Morphological diversity of single neurons in molecularly defined cell types

Nature, Journal Year: 2021, Volume and Issue: 598(7879), P. 174 - 181

Published: Oct. 6, 2021

Abstract Dendritic and axonal morphology reflects the input output of neurons is a defining feature neuronal types 1,2 , yet our knowledge its diversity remains limited. Here, to systematically examine complete single-neuron morphologies on brain-wide scale, we established pipeline encompassing sparse labelling, whole-brain imaging, reconstruction, registration analysis. We fully reconstructed 1,741 from cortex, claustrum, thalamus, striatum other brain regions in mice. identified 11 major projection neuron with distinct morphological features corresponding transcriptomic identities. Extensive projectional was found within each these types, basis which some were clustered into more refined subtypes. This follows set generalizable principles that govern long-range projections at different levels, including molecular correspondence, divergent or convergent projection, axon termination pattern, regional specificity, topography, individual cell variability. Although clear concordance profiles evident level type, fine-grained often does not readily correlate subtypes derived unsupervised clustering, highlighting need for single-cell cross-modality studies. Overall, study demonstrates crucial quantitative description anatomy cell-type classification, as reveals plethora ways their members may contribute configuration function respective circuits.

Language: Английский

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Architectures of neuronal circuits

Science, Journal Year: 2021, Volume and Issue: 373(6559)

Published: Sept. 2, 2021

Although individual neurons are the basic unit of nervous system, they process information by working together in neuronal circuits with specific patterns synaptic connectivity. Here, I review common circuit motifs and architectural plans used diverse brain regions animal species. also consider how these architectures assemble during development might have evolved. Understanding connectivity can implement neural computations will help to bridge huge gap between biology neuron function entire brain, allow us better understand basis behavior, may inspire new advances artificial intelligence.

Language: Английский

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The mouse cortico–basal ganglia–thalamic network

Nature, Journal Year: 2021, Volume and Issue: 598(7879), P. 188 - 194

Published: Oct. 6, 2021

The cortico-basal ganglia-thalamo-cortical loop is one of the fundamental network motifs in brain. Revealing its structural and functional organization critical to understanding cognition, sensorimotor behaviour, natural history many neurological neuropsychiatric disorders. Classically, this conceptualized contain three information channels: motor, limbic associative1-4. Yet three-channel view cannot explain myriad functions basal ganglia. We previously subdivided dorsal striatum into 29 domains on basis topography inputs from entire cortex5. Here we map multi-synaptic output pathways these striatal through globus pallidus external part (GPe), substantia nigra reticular (SNr), thalamic nuclei cortex. Accordingly, identify 14 SNr 36 GPe a direct cortico-SNr projection. striatonigral pathway displays greater convergence than more parallel striatopallidal indirect pathway, although originating same domain ultimately converge onto postsynaptic neurons. Following outputs, delineate six parafascicular ventromedial nuclei. Subsequently, ganglia-thalamic subnetworks that sequentially transduce specific subsets cortical every elemental node cortico-basal loop. Thalamic relay back corticostriatal neurons each subnetwork bona fide closed

Language: Английский

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Wave-like dopamine dynamics as a mechanism for spatiotemporal credit assignment

Cell, Journal Year: 2021, Volume and Issue: 184(10), P. 2733 - 2749.e16

Published: April 16, 2021

Language: Английский

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A midbrain-thalamus-cortex circuit reorganizes cortical dynamics to initiate movement

Cell, Journal Year: 2022, Volume and Issue: 185(6), P. 1065 - 1081.e23

Published: March 1, 2022

Motor behaviors are often planned long before execution but only released after specific sensory events. Planning and each associated with distinct patterns of motor cortex activity. Key questions how these dynamic activity generated they relate to behavior. Here, we investigate the multi-regional neural circuits that link an auditory "Go cue" transition from planning directional licking. Ascending glutamatergic neurons in midbrain reticular pedunculopontine nuclei show short latency phasic changes spike rate selective for Go cue. This signal is transmitted via thalamus cortex, where it triggers a rapid reorganization state planning-related command, which turn drives appropriate movement. Our studies can control cortical dynamics precise

Language: Английский

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Decoding molecular and cellular heterogeneity of mouse nucleus accumbens

Nature Neuroscience, Journal Year: 2021, Volume and Issue: 24(12), P. 1757 - 1771

Published: Oct. 18, 2021

Language: Английский

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Networking brainstem and basal ganglia circuits for movement

Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(6), P. 342 - 360

Published: April 14, 2022

Language: Английский

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Specific populations of basal ganglia output neurons target distinct brain stem areas while collateralizing throughout the diencephalon

Neuron, Journal Year: 2021, Volume and Issue: 109(10), P. 1721 - 1738.e4

Published: April 5, 2021

Basal ganglia play a central role in regulating behavior, but the organization of their outputs to other brain areas is incompletely understood. We investigate largest output nucleus, substantia nigra pars reticulata (SNr), and delineate physiology its projection populations mice. Using genetically targeted viral tracing whole-brain anatomical analysis, we identify over 40 SNr targets that encompass roughly 50-fold range axonal densities. Retrograde from volumetrically indicates contains segregated subpopulations differentially project functionally distinct stem regions. These are electrophysiologically specialized topographically organized collateralize common diencephalon targets, including motor intralaminar thalamus as well pedunculopontine nucleus midbrain reticular formation. findings establish signaling dense, parallel specific concurrent with extensive collateral branches majority boutons.

Language: Английский

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The integrated brain network that controls respiration

eLife, Journal Year: 2023, Volume and Issue: 12

Published: March 8, 2023

Respiration is a brain function on which our lives essentially depend. Control of respiration ensures that the frequency and depth breathing adapt continuously to metabolic needs. In addition, respiratory control network has organize muscular synergies integrate ventilation with posture body movement. Finally, coupled cardiovascular emotion. Here, we argue can handle this all by integrating brainstem central pattern generator circuit in larger also comprises cerebellum. Although currently not generally recognized as center, cerebellum well known for its coordinating modulating role motor behavior, autonomic nervous system. review, discuss regions involved respiration, their anatomical functional interactions. We how sensory feedback result adaptation these mechanisms be compromised various neurological psychological disorders. demonstrate generators are part integrated regions.

Language: Английский

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Opponent control of behavior by dorsomedial striatal pathways depends on task demands and internal state

Nature Neuroscience, Journal Year: 2022, Volume and Issue: 25(3), P. 345 - 357

Published: March 1, 2022

Language: Английский

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