Nature Communications,
Journal Year:
2022,
Volume and Issue:
13(1)
Published: July 25, 2022
Despite
the
promise
of
immune
checkpoint
inhibition
(ICI),
therapeutic
responses
remain
limited.
This
raises
possibility
that
standard
care
treatments
delivered
in
concert
may
compromise
tumor
response.
To
address
this,
we
employ
tobacco-signature
head
and
neck
squamous
cell
carcinoma
murine
models
which
map
tumor-draining
lymphatics
develop
for
regional
lymphablation
with
surgery
or
radiation.
We
find
eliminates
ICI
response,
worsening
overall
survival
repolarizing
tumor-
peripheral-immune
compartments.
Mechanistically,
within
lymphatics,
observe
an
upregulation
conventional
type
I
dendritic
cells
interferon
signaling
show
both
are
necessary
response
lost
lymphablation.
Ultimately,
provide
a
mechanistic
understanding
how
oncologic
therapies
targeting
impact
to
immune-oncology
therapy
order
define
rational,
lymphatic-preserving
treatment
sequences
mobilize
systemic
antitumor
immunity,
achieve
optimal
responses,
control
metastatic
disease,
confer
durable
immunity.
Annual Review of Immunology,
Journal Year:
2019,
Volume and Issue:
37(1), P. 521 - 546
Published: Feb. 6, 2019
Resident
memory
T
(Trm)
cells
stably
occupy
tissues
and
cannot
be
sampled
in
superficial
venous
blood.
Trm
are
heterogeneous
but
collectively
constitute
the
most
abundant
cell
subset.
form
an
integral
part
of
immune
sensing
network,
monitor
for
local
perturbations
homeostasis
throughout
body,
participate
protection
from
infection
cancer,
likely
promote
autoimmunity,
allergy,
inflammatory
diseases
impede
successful
transplantation.
Thus
major
candidates
therapeutic
manipulation.
Here
we
review
CD8
Cancer Communications,
Journal Year:
2020,
Volume and Issue:
40(4), P. 135 - 153
Published: April 1, 2020
Abstract
Conventional
immunohistochemistry
(IHC)
is
a
widely
used
diagnostic
technique
in
tissue
pathology.
However,
this
associated
with
number
of
limitations,
including
high
inter‐observer
variability
and
the
capacity
to
label
only
one
marker
per
section.
This
review
details
various
highly
multiplexed
techniques
that
have
emerged
circumvent
these
constraints,
allowing
simultaneous
detection
multiple
markers
on
single
section
comprehensive
study
cell
composition,
cellular
functional
cell‐cell
interactions.
Among
techniques,
multiplex
Immunohistochemistry/Immunofluorescence
(mIHC/IF)
has
be
particularly
promising.
mIHC/IF
provides
high‐throughput
staining
standardized
quantitative
analysis
for
reproducible,
efficient
cost‐effective
studies.
immediate
potential
translational
research
clinical
practice,
era
cancer
immunotherapy.
