A detailed in silico analysis of secondary metabolite biosynthesis clusters in the genome of the broad host range plant pathogenic fungus Sclerotinia sclerotiorum DOI Creative Commons
Carolyn Graham-Taylor, Lars G. Kamphuis, Mark C. Derbyshire

et al.

BMC Genomics, Journal Year: 2020, Volume and Issue: 21(1)

Published: Jan. 2, 2020

The broad host range pathogen Sclerotinia sclerotiorum infects over 400 plant species and causes substantial yield losses in crops worldwide. Secondary metabolites are known to play important roles the virulence of pathogens, but little is about secondary metabolite repertoire S. sclerotiorum. In this study, we predicted biosynthetic gene clusters genome analysed their expression during infection Brassica napus using an existing transcriptome data set. We also investigated sequence diversity among a panel 25 previously published isolate genomes.We identified 80 putative clusters. Over half contained at least three transcriptionally coregulated genes. Comparative genomics revealed homologous closely related Botrytis cinerea for production carotenoids, hydroxamate siderophores, DHN melanin botcinic acid. phytotoxin that can potentially produce polyketide sclerin epipolythiodioxopiperazine. were enriched subtelomeric genomic regions, those containing paralogues showed particularly strong association with repeats. positional bias was borne out by intraspecific comparisons genes suffered more presence / absence polymorphisms exhibited significantly higher than other genes.These suggest produces numerous undergo enhanced rates mutation, duplication recombination regions. microevolutionary regimes leading have yet be elucidated. Several potential phytotoxins documented study provide basis future functional analyses.

Language: Английский

Vayg1 is required for microsclerotium formation and melanin production in Verticillium dahliae DOI
Rong Fan, Steven J. Klosterman,

Conghao Wang

et al.

Fungal Genetics and Biology, Journal Year: 2016, Volume and Issue: 98, P. 1 - 11

Published: Nov. 17, 2016

Language: Английский

Citations

66
The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6 DOI
Antoine Porquier,

G. Morgant,

Javier Moraga

et al.

Fungal Genetics and Biology, Journal Year: 2016, Volume and Issue: 96, P. 33 - 46

Published: Oct. 10, 2016

Language: Английский

Citations

65
Transcription factor VdCmr1 is required for pigment production, protection from UV irradiation, and regulates expression of melanin biosynthetic genes in Verticillium dahliae DOI Creative Commons
Yonglin Wang, Xiao Hu,

Yulin Fang

et al.

Microbiology, Journal Year: 2018, Volume and Issue: 164(4), P. 685 - 696

Published: Feb. 27, 2018

Verticillium dahliae is a soilborne fungus that causes vascular wilt diseases on numerous plant species worldwide. The production of darkly melanized microsclerotia crucial in the disease cycle V. dahliae, as these structures allow for long-term survival soil. Previously, transcriptomic and genomic analysis identified cluster genes encodes some dihydroxynaphthalene (DHN) melanin biosynthetic pathway homologues found related fungi. In this study, we explored roles cluster-specific transcription factor VdCmr1, well two other within encoding polyketide synthase (VdPKS1) laccase (VdLac1), enzymes at initial endpoint steps DHN production. results revealed VdCmr1 VdPKS1 are required production, but neither None three were pathogenesis tobacco lettuce. Exposure ΔVdCmr1 wild-type strains to UV irradiation, or high temperature (40 °C), an approx. 50 % reduction strain, relative response either condition. Expression profiles expression part dependent VdCmr1. Combined data indicate key regulator biosynthesis, via regulation melanogenesis, affects abiotic threats. We conclude with model showing by osmolarity glycerol (Hog)-type MAP kinase pathway.

Language: Английский

Citations

64
Surface Dielectric Barrier Discharge plasma: a suitable measure against fungal plant pathogens DOI Creative Commons
Paolo F. Ambrico, Milan Šimek, Caterina Rotolo

et al.

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: Feb. 28, 2020

Abstract Fungal diseases seriously affect agricultural production and the food industry. Crop protection is usually achieved by synthetic fungicides, therefore more sustainable innovative technologies are increasingly required. The atmospheric pressure low-temperature plasma a novel suitable measure. We report on effect of treatment phytopathogenic fungi causing quantitative qualitative losses products both in field postharvest. focus our attention vitro direct inhibitory non-contact Surface Dielectric Barrier Discharge conidia germination Botrytis cinerea , Monilinia fructicola Aspergillus carbonarius Alternaria alternata . A few minutes was required to completely inactivate an artificial medium. Morphological analysis spores Scanning Electron Microscopy suggests that main mechanism etching due Reactive Oxygen Species or UV radiation. Spectroscopic generated humid air gives hint rotational temperature gas should not play relevant role being very close room temperature. In vivo experiments artificially inoculated cherry fruits demonstrated inactivation fungal extend their shelf life. Pre-treatment before inoculation improve resistance infections maybe activating defense responses plant tissues.

Language: Английский

Citations

63
Colors of life: a review on fungal pigments DOI
Haritha Meruvu, Joana Carolina Freire Sandes Santos

Critical Reviews in Biotechnology, Journal Year: 2021, Volume and Issue: 41(8), P. 1153 - 1177

Published: April 8, 2021

Colorants find social and commercial applications in cosmetics, food, pharmaceuticals, textiles, other industrial sectors. Among the available options, chemically synthesized colorants are popular due to their low-cost flexible production modes, but health environmental concerns have encouraged valorization of biopigments that natural ecofriendly. biopigment producers, microorganisms noteworthy for all-seasonal stable pigments with high-yield titers. Fungi paramount sources pigments. They occupy diverse ecological niches adaptive metabolisms biocatalytic pathways, making them entities an interest. Industrially important like carotenoids, melanins, riboflavins, azaphilones, quinones produced by filamentous fungi described within context this review. Most recent information about fungal pigment characteristics, biochemical routes potential applications, limitations, future research perspectives described.

Language: Английский

Citations

55
Fungal Melanin Biosynthesis Pathway as Source for Fungal Toxins DOI Creative Commons
Jia Gao,

Max Wenderoth,

Maria Doppler

et al.

mBio, Journal Year: 2022, Volume and Issue: 13(3)

Published: April 27, 2022

Contamination of food and feed with toxin-producing fungi is a major threat in agriculture for human health. The filamentous fungus Alternaria alternata one the most widespread postharvest contaminants weak plant pathogen. It produces large variety secondary metabolites alternariol its derivatives as characteristic mycotoxin. Other important phyto- mycotoxins are perylene quinones (PQs), some which have anticancer properties. Here, we discovered that PQ altertoxin (ATX) biosynthesis shares enzymes 1,8-dihydroxynaphthalene (1,8-DHN) melanin pathway. However, was formed aerial hyphae spores, ATXs were synthesized substrate hyphae. This spatial separation achieved through promiscuity polyketide synthase, presumably producing pentaketide (T4HN), hexaketide (AT4HN), heptaketide (YWA1) products. T4HN directly enters DHN pathway, whereas AT4HN YWA1 can be converted only hyphae, probably leads to higher concentration, favoring 1,8-DHN formation. Whereas production strictly dependent on CmrA transcription factor, could still produced absence extent. suggests different cues regulate toxin Since by many fungi, PQs or related compounds may more than so far assumed. IMPORTANCE Mycotoxins Food safety control relies identification toxins detection expression respective genes. latter method, however, knowledge biosynthetic pathway key contaminant altertoxins other prominent examples. dihydroxynaphthalene (DHN) Because among quinone class anticipated.

Language: Английский

Citations

29
From Genes to Molecules, Secondary Metabolism in Botrytis cinerea: New Insights into Anamorphic and Teleomorphic Stages DOI Creative Commons
Haroldo da Silva Ripardo-Filho, Víctor Coca-Ruíz, Ivonne Suárez

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(3), P. 553 - 553

Published: Jan. 26, 2023

The ascomycete Botrytis cinerea Pers. Fr., classified within the family Sclerotiniaceae, is agent that causes grey mould disease which infects at least 1400 plant species, including crops of economic importance such as grapes and strawberries. life cycle B. consists two phases: asexual (anamorph, Fr.) sexual (teleomorph, Botryotinia fuckeliana (de Bary) Wetzel). During XVI International Symposium dedicated to fungus, was held in Bari June 2013, scientific community unanimously decided assign most widely used name form, Botrytis, this genus fungi. However, literature, we continue find articles referring both morphic stages. In review, take stock genes metabolites reported for forms between January 2015 October 2022.

Language: Английский

Citations

18
Insights into the Biocontrol Function of a Burkholderia gladioli Strain against Botrytis cinerea DOI Creative Commons
Dan Wang, Wanzhen Luo, Dandan Zhang

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: March 2, 2023

Searching the economical, eco-friendly and efficient biological control measures is key to protecting crops from pathogenic fungi. The species of Burkholderia genus are widespread in natural environment, which nonpathogenic members have been reported great potential for agents biofertilizers agricultural application. gladioli strains, however, need more study application fungi, plant growth promotion, induced systemic resistance (ISR).

Language: Английский

Citations

17
Antimicrobial peptide-based strategies to overcome antimicrobial resistance DOI

Meetali Girdhar,

Aparajita Sen, Arti Nigam

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(10)

Published: Sept. 23, 2024

Language: Английский

Citations

6
Functional analysis of polyketide synthase genes in the biocontrol fungus Clonostachys rosea DOI Creative Commons

Umma Fatema,

Anders Broberg, Dan Funck Jensen

et al.

Scientific Reports, Journal Year: 2018, Volume and Issue: 8(1)

Published: Oct. 3, 2018

Clonostachys rosea is a mycoparasitic fungus used for biological control of plant diseases. Its genome contains 31 genes putatively encoding polyketide synthases (PKSs), 75% which are arranged in biosynthetic gene clusters. Gene expression analysis during C. interactions with the fungal pathogens Botrytis cinerea and Fusarium graminearum showed common species-specific induction PKS genes. Our data culture media dependent correlation between degree antagonism rosea. The pks22 pks29 were highly induced fungal-fungal but not pigmentation, deletion studies revealed that PKS29 was required full against B. cinerea, biocontrol fusarium foot rot on barley. Metabolite Δpks29 strains has 50% reduced production (P = 0.001) an unknown molecular formula C15H28O3, while Δpks22 lost ability to produce four previously polyketides named Clonorosein A-D. A B purified, their structures determined, strong antifungal activity F. graminearum. These results show PKS22 A-D, demonstrate role

Language: Английский

Citations

56