Microbiota-derived indoles alleviate intestinal inflammation and modulate microbiome by microbial cross-feeding

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: March 19, 2024

Abstract Background The host–microbiota interaction plays a crucial role in maintaining homeostasis and disease susceptibility, microbial tryptophan metabolites are potent modulators of host physiology. However, whether how these mediate interactions, particularly terms inter-microbial communication, remains unclear. Results Here, we have demonstrated that indole-3-lactic acid (ILA) is key molecule produced by Lactobacillus protecting against intestinal inflammation correcting dysbiosis. Specifically, metabolizes into ILA, thereby augmenting the expression bacterial enzymes implicated metabolism, leading to synthesis other indole derivatives including indole-3-propionic (IPA) indole-3-acetic (IAA). Notably, IPA, IAA possess ability mitigate modulate gut microbiota both DSS-induced IL-10 −/− spontaneous colitis models. ILA increases abundance tryptophan-metabolizing bacteria (e.g., Clostridium ), as well mRNA acyl-CoA dehydrogenase indolelactate vivo vitro, resulting an augmented production IPA IAA. Furthermore, mutant strain fails protect producing derivatives. ILA-mediated cross-feeding was microbiota-dependent specifically enhanced under conditions dysbiosis induced Citrobacter rodentium or DSS, but not disruption with antibiotics. Conclusion Taken together, highlight mechanisms which microbiome-host crosstalk cooperatively control homoeostasis through microbiota-derived indoles mediating communication. These findings may contribute development targeted “postbiotic” potential interventions for treatment prevention dysbiosis-driven diseases.

Language: Английский

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Lactiplantibacillus plantarum enables blood urate control in mice through degradation of nucleosides in gastrointestinal tract

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: July 19, 2023

Abstract Background Lactobacillus species in gut microbiota shows great promise alleviation of metabolic diseases. However, little is known about the molecular mechanism how interacts with metabolites circulation. Here, using high nucleoside intake to induce hyperuricemia mice, we investigated improvement systemic urate metabolism by oral administration L. plantarum via different host pathways. Results Gene expression analysis demonstrated that inhibited activity xanthine oxidase and purine phosphorylase liver suppress synthesis. The composition did not dramatically change over 14 days, indicated no significant difference α β diversities. multi-omic network revealed increase decrease johnsonii contributed a serum levels. Besides, genomic recombinant protein showed three ribonucleoside hydrolases, RihA–C, rapidly cooperatively catalyzed hydrolysis nucleosides into nucleobases. Furthermore, absorption nucleobase intestinal epithelial cells was less than nucleoside, which resulted reduction generation, evidenced phenomenon mice fed diet generated those period 9-day gavage. Conclusion Collectively, our work provides substantial evidence identifying specific role We highlight importance enzymes RihA–C existing for induced high-nucleoside diet. Although direct connection between transport levels has been identified, lack transporter might be important its metabolization production, leading host. These findings provide insights regulation.

Language: Английский

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Maternal consumption of l-malic acid enriched diets improves antioxidant capacity and glucose metabolism in offspring by regulating the gut microbiota

Redox Biology, Journal Year: 2023, Volume and Issue: 67, P. 102889 - 102889

Published: Sept. 19, 2023

Maternal diets during pregnancy and lactation are key determinants that regulate the development of metabolic syndrome (MetS) in offspring. l-malic acid (MA) was previously reported to improve antioxidant capacity aerobic metabolism. However, effects maternal MA consumption on features offspring remain largely unexplored. Herein, through pig models consuming MA-enriched late lactation, we found potentiated anti-inflammatory sows, thereby improving their reproductive performance growth piglets. also induced a transition slow-twitch fast-twitch fibers early life Along with muscle fiber-type transition, insulin sensitivity glucose metabolism, including metabolism glycolysis, were improved skeletal An untargeted metabolomic analysis further revealed contribution modified amino Mechanistically, remodeled colonic microbiota Briefly, abundance Colidextribacter, Romboutsia, Family_XIII_AD3011_group increased, which positively associated muscles. A decreased Prevotella, Blautia, Prevotellaceae_NK3B31_group, Collinsella detected, involved less sensitivity. Notably, milk metabolites, such as ascorbic (AA) granisetron (GS), effectors regulating gut composition The properties AA GS alleviating resistance, inflammation, oxidative stress verified mice treated high-fat diets. Overall, this study could modulate inflammatory response, capacity, by vertical transmission metabolites. These findings suggest potential prevention treatment MetS life.

Language: Английский

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Maternal Malic Acid May Ameliorate Oxidative Stress and Inflammation in Sows through Modulating Gut Microbiota and Host Metabolic Profiles during Late Pregnancy

Antioxidants, Journal Year: 2024, Volume and Issue: 13(2), P. 253 - 253

Published: Feb. 19, 2024

Sows suffer oxidative stress and inflammation induced by metabolic burden during late pregnancy, which negatively regulates reproductive lactating performances. We previously found that L-malic acid (MA) alleviated improved performances in sows. However, the mechanism underlying MA’s positive effects remains unexplored. Here, twenty Large White × Landrace sows with similar parity were randomly divided into two groups fed a basal diet or supplemented 2% complex from day 85 of gestation to delivery. The gut microbiome, fecal short-chain fatty acids, untargeted serum metabolome determined. Results showed Firmicutes, Bacteroidota, Spirochaetota top abundant phyla identified pregnancy for Maternal MA supplementation modulated composition but not richness diversity microbiota pregnancy. Correlation analysis between antioxidant capacity (or indicators) revealed unclassified_f_Ruminococcaceae, unclassified_f_Lachnospiraceae, UCG-002, norank_f_norank_o_RF3, Lactobacillus might play role anti-oxidation, Lachnospiraceae_XPB1014_group, Lachnospiraceae_NK4A136_group, Candidatus_Soleaferrea, norank_f_UCG-010, norank_f_norank_o_RF39, unclassified_f_Lachnospiraceae be involved anti-inflammatory effect. status independent short chain changes. In addition, metabolomics exhibited different landscapes group control contribution modified amino lipid metabolism status. Notably, correlation results metabolites, as well metabolites indicators), demonstrated differential was highly related microorganisms indicators. Collectively, these data maternal dietary supply can ameliorate through modulating host profiles

Language: Английский

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Limosilactobacillus reuteri - a probiotic gut commensal with contextual impact on immunity

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 17, 2025

The gut microbiome plays a key role in human health, influencing various biological processes and disease outcomes. historical roots of probiotics are traced back to Nobel Laureate Élie Metchnikoff, who linked the longevity Bulgarian villagers their consumption sour milk fermented by Lactobacilli. His pioneering work led global recognition as beneficial supplements, now multibillion-dollar industry. Modern have been extensively studied for immunomodulatory effects. Limosilactobacillus reuteri (L. reuteri), widely used probiotic, has garnered significant attention its systemic immune-regulatory properties, particularly relation autoimmunity cancer. This review delves into L. modulating immune responses, with focus on impact diseases.

Language: Английский

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An alternative ZnO with large specific surface area: Preparation, physicochemical characterization and effects on growth performance, diarrhea, zinc metabolism and gut barrier function of weaning piglets

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 882, P. 163558 - 163558

Published: April 18, 2023

Language: Английский

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Swine Colibacillosis: Analysis of the Gut Bacterial Microbiome

Microorganisms, Journal Year: 2024, Volume and Issue: 12(6), P. 1233 - 1233

Published: June 19, 2024

This study aimed to evaluate the disruption of swine gut microbiota and histopathological changes caused by infection with enterotoxigenic E. coli. Fecal samples were collected from piglets suffering diarrhea post-recovery healthy animals. Intestinal tissues for changes. The results revealed mainly in ileum infected animals compared those control recovered operational taxonomic units (OTUs) that coli diarrheal group exhibited highest bacterial richness. Principal coordinate analysis (PCoA) corroborated presence dysbiosis following coli-induced diarrhea. While normal groups displayed slight clustering, recovery formed a distinct cluster flora. Bacteroidetes, Firmicutes, Fusobacteria dominant phyla both group. LEfSe associated LDA score dominance Euryarchaeota Bacteroidota, while N I showed Firmicutes Fusobacteriota, respectively. scores highlighted significant expression Muribaculacea family R. obtained findings will help understanding microbiome during colibacillosis, which support outbreaks.

Language: Английский

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Microcin C7 as a Potential Antibacterial-Immunomodulatory Agent in the Postantibiotic Era: Overview of Its Bioactivity Aspects and Applications

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7213 - 7213

Published: June 29, 2024

In the postantibiotic era, pathogenicity and resistance of pathogens have increased, leading to an increase in intestinal inflammatory disease. Bacterial infections remain cause animal mortality. With increasing antibiotics, there has been a significant decrease both inflammation disease animals, thus decreasing production efficiency costs. These side effects serious consequences detracted from development China's pig industry. Microcin C7 (McC7) demonstrates potent antibacterial activity against broad spectrum pathogens, stable physicochemical properties, low toxicity, reducing likelihood development. Thus, McC7 received attention as potential clinical immunomodulatory agent. serve new generation antibiotic substitutes; however, its commercial applications livestock poultry industry limited. this review, we summarize discuss biosynthesis, biochemical structural characteristics, mechanism action, immune strategies McC7. We also describe ability improve health. Our aim study was provide theoretical basis for application feed additive or veterinary drug breeding industry, providing strategy alleviating through mitigating resistance. Furthermore, review provides insight into functions anti-infection mechanisms bacteriocin peptides proposes crucial ideas research, product development, different fields, such food medical industries.

Language: Английский

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Effects of Dietary Supplementation with Lactobacillus reuteri Postbiotics on Growth Performance, Intestinal Flora Structure and Plasma Metabolome of Weaned Piglets

Animals, Journal Year: 2025, Volume and Issue: 15(2), P. 204 - 204

Published: Jan. 14, 2025

Probiotics and their postbiotics have the potential to improve health growth performance of piglets, which has brought them widespread attention in post-antibiotic era. In present study, effects dietary supplementation Lactobacillus reuteri on performance, intestinal flora structure plasma metabolome weaned piglets were investigated. A total 816 healthy male with uniform weight divided into two treatment groups: control (CTR) group fed a basic diet, ones LAC diet supplemented 500 mg/kg postbiotics. There six replicates each 68 replicate. The animal trial lasted for 30 days. feces blood collected investigation, during was counted. Our outcomes show that had no effect piglets; however, it reduced mortality rate by 6.37%. levels superoxide dismutase serum, propionic acid butyric elevated, content malondialdehyde serum decreased postbiotics-treated (p < 0.05). fecal sequencing results relative abundance Firmicutes monoglobus upregulated, Bacteroides downregulated addition, positively correlated negatively raised level coenzyme Q10 serum. conclusion, contributed improving antioxidant function reducing regulating upregulating

Language: Английский

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Maternal Gut Inflammation Aggravates Acute Liver Failure Through Facilitating Ferroptosis via Altering Gut Microbial Metabolism in Offspring

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Abstract Microbial transmission from mother to infant is important for offspring microbiome formation and health. However, it unclear whether maternal gut inflammation (MGI) during lactation influences mother‐to‐infant microbial microbiota disease susceptibility. In this study, found that MGI altered the of suckling pups by shaping in mammary glands. MGI‐induced changes lasted into adulthood, resulting exacerbation acute liver failure (ALF) caused acetaminophen (APAP) offspring. Specifically, reduced abundance Lactobacillus reuteri ( L. ) its metabolite indole‐3‐acetic acid (IAA) level adult IAA alleviated ALF mice promoting intestinal IL‐22 production. Mechanistically, limits APAP‐induced excessive oxidative stress ferroptosis activating STAT3. The abundances are inversely associated with progression patients ALF. Overall, study reveals role development offspring, highlighting potential strategies intervention based on IAA‐IL‐22‐STAT3 axis.

Language: Английский

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