Regenerative Engineering and Translational Medicine, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 17, 2025
Language: Английский
Military Medical Research, Journal Year: 2023, Volume and Issue: 10(1)
Published: Aug. 17, 2023
Abstract Skin wounds are characterized by injury to the skin due trauma, tearing, cuts, or contusions. As such injuries common all human groups, they may at times represent a serious socioeconomic burden. Currently, increasing numbers of studies have focused on role mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in wound repair. cell-free therapy, MSC-derived EVs shown significant application potential field repair as more stable and safer option than conventional therapy. Treatment based can significantly promote damaged substructures, including regeneration vessels, nerves, hair follicles. In addition, inhibit scar formation affecting angiogenesis-related antifibrotic pathways promoting macrophage polarization, angiogenesis, proliferation, migration, inhibiting excessive matrix production. Additionally, these structures serve scaffold for components used repair, be developed into bioengineered support trauma Through formulation standardized culture, isolation, purification, drug delivery strategies, exploration detailed mechanism will allow them clinical treatments conclusion, EVs-based therapies important prospects Here we provide comprehensive overview their current status, potential, associated drawbacks.
Language: Английский
Citations
92
Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)
Published: Oct. 1, 2022
Language: Английский
Citations
75
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130728 - 130728
Published: March 11, 2024
Exosomes (EXOs), minute membranous structures originating from diverse biological sources, have recently seized the attention of researchers due to their theranostic potential for neurological diseases. Released actively by various cells, including stem adipose tissue, and immune EXOs wield substantial regulatory influence over intricate landscape complications, exhibiting both positive negative modulatory effects. In AD, play a pivotal role in disseminating breaking down amyloid-β protein. Moreover, derived mesenchymal cells showcase remarkable capacity mitigate pro-inflammatory phenotypes regulating miRNAs neurodegenerative These vesicles possess unique ability traverse blood-brain barrier, governing aggregation mutant huntingtin Understanding exosomal functions within CNS holds significant promise enhancing treatment efficacy This review intricately examines mechanisms involving disease development, highlighting therapeutic prospects exploring utility exosome-based nanomedicine complications. Additionally, delves into challenges associated with drug delivery brain, emphasizing complexities inherent this critical aspect neurotherapeutics.
Language: Английский
Citations
32
Med, Journal Year: 2024, Volume and Issue: 5(2), P. 148 - 168.e8
Published: Feb. 1, 2024
BackgroundAdipose tissue-derived stem cell-derived apoptotic bodies (ADSC-ABs) have shown great potential for immunomodulation and regeneration, particularly in diabetic wound therapy. However, their local application has been limited by unclear regulatory mechanisms, rapid clearance, short tissue retention times.MethodsWe analyzed the key role molecules pathways of ADSC-ABs regulating inflammatory macrophages mRNA sequencing microRNA (miRNA) then verified them gene knockdown. To prevent we employed microfluidics technology to prepare methacrylate-anhydride gelatin (GelMA) microspheres (GMS) controlled release ABs. Finally, evaluated effectiveness ADSC-AB-laden GMSs (ABs@GMSs) a rat model.FindingsOur results demonstrated that effectively balanced macrophage polarization through janus kinase (JAK)-signal transducer activator transcription (STAT) pathway, mediated miR-20a-5p. Furthermore, showed AB@GMSs had good biocompatibility, significantly delayed clearance ABs, ameliorated inflammation promoted vascularization, thus facilitating its healing.ConclusionsOur study reveals mechanism balancing highlightsthe importance delaying GMSs. These findings important implications development novel therapies healing.FundingThis research was supported National Key Research Development Program China (2020YFA0908200), Natural Science Foundation (82272263, 82002053, 32000937, 82202467), Shanghai "Rising Stars Medical Talents" Youth (22MC1940300), Municipal Health Commission (20204Y0354), Technology Funds (22YF1421400).
Language: Английский
Citations
16
International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 3177 - 3210
Published: June 1, 2023
Abstract: Exosomes are nanovesicles with a wide range of chemical compositions used in many different applications. Mesenchymal stem cell-derived exosomes (MSCs-EXOs) spherical vesicles that have been shown to mediate tissue regeneration variety diseases, including neurological, autoimmune and inflammatory, cancer, ischemic heart disease, lung injury, liver fibrosis. They can modulate the immune response by interacting effector cells due presence anti-inflammatory compounds involved intercellular communication through various types cargo. MSCs-EXOs exhibit cytokine storm-mitigating properties COVID-19. This review discussed potential function diseases notably epileptic encephalopathy Parkinson's angiogenesis, inflammatory diseases. We provided an overview exosome biogenesis factors regulate biogenesis. Additionally, we highlight functions use treatment disease Finally, covered strategies challenges MSCs-EXOs. discuss conclusion future perspectives Keywords: extracellular vesicle, biogenesis, mesenchymal cells, exosomes, angiogenesis
Language: Английский
Citations
39
Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)
Published: Sept. 19, 2023
Abstract Peripheral nerve injury (PNI) is one of the public health concerns that can result in a loss sensory or motor function areas which injured and non-injured nerves come together. Up until now, there has been no optimized therapy for complete regeneration after PNI. Exosome-based therapies are an emerging effective therapeutic strategy promoting functional recovery. Exosomes, as natural extracellular vesicles, contain bioactive molecules intracellular communications nervous tissue function, could overcome challenges cell-based therapies. Furthermore, bioactivity ability exosomes to deliver various types agents, such proteins microRNA, have made potential approach neurotherapeutics. However, type cell origin, dosage, targeted delivery still pose clinical translation exosome therapeutics. In this review, we focused on Schwann mesenchymal stem (MSC)-derived regeneration. Also, expressed current understanding MSC-derived related provided insights developing cell-free MSC injury.
Language: Английский
Citations
29
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: Aug. 25, 2023
Wound repair is a complex problem for both clinical practitioners and scientific investigators. Conventional approaches to wound have been associated with several limitations, including prolonged treatment duration, high expenses, significant economic psychological strain on patients. Consequently, there pressing demand more efficacious secure modalities enhance the existing landscapes. In field of repair, cell-free therapy, particularly use mesenchymal stem cell-derived exosomes (MSC-Exos), has made notable advancements in recent years. Exosomes, which are small lipid bilayer vesicles discharged by MSCs, harbor bioactive constituents such as proteins, lipids, microRNA (miRNA), messenger RNA (mRNA). These facilitate material transfer information exchange between cells, thereby regulating their biological functions. This article presents comprehensive survey function mechanisms MSC-Exos context healing, emphasizing beneficial impact each phase process, regulation immune response, inhibition inflammation, promotion angiogenesis, advancement cell proliferation migration, reduction scar formation.
Language: Английский
Citations
27
Biomolecules, Journal Year: 2024, Volume and Issue: 14(4), P. 459 - 459
Published: April 9, 2024
The skin is the outer layer of human body, and it crucial in defending against injuries damage. regenerative capacity aging damaged caused by exposure to external stimuli significantly impaired. Currently, rise average life expectancy modern population’s aesthetic standards have sparked a desire for stem-cell-based therapies that can address health conditions. In recent years, mesenchymal stem cells (MSCs) as therapeutic agents provided promising effective alternative managing regeneration rejuvenation, attributing their healing capacities be applied aged skin. However, has been established effects MSC may primarily mediated paracrine mechanisms, particularly release exosomes (Exos). Exosomes are nanoscale extracellular vesicles (EVs) lipid bilayer membrane structures naturally released different types cells. They influence physiological pathological processes recipient transferring variety bioactive molecules, including lipids, proteins, nucleic acids such messenger RNAs (mRNAs) microRNAs (miRNAs) between cells, thus playing an important role intercellular communication activating signaling pathways target Among them, miRNAs, type endogenous regulatory non-coding RNA, often incorporated into molecules regulating protein biosynthesis. Emerging evidence suggests exosomal miRNAs from play key rejuvenation targeting multiple genes various biological processes, participating inflammatory responses, cell migration, proliferation, apoptosis. this review, we summarize studies observations on how MSC-derived contribute tissue, with particular attention applications bioengineering methods manipulating miRNA content exosome cargo improve potential. This review provide new clues diagnosis treatment damage aging, well assist investigators exploring innovative strategies treating multitude problems aim delaying promoting regeneration, maintaining healthy
Language: Английский
Citations
10
Regenerative Therapy, Journal Year: 2024, Volume and Issue: 26, P. 260 - 274
Published: June 1, 2024
Chronic wounds represent a significant global burden, afflicting millions with debilitating complications. Despite standard care, impaired healing persists due to factors like persistent inflammation and tissue regeneration. Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) offer an innovative regenerative medicine approach, delivering cell-derived therapeutic cargo in engineered nanoscale delivery systems. This review examines pioneering bioengineering strategies engineer MSC-EVs into precision nanotherapeutics for chronic wounds. Emerging technologies CRISPR gene editing, microfluidic manufacturing, biomimetic systems are highlighted their potential enhance MSC-EV targeting, optimize enrichment, ensure consistent clinical-grade production. However, key hurdles remain, including batch variability, rigorous safety assessment tumorigenicity, immunogenicity, biodistribution profiling. Crucially, collaborative frameworks harmonizing regulatory science patient advocacy hold the expediting clinical translation. By overcoming these challenges, could catalyze new era of off-the-shelf therapies, restoring hope afflicted by non-healing
Language: Английский
Citations
10
Biomedicines, Journal Year: 2024, Volume and Issue: 12(4), P. 805 - 805
Published: April 4, 2024
Pain is the major symptom of osteoarthritis (OA) and an important factor in strategies to manage this disease. However, current standard care does not provide satisfactory pain relief for many patients. The pathophysiology OA complex, its presentation as a clinical syndrome associated with pathologies multiple joint tissues. Treatment options are generally classified pharmacologic, nonpharmacologic, surgical, complementary and/or alternative, typically used combination achieve optimal results. goals treatment alleviation symptoms improvement functional status. Several studies exploring various directions management, including tissue regeneration techniques, personalized medicine, targeted drug therapies. aim present narrative review extensively describe all treatments available practice, further describing most innovative Advancements understanding molecular genetic aspects may lead more effective tailored approaches future.
Language: Английский
Citations
9