Mesenchymal stem cell-derived extracellular vesicles in skin wound healing: roles, opportunities and challenges

Military Medical Research, Journal Year: 2023, Volume and Issue: 10(1)

Published: Aug. 17, 2023

Abstract Skin wounds are characterized by injury to the skin due trauma, tearing, cuts, or contusions. As such injuries common all human groups, they may at times represent a serious socioeconomic burden. Currently, increasing numbers of studies have focused on role mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) in wound repair. cell-free therapy, MSC-derived EVs shown significant application potential field repair as more stable and safer option than conventional therapy. Treatment based can significantly promote damaged substructures, including regeneration vessels, nerves, hair follicles. In addition, inhibit scar formation affecting angiogenesis-related antifibrotic pathways promoting macrophage polarization, angiogenesis, proliferation, migration, inhibiting excessive matrix production. Additionally, these structures serve scaffold for components used repair, be developed into bioengineered support trauma Through formulation standardized culture, isolation, purification, drug delivery strategies, exploration detailed mechanism will allow them clinical treatments conclusion, EVs-based therapies important prospects Here we provide comprehensive overview their current status, potential, associated drawbacks.

Language: Английский

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Application of extracellular vesicles derived from mesenchymal stem cells as potential therapeutic tools in autoimmune and rheumatic diseases

International Immunopharmacology, Journal Year: 2022, Volume and Issue: 106, P. 108634 - 108634

Published: Feb. 19, 2022

Language: Английский

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Applications of mesenchymal stem cell-exosome components in wound infection healing: new insights

Burns & Trauma, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 1, 2024

Abstract The healing process at a wound is made up of many types cells, growth factors, the extracellular matrix, nerves and blood vessels all interacting with each other in complex changing ways. Microbial colonization proliferation are possible place injury, which makes infection more likely. Because this, any cut has chance getting an infection. Researchers have found that infections make patients upset cost healthcare system lot money. Surgical site happen to people who recently had surgery. This study shows such surgical linked high rate illness death. shown by fact 25% get serious sepsis need be transferred intensive care unit. In both animal models people, mesenchymal stem cells (MSCs) play active role stages positive effects. Exosomes one main things MSCs release. They effects similar those parent MSCs. Various effector proteins, messenger RNA microRNAs can transported vesicles control activity target cells. big impact on process. These results suggest using MSC-exosomes as new type cell-free therapy could better safer option than whole cell therapy. review mostly about how use parts help heal.

Language: Английский

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Understanding exosomes: Part 2—Emerging leaders in regenerative medicine

Periodontology 2000, Journal Year: 2024, Volume and Issue: 94(1), P. 257 - 414

Published: Feb. 1, 2024

Abstract Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with ability to communicate other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due their be utilized as therapeutic options for a wide array diseases/conditions. Over 5000 publications currently being published yearly on this topic, number is only expected dramatically increase novel strategies continue developed. Today exosomes have been applied numerous contexts including neurodegenerative disorders (Alzheimer's disease, central nervous system, depression, multiple sclerosis, Parkinson's post‐traumatic stress disorders, traumatic brain injury, peripheral nerve injury), damaged organs (heart, kidney, liver, stroke, myocardial infarctions, ovaries), degenerative processes (atherosclerosis, diabetes, hematology musculoskeletal degeneration, osteoradionecrosis, respiratory disease), infectious diseases (COVID‐19, hepatitis), procedures (antiaging, bone regeneration, cartilage/joint osteoarthritis, cutaneous wounds, dental dermatology/skin erectile dysfunction, hair regrowth, intervertebral disc repair, spinal cord vascular regeneration), cancer therapy (breast, colorectal, gastric osteosarcomas), immune function (allergy, autoimmune regulation, inflammatory diseases, lupus, rheumatoid arthritis). This scoping review first its kind aimed at summarizing extensive potential broad range disorders.

Language: Английский

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circCDK13-loaded small extracellular vesicles accelerate healing in preclinical diabetic wound models

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 9, 2024

Abstract Chronic wounds are a major complication in patients with diabetes. Here, we identify therapeutic circRNA and load it into small extracellular vesicles (sEVs) to treat diabetic preclinical models. We show that circCDK13 can stimulate the proliferation migration of human dermal fibroblasts epidermal keratinocytes by interacting insulin-like growth factor 2 mRNA binding protein 3 an N6-Methyladenosine-dependent manner enhance CD44 c-MYC expression. engineered sEVs overexpress local subcutaneous injection male db/db mouse streptozotocin-induced type I rats could accelerate wound healing skin appendage regeneration. Our study demonstrates delivery may present option for treatment.

Language: Английский

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An injectable, activated neutrophil-derived exosome mimetics/extracellular matrix hybrid hydrogel with antibacterial activity and wound healing promotion effect for diabetic wound therapy

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Aug. 30, 2023

Chronic diabetic wounds are primarily caused by infection, inflammation, and angiogenesis-related disorders. An ideal approach for treating chronic is combining anti-infection strategies, immune microenvironment regulation, angiogenesis promotion. Vascular endothelial growth factor (VEGF) can promote the proliferation migration of vascular cells, thereby promoting angiogenesis. However, low stability inability to target lesions limit its application. Polymorphonuclear neutrophil-derived exosomes (PMNExo) exhibit good delivery properties be used therapeutic VEGF. Furthermore, they retain antibacterial ability polymorphonuclear neutrophils (PMNs). Nonetheless, PMNExo generation impedes applications. In this study, we prepared exosome mimetics (EM) from PMNs using extrusion process; as a result, yield significantly improved. To increase residence exosomes, an extracellular matrix (ECM) hydrogel, thermosensitive material that function in situ gel vivo, was carrier. The active peptides ECM regulated wound. summary, loaded with VEGF-encapsulated activated neutrophil (aPMNEM) develop VEGF-aPMNEM-ECM hybrid hydrogel wounds. accelerates regeneration Our study provides prospective therapy platform involving cytokines different diseases.

Language: Английский

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The functions and clinical application potential of exosomes derived from mesenchymal stem cells on wound repair: a review of recent research advances

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 25, 2023

Wound repair is a complex problem for both clinical practitioners and scientific investigators. Conventional approaches to wound have been associated with several limitations, including prolonged treatment duration, high expenses, significant economic psychological strain on patients. Consequently, there pressing demand more efficacious secure modalities enhance the existing landscapes. In field of repair, cell-free therapy, particularly use mesenchymal stem cell-derived exosomes (MSC-Exos), has made notable advancements in recent years. Exosomes, which are small lipid bilayer vesicles discharged by MSCs, harbor bioactive constituents such as proteins, lipids, microRNA (miRNA), messenger RNA (mRNA). These facilitate material transfer information exchange between cells, thereby regulating their biological functions. This article presents comprehensive survey function mechanisms MSC-Exos context healing, emphasizing beneficial impact each phase process, regulation immune response, inhibition inflammation, promotion angiogenesis, advancement cell proliferation migration, reduction scar formation.

Language: Английский

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Adipose stem cells in tissue regeneration and repair: From bench to bedside

Regenerative Therapy, Journal Year: 2023, Volume and Issue: 24, P. 547 - 560

Published: Oct. 11, 2023

ADSCs are a large number of mesenchymal stem cells in Adipose tissue, which can be applied to tissue engineering. have the potential multi-directional differentiation, and differentiate into bone cardiac urothelial cells, skin etc. Compared with other multitude promising advantages, such as abundant number, accessibility cell culture, stable function, less immune rejection. There two main methods use for repair regeneration. One is implant "ADSCs-scaffold composite" injured site promote The cell-free therapy: using ADSC-exos or ADSC-CM alone release miRNAs, cytokines bioactive substances regeneration regulated by variety cytokines, signaling molecules, external environment. differentiation different tissues also induced growth factors, ions, hormones, scaffold materials, physical stimulation, factors. specific mechanisms complex, most pathways need further explored. This article reviews summarizes mechanism clinical application injury so far, puts forward problems that solved this field, hoping provide directions research field.

Language: Английский

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The effectiveness of cell-derived exosome therapy for diabetic wound: A systematic review and meta-analysis

Ageing Research Reviews, Journal Year: 2023, Volume and Issue: 85, P. 101858 - 101858

Published: Jan. 18, 2023

The prevalence of diabetes among the elderly population is significant and rising annually. One most severe frequent complications mellitus diabetic wound, which has long-term negative effects on patients' finances, mental health, functional abilities. Exosomes, a cell-free therapy, have emerged as promising novel treatment for wounds, but their mechanism still not entirely understood. Therefore, we conducted this meta-analysis to assess effectiveness exosomes in management wounds.We searched PubMed, Cochrane Library, EMBASE, Web Science pertinent studies that described therapeutic benefits wound models were released before October 17, 2022. outcome indicators consisted healing rate, neovascular density, re-epithelialization collagen deposition, scar width, inflammatory factors. RevMan 5.4 software was used conduct all statistical analyses.A total 21 with 323 animals identified meta-analysis. Pooled analyses demonstrated exosome therapy shown be superior control terms rate (SMD = 5.42; 95 %CI 4.40-6.44; P < 0.00001), density 5.48; 4.31-6.64; 5.06; 3.75-6.37; deposition 4.78; 3.58-5.98; width -8.10; -10.31 -5.89; 0.00001). Additionally, expression factors significantly downregulated group.According current trials, can enhance quality especially when conjunction dressings. To demonstrate efficient parameters future should sizable, randomized, double-blind trials high-quality, follow-ups.

Language: Английский

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Recombinant human collagen I/carboxymethyl chitosan hydrogel loaded with long-term released hUCMSCs derived exosomes promotes skin wound repair

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130843 - 130843

Published: March 12, 2024

Language: Английский

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