Multiple Lines of Evidence Indicate That Gliotransmission Does Not Occur under Physiological Conditions DOI Creative Commons
Todd A. Fiacco,

Ken D. McCarthy

Journal of Neuroscience, Journal Year: 2018, Volume and Issue: 38(1), P. 3 - 13

Published: Jan. 3, 2018

A major controversy persists within the field of glial biology concerning whether or not, under physiological conditions, neuronal activity leads to Ca2+-dependent release neurotransmitters from astrocytes, a phenomenon known as gliotransmission. Our perspective is that, while we and others can apply techniques cause gliotransmission, there considerable evidence gathered using astrocyte-specific more approaches which suggests that gliotransmission pharmacological rather than process. Approaches providing against include stimulation Gq-GPCRs expressed only in well removal primary proposed source astrocyte Ca2+ responsible for These contrast with those supportive mechanical stimulation, strong astrocytic depolarization whole-cell patch-clamp optogenetics, uncaging IP3, chelating BAPTA, nonspecific bath application agonists receptors by multitude cell types. are not subtle therefore recent suggestions requires very specific delicate temporal spatial requirements. Other evidence, including lack propagating waves between astrocytes healthy tissue, expression vesicular machinery, demise d-serine hypothesis, provides additional Overall, data suggest province neurons, intact brain conditions.Dual Perspectives Companion Paper: Gliotransmission: Beyond Black-and-White, Iaroslav Savtchouk Andrea Volterra.

Language: Английский

Neural Circuit-Specialized Astrocytes: Transcriptomic, Proteomic, Morphological, and Functional Evidence DOI Creative Commons

Hua Chai,

Blanca Díaz‐Castro, Eiji Shigetomi

et al.

Neuron, Journal Year: 2017, Volume and Issue: 95(3), P. 531 - 549.e9

Published: July 14, 2017

Astrocytes are ubiquitous in the brain and widely held to be largely identical. However, this view has not been fully tested, possibility that astrocytes neural circuit specialized remains unexplored. Here, we used multiple integrated approaches, including RNA sequencing (RNA-seq), mass spectrometry, electrophysiology, immunohistochemistry, serial block-face-scanning electron microscopy, morphological reconstructions, pharmacogenetics, diffusible dye, calcium, glutamate imaging, directly compare adult striatal hippocampal under identical conditions. We found significant differences electrophysiological properties, Ca2+ signaling, morphology, astrocyte-synapse proximity between astrocytes. Unbiased evaluation of actively translated proteomic data confirmed astrocyte diversity circuits. thus report core reveal evidence for within circuits, provide new, database resources approaches explore function throughout brain.Video AbstracteyJraWQiOiI4ZjUxYWNhY2IzYjhiNjNlNzFlYmIzYWFmYTU5NmZmYyIsImFsZyI6IlJTMjU2In0.eyJzdWIiOiI0OGY5MjVkMzgwOGU0NTkwZGMzNTRhMjQwZjZkMWM0OCIsImtpZCI6IjhmNTFhY2FjYjNiOGI2M2U3MWViYjNhYWZhNTk2ZmZjIiwiZXhwIjoxNjc3ODAzMzg0fQ.MwSBbMAp8Z94bVovEvEtEK9rSaCme1FWQaVStp6O8krax6PBRClksqtYhGWrI6xK8ct9tTpHJOavNya9agX01pfBV5Xv2_BxcAlr-eYdkIlnW6ddiNKQ9_7eEHZulgpmyVbQq2DLjKc1qNoV1ABBLI5qYU4yHQPnu-EI2_RASm5h1NErzRmbGIHeglc9V82L8hZBX8NyvUw74JxMPzvU4cgxuX5JBvnXBUOvw2oTkF0oPeBvpSchW6EKQzen4Skxhu-LXb5aAa3LkCMU3JhcCep28BCCVsKkV1kx3RuXhdJgkWD2ucI-70LBWqUvhtG-49ThFs0TB8dIBr74DrFLKA(mp4, (55.46 MB) Download video

Language: Английский

Citations

685
Astrocyte function from information processing to cognition and cognitive impairment DOI
Mirko Santello, Nicolas Toni, Andrea Volterra

et al.

Nature Neuroscience, Journal Year: 2019, Volume and Issue: 22(2), P. 154 - 166

Published: Jan. 21, 2019

Language: Английский

Citations

596
Functional diversity of astrocytes in neural circuit regulation DOI
Lucile Ben Haim, David H. Rowitch

Nature reviews. Neuroscience, Journal Year: 2016, Volume and Issue: 18(1), P. 31 - 41

Published: Dec. 1, 2016

Language: Английский

Citations

534
Function and therapeutic value of astrocytes in neurological diseases DOI
Hong‐Gyun Lee, Michael A. Wheeler, Francisco J. Quintana

et al.

Nature Reviews Drug Discovery, Journal Year: 2022, Volume and Issue: 21(5), P. 339 - 358

Published: Feb. 16, 2022

Language: Английский

Citations

371
Astrocytes, neurons, synapses: a tripartite view on cortical circuit development DOI Creative Commons
Isabella Farhy-Tselnicker, Nicola J. Allen

Neural Development, Journal Year: 2018, Volume and Issue: 13(1)

Published: April 30, 2018

In the mammalian cerebral cortex neurons are arranged in specific layers and form connections both within with other brain regions, thus forming a complex mesh of specialized synaptic comprising distinct circuits. The correct establishment these during development is crucial for proper function brain. Astrocytes, major type glial cell, important regulators synapse formation development. While neurogenesis precedes astrogenesis cortex, neuronal synapses only begin to after astrocytes have been generated, concurrent branching process elaboration. Here we provide combined overview developmental processes circuit rodent emphasizing timeline astrocytic maturation. We further discuss role at synapse, focusing on astrocyte-synapse contact synapse-related proteins promoting cortical

Language: Английский

Citations

340
Reducing Astrocyte Calcium Signaling In Vivo Alters Striatal Microcircuits and Causes Repetitive Behavior DOI Creative Commons
Xinzhu Yu, Anna M.W. Taylor, Jun Nagai

et al.

Neuron, Journal Year: 2018, Volume and Issue: 99(6), P. 1170 - 1187.e9

Published: Aug. 30, 2018

Language: Английский

Citations

321
Gliotransmission: Beyond Black-and-White DOI Creative Commons
Iaroslav Savtchouk, Andrea Volterra

Journal of Neuroscience, Journal Year: 2018, Volume and Issue: 38(1), P. 14 - 25

Published: Jan. 3, 2018

Astrocytes are highly complex cells with many emerging putative roles in brain function. Of these, gliotransmission (active information transfer from glia to neurons) has probably the widest implications on our understanding of how works: do astrocytes really contribute processing within neural circuitry? “Positive evidence” for this stems work multiple laboratories reporting examples modulatory chemical signaling neurons timeframe hundreds milliseconds several minutes. This involves, but is not limited to, Ca 2+ -dependent vesicular transmitter release, and results a variety regulatory effects at synapses circuits that abolished by preventing elevations or blocking exocytosis selectively astrocytes. In striking contradiction, methodologically advanced studies few produced “negative evidence,” triggering heated debate actual existence properties gliotransmission. context, skeptics' camp arose, eager dismiss whole positive evidence based number assumptions behind negative data, such as following: (1) deleting single release pathway (IP3R2) removes all sources gliotransmission; (2) stimulating transgenically expressed Gq-GPCR (MrgA1) mimics physiological underlying gliotransmitter release; (3) age-dependent downregulation an endogenous GPCR (mGluR5) questions adulthood; (4) failure transcriptome analysis detect vGluts canonical synaptic SNAREs proves inexistence/functional irrelevance release. We here discuss above likely wrong oversimplistic. light most recent literature, we argue more phenomenon than originally thought, possibly consisting forms processes, whose correct study require sophisticated tools finer scientific experiments done until today. Under perspective, opposing camps can be reconciled field moved forward. Along path, cautious mindset attitude open discussion mutual respect between opponent will good companions. Dual Perspectives Companion Paper: Multiple Lines Evidence Indicate That Gliotransmission Does Not Occur under Physiological Conditions, Todd A. Fiacco Ken D. McCarthy

Language: Английский

Citations

308
Synapse-specific astrocyte gating of amygdala-related behavior DOI
Mario Martín‐Fernández,

Stephanie Jamison,

Laurie M. Robin

et al.

Nature Neuroscience, Journal Year: 2017, Volume and Issue: 20(11), P. 1540 - 1548

Published: Sept. 25, 2017

Language: Английский

Citations

302
Hyperactivity with Disrupted Attention by Activation of an Astrocyte Synaptogenic Cue DOI Creative Commons
Jun Nagai, Abha K. Rajbhandari, Mohitkumar R. Gangwani

et al.

Cell, Journal Year: 2019, Volume and Issue: 177(5), P. 1280 - 1292.e20

Published: April 25, 2019

Language: Английский

Citations

298
Diversity and Specificity of Astrocyte–neuron Communication DOI

Caitlin A. Durkee,

Alfonso Araque

Neuroscience, Journal Year: 2018, Volume and Issue: 396, P. 73 - 78

Published: Nov. 17, 2018

Language: Английский

Citations

282