Controlling Recombination to Evolve Bacteriophages DOI Creative Commons
James J. Bull, Holly A. Wichman,

Stephen M. Krone

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(7), P. 585 - 585

Published: March 28, 2024

Recombination among different phages sometimes facilitates their ability to grow on new hosts. Protocols direct the evolution of phage host range, as might be used in application therapy, would then benefit from including steps enable recombination. Applying mathematical and computational models, addition experiments using T3 T7, we consider ways that a protocol may influence recombination levels. We first address coinfection, which is step enabling The multiplicity infection (MOI, ratio cell concentration) insufficient for predicting (co)infection force (the rate at cells are infected) also critical but more challenging measure. Using both high MOI (>1) ensures levels coinfection. apply four-genetic-locus model study effects recombinant Recombinants accumulate over multiple generations growth, less so if one outgrows other. Supplementing pool with low-fitness recovers some this 'lost' Overall, fine tuning rates will not practical wild phages, qualitative enhancement can attained basic procedures.

Language: Английский

Developing Phage Therapy That Overcomes the Evolution of Bacterial Resistance DOI Creative Commons

Agnès Oromí-Bosch,

Jyot D. Antani, Paul E. Turner

et al.

Annual Review of Virology, Journal Year: 2023, Volume and Issue: 10(1), P. 503 - 524

Published: June 2, 2023

The global rise of antibiotic resistance in bacterial pathogens and the waning efficacy antibiotics urge consideration alternative antimicrobial strategies. Phage therapy is a classic approach where bacteriophages (bacteria-specific viruses) are used against infections, with many recent successes personalized medicine treatment intractable infections. However, perpetual challenge for developing generalized phage expectation that viruses will exert selection target bacteria to deploy defenses virus attack, causing evolution during patient treatment. Here we review two main complementary strategies mitigating therapy: minimizing ability populations evolve driving (steering) phage-resistant toward clinically favorable outcomes. We discuss future research directions might further address phage-resistance problem, foster widespread development deployment therapeutic outsmart evolved clinical settings.

Language: Английский

Citations

62
Antimicrobial resistance: a concise update DOI Creative Commons
Charlotte Shan Ho,

Carlos T H Wong,

Thet Tun Aung

et al.

The Lancet Microbe, Journal Year: 2024, Volume and Issue: unknown, P. 100947 - 100947

Published: Sept. 1, 2024

Language: Английский

Citations

40
The Medicinal Phage—Regulatory Roadmap for Phage Therapy under EU Pharmaceutical Legislation DOI Creative Commons
Timo Faltus

Viruses, Journal Year: 2024, Volume and Issue: 16(3), P. 443 - 443

Published: March 12, 2024

Bacteriophage therapy is a promising approach to treating bacterial infections. Research and development of bacteriophage intensifying due the increase in antibiotic resistance faltering new antibiotics. uses bacteriophages (phages), i.e., prokaryotic viruses, specifically target kill pathogenic bacteria. The legal handling this type raises several questions. These include whether phage therapeutics belong specially regulated class medicinal products, which framework should be followed with regard various technical ways can manufactured administered. article shows products from wild phages genetically modified (designer) do or not belong. Furthermore, explains relevant for manufacture administration therapeutics, are advance uniform, patient-independent manner, tailor-made patient-specific therapeutics. For systematically coherent, successful translation therapy, considers pharmaceutical law related areas, such as genetic engineering law. Finally, how planned legislative revisions Directive 2001/83/EC Regulation (EC) No 726/2004 may affect future therapy.

Language: Английский

Citations

19
A Comprehensive Review on Phage Therapy and Phage-Based Drug Development DOI Creative Commons
Longzhu Cui, Shinya Watanabe, Kazuhiko Miyanaga

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(9), P. 870 - 870

Published: Sept. 11, 2024

Phage therapy, the use of bacteriophages (phages) to treat bacterial infections, is regaining momentum as a promising weapon against rising threat multidrug-resistant (MDR) bacteria. This comprehensive review explores historical context, modern resurgence phage and phage-facilitated advancements in medical technological fields. It details mechanisms action applications phages treating MDR particularly those associated with biofilms intracellular pathogens. The further highlights innovative uses vaccine development, cancer gene delivery vectors. Despite its targeted efficient approach, therapy faces challenges related stability, immune response, regulatory approval. By examining these areas detail, this underscores immense potential remaining hurdles integrating phage-based therapies into practices.

Language: Английский

Citations

17
Bacteriophage therapy in aquaculture: current status and future challenges DOI
Ruyin Liu,

Ganghua Han,

Zong Li

et al.

Folia Microbiologica, Journal Year: 2022, Volume and Issue: 67(4), P. 573 - 590

Published: March 19, 2022

Language: Английский

Citations

50
Mitigation of evolved bacterial resistance to phage therapy DOI Creative Commons
Clara Torres-Barceló, Paul E. Turner, Angus Buckling

et al.

Current Opinion in Virology, Journal Year: 2022, Volume and Issue: 53, P. 101201 - 101201

Published: Feb. 15, 2022

Language: Английский

Citations

45
Phage Products for Fighting Antimicrobial Resistance DOI Creative Commons

Yuanling Huang,

Wenhui Wang,

Zhihao Zhang

et al.

Microorganisms, Journal Year: 2022, Volume and Issue: 10(7), P. 1324 - 1324

Published: June 30, 2022

Antimicrobial resistance (AMR) has become a global public health issue and antibiotic agents have lagged behind the rise in bacterial resistance. We are searching for new method to combat AMR phages viruses that can effectively fight infections, which renewed interest as alternatives with their specificity. Large phage products been produced recent years AMR. Using “one health” approach, this review summarizes used plant, food, animal, human health. In addition, advantages disadvantages future perspectives development of therapy an alternative also discussed review.

Language: Английский

Citations

45
The Future of Clinical Phage Therapy in the United Kingdom DOI Creative Commons
Joshua D. Jones,

Clare Trippett,

Mehrunisha Suleman

et al.

Viruses, Journal Year: 2023, Volume and Issue: 15(3), P. 721 - 721

Published: March 10, 2023

Bacteriophage (phage) therapy is a promising alternative antimicrobial strategy with the potential to transform way bacterial infections are treated. In United Kingdom, phages classed as biological medicine. Although no licensed for UK use, they may be used unlicensed medicinal products where alternatives cannot meet patient’s clinical needs. last 2 years, 12 patients in have received phage therapy, and there burgeoning interest. Currently, provision ad hoc relies upon networking international sources of phages. The will not progress beyond an increasing number cases until onshore sustainable scalable source well-characterised manufactured accordance Good Manufacturing Practice (GMP) established. Here, we present exciting new collaboration between Phage Therapy, Centre Research at University Leicester, CPI, Fixed Phage. These partners, others develop, establish sustainable, scalable, equitable UK. We set out vision how integrated into NHS healthcare more broadly, including complementarity (cocktail) (personalised) preparations. Key elements infrastructure GMP manufacturing, national library, centre. Together, this support microbiology departments develop oversee across As it take time deliver this, also describe considerations clinicians seeking use interim. summary, review sets roadmap delivery UK, benefits which hope reverberate decades come.

Language: Английский

Citations

35
Rapid bacteria-phage coevolution drives the emergence of multiscale networks DOI
Joshua M. Borin, Justin Lee, Adriana Lucía-Sanz

et al.

Science, Journal Year: 2023, Volume and Issue: 382(6671), P. 674 - 678

Published: Nov. 9, 2023

Interactions between species catalyze the evolution of multiscale ecological networks, including both nested and modular elements that regulate function diverse communities. One common assumption is such complex pattern formation requires spatial isolation or long evolutionary timescales. We show network structure can evolve rapidly under simple conditions without structure. In just 21 days laboratory coevolution, Escherichia coli bacteriophage Φ21 coevolve diversify to form elaborate cross-infection networks. By measuring ~10,000 phage-bacteria infections testing genetic basis interactions, we identify mechanisms create each component pattern. Our results demonstrate how networks in parasite-host systems, illustrating Darwin's idea adaptive processes generate entangled banks interactions.

Language: Английский

Citations

25
Open-endedness in synthetic biology: A route to continual innovation for biological design DOI Creative Commons
Michiel Stock, Thomas E. Gorochowski

Science Advances, Journal Year: 2024, Volume and Issue: 10(3)

Published: Jan. 19, 2024

Design in synthetic biology is typically goal oriented, aiming to repurpose or optimize existing biological functions, augmenting with new-to-nature capabilities, creating life-like systems from scratch. While the field has seen many advances, bottlenecks complexity of built are emerging and designs that function lab often fail when used real-world contexts. Here, we propose an open-ended approach design, novelty designed being at least as important how well it fulfils its goal. Rather than solely focusing on optimization toward a single best designing mind may allow us move beyond diminishing returns see performance for most engineered biology. Research artificial life community demonstrated embracing can automatically generate innovative unexpected solutions challenging problems local optima. Synthetic offers ideal playground explore more creative approaches design.

Language: Английский

Citations

10