Probiotic‐Inspired Nanomedicine Restores Intestinal Homeostasis in Colitis by Regulating Redox Balance, Immune Responses, and the Gut Microbiome

Advanced Materials, Journal Year: 2022, Volume and Issue: 35(3)

Published: Nov. 7, 2022

Microbiota-based therapeutics offer innovative strategies to treat inflammatory bowel diseases (IBDs). However, the poor clinical outcome so far and limited flexibility of bacterial approach call for improvement. Inspired by health benefits probiotics in alleviating symptoms diseases, bioartificial are designed restore intestinal microenvironment colitis regulating redox balance, immune responses, gut microbiome. The probiotic comprises two components: an E. coli Nissle 1917-derived membrane (EM) as surface biodegradable diselenide-bridged mesoporous silica nanoparticles (SeM) core. When orally administered, probiotic-inspired nanomedicine (SeM@EM) adheres strongly mucus layer restored balance regulation homeostasis a murine model acute induced dextran sodium sulfate. In addition, respective properties EM SeM synergistically alter microbiome favorable state increasing diversity shifting profile anti-inflammatory phenotype. This work suggests safe effective that can IBDs therapy.

Language: Английский

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Advances in the colon-targeted chitosan based multiunit drug delivery systems for the treatment of inflammatory bowel disease

Carbohydrate Polymers, Journal Year: 2022, Volume and Issue: 288, P. 119351 - 119351

Published: March 14, 2022

Language: Английский

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Treatment of peanut allergy and colitis in mice via the intestinal release of butyrate from polymeric micelles

Nature Biomedical Engineering, Journal Year: 2022, Volume and Issue: 7(1), P. 38 - 55

Published: Dec. 22, 2022

The microbiome modulates host immunity and aids the maintenance of tolerance in gut, where microbial food-derived antigens are abundant. Yet modern dietary factors excessive use antibiotics have contributed to rising incidence food allergies, inflammatory bowel disease other non-communicable chronic diseases associated with depletion beneficial taxa, including butyrate-producing Clostridia. Here we show that intragastrically delivered neutral negatively charged polymeric micelles releasing butyrate different regions intestinal tract restore barrier-protective responses mouse models colitis peanut allergy. Treatment butyrate-releasing increased abundance taxa Clostridium cluster XIVa, protected mice from an anaphylactic reaction a challenge reduced severity T-cell-transfer model colitis. By restoring mucosal homoeostasis, may function as antigen-agnostic approach for treatment allergic diseases.

Language: Английский

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Neurodegenerative and Neurodevelopmental Diseases and the Gut-Brain Axis: The Potential of Therapeutic Targeting of the Microbiome

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9577 - 9577

Published: May 31, 2023

The human gut microbiome contains the largest number of bacteria in body and has potential to greatly influence metabolism, not only locally but also systemically. There is an established link between a healthy, balanced, diverse overall health. When becomes unbalanced (dysbiosis) through dietary changes, medication use, lifestyle choices, environmental factors, ageing, this profound effect on our health linked many diseases, including metabolic inflammatory neurological diseases. While humans largely association dysbiosis with disease, animal models, causative can be demonstrated. brain particularly important maintaining health, strong neurodegenerative neurodevelopmental This suggests that microbiota composition used make early diagnosis diseases modifying microbiome-gut-brain axis might present therapeutic target for have proved intractable, aim altering trajectory such as Alzheimer's Parkinson's multiple sclerosis, autism spectrum disorder, attention-deficit hyperactivity among others. other potentially reversible migraine, post-operative cognitive dysfunction, long COVID, which considered models therapy disease. role traditional methods microbiome, well newer, more novel treatments faecal transplants photobiomodulation, are discussed.

Language: Английский

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The Gut–Organ-Axis Concept: Advances the Application of Gut-on-Chip Technology

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 4089 - 4089

Published: Feb. 17, 2023

The intestine is considered to be a vital digestive organ absorb nutrients and the largest immune organ, while numerous microorganisms coexist with host. It well known that complex interactions between gut microbiota host’s system inevitably affect function of other organs, creating an “axis” them. During past few years, new technique based mainly on microfluidics cell biology has been developed emulate structure, function, microenvironment human gut, called “gut-on-chip”. This microfluidic chip provides insight into key aspects in health disease, such as gut–brain axis, gut–liver gut–kidney gut–lung axis. In this review, we first describe basic theory axis various composition parameter monitoring microarray systems, summarize development emerging advances gut–organ-on-chip, focus host-gut flora nutrient metabolism, highlight their role pathophysiological studies. addition, paper discusses challenges prospects for current further use gut–organ-on-chip platform.

Language: Английский

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Efficient Therapy of Inflammatory Bowel Disease (IBD) with Highly Specific and Durable Targeted Ta₂C Modified with Chondroitin Sulfate (TACS)

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(36)

Published: May 24, 2023

Non-invasive localization of lesions and specific targeted therapy are still the main challenges for inflammatory bowel disease (IBD). Ta, as a medical metal element, has been widely used in treatment different diseases because its excellent physicochemical properties but is far from being explored IBD. Here, Ta2 C modified with chondroitin sulfate (CS) (TACS) evaluated highly nanomedicine Specifically, TACS dual targeting CS functions due to IBD lesion-specific positive charges high expression CD44 receptors. Thanks acid stability, sensitive CT imaging function, strong reactive oxygen species (ROS) elimination ability, oral can accurately locate delineate through non-invasive imaging, specifically treat effectively levels ROS central factor progression As expected, much better therapeutic effects than clinical contrast agent first-line drug 5-aminosalicylic acid, respectively. The mechanism mainly involves protection mitochondria, oxidative stress, inhibiting macrophage M1 polarization, intestinal barrier, restoration flora balance. Collectively, this work provides unprecedented opportunities nanomedicines

Language: Английский

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Role of Akkermansia in Human Diseases: From Causation to Therapeutic Properties

Nutrients, Journal Year: 2023, Volume and Issue: 15(8), P. 1815 - 1815

Published: April 8, 2023

The gut microbiota plays a critical role in the modulation of host metabolism and immune response, its impairment has been implicated many gastrointestinal extraintestinal diseases. Current evidence shows well-documented A. muciniphila maintaining integrity intestinal barrier, modulating improving several metabolic pathways, making it key element pathogenesis human In this scenario, is most promising next-generation probiotic one first microbial species suitable for specific clinical use when compared with traditional probiotics. Further studies are needed to provide more accurate insight into mechanisms action better elucidate properties major areas, paving way integrated personalized therapeutic approach that finally makes our knowledge microbiota.

Language: Английский

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peu-MIR2916-p3-enriched garlic exosomes ameliorate murine colitis by reshaping gut microbiota, especially by boosting the anti-colitic Bacteroides thetaiotaomicron

Pharmacological Research, Journal Year: 2024, Volume and Issue: 200, P. 107071 - 107071

Published: Jan. 11, 2024

Plant-derived exosome-like nanoparticles (ELNs) have drawn considerable attention for oral treatment of colonic diseases. However, the roles ELNs derived from garlic on colitis remain unclear. Here, we demonstrate that (GELNs), with desirable particle sizes (79.60 nm) and trafficking large amounts functional proteins microRNAs, stably roam in gut confer protection against ulcerative (UC). In mice DSS-induced colitis, orally administered GELNs effectively ameliorated bloody diarrhea, normalized production proinflammatory cytokines, prevented barrier impairment. Mechanistically, were taken up by microbes reshaped microbiota dysbiosis, which Bacteroides was dominant respondent genus upon treatment. Notably, GELNs-enriched peu-MIR2916-p3 specifically promoted growth thetaiotaomicron, an intestinal symbiotic bacterium palliative effects colitis. Our findings provide new insights into medicinal application highlight their potential as natural nanotherapeutic agents preventing treating UC.

Language: Английский

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Targeted delivery of the probiotic Saccharomyces boulardii to the extracellular matrix enhances gut residence time and recovery in murine colitis

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 6, 2024

Abstract Probiotic and engineered microbe-based therapeutics are an emerging class of pharmaceutical agents. They represent a promising strategy for treating various chronic inflammatory conditions by interacting with the host immune system and/or delivering therapeutic molecules. Here, we targeted probiotic yeast platform wherein Saccharomyces boulardii is designed to bind abundant extracellular matrix proteins found within lesions gastrointestinal tract through tunable antibody surface display. This approach enabled additional 24–48 h gut residence time compared controls 100-fold increased concentrations colon in preclinical models ulcerative colitis female mice. As result, pharmacodynamic parameters including length, colonic cytokine expression profiles, histological inflammation scores were robustly improved restored back healthy levels. Overall, these studies highlight potential microbial as oral dosage form treatment bowel diseases.

Language: Английский

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Inflammation and bacteriophages affect DNA inversion states and functionality of the gut microbiota

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(3), P. 322 - 334.e9

Published: Feb. 28, 2024

Reversible genomic DNA inversions control the expression of numerous gut bacterial molecules, but how this impacts disease remains uncertain. By analyzing metagenomic samples from inflammatory bowel (IBD) cohorts, we identified multiple invertible regions where a particular orientation correlated with disease. These include promoter polysaccharide A (PSA) Bacteroides fragilis, which induces regulatory T cells (Tregs) and ameliorates experimental colitis. The PSA was mostly oriented "OFF" in IBD patients, increased B. fragilis-associated bacteriophages. Similarly, mice colonized healthy human microbiota induction colitis caused decline "ON" that reversed as inflammation resolved. Monocolonization fragilis revealed bacteriophage infection frequency orientation, causing reduced decreased Treg cells. Altogether, reveal dynamic phase variations driven by bacteriophages host inflammation, signifying functional plasticity during

Language: Английский

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