Functional architecture of intracellular oscillations in hippocampal dendrites DOI Creative Commons
Zhenrui Liao, Kevin C. Gonzalez,

Deborah M. Li

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 26, 2024

Fast electrical signaling in dendrites is central to neural computations that support adaptive behaviors. Conventional techniques lack temporal and spatial resolution the ability track underlying membrane potential dynamics present across complex three-dimensional dendritic arbor vivo. Here, we perform fast two-photon imaging of somatic single pyramidal cells CA1 region mouse hippocampus during awake behavior. We study subthreshold suprathreshold events throughout vivo by combining voltage with simultaneous local field recording, post hoc morphological reconstruction, a navigation task. systematically quantify modulation event rates locomotion distinct regions, report an advancing gradient theta phase along basal-tuft axis, describe predominant hyperpolarization sharp-wave ripples. Finally, find tuning representations dynamically reorganizes following place formation. Our data reveal how organization maps onto anatomy tree behavior, oscillatory network, functional cell states. Neurons receive their input three dimensions via dendrites, but activity organized unknown. authors work out rules govern this 3D structure different brain

Language: Английский

Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight DOI Creative Commons
Lee Min Leong, Douglas A. Storace

Neurophotonics, Journal Year: 2024, Volume and Issue: 11(03)

Published: Jan. 17, 2024

Genetically encoded voltage indicators (GEVIs) are protein-based optical sensors that allow for measurements from genetically defined populations of neurons. Although in vivo imaging the mammalian brain with early generation GEVIs was difficult due to poor membrane expression and low signal-to-noise ratio, newer more sensitive have begun make them useful answering fundamental questions neuroscience. We discuss principles using calcium indicators, both tools neuronal activity, review some recent mechanistic advances led GEVI improvements. provide an overview mouse olfactory bulb (OB) studies ArcLight study different cell types within widefield two-photon microscopy. Specific emphasis is placed on begin concentration coding OB, how interpret signals population brain, future developments will push field forward.

Language: Английский

Citations

3
An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non‐Excitable Cells DOI Creative Commons
Philipp Rühl,

Anagha G. Nair,

Namrata Gawande

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(20)

Published: March 25, 2024

Abstract Most animal cell types are classified as non‐excitable because they do not generate action potentials observed in excitable cells, such neurons and muscle cells. Thus, resolving voltage signals cells demands sensors with exceptionally high sensitivity. In this study, the ultrabright, ultrasensitive, calibratable genetically encoded sensor rEstus is developed using structure‐guided engineering. most sensitive resting range of offers a 3.6‐fold improvement brightness change for fast spikes over its precursor ASAP3. Using rEstus, it uncovered that membrane several lines (A375, HEK293T, MCF7) undergoes spontaneous endogenous alterations on second to millisecond timescale. Correlation analysis these optically recorded provides direct, real‐time readout electrical cell–cell coupling, showing visually connected A375 HEK293T also largely electrically connected, while MCF7 only weakly coupled. The presented work enhanced tools methods non‐invasive imaging living demonstrates limited but occur variety types.

Language: Английский

Citations

3
Neurophotonics beyond the surface: unmasking the brain’s complexity exploiting optical scattering DOI Creative Commons
Fei Xia, Caio Vaz Rimoli, Walther Akemann

et al.

Neurophotonics, Journal Year: 2024, Volume and Issue: 11(S1)

Published: April 12, 2024

The intricate nature of the brain necessitates application advanced probing techniques to comprehensively study and understand its working mechanisms. Neurophotonics offers minimally invasive methods probe using optics at cellular even molecular levels. However, multiple challenges persist, especially concerning imaging depth, field view, speed, biocompatibility. A major hindrance solving these in is scattering brain. This perspective highlights potential complex media optics, a specialized area focused on light propagation materials with heterogeneous optical properties, advancing improving neuronal readouts for structural recordings activity. Key strategies include wavefront shaping computational sensing that exploit properties enhanced performance. We discuss merger two fields as well perspectives toward longer term vivo applications.

Language: Английский

Citations

3
Real-time self-supervised denoising for high-speed fluorescence neural imaging DOI Creative Commons
Biqin Dong, Yiqun Wang, Yuanjie Gu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Abstract Self-supervised denoising methods significantly enhance the signal-to-noise ratio in fluorescence neural imaging, yet real-time solutions remain scarce high-speed applications. Here, we present FrAme-multiplexed SpatioTemporal learning strategy (FAST), a deep-learning framework designed for including vivo calcium, voltage, and volumetric time-lapse imaging. FAST balances spatial temporal redundancy across neighboring pixels, preserving structural fidelity while preventing over-smoothing of rapidly evolving signals. Utilizing an ultra-light convolutional network, enables processing at speeds exceeding 1,000 frames per second. We also introduce intuitive graphical user interface (GUI) that integrates into standard imaging workflows, providing tool recorded activity enabling downstream analysis neuroscience research requires millisecond-scale precision, particularly closed-loop studies.

Language: Английский

Citations

0
Photon-efficient camera with in-sensor computing DOI Creative Commons
Yanqiu Guan, Haochen Li, Yi Zhang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 3, 2025

Image sensors with internal computing capabilities fuse sensing and to significantly reduce the power consumption latency of machine vision tasks. Linear photodetectors such as 2D semiconductors tunable electrical optical properties enable in-sensor for multiple functions. In-sensor at single-photon level is much more plausible but has not yet been achieved. Here, we demonstrate a photon-efficient camera based on superconducting nanowire array detector four programmable dimensions including photon count rate, response time, pulse amplitude, spectral responsivity. At same sensor features saturated (100%) quantum efficiency in range 405-1550 nm. Benefiting from multidimensional modulation ultra-high sensitivity, classification accuracy 92.22% three letters achieved only 0.12 photons per pixel pattern. Furthermore, image preprocessing are demonstrated. Photon-efficient beneficial tasks extremely low-light environments covert imaging, biological imaging space exploration. The can be scaled up construct complex neural networks, enabling real-time high sensitivity.

Language: Английский

Citations

0
Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits DOI
Cécile Telliez, Ruth R. Sims, Giulia Faini

et al.

ACS Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Language: Английский

Citations

0
Pupil engineering for extended depth-of-field imaging in a fluorescence miniscope DOI Creative Commons
J. E. Greene, Yujia Xue, Jeffrey Alido

et al.

Neurophotonics, Journal Year: 2023, Volume and Issue: 10(04)

Published: May 8, 2023

Fluorescence head-mounted microscopes, i.e., miniscopes, have emerged as powerful tools to analyze in-vivo neural populations but exhibit a limited depth-of-field (DoF) due the use of high numerical aperture (NA) gradient refractive index (GRIN) objective lenses.We present extended (EDoF) miniscope, which integrates an optimized thin and lightweight binary diffractive optical element (DOE) onto GRIN lens miniscope extend DoF by 2.8× between twin foci in fixed scattering samples.We genetic algorithm that considers lens' aberration intensity loss from Fourier optics-forward model optimize DOE manufacture through single-step photolithography. We integrate into EDoF-Miniscope with lateral accuracy 70 μm produce high-contrast signals without compromising speed, spatial resolution, size, or weight.We characterize performance across 5- 10-μm fluorescent beads embedded phantoms demonstrate facilitates deeper interrogations neuronal 100-μm-thick mouse brain sample vessels whole sample.Built off-the-shelf components augmented customizable DOE, we expect this low-cost may find utility wide range recording applications.

Language: Английский

Citations

8
Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes DOI Creative Commons

Shuzhang Liu,

Jing Ling, Peng R. Chen

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(34)

Published: Aug. 17, 2023

Hybrid voltage indicators (HVIs) are chemogenetic sensors that combines the superior photophysical properties of organic dyes and genetic targetability protein to report transient membrane changes. They exhibit boosted sensitivity in excitable cells such as neurons cardiomyocytes. However, signals recorded during long-term imaging severely diminished or distorted due phototoxicity photobleaching issues. To capture stable electrophysiological activities over a long time, we employ cyanine conjugated with cyclooctatetraene (COT) molecule fluorescence reporter HVI. The resulting orange-emitting HVI–COT–Cy3 enables high-fidelity for up 30 min cultured primary ~ −30% ΔF/F 0 per action potential (AP). It also maximally preserves signal individual APs far-red-emitting HVI–COT–Cy5 allows two-color voltage/calcium GCaMP6s cardiomyocytes 15 min. We leverage HVI–COT series reduced evaluate impact drug candidates on electrophysiology cells.

Language: Английский

Citations

8
GraFT: Graph Filtered Temporal Dictionary Learning for Functional Neural Imaging DOI Creative Commons
Adam S. Charles, Nathan Cermak, Rifqi O. Affan

et al.

IEEE Transactions on Image Processing, Journal Year: 2022, Volume and Issue: 31, P. 3509 - 3524

Published: Jan. 1, 2022

Optical imaging of calcium signals in the brain has enabled researchers to observe activity hundreds-to-thousands individual neurons simultaneously. Current methods predominantly use morphological information, typically focusing on expected shapes cell bodies, better identify field-of-view. The explicit shape constraints limit applicability automated identification other important scales with more complex morphologies, e.g., dendritic or widefield imaging. Specifically, fluorescing components may be broken up, incompletely found, merged ways that do not accurately describe underlying neural activity. Here we present Graph Filtered Temporal Dictionary (GraFT), a new approach frames problem isolating independent as dictionary learning problem. focus time-traces-the main quantity used scientific discovery-and learn time trace spatial maps acting presence coefficients encoding which pixels time-traces are active in. Furthermore, novel graph filtering model redefines connectivity between terms their shared temporal activity, rather than proximity. This greatly eases ability our method handle data non-local structure. We demonstrate properties method, such robustness morphology, simultaneously detecting different neuronal types, and implicitly inferring number neurons, both synthetic real examples. applications at dendritic, somatic, scales.

Language: Английский

Citations

13
Scanless two-photon voltage imaging DOI Creative Commons
Ruth R. Sims, Imane Bendifallah, Christiane Grimm

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 24, 2023

Parallel light-sculpting methods have been used to perform scanless two-photon photostimulation of multiple neurons simultaneously during all-optical neurophysiology experiments. We demonstrate that excitation also enables high-resolution, high-contrast, voltage imaging by efficiently exciting fluorescence in a large fraction the cellular soma. present thorough characterisation using existing parallel approaches and lasers with different repetition rates. recordings high frequency spike trains sub-threshold depolarizations intact brain tissue from expressing soma-targeted genetically encoded indicator JEDI-2P-kv. Using low repetition-rate laser, we up ten simultaneously. Finally, co-expressing JEDI-2P-kv channelrhodopsin ChroME-ST hippocampal organotypic slices, single-beam, simultaneous, photostimulation. This in-situ validation precise number timing light evoked action potentials will pave way for rapid scalable identification functional connections neural circuits.

Language: Английский

Citations

7