Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12
Published: June 29, 2022
in the family Botryosphaeriaceae is a cosmopolitan phytopathogenic fungus and responsible for causing cankers, fruit rot leaf spots on economically important plants. In this study, we characterized virome of single Pakistani strain (L3)
Language: Английский
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: June 4, 2024
Abstract Non-self recognition is a fundamental aspect of life, serving as crucial mechanism for mitigating proliferation molecular parasites within fungal populations. However, studies investigating the potential interference plants with non-self mechanisms are limited. Here, we demonstrate pronounced increase in efficiency horizontal mycovirus transmission between vegetatively incompatible Sclerotinia sclerotiorum strains planta compared to vitro. This increased associated elevated proline concentration following S. infection. surge levels attenuates reaction among fungi by inhibition cell death, thereby facilitating transmission. Furthermore, our field experiments reveal that combined deployment hypovirulent harboring hypovirulence-associated mycoviruses (HAVs) together exogenous confers substantial protection oilseed rape against virulent . unprecedented discovery illuminates novel pathway which can counteract infection, leveraging weakening and promotion HAVs spread. These promising insights provide an avenue explore developing innovative biological control strategies aimed at diseases enhancing efficacy HAV
Language: Английский
Citations
21
mBio, Journal Year: 2024, Volume and Issue: 15(2)
Published: Jan. 9, 2024
Colletotrichum is a kind of economically important phytopathogenic fungi that cause anthracnose disease in variety plant species worldwide. We found novel mycovirus the Gammapartitiviru s genus and Partitiviridae family from fungus alienum named it CaPV1. This study revealed CaPV1 infection significantly decreased host virulence fitness by affecting mycelial growth, appressorial development, appressorium turgor. In addition, could also infect other species, including C. fructicola , spaethianum gloeosporioides viral particle transfection resulting hypovirulence these species. Transcriptomic analysis showed caused significant transcriptional rewiring especially genes involved vesicle transport. Moreover, endocytosis gene knockout assays demonstrated mechanism underlying CaPV1-induced is, at least part, transport fungus. provided insights into mechanisms pathogenesis mycovirus-fungus interactions, linking role systems shaping fungal pathogenicity.
Language: Английский
Citations
19
Advances in virus research, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 86
Published: Jan. 1, 2023
Language: Английский
Citations
32
Advanced Science, Journal Year: 2023, Volume and Issue: 10(29)
Published: Aug. 16, 2023
Abstract Mycovirus‐mediated hypovirulence has the potential to control fungal diseases. However, availability of hypovirulence‐conferring mycoviruses for plant disease is limited as most viruses are asymptomatic. In this study, virus‐induced gene silencing (VIGS) vector p26‐D4 Fusarium graminearum gemytripvirus 1 (FgGMTV1), a tripartite circular single‐stranded DNA mycovirus, successfully constructed convert causal fungus cereal head blight (FHB) into hypovirulent strain. p26‐D4, with an insert 75–150 bp fragment target reporter transgene transcript in both sense and antisense orientations, efficiently triggered . Notably, two strains, p26‐D4‐Tri101, p26‐D4‐FgPP1, obtained by virulence‐related genes Tri101 FgPP1 can be used biocontrol agents protect wheat from FHB mycotoxin contamination at field level. This study not only describes first mycovirus‐derived VIGS system but also proves that establish multiple strains pathogenic fungi.
Language: Английский
Citations
25
PLoS Pathogens, Journal Year: 2023, Volume and Issue: 19(5), P. e1011397 - e1011397
Published: May 22, 2023
Mycoviruses are widely present in all major groups of fungi but those entomopathogenic Metarhizium spp. remain understudied. In this investigation, a novel double-stranded (ds) RNA virus is isolated from majus and named partitivirus 1 (MmPV1). The complete genome sequence MmPV1 comprises two monocistronic dsRNA segments (dsRNA 2), which encode an RNA-dependent polymerase (RdRp) capsid protein (CP), respectively. classified as new member the genus Gammapartitivirus family Partitiviridae based on phylogenetic analysis. As compared to MmPV1-free strain, isogenic MmPV1-infected single-spore isolates were compromised terms conidiation, tolerance heat shock UV-B irradiation, while these phenotypes accompanied by transcriptional suppression multiple genes involved response DNA damage repair. attenuated fungal virulence since infection resulted reduced hydrophobicity, adhesion, cuticular penetration. Additionally, secondary metabolites significantly altered infection, including production triterpenoids, metarhizins A B, increased nitrogen phosphorus compounds. However, expression individual proteins M. had no impact host phenotype, suggesting insubstantive links between defective single viral protein. These findings indicate that decreases fitness its environment insect-pathogenic lifestyle through orchestration stress tolerance, pathogenicity, metabolism.
Language: Английский
Citations
24
Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 75, P. 102337 - 102337
Published: June 19, 2023
Language: Английский
Citations
24
iScience, Journal Year: 2023, Volume and Issue: 26(6), P. 106793 - 106793
Published: May 3, 2023
Investigation of fungal biology has been frequently motivated by the fact that many species are important plant and animal pathogens. Such efforts have contributed significantly toward our understanding pathogenic lifestyles (virulence factors strategies) interplay with host immune systems. In parallel, work on allorecognition systems leading to characterization regulated cell death determinants pathways, instrumental for emergent concept immunity. The uncovered evolutionary
Language: Английский
Citations
23
Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(12)
Published: March 14, 2024
The presence of viruses that spread to both plant and fungal populations in nature has posed intriguingly scientific question. We found a negative-strand RNA virus related members the family Phenuiviridae , named Valsa mali 1 (VmNSRV1), which induced strong hypovirulence was prevalent population phytopathogenic fungus apple canker ( ) infecting orchards Shaanxi Province China. Intriguingly, VmNSRV1 encodes protein with viral cell-to-cell movement function tissue. Mechanical leaf inoculation showed could systemically infect plants. Moreover, detected 24 out 139 trees tested Province. Fungal experiments be bidirectionally transmitted between plants V. mali, infection reduced development lesions on leaves. Additionally, nucleocapsid encoded by is associated rearranged lipid droplets cells. represents adapted hosts shuttles these two organisms (phyto-mycovirus) potential utilized for biocontrol method against diseases. This finding presents further insights into evolution adaptation encompassing hosts.
Language: Английский
Citations
14
Molecular Plant, Journal Year: 2024, Volume and Issue: 17(6), P. 955 - 971
Published: May 13, 2024
Circular single-stranded DNA (ssDNA) viruses have been rarely found in fungi, and the evolutionary ecological relationships among ssDNA infecting fungi other organisms remain unclear. In this study, a novel circular virus, tentatively named Diaporthe sojae virus 1 (DsCDV1), was identified phytopathogenic fungus isolated from pear trees. DsCDV1 has monopartite genome (3185 nt size) encapsidated isometric virions (21-26 nm diameter). The comprises seven putative open reading frames encoding discrete replicase (Rep) split by an intergenic region, capsid protein (CP), several proteins of unknown function (P1-P4), long region. Notably, two parts Rep share high identities with Reps Geminiviridae Genomoviridae, respectively, indicating linkage both families. Phylogenetic analysis based on or CP sequences placed unique cluster, supporting establishment new family, Gegemycoviridae, intermediate to significantly attenuates fungal growth nearly erases virulence when transfected into host fungus. Remarkably, can systematically infect tobacco seedlings, providing broad-spectrum resistance diseases. Subcellular localization revealed that P3 is localized plasmodesmata, while its expression trans-complementation experiments could restore systematic infection movement-deficient plant suggesting movement protein. exhibits molecular biological traits not observed viruses, serving as link between presenting connection fungi. These findings contribute expanding our understanding diversity evolution, offering potential biocontrol applications for managing crucial
Language: Английский
Citations
12
Current Forestry Reports, Journal Year: 2024, Volume and Issue: 10(3), P. 153 - 174
Published: Feb. 21, 2024
Abstract Purpose of Review The collective virome forest trees can be considered to include not only plant viruses, but also viral communities harbored by all tree-associated organisms. In this review, we will concentrate on reviewing recent developments in the two fields tree virology that have received most research input during last 5 years: (1) current knowledge virus diseases affecting and their causal agents (2) fungal viruses (mycoviruses) properties are required for utilizing them biocontrol purposes. Recent Findings discovery mycoviruses has been revolutionized few years due high-throughput sequencing (HTS). This altered our view diversity prevalence, host ranges, transmission routes, effects. Utilization HTS greatly expanded disease etiology revealed commonness cross-kingdom events between fungi, oomycetes, plants, arthropods. Research identified several new restrict growth or virulence pathogenic fungi. Summary Gaining ecosystems is essential understanding evolution improving impacts, ability biocontrol-based environmentally friendly management affect economically important plants beneficial insects, preventing possible outbreaks future. Virus infections play a central role health, symptoms remain often unrecognized may confused with other biotic abiotic damages. However, studies previously unknown as causes suggest responsible far greater economic losses than recognized earlier. many gaps still need filled, particularly infect different species trees, irregular distribution within plant, mode transmission, epidemiology choice hosts regarding crop effect metabolism tree, interaction microorganisms. Mycovirus already deciphered detailed information critical Still, more needed concerning mycoviral stability field conditions, level tolerance against infection, occurrence interspecies mycovirus nature, safety issues related these topics.
Language: Английский
Citations
10