Advances in Electrochemical Biosensors Based on Nanomaterials for Protein Biomarker Detection in Saliva DOI Creative Commons
Tao Dong, Nuno Pires,

Zhaochu Yang

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 10(6)

Published: Dec. 30, 2022

Abstract The focus on precise medicine enhances the need for timely diagnosis and frequent monitoring of chronic diseases. Moreover, recent pandemic severe acute respiratory syndrome coronavirus 2 poses a great demand rapid detection surveillance viral infections. protein biomarkers antigens in saliva allows identification diseases or disease changes scenarios where when test response at point care is mandated. While traditional methods testing fail to provide desired fast results, electrochemical biosensors based nanomaterials hold perfect characteristics point‐of‐care settings. advances sensors salivary are critically reviewed this work, with emphasis role boost biosensor analytical performance increase reliability human samples. Furthermore, work identifies critical factors further modernization nanomaterial‐based sensors, envisaging development implementation next‐generation sample‐in‐answer‐out systems.

Language: Английский

Aging in COVID-19: Vulnerability, immunity and intervention DOI Creative Commons
Yiyin Chen, Sabra L. Klein, Brian T. Garibaldi

et al.

Ageing Research Reviews, Journal Year: 2020, Volume and Issue: 65, P. 101205 - 101205

Published: Oct. 31, 2020

Language: Английский

Citations

915
Persistence of serum and saliva antibody responses to SARS-CoV-2 spike antigens in COVID-19 patients DOI Creative Commons
Baweleta Isho,

Kento T. Abe,

Michelle Zuo

et al.

Science Immunology, Journal Year: 2020, Volume and Issue: 5(52)

Published: Oct. 8, 2020

While the antibody response to SARS-CoV-2 has been extensively studied in blood, relatively little is known about saliva and its relationship systemic levels. Here, we profiled by enzyme-linked immunosorbent assays (ELISAs) IgG, IgA IgM responses spike protein (full length trimer) receptor-binding domain (RBD) serum of acute convalescent patients with laboratory-diagnosed COVID-19 ranging from 3-115 days post-symptom onset (PSO), compared negative controls. Anti-SARS-CoV-2 were readily detected saliva, peak IgG levels attained 16-30 PSO. Longitudinal analysis revealed that anti-SARS-CoV-2 antibodies rapidly decayed, while remained stable up 105 PSO both biofluids. Lastly, a lesser extent RBD positively correlated matched samples. This study confirms are maintained majority for at least 3 months may serve as surrogate measure immunity based on their correlation responses.

Language: Английский

Citations

835
Diagnostics for SARS-CoV-2 infections DOI Creative Commons
Bhavesh D. Kevadiya, Jatin Machhi, Jonathan Herskovitz

et al.

Nature Materials, Journal Year: 2021, Volume and Issue: 20(5), P. 593 - 605

Published: Feb. 15, 2021

Language: Английский

Citations

727
New-onset IgG autoantibodies in hospitalized patients with COVID-19 DOI Creative Commons
Sarah E. Chang, Allan Feng, Wenzhao Meng

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: Sept. 14, 2021

COVID-19 is associated with a wide range of clinical manifestations, including autoimmune features and autoantibody production. Here we develop three protein arrays to measure IgG autoantibodies connective tissue diseases, anti-cytokine antibodies, anti-viral antibody responses in serum from 147 hospitalized patients. Autoantibodies are identified approximately 50% patients but less than 15% healthy controls. When present, largely target autoantigens rare disorders such as myositis, systemic sclerosis overlap syndromes. A subset targeting traditional or cytokines de novo following SARS-CoV-2 infection. track longitudinal development antibodies recognizing structural proteins non-structural proteins, not influenza, seasonal coronaviruses other pathogenic viruses. We conclude that causes new-onset significant proportion positively correlated immune proteins.

Language: Английский

Citations

400
Tools and Techniques for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/COVID-19 Detection DOI
Seyed Hamid Safiabadi Tali, Jason J. LeBlanc,

Zubi Sadiq

et al.

Clinical Microbiology Reviews, Journal Year: 2021, Volume and Issue: 34(3)

Published: May 11, 2021

The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory 2 (SARS-CoV-2), has led to millions of confirmed cases and deaths worldwide. Efficient diagnostic tools are in high demand, as rapid large-scale testing plays a pivotal role patient management decelerating spread.

Language: Английский

Citations

347
S Protein-Reactive IgG and Memory B Cell Production after Human SARS-CoV-2 Infection Includes Broad Reactivity to the S2 Subunit DOI Creative Commons
Phuong Nguyen‐Contant, A. Karim Embong, Preshetha Kanagaiah

et al.

mBio, Journal Year: 2020, Volume and Issue: 11(5)

Published: Sept. 28, 2020

The recent rapid worldwide spread of SARS-CoV-2 has established a pandemic potentially serious disease in the highly susceptible human population. Key issues are whether humans have preexisting immune memory that provides some protection against and infection generates lasting reinfection. Our analysis focused on pre- postinfection IgG B cells (MBCs) reactive to proteins. Most importantly, we demonstrate both MBCs novel receptor binding domain conserved S2 subunit spike protein. Thus, even if antibody levels wane, long-lived remain mediate production. study results also suggest strengthens broad coronavirus through S2-reactive MBC formation.

Language: Английский

Citations

211
COVID-19 Antibody Tests and Their Limitations DOI Open Access
Guoqiang Liu, James F. Rusling

ACS Sensors, Journal Year: 2021, Volume and Issue: 6(3), P. 593 - 612

Published: Feb. 5, 2021

COVID-19, caused by the SARS-CoV-2 virus, has developed into a global health crisis, causing over 2 million deaths and changing people's daily life world over. Current main-stream diagnostic methods in laboratory include nucleic acid PCR tests direct viral antigen for detecting active infections, indirect human antibody specific to detect prior exposure. In this Perspective, we briefly describe then focus mainly on existing their limitations including inaccuracies possible causes of unreliability. False negatives immunoassays can arise from assay formats, selection antigens types, testing windows, individual variance, fluctuation levels. Reasons false positives involve cross-reactivity other viruses, as well autoimmune disease. The spectrum bias an effect both positives. For developers, not only improvement formats but also isotopes antibodies need be carefully considered improve sensitivity specificity. clinicians, factors influencing accuracy assays must kept mind test patients using currently imperfect available with smart tactics realistic interpretation results.

Language: Английский

Citations

200
Antibody tests for identification of current and past infection with SARS-CoV-2 DOI
Tilly Fox, Julia Geppert, Jacqueline Dinnes

et al.

Cochrane library, Journal Year: 2022, Volume and Issue: 2022(11)

Published: Nov. 17, 2022

Language: Английский

Citations

178
Microfluidic chain reaction of structurally programmed capillary flow events DOI Creative Commons

Mohamed Yafia,

Oriol Ymbern, Ayokunle O. Olanrewaju

et al.

Nature, Journal Year: 2022, Volume and Issue: 605(7910), P. 464 - 469

Published: May 18, 2022

Chain reactions, characterized by initiation, propagation and termination, are stochastic at microscopic scales underlie vital chemical (for example, combustion engines), nuclear biotechnological polymerase chain reaction) applications1–5. At macroscopic scales, reactions deterministic limited to applications for entertainment art such as falling dominoes Rube Goldberg machines. On the other hand, microfluidic lab-on-a-chip (also called a micro-total analysis system)6,7 was visualized an integrated chip, akin microelectronic circuits, yet in practice remains dependent on cumbersome peripherals, connections computer automation8–11. Capillary microfluidics integrate energy supply flow control onto single chip using capillary phenomena, but programmability rudimentary with most handful (eight) operations possible12–19. Here we introduce reaction (MCR) conditional, structurally programmed of events. Monolithic chips integrating MCR three-dimensionally printed, powered free paper pump, autonomously execute liquid handling algorithms step-by-step. With MCR, automated (1) sequential release 300 aliquots across chained, interconnected chips, (2) protocol severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) antibodies detection saliva (3) thrombin generation assay continuous subsampling coagulation-activated plasma parallel including timers, iterative cycles synchronous stop-flow operations. MCRs untethered from unencumbered encode programs situ can form frugal, versatile, bona fide wide-ranging point-of-care diagnostics. Microfluidic situ, diagnostics

Language: Английский

Citations

136
COVID-19 salivary Raman fingerprint: innovative approach for the detection of current and past SARS-CoV-2 infections DOI Creative Commons
Cristiano Carlomagno,

Dario Bertazioli,

Alice Gualerzi

et al.

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: March 2, 2021

The pandemic of COVID-19 is continuously spreading, becoming a worldwide emergency. Early and fast identification subjects with current or past infection must be achieved to slow down the epidemiological widening. Here we report Raman-based approach for analysis saliva, able significantly discriminate signal patients by from healthy and/or infection. Our results demonstrated differences in saliva biochemical composition three experimental groups, modifications grouped specific attributable spectral regions. classification model was collected accuracy, precision, sensitivity specificity more than 95%. In order translate this discrimination signal-level patient-level, developed Deep Learning obtaining accuracy range 89-92%. These findings have implications creation potential diagnostic tool, using as minimal invasive highly informative biofluid, demonstrating efficacy model.

Language: Английский

Citations

131