Garlic-Derived Exosome-Like Nanoparticles Enhance Gut Homeostasis in Stressed Piglets: Involvement of Lactobacillus reuteri Modulation and Indole-3-propionic Acid Induction DOI
Xiyuan Liu,

Xinyu Zhang,

Haozhen Liu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

The occurrence of pediatric diarrhea is frequently associated with inflammatory responses, compromised barrier function, and dysbiosis in the gut. These conditions are commonly triggered by stressors, similar to postweaning observed piglets. Garlic-derived exosome-like nanoparticles (GELNs) hold potential for ameliorating stress-induced diarrhea, yet supporting evidence remains scarce. Following successful isolation GELNs, this study employed weaned piglets as a model evaluate regulatory effects GELNs on intestinal integrity, mucosal inflammation, gut microbiota its metabolites. Weaned Bama miniature were orally administered phosphate buffer saline (PBS) or 1 week later, samples collected following slaughter. Histological molecular biological techniques performed examine structure, tight junction protein expression, mucin secretion, T lymphocyte infiltration, levels pro-inflammatory cytokines. composition was analyzed using 16S rRNA sequencing, while derived metabolites profiled via untargeted metabolomics. Subsequently, correlation analyses associations between metabolites, well key indicators function cytokine response GELNs. isolated exhibit typical exosome characteristics size morphology, alongside rich content proteins RNAs. incidence reduced supplementation at dosage 50 mg/kg body weight, compared control group. In addition, receiving displayed an increase within tissues jejunum, ileum, colon, decrease CD8+ counts suppression cytokines (IL-8 TNF-α) layers both jejunum ileum. Furthermore, sequencing unveiled that reshaped colonic augmenting beneficial bacteria, notably Lactobacillus reuteri, correlating strongly diminished TNF-α heightened expression. Metabolite analysis demonstrated significant indole-3-propionic acid, from microbiota, supplemented This positively correlated abundance reuteri negatively linked IL-8 summary, our demonstrates mitigate stress-related inflammation enhance production piglets, which potentially achieved through optimization composition, specifically increasing induction anti-inflammatory microbial metabolite acid. findings presented here provide essential groundwork future development therapeutic strategy aimed enhancing homeostasis disruption caused stress children.

Language: Английский

Identification of the key tryptophan metabolic characteristics of Lactiplantibacillus plantarum for aryl hydrocarbon receptor activation and ulcerative colitis alleviation DOI

Xuemei Zuo,

Tingting Zhang, Xueqian Dong

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 203, P. 115766 - 115766

Published: Jan. 23, 2025

Language: Английский

Citations

2
Exploring the Role of the Gut Microbiota in Modulating Colorectal Cancer Immunity DOI Creative Commons
Nikolay K. Shakhpazyan, Л.М. Михалева, A.L. Bedzhanyan

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(17), P. 1437 - 1437

Published: Aug. 27, 2024

The gut microbiota plays an essential role in maintaining immune homeostasis and influencing the landscape within tumor microenvironment. This review aims to elucidate interactions between dynamics, with a focus on colorectal cancer (CRC). spans foundational concepts of immuno-microbial interplay, factors microbiome composition, evidence linking immunotherapy outcomes. Gut modulates anti-cancer immunity through several mechanisms, including enhancement surveillance modulation inflammatory responses. Specific microbial species their metabolic byproducts can significantly influence efficacy immunotherapies. Furthermore, diversity correlates clinical outcomes CRC, suggesting potential as valuable biomarker for predicting response immunotherapy. Conclusions: Understanding relationship responses offers novel therapeutic strategies development. not only influences natural history treatment CRC but also serves critical modulator activity. Further exploration into microbiome's could enhance effectiveness existing treatments guide development new modalities.

Language: Английский

Citations

10
Ecological dynamics of Enterobacteriaceae in the human gut microbiome across global populations DOI Creative Commons
Qi Yin,

A. C. da Silva,

Francisco Zorrilla

et al.

Nature Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 10, 2025

Abstract Gut bacteria from the Enterobacteriaceae family are a major cause of opportunistic infections worldwide. Given their prevalence among healthy human gut microbiomes, interspecies interactions may play role in modulating infection resistance. Here we uncover global ecological patterns linked to colonization and abundance by leveraging large-scale dataset 12,238 public metagenomes spanning 45 countries. Machine learning analyses identified robust microbiome signature associated with status, consistent across health states geographic locations. We classified 172 microbial species as co-colonizers 135 co-excluders, revealing genus-wide signal resistance within Faecalibacterium strain-specific co-colonization underexplored Faecalimonas phoceensis . Co-exclusion is functions involved short-chain fatty acid production, iron metabolism quorum sensing, while greater functional diversity metabolic resemblance Enterobacteriaceae. Our work underscores critical intestinal environment success gut-associated pathogens implications for developing non-antibiotic therapeutic strategies.

Language: Английский

Citations

1
Size-dependent ecotoxicological impacts of tire wear particles on zebrafish physiology and gut microbiota: implications for aquatic ecosystem health DOI
Yun Zhang, Qianqian Song,

Qingxuan Meng

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 487, P. 137215 - 137215

Published: Jan. 16, 2025

Language: Английский

Citations

1
Microbial remodeling of gut tryptophan metabolism and indole-3-lactate production regulate epithelial barrier repair and viral suppression in human and simian immunodeficiency virus infections DOI Creative Commons
Clarissa Santos Rocha, Katie L. Alexander, Carolina Herrera

et al.

Mucosal Immunology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Gut inflammatory diseases cause microbial dysbiosis. Human immunodeficiency virus-1 (HIV) infection disrupts intestinal integrity, subverts repair/renewal pathways, impairs mucosal immunity and propels However, metabolic mechanisms driving repair in virally inflamed gut are not well understood. We investigated the capability of microbes to restore epithelial barriers by using a multipronged approach: an vivo simian virus (SIV)-infected nonhuman primate model HIV/AIDS, ex HIV-exposed human colorectal explants primary cells. SIV reprogrammed tryptophan (TRP) metabolism, increasing kynurenine catabolite levels that associated with barrier disruption immune suppression. Administration Lactiplantibacillus plantarum or Bifidobacterium longum subsp. infantis into SIV-inflamed lumen resulted rapid reprogramming TRP metabolism towards indole-3-lactic acid (ILA) production. This shift accelerated enhanced anti-viral defenses through induction IL-22 signaling T cells aryl hydrocarbon receptor activation. Additionally, ILA treatment tissue inhibited HIV replication reducing cytokine production cell Our findings underscore therapeutic potential TRP-to-ILA mitigating viral pathogenic effects bolstering for eradication.

Language: Английский

Citations

1
The gut microbiota-immune-brain axis: Therapeutic implications DOI Creative Commons
Kenneth J. O’Riordan, Gerard M. Moloney, Lily Keane

et al.

Cell Reports Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 101982 - 101982

Published: March 1, 2025

Language: Английский

Citations

1
The potential influence and intervention measures of gut microbiota on sperm: it is time to focus on testis-gut microbiota axis DOI Creative Commons
Wenkang Chen, Hede Zou, Haoran Xu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 8, 2024

As the global male infertility rate continues to rise, there is an urgent imperative investigate underlying causes of sustained deterioration in sperm quality. The gut microbiota emerges as a pivotal factor host health regulation, with mounting evidence highlighting its dual influence on semen. This review underscores interplay between Testis-Gut axis and consequential effects sperm. Potential mechanisms driving impact encompass immune modulation, inflammatory responses mediated by endotoxins, oxidative stress, antioxidant defenses, microbiota-derived metabolites, epigenetic modifications, regulatory sex hormone signaling. Interventions such probiotics, prebiotics, synbiotics, fecal transplantation, Traditional natural herbal extracts are hypothesized rectify dysbiosis, offering avenues modulate enhance Spermatogenesis motility. Future investigations should delve into elucidating foundational principles governing interaction within Axis. Understanding modulating Axis may yield novel therapeutic strategies fertility combat decline

Language: Английский

Citations

6
Gut microbiota determines the fate of dietary fiber-targeted interventions in host health DOI Creative Commons
Wenjing Wang,

Zhexin Fan,

Qingqing Yan

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Oct. 17, 2024

Epidemiological investigation confirmed that the intake of dietary fiber (DF) is closely related to human health, and most important factor affecting physiological function DF, besides its physicochemical properties, gut microbiota. This paper mainly summarizes interaction between DF microbiota, including influence on colonization microbiota based different role in destroying complex molecular structure by encoding carbohydrate-active enzymes, thus producing small products affect metabolism host. Taking cardiovascular disease (Atherosclerosis hypertension), liver disease, immune diseases as examples, it some such fructo-oligosaccharide, galactooligosaccharide, xylo-oligosaccharide, inulin, have prebiotic-like effects. These effects are dependent metabolites produced Therefore, this further explores how affects microbiota's production substances short-chain fatty acids, bile tryptophan metabolites, provides a preliminary explanation mechanisms associated with their impact host health. Finally, structural properties large heterogeneity composition population may be future trend utilize correlate health for precision nutrition combining information from databases.

Language: Английский

Citations

4
Probiotic, Postbiotic, and Paraprobiotic Effects of Lactobacillus rhamnosus as a Modulator of Obesity-Associated Factors DOI Creative Commons
Gabriela López-Almada, María Esther Mejía-León, Norma Julieta Salazar‐López

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(22), P. 3529 - 3529

Published: Nov. 5, 2024

Obesity is a pandemic currently affecting the world's population that decreases quality of life and promotes development chronic non-communicable diseases.

Language: Английский

Citations

4
The role of colonic microbiota amino acid metabolism in gut health regulation DOI Creative Commons
Youli Chen, Jing‐Yuan Fang

Cell Insight, Journal Year: 2025, Volume and Issue: unknown, P. 100227 - 100227

Published: Jan. 1, 2025

The human gut microbiota plays a critical role in maintaining host homeostasis through metabolic activities. Among these, amino acid (AA) metabolism by the large intestine is highly heterogeneous and relevant to health. Despite increasing interest, microbial AA remains relatively unexplored. This review highlights recent advances colonic metabolism, including auxotrophies, synthesis, dissimilatory metabolites, their implications health, focusing on major gastrointestinal diseases colorectal cancer, inflammatory bowel disease, irritable syndrome.

Language: Английский

Citations

0