Inhibition of GSK-3β Restores Differentiation Potential of Late-Passage Mesenchymal Stem Cells DOI Creative Commons
Kavitha Govarthanan, Raja Sundari Meenakshi Sundaram, Arthi Sunil Richard

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(4), P. 483 - 483

Published: March 28, 2025

Background/Objectives: Mesenchymal stem cells (MSCs) are regarded as a promising cell type with significant therapeutic benefits owing to their ease of isolation, maintenance, and characterisation. However, repeated passages during cultural maintenance frequently result in cellular senescence, limiting utility regenerative medicine. Methods: We investigated the differentiation capability between early- (P3) late-passage MSCs (>P15) tested potential Wnt agonist 99021 reverse using standard culture protocols that define minimal criteria for MSCs, primarily tri-lineage assays, biochemical staining gene expression analysis, senescence assays. Results: initially noticed distinct signs morphological aging further examined (>P15). found diminished MSCs. Our assay also revealed >P15 were able absorb dye, indicating underwent senescence. demonstrated CHIR reversed inhibitory potential-mediated impasse by employing specific labelling, analysis. Senescence assays after treatment no dye uptake at all. Conclusions: findings maybe aids reversal MSC aging-related inhibition glitches offers proven protocol rejuvenating Thus, CHIR99021 inherently reverts potency this method could be employed ensure plentiful source clinical purposes.

Language: Английский

Mesenchymal stromal cells for improvement of cardiac function following acute myocardial infarction: a matter of timing DOI
Stéphanie Barrère‐Lemaire, Anne Vincent, Christian Jørgensen

et al.

Physiological Reviews, Journal Year: 2023, Volume and Issue: 104(2), P. 659 - 725

Published: Aug. 17, 2023

Acute myocardial infarction (AMI) is the leading cause of cardiovascular death and remains most common heart failure. Reopening occluded artery, i.e., reperfusion, only way to save myocardium. However, expected benefits reducing infarct size are disappointing due reperfusion paradox, which also induces specific cell death. These ischemia-reperfusion (I/R) lesions can account for up 50% final size, a major determinant both mortality risk failure (morbidity). In this review, we provide detailed description inflammation mechanisms as features I/R injury cardioprotective strategies such ischemic postconditioning well their underlying mechanisms. Due biological properties, use mesenchymal stromal/stem cells (MSCs) has been considered potential therapeutic approach in AMI. Despite promising results evidence safety preclinical studies using MSCs, effects reported clinical trials not conclusive even inconsistent. discrepancies were attributed many parameters donor age, vitro culture, storage time injection window after AMI, alter MSC properties. context future directions will be generate MSCs with enhanced properties limit tissue thereby reduce improve healing phase increase postinfarct performance.

Language: Английский

Citations

36
Mesenchymal Stromal Cells as a Driver of Inflammaging DOI Open Access
Svetlana Lyamina, Denis Baranovskii,

Ekaterina Kozhevnikova

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(7), P. 6372 - 6372

Published: March 28, 2023

Life expectancy and age-related diseases burden increased significantly over the past few decades. Age-related conditions are commonly discussed in a very limited paradigm of depleted cellular proliferation maturation with exponential accumulation senescent cells. However, most recent evidence showed that majority age-associated ailments, i.e., diabetes mellitus, cardiovascular neurodegeneration. These closely associated tissue nonspecific inflammation triggered controlled by mesenchymal stromal cell secretion. Mesenchymal cells (MSCs) known as common type for therapeutic approaches clinical practice. Side effects complications MSC-based treatments interest MSCs secretome an alternative concept validation tests regenerative medicine. The data also proposed it ideal tool cell-free therapy engineering. was shown to hold role ‘key-driver’ inflammaging. We aimed review immunomodulatory MSCs-secretome during senescence provide eventual insight into interpretation its beneficial biological actions inflammaging-associated diseases.

Language: Английский

Citations

30
Regulation Mechanisms and Maintenance Strategies of Stemness in Mesenchymal Stem Cells DOI

Nizhou Jiang,

Xiliang Tian, Quanxiang Wang

et al.

Stem Cell Reviews and Reports, Journal Year: 2023, Volume and Issue: 20(2), P. 455 - 483

Published: Nov. 27, 2023

Language: Английский

Citations

23
Iron-Mediated Regulation in Adipose Tissue: A Comprehensive Review of Metabolism and Physiological Effects DOI
Xinyu Yang, Xianghong Wang, Zhe Yang

et al.

Current Obesity Reports, Journal Year: 2025, Volume and Issue: 14(1)

Published: Jan. 3, 2025

Language: Английский

Citations

1
Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process DOI Creative Commons
Wei Zheng, Xu Wang, Yaqin Li

et al.

Cellular & Molecular Biology Letters, Journal Year: 2022, Volume and Issue: 27(1)

Published: Oct. 8, 2022

Research on aging is growing as the elderly make up a greater share of population, focusing reversing and inhibiting process. The exhaustion senescence stem cells are fundamental drivers behind aging. β-Carotene has been depicted to have many biological functions, we speculate that it may an anti-aging effect.Firstly, property β-carotene was investigated in vitro using mesenchymal (MSCs) induced by H2O2. effect characterized Western-bloting, confocal laser scanning microscopy, indirect immunofluorescence, immunohistochemistry. also tested vivo aged mice.The experiment revealed could relieve MSCs, evidenced series marker molecules such p16 p21. appeared inhibit regulating KAT7-P15 signaling axis. treatment significantly down-regulated level tissues organs.In this work, explored vitro. experimental results indicate be important potential molecule, which can used drug or functional food treat future.

Language: Английский

Citations

28
Mesenchymal stem cells under epigenetic control – the role of epigenetic machinery in fate decision and functional properties DOI Creative Commons
Alicja Walewska, Adrian Janucik, Marlena Tynecka

et al.

Cell Death and Disease, Journal Year: 2023, Volume and Issue: 14(11)

Published: Nov. 6, 2023

Abstract Mesenchymal stem cells (mesenchymal stromal cells, MSC) are multipotent that can differentiate into of at least three mesodermal lineages, namely adipocytes, osteoblasts, and chondrocytes, have potent immunomodulatory properties. Epigenetic modifications critical regulators gene expression cellular differentiation mesenchymal (MSCs). machinery controls MSC through direct to DNA histones. Understanding the role epigenetic in is crucial for development effective cell-based therapies degenerative inflammatory diseases. In this review, we summarize current understanding control

Language: Английский

Citations

18
Enhancement of the therapeutic efficacy of mesenchymal stem cell-derived exosomes in osteoarthritis DOI Creative Commons
Zehao Zhang, Sheng Zhao,

Zhaofeng Sun

et al.

Cellular & Molecular Biology Letters, Journal Year: 2023, Volume and Issue: 28(1)

Published: Sept. 28, 2023

Abstract Osteoarthritis (OA), a common joint disorder with articular cartilage degradation as the main pathological change, is major source of pain and disability worldwide. Despite current treatments, overall treatment outcome unsatisfactory. Thus, patients severe OA often require replacement surgery. In recent years, mesenchymal stem cells (MSCs) have emerged promising therapeutic option for preclinical clinical palliation OA. MSC-derived exosomes (MSC-Exos) carrying bioactive molecules parental cells, including non-coding RNAs (ncRNAs) proteins, demonstrated significant impact on modulation various physiological behaviors in cavity, making them candidates cell-free therapy This review provides comprehensive overview biosynthesis composition MSC-Exos their mechanisms action We also discussed potential tool modulating intercellular communication Additionally, we explored bioengineering approaches to enhance MSC-Exos’ potential, which may help overcome challenges achieve clinically meaningful therapies.

Language: Английский

Citations

13
BMP7-Loaded Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorate Liver Fibrosis by Targeting Activated Hepatic Stellate Cells DOI Creative Commons

Dan Zhu,

Zongbin Sun,

Jiayun Wei

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 3475 - 3495

Published: April 1, 2024

Purpose: Human umbilical cord mesenchymal stem cell (hucMSC)-derived small extracellular vesicles (sEVs) are natural nanocarriers with promising potential in treating liver fibrosis and have widespread applications the fields of nanomedicine regenerative medicine. However, therapeutic efficacy hucMSC-sEVs is currently limited owing to their non-specific distribution vivo partial removal by mononuclear macrophages following systemic delivery. Thus, can be improved through development engineered capable overcome these limitations. Patients Methods: To improve anti-liver hucMSC-sEVs, we genetically overexpress anti-fibrotic gene bone morphogenic protein 7 ( BMP7 ) parental cells. This was achieved using lentiviral transfection, which BMP7-loaded were isolated ultracentrifugation. First, induced C57BL/6J mice intraperitoneal injection 50% carbon tetrachloride (CCL4) twice a week for 8 weeks. These subsequently treated BMP7+sEVs via tail vein injection, effect validated animal imaging, immunohistochemistry (IHC), tissue immunofluorescence, enzyme-linked immunosorbent assay (ELISA). Finally, function studies performed confirm results. Results: Liver imaging histopathology confirmed that could reach aggregate around activated hepatic stellate cells (aHSCs) significantly stronger compared those blank or negative control-transfected hucMSC-sEVs. In vitro, promoted phenotypic reversal aHSCs inhibited proliferation enhance effects. Conclusion: offer novel strategy clinical treatment fibrosis. Keywords: hucMSCs, sEVs, aHSCs, nanocarrier,

Language: Английский

Citations

5
Amniotic Fluid-Derived Stem Cells: An Overlooked Source of Stem Cells for Translational Research DOI
Yu Jiang, Yanjiao Li, Li Duan

et al.

DNA and Cell Biology, Journal Year: 2025, Volume and Issue: 44(3), P. 144 - 152

Published: March 1, 2025

Amniotic fluid-derived stem cells (AFSCs) represent a promising yet underutilized resource for research and clinical applications. While AFSCs share phenotypic functional characteristics with derived from somatic tissues such as bone marrow, adipose tissue, placenta, umbilical cord, their unique developmental origin grants them several superior qualities. These include enhanced multipotency, tissue-specific genotypic profiles, the ability to form single-cell colonies. Such features position highly valuable translational tissue engineering. This review seeks underscore distinctive attributes of AFSCs, particularly relevance in By emphasizing these qualities, we aim stimulate further exploration into use patient-specific induced pluripotent organoid development, potentially unlocking full therapeutic potential. The capabilities make an exceptional candidate advancing regenerative medicine, offering new avenues treating variety conditions that currently have limited options.

Language: Английский

Citations

0
Senescence induces fundamental changes in the secretome of mesenchymal stromal cells (MSCs): implications for the therapeutic use of MSCs and their derivates DOI Creative Commons
Yesuf Adem Siraj, Umberto Galderisi, Nicola Alessio

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: May 9, 2023

Mesenchymal stromal cells (MSCs) are a heterogeneous population containing multipotent adult stem with multi-lineage differentiation capacity, which differentiated into mesodermal derivatives. MSCs employed for therapeutic purposes and several investigations have demonstrated that the positive effects of MSC transplants due to capacity modulate tissue homeostasis repair via activity their secretome. Indeed, MSC-derived secretomes now an alternative strategy cell transplantation anti-inflammatory, anti-apoptotic, regenerative effects. The cellular senescence is dynamic process leads permanent cycle arrest, loss healthy cells’ physiological functions acquiring new activities, mainly accrued through release many factors, indicated as senescence-associated secretory phenotype (SASP). occurring in cells, such those present MSCs, may detrimental on health since it can undermine repair. analysis secretome important either use main mechanism activity, loses its beneficial acquire negative pro-inflammatory pro-aging activities when become senescent. When or derivatives planned be used purposes, great attention must paid these changes. In this review, we analyzed changes following switch from status.

Language: Английский

Citations

12