Hydrogel-Based Scaffolds: Advancing Bone Regeneration Through Tissue Engineering DOI Creative Commons

Jalima Quintero,

Rosalío Ramos‐Payán, José Geovanni Romero-Quintana

et al.

Gels, Journal Year: 2025, Volume and Issue: 11(3), P. 175 - 175

Published: Feb. 27, 2025

Bone tissue engineering has emerged as a promising approach to addressing the limitations of traditional bone grafts for repairing defects. This regenerative medicine strategy leverages biomaterials, growth factors, and cells create favorable environment regeneration, mimicking body’s natural healing process. Among various biomaterials explored, hydrogels (HGs), class three-dimensional, hydrophilic polymer networks, have gained significant attention scaffolds engineering. Thus, this review aimed investigate potential synthetic HGs, molecules used its functionalization, enhanced applications. HGs offer several advantages such scaffolds, including biocompatibility, biodegradability, tunable mechanical properties, ability encapsulate deliver bioactive molecules. These properties make them ideal candidates supporting cell attachment, proliferation, differentiation, ultimately guiding formation new tissue. The design optimization HG-based involve adapting their composition, structure, meet specific requirements regeneration. Current research focuses on incorporating molecules, factors cytokines, into HG further enhance osteoinductive osteoconductive properties. Additionally, strategies improve strength degradation kinetics are being explored ensure long-term stability support formation. development advanced holds great revolutionizing providing effective treatment options patients with

Language: Английский

Strategies of functionalized GelMA-based bioinks for bone regeneration: Recent advances and future perspectives DOI Creative Commons

Yaru Zhu,

Xingge Yu,

Hao Liu

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 346 - 373

Published: May 9, 2024

Gelatin methacryloyl (GelMA) hydrogels is a widely used bioink because of its good biological properties and tunable physicochemical properties, which has been in variety tissue engineering regeneration. However, pure GelMA limited by the weak mechanical strength lack continuous osteogenic induction environment, difficult to meet needs bone repair. Moreover, are unable respond complex stimuli therefore adapt physiological pathological microenvironments. This review focused on functionalization strategies hydrogel based bioinks for The synthesis process was described details, various functional methods requirements regeneration, including strength, porosity, vascularization, differentiation, immunoregulation patient specific repair, etc. In addition, response smart GelMA-based external physical stimulation internal microenvironment stimulation, as well achieve both disease treatment regeneration presence common diseases (such inflammation, infection, tumor) also briefly reviewed. Finally, we emphasized current challenges possible exploration directions

Language: Английский

Citations

30
Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering DOI Creative Commons
Tomasz Górnicki, Jakub Lambrinow, Afsaneh Golkar‐Narenji

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(6), P. 531 - 531

Published: March 16, 2024

Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic adds complexity to traditional cell cultivation methods. most commonly used technique involves cultivating cells on flat surface in two-dimensional format due its simplicity. A three-dimensional (3D) provide microenvironment for surrounding cells. There two main techniques obtaining 3D structures based the presence scaffolding. Scaffold-free consist spheroid technologies. Meanwhile, scaffold contain organoids all constructs that various types scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels one extensively studied forms potential culture up 4D bioprinted biomaterials. bioprinting is important create scaffolds. versatility this allows many different inks, mainly hydrogels, as well inorganic substances. Increasing amounts data evidence vast usage engineering personalized medicine, with area application being regeneration skin musculoskeletal systems. Recent papers also indicate increasing vivo tests products which further strengthen importance branch emphasize need extensive research safe humansbiomimetic tissues organs. In review article, we recent advancements field preceded by an overview technologies led development complex type culture.

Language: Английский

Citations

19
Fostering tissue engineering and regenerative medicine to treat musculoskeletal disorders in bone and muscle DOI Creative Commons
So‐Yeon Park, Khandoker Asiqur Rahaman,

Yu-Chan Kim

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 40, P. 345 - 365

Published: June 15, 2024

Language: Английский

Citations

16
Engineered extracellular vesicles with sequential cell recruitment and osteogenic functions to effectively promote senescent bone repair DOI Creative Commons

Lei Qi,

Jing Wang,

Jinge Yan

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 12, 2025

Senescent mandibular bone repair poses a formidable challenge without completely satisfactory strategy. Endogenous cell recruitment and osteogenic differentiation are two sequential stages in regeneration, disruptions these processes present significant obstacles to senescent repair. To address issues, engineered extracellular vesicles (EV) with stem functions were developed. This study demonstrated that Apt19s-engineered (Apt19s-EV) recognize recruit marrow mesenchymal cells derived from old rats (O-BMSCs) specifically effectively. MiR-376b-5p, identified by RNA sequencing transfection, was significantly decreased O-BMSCs, it selected construct miR-376b-5p-engineered (376b-EV). 376b-EV could promote osteogenesis alleviate senescence of O-BMSCs targeting Camsap1. combine the advantages Apt19s miR-376b-5p, dual (Apt-376b-EV) comprising both miR-376b-5p modifications constructed. further validate its function, Gelatin methacryloyl (GelMA) hydrogel used as carrier Apt-376b-EV@GelMA delivery system. The vitro results have sequentially. Notably, vivo also showed sequentially endogenous enhance new formation fracture critical-sized defect models. In summary, vesicles, Apt-376b-EV, offer an appealing solution for recruiting promoting microenvironment, which may broaden clinical applications EV provide valuable strategies treating bone-related diseases future work.

Language: Английский

Citations

2
Bone Organoids: Recent Advances and Future Challenges DOI
Ding Zhao, Qimanguli Saiding, Yihan Li

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(5)

Published: Dec. 12, 2023

Bone defects stemming from tumorous growths, traumatic events, and diverse conditions present a profound conundrum in clinical practice research. While bone has the inherent ability to regenerate, substantial anomalies require regeneration techniques. organoids represent new concept this field, involving 3D self-assembly of bone-associated stem cells guided vitro with or without extracellular matrix material, resulting tissue that mimics structural, functional, genetic properties native tissue. Within scientific panorama, ascend an esteemed status, securing significant experimental endorsement. Through synthesis current literature pioneering studies, review offers comprehensive survey organoid paradigm, delves into quintessential architecture ontogeny bone, highlights latest progress fabrication. Further, existing challenges prospective directions for future research are identified, advocating interdisciplinary collaboration fully harness potential burgeoning domain. Conclusively, as technology continues mature, its implications both landscapes poised be profound.

Language: Английский

Citations

26
Application of Gelatin-Based Composites in Bone Tissue Engineering DOI Creative Commons

Enguang Wu,

Lianghui Huang,

Yaochun Shen

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e36258 - e36258

Published: Aug. 1, 2024

Natural bone tissue has the certain function of self-regeneration and repair, but it is difficult to repair large damage. Recently, although autologous grafting "gold standard" for improving high cost, few donor sources. Besides, allogeneic causes greater immune reactions, which hardly meet clinical needs. The engineering (BTE) been developed promote repair. Gelatin, due its biocompatibility, receives a great deal attention in BTE research field. However, disadvantages natural gelatin are poor mechanical properties single structural property. With development BTE, often used combination with range natural, synthetic polymers, inorganic materials achieve synergistic effects complex physiological process review delves into fundamental structure unique gelatin, as well excellent necessary scaffold materials. Then this explores application modified three-dimensional (3D) scaffolds various structures including 3D fiber scaffolds, hydrogels, nanoparticles. In addition, focuses on efficacy composite consisting or polymeric materials, bioactive ceramics metallic/non-metallic defects. these gelatin-based provides new ideas design good biosafety.

Language: Английский

Citations

13
Chitosan-based biomaterials for bone tissue engineering DOI
Youbin Li, Xudong Li, Liwei Zhu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140923 - 140923

Published: Feb. 1, 2025

Language: Английский

Citations

1
Enhanced osteogenic and ROS-scavenging MXene nanosheets incorporated gelatin-based nanocomposite hydrogels for critical-sized calvarial defect repair DOI
J Zhao,

T.C. Wang,

Yuanchao Zhu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 269, P. 131914 - 131914

Published: May 3, 2024

Language: Английский

Citations

7
Exploring the frontiers: the potential and challenges of bioactive scaffolds in osteosarcoma treatment and bone regeneration DOI Creative Commons
Huaiyuan Zhang, Yu Wang,

Huifen Qiang

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101276 - 101276

Published: Sept. 30, 2024

Language: Английский

Citations

7
Localized delivery of metformin via 3D printed GelMA-Nanoclay hydrogel scaffold for enhanced treatment of diabetic bone defects DOI Creative Commons

Hetong Li,

Beini Mao,

Jintao Zhong

et al.

Journal of Orthopaedic Translation, Journal Year: 2024, Volume and Issue: 47, P. 249 - 260

Published: July 1, 2024

Diabetic bone defects present significant challenges for individuals with diabetes. While metformin has been explored regeneration via local delivery, its application in treating diabetic remains under-explored. In this study, we aim to leverage 3D printing technology fabricate a GelMA-Nanoclay hydrogel scaffold loaded specifically purpose. The objective is assess whether the situ release of can effectively enhance osteogenesis, angiogenesis, and immunomodulation context defects. Utilizing technology, constructed GelMA-Nanoclay-Metformin optimal physical properties biocompatibility. osteogenic, angiogenic, immunomodulatory characteristics were thoroughly investigated through both vitro vivo experiments. GelMA10%-Nanoclay8%-Metformin5mg/mL was selected as bioink due favorable swelling rate, degradation mechanical strength, drug rate. Through investigations, extract, enriched metformin, demonstrated substantial enhancement proliferation migration BMSCs within high-glucose microenvironment. It enhances immunomodulation. experimental outcomes further underscored efficacy metformin-loaded promoting superior conclusion, while previous studies have delivery regeneration, our research pioneering using printed scaffold. This localized approach demonstrates potential enhancing patients, offering novel Our study demonstrates, first time, successful loading systemic antidiabetic onto delivery. exhibits mending defects, presenting promising new avenue treatment such conditions.

Language: Английский

Citations

6