development. We begin by summarizing what is known about early development blueprint recapitulating organogenesis both chimeras. The latest advances fields are discussed before highlighting technological knowledge gaps be addressed developing could achieved using approaches. conclude discussing challenges facing modeling outlining future prospects advancing toward generation tissues basic research translational applications.
Language: Английский
Citations
8
Cell stem cell, Journal Year: 2024, Volume and Issue: 31(10), P. 1398 - 1418
Published: Oct. 1, 2024
Language: Английский
Citations
5
Cell stem cell, Journal Year: 2024, Volume and Issue: 31(2), P. 155 - 157
Published: Feb. 1, 2024
Language: Английский
Citations
4
Science China Life Sciences, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 17, 2025
Language: Английский
Citations
0