International review of cell and molecular biology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Journal of Extracellular Vesicles, Journal Year: 2022, Volume and Issue: 11(6)
Published: June 1, 2022
Abstract Extracellular vesicles (EVs) play a key role in many physiological and pathophysiological processes hold great potential for therapeutic diagnostic use. Despite significant advances within the last decade, issue of EV storage stability remains unresolved under investigated. Here, we aimed to identify conditions stabilizing EVs comprehensively compared impact various buffer formulations at different temperatures on derived from cellular sources up 2 years. features including concentration, diameter, surface protein profile nucleic acid contents were assessed by complementary methods, engineered containing fluorophores or functionalized proteins utilized compare uptake ligand binding. We show that storing PBS over time leads drastically reduced recovery particularly pure samples all tested, starting already days. further report using as diluent was found result severely rates minutes. Several tested new largely prevented observed effects, lead candidate being supplemented with human albumin trehalose (PBS‐HAT). PBS‐HAT facilitates clearly improved short‐term long‐term preservation stored ‐80°C, throughout several freeze‐thaw cycles, when downstream applications.
Language: Английский
Citations
242
Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(6), P. 709 - 709
Published: May 24, 2024
Of all the numerous nanosized extracellular vesicles released by a cell, endosomal-originated exosomes are increasingly recognized as potential therapeutics, owing to their inherent stability, low immunogenicity, and targeted delivery capabilities. This review critically evaluates transformative of exosome-based modalities across pharmaceutical precision medicine landscapes. Because precise biomolecular cargo delivery, posited ideal candidates in drug enhancing regenerative strategies, advancing diagnostic technologies. Despite significant market growth projections exosome therapy, its utilization is encumbered substantial scientific regulatory challenges. These include lack universally accepted protocols for isolation complexities associated with navigating environment, particularly guidelines set forth U.S. Food Drug Administration (FDA). presents comprehensive overview current research trajectories aimed at addressing these impediments discusses prospective advancements that could substantiate clinical translation exosomal therapies. By providing analysis both capabilities hurdles therapeutic applications, this article aims inform direct future paradigms, thereby fostering integration systems into mainstream practice.
Language: Английский
Citations
17
Theranostics, Journal Year: 2022, Volume and Issue: 12(15), P. 6455 - 6508
Published: Jan. 1, 2022
Small extracellular vesicles (sEVs) have been proposed as a possible solution to the current lack of therapeutic interventions for endogenous skin regeneration. We conducted systematic review available evidence assess sEV efficacy and safety in wound healing regeneration animal models. 68 studies were identified Web Science, Scopus, PubMed that satisfied set prespecified inclusion criteria. critically analyzed quality our criteria, with an emphasis on methodology, reporting, adherence relevant guidelines (including MISEV2018 ISCT criteria). Overall, meta-analysis indicated promoted diabetic non-diabetic models influenced various facets process regardless cell source, production protocol disease model. The EV isolation methods, dosing regimen, size varied among studies. Modification sEVs was achieved mainly by manipulating source cells via preconditioning, nanoparticle loading, genetic manipulation, biomaterial incorporation enhance potential. Evaluation potential adverse effects received only minimal attention, although none reported harmful events. Risk bias assessed SYRCLE's ROB tool uncertain most due insufficient limited. In summary, therapy has enormous However, reproducibility comprehensive evaluation are challenged general transparency reporting guidelines. Methodological rigor, standardization, risk analysis at all stages research needed promote translation clinical practice.
Language: Английский
Citations
53
Small, Journal Year: 2022, Volume and Issue: 18(36)
Published: March 23, 2022
The unique biological characteristics and promising clinical potential of extracellular vesicles (EVs) have galvanized EV applications for regenerative medicine. Recognized as important mediators intercellular communication, naturally secreted EVs the potential, innate biotherapeutics, to promote tissue regeneration. Although emerged novel therapeutic agents, challenges related transition led further functionalization. In recent years, various engineering approaches such preconditioning, drug loading, surface modification been developed potentiate outcomes EVs. Also, limitations natural addressed by development artificial that offer advantages in terms production yield isolation methodologies. this review, an updated overview current techniques is provided functionalization advances EVs, particularly scope
Language: Английский
Citations
52
Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)
Published: May 10, 2022
Abstract Brain metastasis (BrM) is a major problem associated with cancer-related mortality, and currently, no specific biomarkers are available in clinical settings for early detection. Liquid biopsy widely accepted as non-invasive method diagnosing cancer other diseases. We have reviewed the evidence that shows how molecular alterations involved BrM, majorly from breast (BC), lung (LC), melanoma, an inception they can be employed biomarker development. discussed genetic epigenetic changes influence cells to breach blood-brain barrier (BBB) help establish metastatic lesions uniquely distinct brain microenvironment. Keeping abreast recent breakthroughs context of various biomolecules detections identifications, circulating tumor (CTC), cell-free nucleotides, non-coding RNAs, secretory proteins, metabolites pursued human body fluids such blood, serum, cerebrospinal fluid (CSF), urine obtain potential candidates The liquid biopsy-based overlay current imaging techniques amplify signal viable improving detection treatments occult BrM.
Language: Английский
Citations
45
iScience, Journal Year: 2022, Volume and Issue: 25(4), P. 104012 - 104012
Published: March 1, 2022
Extracellular vesicles (EVs) are ubiquitously secreted by almost all tissues and carry many cargoes, including proteins, RNAs, lipids, which related to various biological processes. EVs shed from into the blood expected be used as biomarkers for diseases. Here, we isolated EDTA plasma serum of six healthy subjects an affinity capture isolation method, a total 4,079 proteins were successfully identified comprehensive EV proteomics. Our reliable detailed catalog differential expression profiles in between individuals could useful reference biomarker discovery. Furthermore, tissue-specific protein groups co-regulated identified. These more specific sensitive enrichment screening monitoring disease without diagnostic imaging patient future.
Language: Английский
Citations
44
Journal of Tissue Engineering, Journal Year: 2023, Volume and Issue: 14, P. 204173142311746 - 204173142311746
Published: Jan. 1, 2023
While extracellular vesicles (EVs) continue to gain interest for therapeutic applications, their clinical translation is limited by a lack of optimal isolation methods. We sought determine how universally applied methods impact EV purity and yield. EVs were isolated ultracentrifugation (UC), polyethylene glycol precipitation, Total Exosome Isolation Reagent, an aqueous two-phase system with without repeat washes or size exclusion chromatography (SEC). EV-like particles could be detected all but varied in relative expression surface markers (Alix, Annexin A2, CD9, CD63 CD81). Assessments sample dependent on the specificity characterisation method applied, total particle counts protein (PtP) ratios often not aligning quantitative measures tetraspanin obtained using high-resolution nano-flow cytometry. SEC resulted fewer relatively low PtP ratio (1.12 × 10 7 ± 1.43 6 vs highest recorded; ATPS/R 2.01 8 1.15 9 , p ⩽ 0.05), this displayed comparatively high level positivity (e.g. ExoELISA CD63⁺ particles; 1.36 11 1.18 2.58 1.92 0.001). Results originating from accompanying survey designed evaluate pragmatic considerations surrounding implementation scalability cost) identified that UC favoured overall efficiency. However, reservations highlighted these methods, which potentially hinder downstream applications. In conclusion, variations yield evident between while standard non-specific assessments did align advanced analysis markers. Reproducible specific will critical informing studies.
Language: Английский
Citations
29
Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 27 - 27
Published: Jan. 1, 2025
Exosomes are small extracellular vesicles and crucial in intercellular communication. Interestingly, tumor-derived exosomes carry oncogenic molecules, such as proteins microRNAs, which can reprogram recipient cells, promote angiogenesis, stimulate cancer pre-metastatic niche, supporting growth metastasis. On the other hand, their biocompatibility, stability, ability to cross biological barriers make them attractive candidates for drug delivery. Recent advances have shown potential be used early disease detection targeted therapy by delivering therapeutic agents specifically tumor sites. Despite promising applications, a number of challenges remain, including exosome isolation characterization, well inherent heterogeneity. Thus, current review aims describe roles health disease, discuss that hinder development into becoming useful medical tools.
Language: Английский
Citations
1
Advanced Healthcare Materials, Journal Year: 2022, Volume and Issue: 12(8)
Published: Dec. 21, 2022
Abstract Extracellular vesicles (EVs) are nanoscale membrane vesicles, which contain a wide variety of cargo such as proteins, miRNAs, and lipids. A growing body evidence suggests that EVs promising biomarkers for disease diagnosis therapeutic strategies. Although the excellent clinical value, their use in personalized healthcare practice is not yet feasible due to highly heterogeneous nature. Taking difficulty isolation small size into account, characterization at single‐particle level both imperative challenging. In bid address this critical point, more research has been directed microfluidic platform because its inherent advantages sensitivity, specificity, throughput. This review discusses biogenesis heterogeneity takes broad view state‐of‐the‐art advances microfluidics‐based EV research, including only separation, but also single biophysical detection biochemical analysis. To highlight techniques, conventional technologies included comparison. The current status artificial intelligence (AI) then presented. Furthermore, challenges prospects microfluidics combination with AI applications discussed. foreseeable future, recent breakthroughs platforms expected pave way analysis improve precision medicine.
Language: Английский
Citations
30
Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(11), P. 2623 - 2623
Published: Nov. 14, 2023
Biomimetic delivery systems (BDSs), inspired by the intricate designs of biological systems, have emerged as a groundbreaking paradigm in nanomedicine, offering unparalleled advantages therapeutic delivery. These encompassing platforms such liposomes, protein-based nanoparticles, extracellular vesicles, and polysaccharides, are lauded for their targeted delivery, minimized side effects, enhanced outcomes. However, translation BDSs from research settings to clinical applications is fraught with challenges, including reproducibility concerns, physiological stability, rigorous efficacy safety evaluations. Furthermore, innovative nature demands reevaluation evolution existing regulatory ethical frameworks. This review provides an overview delves into multifaceted translational challenges present emerging solutions, underscored real-world case studies. Emphasizing potential redefine healthcare, we advocate sustained interdisciplinary collaboration research. As our understanding deepens, future appears promising, focus on personalized medicine refined patient-specific systems.
Language: Английский
Citations
20