Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
25(2), P. 1310 - 1318
Published: Jan. 24, 2024
Although
the
formation
of
peptide
assemblies
catalyzed
by
alkaline
phosphatase
(ALP)
has
received
increasing
attention
in
inhibiting
cancer
cells,
detailed
enzyme
kinetics
dephosphorylation
corresponding
phosphopeptide
have
yet
to
be
determined.
We
recently
discovered
that
from
a
phosphopentapeptide
can
form
intracellular
nanoribbons
kill
induced
pluripotent
stem
cells
or
osteosarcoma
but
enzymatic
remain
unknown.
Thus,
we
chose
examine
[NBD-LLLLpY
(1)]
concentrations
below
above
its
critical
micelle
concentration
(CMC).
Our
results
show
exhibits
CMC
75
μM
phosphate
saline
buffer,
and
apparent
Vmax
Km
values
are
approximately
0.24
μM/s
5.67
mM,
respectively.
Despite
remaining
incomplete
at
60
min
all
tested,
200
mainly
nanoribbons,
largely
produces
nanofibers,
generates
nanoparticles.
Moreover,
correlates
with
intranuclear
accumulation
pentapeptide.
By
providing
first
examination
assemblies,
this
work
further
supports
notion
phosphopentapeptides
act
as
new
functional
entity
for
controlling
cell
fates.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(34)
Published: June 5, 2024
Although
self-assembly
has
emerged
as
an
effective
tool
for
fabricating
biomaterials,
achieving
precise
control
over
the
morphologies
and
functionalities
of
resultant
assemblies
remains
ongoing
challenge.
Inspired
by
copper
peptide
naturally
present
in
human
plasma,
this
study,
we
designed
a
synthetic
precursor,
FcGH.
FcGH
can
self-assemble
via
two
distinct
pathways:
spontaneous
Cu
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 8, 2025
The
self-assembly
of
molecules
into
highly
ordered
architectures
is
a
ubiquitous
and
natural
process,
wherein
spontaneously
organize
large
structures
to
perform
diverse
functions.
Drawing
inspiration
from
the
formation
nanostructures,
cell-mediated
has
been
developed
create
functional
assemblies
both
inside
outside
living
cells.
These
techniques
have
employed
regulate
cellular
world
by
leveraging
dynamic
intracellular
extracellular
microenvironment.
This
review
highlights
recent
advances
future
trends
in
self-assembly,
ranging
their
cytocompatible
monomer
designs,
synthetic
strategies,
morphological
control
applications.
assembly
behaviors
are
also
discussed
based
on
dimensionality
self-assembled
morphologies
zero
three
dimensions.
Finally,
this
explores
its
promising
potential
for
biomedical
applications,
clarifying
relationship
between
initial
regulation
therapeutic
effects
subsequent
artificial
assemblies.
Through
rationally
designing
molecular
precisely
controlling
morphologies,
cell
mediated
would
provide
an
innovative
platform
executing
biological
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(29), P. 19038 - 19053
Published: July 9, 2024
Surgical
intervention
is
the
most
common
first-line
treatment
for
severe
traumatic
brain
injuries
(TBIs)
associated
with
high
intracranial
pressure,
while
complexity
of
these
surgical
procedures
often
results
in
complications.
Surgeons
struggle
to
comprehensively
evaluate
TBI
status,
making
it
difficult
select
optimal
strategy.
Here,
we
introduce
a
fluorescence
imaging-based
technology
that
uses
high-quality
silver
indium
selenide-based
quantum
dots
(QDs)
integrated
diagnosis
and
guidance.
These
engineered,
poly(ethylene
glycol)-capped
QDs
emit
near-infrared
region,
are
resistant
phagocytosis,
importantly,
ultrastable
after
epitaxial
growth
an
aluminum-doped
zinc
sulfide
shell
aqueous
phase
renders
long-term
light
irradiation
complex
physiological
environments.
We
found
intravenous
injection
enabled
both
precise
mouse
model
and,
more
comprehensive
evaluation
status
before,
during,
operation
distinguish
from
superficial
hemorrhages,
provide
real-time
monitoring
secondary
hemorrhage,
guide
decision
on
evacuation
hematomas.
This
QD-based
diagnostic
system
could
ultimately
complement
existing
clinical
tools
treating
TBI,
which
may
help
surgeons
improve
patient
outcomes
avoid
unnecessary
procedures.
Nano Letters,
Journal Year:
2024,
Volume and Issue:
24(24), P. 7397 - 7407
Published: June 5, 2024
Cancer
immunotherapies
based
on
cytotoxic
CD8+
T
lymphocytes
(CTLs)
are
highly
promising
for
cancer
treatment.
The
specific
interaction
between
T-cell
receptors
and
peptide-MHC-I
complexes
(pMHC-I)
cell
membranes
critically
determines
their
therapeutic
outcomes.
However,
the
lack
of
appropriate
endogenous
antigens
MHC-I
presentation
disables
tumor
recognition
by
CTLs.
By
devising
three
antigen-loaded
self-assembling
peptides
pY-K(Ag)-ERGD,
pY-K(Ag)-E,
Y-K(Ag)-ERGD
to
noncovalently
generate
light-activatable
supramolecular
at
sites
in
different
manners,
we
report
pY-K(Ag)-ERGD
as
a
candidate
endow
cells
with
pMHC-I
targets
demand.
Specifically,
first
generates
low-antigenic
membranes,
successive
light
pulse
allows
antigen
payloads
efficiently
release
from
scaffold,
directly
producing
antigenic
pMHC-I.
Intravenous
administration
enables
light-controlled
inhibition
when
combined
adoptively
transferred
antigen-specific
Our
strategy
is
feasible
broadening
repertoires
advancing
precision-controlled
immunotherapies.
Nanoscale,
Journal Year:
2023,
Volume and Issue:
15(48), P. 19486 - 19492
Published: Jan. 1, 2023
Through
the
innovative
use
of
surface-displayed
horseradish
peroxidase,
this
work
explores
enzymatic
catalysis
both
bioRAFT
polymerization
and
bioATRP
to
prompt
polymer
synthesis
on
surface
Saccharomyces
cerevisiae
cells,
with
outperforming
polymerization.
The
resulting
modification
living
yeast
cells
synthetic
polymers
allows
for
a
significant
change
in
phenotype,
including
growth
profile,
aggregation
characteristics,
conjugation
non-native
enzymes
clickable
cell
surface,
opening
new
avenues
bioorthogonal
cell-surface
engineering.
