The implications of single-cell RNA-seq analysis in prostate cancer: unraveling tumor heterogeneity, therapeutic implications and pathways towards personalized therapy

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: April 11, 2024

Abstract In recent years, advancements in single-cell and spatial transcriptomics, which are highly regarded developments the current era, particularly emerging integration of spatiotemporal have enabled a detailed molecular comprehension complex regulation cell fate. The insights obtained from these methodologies anticipated to significantly contribute development personalized medicine. Currently, technology is less frequently utilized for prostate cancer compared with other types tumors. Starting perspective RNA sequencing technology, this review outlined significance (scRNA-seq) research, encompassing preclinical medicine clinical applications. We summarize differences between mouse human as revealed by scRNA-seq studies, well combination multi-omics methods involving highlight key targets diagnosis, treatment, drug resistance characteristics cancer. These studies expected provide novel immunotherapy innovative treatment strategies castration-resistant Furthermore, we explore potential applications stemming technologies review, paving way future research precision

Language: Английский

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Ginsenoside Rh2 promotes hepatic stellate cell ferroptosis and inactivation via regulation of IRF1-inhibited SLC7A11

Phytomedicine, Journal Year: 2023, Volume and Issue: 118, P. 154950 - 154950

Published: July 5, 2023

Language: Английский

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Mesenchymal stem cells alleviate mouse liver fibrosis by inhibiting pathogenic function of intrahepatic B cells

Hepatology, Journal Year: 2024, Volume and Issue: unknown

Published: March 28, 2024

Background and Aims: The immunomodulatory characteristics of mesenchymal stem cells (MSCs) make them a promising therapeutic approach for liver fibrosis (LF). Here, we postulated that MSCs could potentially suppress the pro-fibrotic activity intrahepatic B cells, thereby inhibiting LF progression. Approach Results: Administration significantly ameliorated as indicated by reduced myofibroblast activation, collagen deposition, inflammation. treatment efficacy can be attributed to decreased infiltration, pro-inflammatory cytokine production cells. Single-cell RNA sequencing revealed distinct cell atlas, subtype naive (B-II) was identified, which were markedly abundant in fibrotic liver, displaying mature features with elevated expression several proliferative inflammatory genes. Transcriptional profiling total displayed proliferation, gene profile during LF. Fibrosis attenuated mice ablated (μMT) or vivo anti-CD20. Moreover, recapitulated μMT after adoptive transfer turn rescued MSC injection, validating pathogenic function on cell-promoted Mechanistically, inhibit proliferation through exosomes, regulating Mitogen-activated protein kinase Nuclear factor kappa signaling pathways. Conclusions: Intrahepatic serve target MSCs, play an important role process MSC-induced amelioration LF, may provide new clues revealing novel mechanisms action.

Language: Английский

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SKP alleviates the ferroptosis in diabetic kidney disease through suppression of HIF-1α/HO-1 pathway based on network pharmacology analysis and experimental validation

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Feb. 25, 2024

Diabetic kidney disease (DKD) represents a microvascular complication of diabetes mellitus. Shenkang Pills (SKP), traditional Chinese medicine formula, has been widely used in the treatment DKD and obvious antioxidant effect. Ferroptosis, novel mode cell death due to iron overload, shown be associated with DKD. Nevertheless, precise effects underlying mechanisms SKP on ferroptosis diabetic remain unclear.

Language: Английский

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Interferon-α stimulates DExH-box helicase 58 to prevent hepatocyte ferroptosis

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: April 15, 2024

Liver ischemia/reperfusion (I/R) injury is usually caused by hepatic inflow occlusion during liver surgery, and frequently observed war wounds trauma. Hepatocyte ferroptosis plays a critical role in I/R injury, however, it remains unclear whether this process controlled or regulated members of the DEAD/DExH-box helicase (DDX/DHX) family. The expression DDX/DHX family was screened using transcriptome analysis. Hepatocyte-specific Dhx58 knockout mice were constructed, partial operation performed. Single-cell RNA sequencing (scRNA-seq) post suggested enhanced Dhx58hep-/-. mRNAs proteins associated with DExH-box 58 (DHX58) immunoprecipitation-sequencing (RIP-seq) IP-mass spectrometry (IP-MS). Excessive production reactive oxygen species (ROS) decreased IFN-stimulated gene hepatocytes promoted ferroptosis, while treatment IFN-α increased DHX58 prevented injury. Mechanistically, RNA-binding activity constitutively associates mRNA glutathione peroxidase 4 (GPX4), central suppressor, recruits m6A reader YT521-B homology domain containing 2 (YTHDC2) to promote translation Gpx4 an m6A-dependent manner, thus enhancing GPX4 protein levels preventing ferroptosis. This study provides mechanistic evidence that stimulates m6A-modified mRNA, suggesting potential clinical application prevention

Language: Английский

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Kidney fibrosis molecular mechanisms Spp1 influences fibroblast activity through transforming growth factor beta smad signaling

iScience, Journal Year: 2024, Volume and Issue: 27(9), P. 109839 - 109839

Published: May 9, 2024

Kidney fibrosis marks a critical phase in chronic kidney disease with its molecular intricacies yet to be fully understood. This study's deep dive into single-cell sequencing data of renal tissue during pinpoints the pivotal role fibroblasts and myofibroblasts fibrotic transformation. Through identifying distinct cell populations conducting transcriptomic analysis, Spp1 emerged as key gene associated fibrosis. The experimental findings further confirm Spp1's vital function promoting fibroblast myofibroblast differentiation via TGF-β/Smad signaling pathway, underscoring contribution progression. suppression expression notably hindered this process, spotlighting promising therapeutic target for halting condensed summary encapsulates essence original research within specified word limit.

Language: Английский

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Single‐cell RNA sequencing reveals reduced intercellular adhesion molecule crosstalk between activated hepatic stellate cells and neutrophils alleviating liver fibrosis in hepatitis B virus transgenic mice post menstrual blood‐derived mesenchymal stem cell transplantation

MedComm, Journal Year: 2024, Volume and Issue: 5(8)

Published: July 22, 2024

Abstract Liver fibrosis can cause hepatitis B virus (HBV)‐associated hepatocellular carcinoma. Menstrual blood‐derived mesenchymal stem cells (MenSCs) ameliorate liver through paracrine. Single‐cell RNA sequencing (scRNA‐seq) may be used to explore the roadmap of activated hepatic stellate cell (aHSC) inactivation target fibrosis. This study established HBV transgenic (HBV‐Tg) mouse model carbon tetrachloride (CCl 4 )‐induced and demonstrated that MenSCs migrated injured improve serological indices reduce fibrotic accumulation. RNA‐bulk analysis revealed mediated extracellular matrix accumulation adhesion. parenchymal nonparenchymal were identified by scRNA‐seq in control, CCl , MenSC groups, revealing heterogeneity fibroblasts/HSCs. A CellChat diminished intercellular adhesion molecule (ICAM) signaling is vital for therapy. Specifically, Icam1 aHSCs acted on Itgal / Itgb2 Itgam neutrophils, causing decreased The expression was higher group than control after therapy neutrophil clusters. Lcn2 Pglyrp1 Wfdc21 Mmp8 had high potential targets neutrophils. highlights interacting cells, corresponding molecules, underlying treating HBV‐associated

Language: Английский

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Spatiotemporal multi-omics: exploring molecular landscapes in aging and regenerative medicine

Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)

Published: May 27, 2024

Abstract Aging and regeneration represent complex biological phenomena that have long captivated the scientific community. To fully comprehend these processes, it is essential to investigate molecular dynamics through a lens encompasses both spatial temporal dimensions. Conventional omics methodologies, such as genomics transcriptomics, been instrumental in identifying critical facets of aging regeneration. However, methods are somewhat limited, constrained by their resolution lack capacity dynamically tissue alterations. The advent emerging spatiotemporal multi-omics approaches, encompassing proteomics, metabolomics, epigenomics, furnishes comprehensive insights into intricate dynamics. These sophisticated techniques facilitate accurate delineation patterns across an array cells, tissues, organs, thereby offering in-depth understanding fundamental mechanisms at play. This review meticulously examines significance realms research. It underscores how methodologies augment our comprehension dynamics, cellular interactions, signaling pathways. Initially, delineates foundational principles underpinning methods, followed evaluation recent applications within field. ultimately concludes addressing prevailing challenges projecting future advancements Indubitably, deciphering complexities inherent regeneration, thus charting course toward potential therapeutic innovations.

Language: Английский

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Single-cell genomics reveals transcriptional heterogeneity and cellular crosstalk in salivary gland fibrosis

Journal of Dental Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Triclosan exposure induces liver fibrosis in mice: The heterogeneous nuclear ribonucleoprotein A1/pyruvate kinase M2 axis drives hepatic stellate cell activation

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 294, P. 118113 - 118113

Published: March 30, 2025

Language: Английский

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