Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy

Light Science & Applications, Journal Year: 2022, Volume and Issue: 11(1)

Published: June 1, 2022

We present a new label-free three-dimensional (3D) microscopy technique, termed transport of intensity diffraction tomography with non-interferometric synthetic aperture (TIDT-NSA). Without resorting to interferometric detection, TIDT-NSA retrieves the 3D refractive index (RI) distribution biological specimens from intensity-only measurements at various illumination angles, allowing incoherent-diffraction-limited quantitative phase-contrast imaging. The unique combination z-scanning sample angle diversity in provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution thick samples. Based on an off-the-shelf bright-field microscope programmable light-emitting-diode (LED) source, achieves imaging resolution 206 nm laterally 520 axially high-NA oil immersion objective. validate RI tomographic performance unlabeled fixed live samples, including human breast cancer cell lines MCF-7, hepatocyte carcinoma HepG2, mouse macrophage RAW 264.7, Caenorhabditis elegans (C. elegans), Henrietta Lacks (HeLa) cells. These results establish as approach microscopy, permitting characterization morphology time-dependent subcellular changes widespread medical applications.

Language: Английский

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Dual-plane coupled phase retrieval for non-prior holographic imaging

PhotoniX, Journal Year: 2022, Volume and Issue: 3(1)

Published: Jan. 28, 2022

Abstract Accurate depiction of waves in temporal and spatial is essential to the investigation interactions between physical objects waves. Digital holography (DH) can perform quantitative analysis wave–matter interactions. Full detector-bandwidth reconstruction be realized based on in-line DH. But overlapping twin images strongly prevents analysis. For off-axis DH, object wave detector bandwidth need satisfy certain conditions accurately. Here, we present a reliable approach involving coupled configuration for combining two holograms one hologram, using rapidly converging iterative procedure two-plane phase retrieval (TwPCPR) method. It realizes fast-convergence holographic calculation High-resolution full-field by exploiting full are demonstrated complex-amplitude reconstruction. Off-axis optimization provides an effective initial guess avoid stagnation minimize required measurements multi-plane retrieval. The proposed strategy works well more extended samples without any prior assumptions including support, non-negative, sparse constraints, etc. helps enhance empower applications wavefront sensing, computational microscopy biological tissue

Language: Английский

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Structured illumination microscopy based on principal component analysis

eLight, Journal Year: 2023, Volume and Issue: 3(1)

Published: Feb. 7, 2023

Abstract Structured illumination microscopy (SIM) is one of the powerful super-resolution modalities in bioscience with advantages full-field imaging and high photon efficiency. However, artifact-free image reconstruction requires precise knowledge about parameters. The sample- environment-dependent on-the-fly experimental parameters need to be retrieved a posteriori from acquired data, posing major challenge for real-time, long-term live-cell imaging, where low photobleaching, phototoxicity, light dose are must. In this work, we present an efficient robust SIM algorithm based on principal component analysis (PCA-SIM). PCA-SIM observation that ideal phasor matrix pattern rank one, leading complexity, identification noninteger pixel wave vector phase while rejecting components unrelated parameter estimation. We demonstrate achieves non-iteratively fast, accurate (below 0.01-pixel 0.1 $$\%$$ % 2 $$\pi$$ π relative under typical noise level), estimation at SNRs, which allows real-time live cells complicated scenarios other state-of-the-art methods inevitably fail. particular, provide open-source MATLAB toolbox our associated datasets. combination iteration-free reconstruction, robustness noise, limited computational complexity makes promising method high-speed, long-term, cells.

Language: Английский

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Adaptive optical quantitative phase imaging based on annular illumination Fourier ptychographic microscopy

PhotoniX, Journal Year: 2022, Volume and Issue: 3(1)

Published: Oct. 21, 2022

Abstract Quantitative phase imaging (QPI) has emerged as a valuable tool for biomedical research thanks to its unique capabilities quantifying optical thickness variation of living cells and tissues. Among many QPI methods, Fourier ptychographic microscopy (FPM) allows long-term label-free observation quantitative analysis large cell populations without compromising spatial temporal resolution. However, high spatio-temporal resolution over long-time scale (from hours days) remains critical challenge: optically inhomogeneous structure biological specimens well mechanical perturbations thermal fluctuations the microscope body all result in time-varying aberration focus drifts, significantly degrading performance study. Moreover, aberrations are sample- environment-dependent, cannot be compensated by fixed design, thus necessitating rapid dynamic correction process. Here, we report an adaptive method based on annular illumination FPM. In this method, matched configuration (i.e., numerical aperture (NA) strictly equals objective NA), which is key recovering low-frequency information, further utilized accurate characterization. By using only 6 low-resolution images captured with different angles matching NA 10x, 0.4 objective, recover high-resolution (synthetic 0.8) characterize real time, restoring optimum system adaptively. Applying our live-cell imaging, achieve diffraction-limited (full-pitch $$655\,nm$$ 655 n m at wavelength $$525\,nm$$ 525 ) across wide field view ( $$1.77\,mm^2$$ 1.77 2 extended period time.

Language: Английский

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Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Sept. 5, 2024

Language: Английский

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Continuous optical zoom microscope with extended depth of field and 3D reconstruction

PhotoniX, Journal Year: 2022, Volume and Issue: 3(1)

Published: Sept. 15, 2022

Abstract Microscope such as fluorescence microscope, confocal microscope and two-photon plays an important role in life science, laser processing other fields. However, most microscopes only have discrete zoom rates. In this paper, a continuous optical with extended depth of field 3D reconstruction is demonstrated for the first time. It consists objective lens, holder, adjustable three-dimensional object stage, Abbe condenser LED light source. The lens composed several liquid lenses solid lenses. By adjusting applied voltage to proposed can achieve large magnification from 10? 60?. Moreover, improved shape focus (SFF) algorithm image fusion are designed reproduction. Based on lenses, axial focusing position be adjusted obtain images different depths, then realized. Our experimental results demonstrate feasibility microscope. expected fields pathological diagnosis, biological detection, etc.

Language: Английский

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Transport-of-intensity Fourier ptychographic diffraction tomography: defying the matched illumination condition

Optica, Journal Year: 2022, Volume and Issue: 9(12), P. 1362 - 1362

Published: Oct. 26, 2022

Optical diffraction tomography (ODT) is a promising label-free three-dimensional (3D) microscopic method capable of measuring the 3D refractive index (RI) distribution optically transparent samples (e.g., unlabeled biological cells). In recent years, non-interferometric ODT techniques have received increasing attention for their system simplicity, speckle-free imaging quality, and compatibility with existing microscopes. However, methods implementing measurements in high numerical aperture (NA) microscopy systems are often plagued by low-frequency missing problems—a consequence violating matched illumination condition. Here, we present transport-of-intensity Fourier ptychographic (TI-FPDT) to address this challenging issue combining angular diversity additional “transport intensity” measurements. TI-FPDT exploits defocused phase contrast circumvent stringent requirement on NA imposed It effectively overcomes reconstruction quality deterioration RI underestimation problems conventional FPDT, as demonstrated high-resolution tomographic various (including microspheres, USAF targets, HeLa cells, C2C12 Due its simplicity effectiveness, anticipated open new possibilities biomedical applications.

Language: Английский

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Hybrid brightfield and darkfield transport of intensity approach for high-throughput quantitative phase microscopy

Advanced Photonics, Journal Year: 2022, Volume and Issue: 4(05)

Published: Sept. 27, 2022

Transport of intensity equation (TIE) is a well-established non-interferometric phase retrieval approach that enables quantitative imaging (QPI) by simply measuring images at multiple axially displaced planes. The advantage TIE-based QPI system its compatibility with partially coherent illumination, which provides speckle-free resolution beyond the diffraction limit. However, TIE generally implemented brightfield (BF) configuration, and maximum achievable still limited to incoherent limit (twice limit). It desirable TIE-related approaches can surpass this achieve high-throughput [high-resolution wide field view (FOV)] QPI. We propose hybrid BF darkfield transport (HBDTI) for microscopy. Two through-focus stacks corresponding illuminations are acquired through low-numerical-aperture (NA) objective lens. high-resolution large-FOV complex amplitude (both absorption distributions) then be synthesized based on an iterative algorithm taking coherence model decomposition into account. effectiveness proposed method experimentally verified USAF target different types biological cells. experimental results demonstrate half-width improved from 1230 nm 488 2.5 × expansion across 4 FOV 7.19 mm2, 6.25 increase in space-bandwidth product ∼5 ∼30.2 megapixels. In contrast conventional methods where only illumination used, synthetic aperture process HBDTI further incorporates expand accessible object frequency, thereby significantly extending available 2NA ∼5NA promotion Given capability QPI, expected adopted biomedical fields, such as personalized genomics cancer diagnostics.

Language: Английский

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Self‐Powered and Flexible Photodetector with High Polarization Sensitivity Based on MAPbBr₃–MAPbI₃ Microwire Lateral Heterojunction

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(45)

Published: Sept. 2, 2022

Abstract Assembling perovskites into heterojunctions is an effective approach to achieve high‐performance photodetectors. Compared with vertical heterojunctions, in lateral heterojunction‐based photodetectors, the reflection loss reduced because active layer direct contact light resulting higher performance and better stability. However, perovskite–perovskite are difficult using solution methods first formed film easily dissolved by solvent of second precursor. In this study, a two‐step imprinting method developed fabricate MAPbI 3 –MAPbBr microwire realize photodetector responsivity detectivity 1207 A W −1 2.78 × 10 13 Jones, respectively. At 0 V bias, device exhibits up 233 , which more than double previously reported best results. The high‐quality heterojunction endows photodetectors ultra‐high polarization sensitivity ( I max / min = 8.2) long‐term stability, retaining 88.2% its initial even after being exposed air for 391 d.

Language: Английский

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Curved Photodetectors Based on Perovskite Microwire Arrays via In Situ Conformal Nanoimprinting

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(29)

Published: April 28, 2022

Abstract Micro/nano optoelectronic devices based on curved substrates play a significant role in the development of wearable devices, electronic skin, conformal sensors, antennas, and soft robots. However, current fabrication processes are oriented toward planar micro/nano such is challenging. Herein, temperature‐gradient‐assisted nanoimprint‐based situ micro/nano‐crystal growth method proposed to fabricate high‐quality perovskite microwire crystal (MWC) arrays surfaces. Based this MWC array without bending‐induced defects, high‐performance photodetectors (responsivity = 414 A W −1 , detectivity 1.2 × 10 14 Jones, external quantum efficiency over 140 000%) with extraordinary stability (85% original performance maintained for more than 2 years) fabricated realize imaging device. These results provide insights into application nonconformal optical systems.

Language: Английский

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