International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(3), P. 2344 - 2344
Published: Jan. 25, 2023
The
native
role
of
extracellular
vesicles
(EVs)
in
mediating
the
transfer
biomolecules
between
cells
has
raised
possibility
to
use
them
as
therapeutic
vehicles.
development
therapies
based
on
EVs
is
now
expanding
rapidly;
here
we
will
describe
current
knowledge
different
key
points
regarding
a
clinical
setting.
These
are
related
cell
sources
EVs,
isolation,
storage,
and
delivery
methods,
well
modifications
releasing
for
improved
production
EVs.
Finally,
depict
application
trials,
considering
impact
COVID-19
pandemic
these
therapies,
pointing
out
that
although
it
promising
therapy
human
diseases,
still
initial
phase
its
patients.
Cancers,
Journal Year:
2023,
Volume and Issue:
15(4), P. 1307 - 1307
Published: Feb. 18, 2023
Extracellular
vesicles
(EVs)
are
secreted
by
all
living
cells
and
ubiquitous
in
every
human
body
fluid.
They
quite
heterogeneous
with
regard
to
biogenesis,
size,
composition,
yet
always
reflect
their
parental
cell-of-origin
specific
cargo
loading.
Since
numerous
studies
have
demonstrated
that
EV-associated
proteins,
nucleic
acids,
lipids,
metabolites
can
represent
malignant
phenotypes
cancer
patients,
EVs
increasingly
being
discussed
as
valuable
carriers
of
biomarkers
liquid
biopsy
samples.
However,
the
lack
standardized
clinically
feasible
protocols
for
EV
purification
characterization
still
limits
applicability
EV-based
biomarker
analysis.
This
review
first
provides
an
overview
current
isolation
techniques
be
used
exploit
patient-derived
fluids
quantification
assays.
Secondly,
it
outlines
promising
tumor-specific
relevant
diagnosis,
disease
monitoring,
prediction
progression
therapy
resistance.
Finally,
we
summarize
advantages
limitations
using
a
prospective
view
on
strategies
ongoing
clinical
implementation
screenings.
Advanced Healthcare Materials,
Journal Year:
2023,
Volume and Issue:
12(28)
Published: July 8, 2023
Extracellular
vesicles
(EVs)
occur
in
a
variety
of
bodily
fluids
and
have
gained
recent
attraction
as
natural
materials
due
to
their
bioactive
surfaces,
internal
cargo,
role
intercellular
communication.
EVs
contain
various
biomolecules,
including
surface
cytoplasmic
proteins;
nucleic
acids
that
are
often
representative
the
originating
cells.
can
transfer
content
other
cells,
process
is
thought
be
important
for
several
biological
processes,
immune
responses,
oncogenesis,
angiogenesis.
An
increased
understanding
underlying
mechanisms
EV
biogenesis,
composition,
function
has
led
an
exponential
increase
preclinical
clinical
assessment
biomedical
applications,
such
diagnostics
drug
delivery.
Bacterium-derived
vaccines
been
use
decades
few
EV-based
diagnostic
assays
regulated
under
Clinical
Laboratory
Improvement
Amendments
approved
single
laboratories.
Though,
products
yet
receive
widespread
approval
from
national
regulatory
agencies
United
States
Food
Drug
Administration
(USFDA)
European
Medicine
Agency
(EMA),
many
late-stage
trials.
This
perspective
sheds
light
on
unique
characteristics
EVs,
highlighting
current
trends,
emerging
challenges
future
perspectives
use.
Pharmacological Reviews,
Journal Year:
2023,
Volume and Issue:
75(5), P. 1043 - 1061
Published: June 6, 2023
Extracellular
vesicles
(EVs)
are
cell-derived
membrane
enclosed
particles
that
involved
in
physiological
and
pathological
processes.
EVs
increasingly
being
studied
for
therapeutic
applications
the
field
of
regenerative
medicine.
Therapeutic
application
stem
has
shown
great
potential
to
stimulate
tissue
repair.
However,
exact
mechanisms
through
which
they
induce
this
effect
not
been
fully
clarified.
This
may
a
large
extent
be
attributed
lack
knowledge
on
EV
heterogeneity.
Recent
studies
suggest
represent
heterogeneous
population
with
distinct
functions.
The
heterogeneity
can
differences
their
biogenesis,
as
such,
classified
into
populations
then
further
subcategorized
various
subpopulations.
A
better
understanding
is
crucial
elucidating
action
regeneration.
review
provides
an
overview
latest
insights
related
repair,
including
different
characteristics
contribute
such
heterogeneity,
well
functional
among
subtypes.
It
also
sheds
light
challenges
hinder
clinical
translation
EVs.
Additionally,
innovative
isolation
techniques
studying
discussed.
Improved
active
subtypes
would
promote
development
tailored
therapies
aid
researchers
EV-based
therapeutics
clinic.
Significance
Statement
Within
we
discuss
properties
subpopulations,
implications
therapeutics.
We
aim
provide
new
aspects
leading
preparations,
stress
importance
applications.
Biomedicine & Pharmacotherapy,
Journal Year:
2023,
Volume and Issue:
165, P. 115087 - 115087
Published: June 29, 2023
All
forms
of
life
produce
nanosized
extracellular
vesicles
called
exosomes,
which
are
enclosed
in
lipid
bilayer
membranes.
Exosomes
engage
cell-to-cell
communication
and
participate
a
variety
physiological
pathological
processes.
function
via
their
bioactive
components,
delivered
to
target
cells
the
form
proteins,
nucleic
acids,
lipids.
as
drug
delivery
vehicles
due
unique
properties
innate
stability,
low
immunogenicity,
biocompatibility,
biodistribution,
accumulation
desired
tissues,
toxicity
normal
stimulation
anti-cancer
immune
responses,
penetration
capacity
into
distance
organs.
mediate
cellular
communications
by
delivering
various
molecules
including
oncogenes,
oncomiRs,
specific
DNA,
messenger
RNA
(mRNA),
microRNA
(miRNA),
small
interfering
(siRNA),
circular
(circRNA).
These
substances
can
be
transferred
change
transcriptome
influence
tumor-related
signaling
pathways.
After
considering
all
available
literature,
this
review
we
discuss
biogenesis,
composition,
production,
purification
exosomes.
We
briefly
exosome
isolation
techniques.
explore
great-length
exosomes
mechanism
for
substances,
chemicals,
chemotherapeutic
drugs.
also
talk
about
benefits
drawbacks
This
concludes
with
discussion
future
perspective
challenges.
hope
that
will
provide
us
better
understanding
current
state
nanomedicine
applications
biomedicine.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(3), P. 2344 - 2344
Published: Jan. 25, 2023
The
native
role
of
extracellular
vesicles
(EVs)
in
mediating
the
transfer
biomolecules
between
cells
has
raised
possibility
to
use
them
as
therapeutic
vehicles.
development
therapies
based
on
EVs
is
now
expanding
rapidly;
here
we
will
describe
current
knowledge
different
key
points
regarding
a
clinical
setting.
These
are
related
cell
sources
EVs,
isolation,
storage,
and
delivery
methods,
well
modifications
releasing
for
improved
production
EVs.
Finally,
depict
application
trials,
considering
impact
COVID-19
pandemic
these
therapies,
pointing
out
that
although
it
promising
therapy
human
diseases,
still
initial
phase
its
patients.
