Evolutionary History of Chemosensory-Related Gene Families across the Arthropoda

Molecular Biology and Evolution, Journal Year: 2017, Volume and Issue: 34(8), P. 1838 - 1862

Published: April 27, 2017

Chemosensory-related gene (CRG) families have been studied extensively in insects, but their evolutionary history across the Arthropoda had remained relatively unexplored. Here, we address current hypotheses and prior conclusions on CRG family evolution using a more comprehensive data set. In particular, odorant receptors were hypothesized to proliferated during terrestrial colonization by insects (hexapods), association with other pancrustacean clades independent colonizations arthropod subphyla unclear. We also examine which is most ancient. Thus, reconstructed phylogenies of CRGs, including those from new genomes transcriptomes, mapped gains losses lineages. Our analysis was strengthened crustaceans, especially copepods, reside outside hexapod/branchiopod clade within subphylum Pancrustacea. generated first high-resolution genome sequence copepod Eurytemora affinis annotated its CRGs. found binding proteins present only hexapods (insects) absent all lineages, indicating that they are not universal adaptations land. Gustatory likely represent oldest chemosensory among dating back Placozoa. clarified confirmed antennal ionotropic Arthropoda. All E. expressed highly males than females, suggestive an male mate-recognition behavior. This study comparative date largest speciose metazoan phylum

Language: Английский

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An expression atlas of variant ionotropic glutamate receptors identifies a molecular basis of carbonation sensing

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Oct. 8, 2018

Abstract Through analysis of the Drosophila ionotropic receptors (IRs), a family variant glutamate receptors, we reveal that most IRs are expressed in peripheral neuron populations diverse gustatory organs larvae and adults. We characterise IR56d, which defines two anatomically-distinct classes proboscis: one responds to carbonated solutions fatty acids while other represents subset sugar- acid-sensing cells. Mutational indicates together with broadly-expressed co-receptors IR25a IR76b, is essential for physiological responses carbonation acids, but not sugars. further demonstrate both promote IR56d-dependent attraction flies, through different behavioural outputs. Our work provides toolkit investigating taste functions IRs, these required sensing, illustrates how system uses combinatorial expression sensory molecules distinct neurons coordinate behaviour.

Language: Английский

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Functional architecture of reward learning in mushroom body extrinsic neurons of larval Drosophila

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: March 12, 2018

The brain adaptively integrates present sensory input, past experience, and options for future action. insect mushroom body exemplifies how a central structure brings about such integration. Here we use combination of systematic single-cell labeling, connectomics, transgenic silencing, activation experiments to study the at resolution, focusing on behavioral architecture its input output neurons (MBINs MBONs), intrinsic APL neuron. Our results reveal identity morphology almost all these 44 in stage 3 Drosophila larvae. Upon an initial screen, functional analyses medial lobe uncover sparse specific functions dopaminergic MBINs, MBONs, GABAergic neuron across three tasks, namely odor preference, taste associative learning between taste. thus provide cellular-resolution case brains organize behavior.

Language: Английский

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Ionotropic Receptors Mediate Drosophila Oviposition Preference through Sour Gustatory Receptor Neurons

Current Biology, Journal Year: 2017, Volume and Issue: 27(18), P. 2741 - 2750.e4

Published: Sept. 1, 2017

Language: Английский

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Molecular basis of fatty acid taste in Drosophila

eLife, Journal Year: 2017, Volume and Issue: 6

Published: Dec. 12, 2017

Behavioral studies have established that Drosophila appetitive taste responses towards fatty acids are mediated by sweet sensing Gustatory Receptor Neurons (GRNs). Here we show GRN activation requires the function of Ionotropic genes IR25a, IR76b and IR56d. The former two IR expressed in several neurons per sensillum, while IR56d expression is restricted to GRNs. Importantly, loss behavioral IR25a mutant flies can be completely rescued respective transgenes Interestingly, wild type hexanoic acid reach a plateau at ~1%, but decrease with higher concentration, property through IR25a/IR76b independent bitter With our previous report on sour taste, suggest IR-based receptors mediate different qualities cell-type specific subunits.

Language: Английский

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Calcium Taste Avoidance in Drosophila

Neuron, Journal Year: 2017, Volume and Issue: 97(1), P. 67 - 74.e4

Published: Dec. 21, 2017

Language: Английский

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The multidimensional ionotropic receptors ofDrosophila melanogaster

Insect Molecular Biology, Journal Year: 2017, Volume and Issue: 27(1), P. 1 - 7

Published: Aug. 31, 2017

Abstract Ionotropic receptors (IRs), which form ion channels, can be categorized into conserved ‘antennal IRs’, define the first olfactory receptor family of insects, and species‐specific ‘divergent are expressed in gustatory neurones. These located primarily cell bodies dendrites, highly enriched tips dendritic terminals that convey sensory information to higher brain centres. Antennal IRs play important roles odour thermosensation, whereas divergent involved other biological processes such as taste sensation. Some known specific perception various molecules; however, many their functions have not yet been defined. Although progress has made this field, mechanisms these remain unknown. In review, we provide a comprehensive summary current state knowledge field.

Language: Английский

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Recent advances in the genetic basis of taste detection in Drosophila

Cellular and Molecular Life Sciences, Journal Year: 2019, Volume and Issue: 77(6), P. 1087 - 1101

Published: Oct. 9, 2019

Language: Английский

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Drosophilasensory receptors—a set of molecular Swiss Army Knives

Genetics, Journal Year: 2021, Volume and Issue: 217(1), P. 1 - 34

Published: Jan. 1, 2021

Genetic approaches in the fruit fly, Drosophila melanogaster, have led to a major triumph field of sensory biology-the discovery multiple large families receptors and channels. Some these families, such as transient receptor potential channels, are conserved from animals ranging worms humans, while others, "gustatory receptors," "olfactory "ionotropic restricted invertebrates. Prior identification flies, it was widely assumed that proteins function just one modality vision, smell, taste, hearing, somatosensation, which includes thermosensation, light, noxious mechanical touch. By employing vast combination genetic, behavioral, electrophysiological, other concept emerge is many multitaskers. The earliest example this idea individual channels senses. It now clear multitasking exhibited by including gustatory receptors, ionotropic epithelial Na+ (also referred Pickpockets), even opsins, were formerly thought exclusively light sensors. characterizations neurons express them also reveal mechanisms through flies can accurately differentiate between different stimuli when they activate same receptor, well adaptation, amplification, integration. insights gleaned studies been highly influential directing investigations animal models.

Language: Английский

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A post-ingestive amino acid sensor promotes food consumption in Drosophila

Cell Research, Journal Year: 2018, Volume and Issue: 28(10), P. 1013 - 1025

Published: Sept. 12, 2018

Adequate protein intake is crucial for the survival and well-being of animals. How animals assess prospective sources ensure dietary amino acid plays a critical role in homeostasis. By using quantitative feeding assay, we show that three acids, L-glutamate (L-Glu), L-alanine (L-Ala) L-aspartate (L-Asp), but not their D-enantiomers or other 17 natural L-amino acids combined, rapidly promote food consumption fruit fly Drosophila melanogaster. This feeding-promoting effect independent mating experience internal nutritional status. In vivo ex calcium imagings six brain neurons expressing diuretic hormone 44 (DH44) can be directly activated by these suggesting are an sensor. Genetic inactivation DH44+ abolishes increase induced whereas genetic activation sufficient to feeding, mediate consumption. Single-cell transcriptome analysis immunostaining reveal putative transporter, CG13248, enriched neurons. Knocking down CG13248 expression blocks eliminates responses acids. Therefore, data identify neuron as key sensor detect enhance via transporter CG13248. These results shed light on regulation homeostasis at organismal levels nervous system.

Language: Английский

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