Quorum sensing interference by phenolic compounds – A matter of bacterial misunderstanding

Heliyon, Journal Year: 2023, Volume and Issue: 9(7), P. e17657 - e17657

Published: June 26, 2023

Over the past decade, numerous publications have emerged in literature focusing on inhibition of quorum sensing (QS) by plant extracts and phenolic compounds. However, there is still a scarcity studies that delve into specific mechanisms which these compounds inhibit QS. Thus, our question whether can QS or indirect manner to elucidate underlying involved. This study focused most studied system, namely, auto-inducer (AI-1) type QS, represented N-acyl-homoserine lactone (AHL) signals AHL-mediated responses. Here, we analyzed recent order understand how act at cellular level, sub-inhibitory concentrations, evaluated they may act. The biotechnological application inhibitors holds promising prospects for pharmaceutical food industries, serving as adjunct therapies prevention biofilms various surfaces.

Language: Английский

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Interaction of Jania rubens Polyphenolic Extract as an Antidiabetic Agent with α-Amylase, Lipase, and Trypsin: In Vitro Evaluations and In Silico Studies

Catalysts, Journal Year: 2023, Volume and Issue: 13(2), P. 443 - 443

Published: Feb. 18, 2023

Jania rubens red seaweed has various bioactive compounds that can be used for several medicinal and pharmaceutical applications. In this study, we investigate the antidiabetic, anti-inflammatory, antioxidant competency of polyphenolic extract (JRPE) by assessing their interactions with α-amylase, lipase, trypsin enzymes. HPLC analysis revealed dominance twelve compounds. We performed computational using as target proteins polyphenols to explore activities based on predicted modes binding sites following molecular modeling analysis. The docking demonstrated a good affinity score noticeable compositions rubens. highest α-amylase (PDB: 4W93) were kaempferol, quercetin, chlorogenic acid, −8.4, −8.8 −8 kcal/mol, respectively. Similarly, lipase 1LPB) high scores −7.1, −7.4, −7.2 kcal/mol Furthermore, 4DOQ) results, acid −7.2, −7.1 findings verified in vitro evaluations, manifesting comparable results. Overall, these enlighten JRPE properties different diabetics’ enzymes could further studied vivo investigations diabetes treatment.

Language: Английский

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Synergistic Effect of Plant Compounds in Combination with Conventional Antimicrobials against Biofilm of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida spp.

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(11), P. 1531 - 1531

Published: Oct. 30, 2023

Bacterial and fungal biofilm has increased antibiotic resistance plays an essential role in many persistent diseases. Biofilm-associated chronic infections are difficult to treat reduce the efficacy of medical devices. This global problem prompted extensive research find alternative strategies fight microbial infections. Plant bioactive metabolites with antibiofilm activity known be potential resources alleviate this problem. The phytochemical screening some medicinal plants showed different active groups, such as stilbenes, tannins, alkaloids, terpenes, polyphenolics, flavonoids, lignans, quinones, coumarins. Synergistic effects can observed interaction between plant compounds conventional drugs. review analyses summarises current knowledge on synergistic combination antimicrobials against biofilms Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans. synergism modify inhibit mechanisms acquired resistance, undesirable effects, obtain appropriate therapeutic effect at lower doses. A deeper these combinations their possible targets is needed develop next-generation novel and/or improve drug-resistant attributed biofilm.

Language: Английский

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Evaluation of broad-spectrum antibacterial efficacy of quercetin by molecular docking, molecular dynamics simulation and in vitro studies

Chemical Physics Impact, Journal Year: 2024, Volume and Issue: 8, P. 100501 - 100501

Published: Feb. 2, 2024

This study aims to determine the antibacterial efficacy of a bioactive phytochemical quercetin (QUR), compared reference antibiotic ciprofloxacin (CIP), using in vitro and silico approaches. Following molecular docking, QUR showed strong affinity druggable targets from bacteria: Sortase B, Toxic Shock Syndrome Toxin-1, Multidrug Efflux Pump AdeJ LasR, with binding energies -6.9 – -10.3 kcal/mol. CIP displayed -7.3 -7.4 kcal/mol against target proteins. had stronger MEPJ (binding energy: -8.1 kcal/mol) LasR kcal/mol), having kcal/mol, respectively. Molecular dynamics simulation validated formation complex QUR, which was energetically more stable LasR-CIP complex. Disc diffusion method, nutrient agar medium, demonstrated activity both gram-negative (Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa) gram-positive (Staphylococcus aureus, Streptococcus pneumonia) bacteria zone diameter inhibition 10 23 mm 7 21 mm, synergistic effect, growth inhibitory index (GII) values 0.65 1.15, combined PIP. QUR-CEF combination synergy E. P. aeruginosa, S. pneumoniae GIIs 0.60 0.73. Pharmacokinetics studies through for revealed acceptable parametric values, comparable that CIP. Thus, current integrated analysis authenticated as lead molecule combat bacterial infections, alone or conventionally used antibiotics (PIP CEF). For further validation our results, clinical experiments are suggested.

Language: Английский

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Antibacterial activity of hydrophobicity modulated cationic polymers with enzyme and pH-responsiveness

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(11), P. 2894 - 2904

Published: Jan. 1, 2024

Lysine and fatty acid-tethered amphiphilic copolymers exhibited potent antibacterial activity against Gram-positive ( B. subtilis ) Gram-negative E. coli bacteria with side chain degradability in the presence of an enzyme pH.

Language: Английский

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Exploring Phenolic Compounds as Quorum Sensing Inhibitors in Foodborne Bacteria

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: Sept. 14, 2021

The emergence of multidrug-resistant bacteria stimulates the search for new substitutes to traditional antimicrobial agents, especially molecules with antivirulence properties, such as those that interfere quorum sensing (QS). This study aimed evaluate potential phenolic compounds QS inhibition in a biosensor strain ( Chromobacterium violaceum ) and three foodborne bacterial species Aeromonas hydrophila , Salmonella enterica serovar Montevideo, Serratia marcescens ). Initially, an silico molecular docking was performed select greatest inhibition, using structural variants CviR regulator C. target. Curcumin, capsaicin, resveratrol, gallic acid, phloridizin presented good affinity at least four variants. These were tested activity, biofilm formation, anti-QS activity. activity when combined kanamycin also assessed. resveratrol inhibited up 50% violacein production by . Biofilm formation 80% A. capsaicin curcumin 40% S. Montevideo 60% Curcumin completely swarming motility Additionally, increased sensitivity kanamycin. results indicate concentrations low 6μM are inhibitors besides having properties higher concentrations, encouraging applications food pharmaceutical industries.

Language: Английский

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The chemical ecology of the fungus-farming termite symbiosis

Natural Product Reports, Journal Year: 2021, Volume and Issue: 39(2), P. 231 - 248

Published: Aug. 19, 2021

Since the early 1970s, 375 natural products have been identified from members of fungus-farming termite symbiosis, and this review summarises discusses ecological implications presence vast chemical repertoire.

Language: Английский

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Potential of Flavonoids as Promising Phytotherapeutic Agents to Combat Multidrug-Resistant Infections

Current Pharmaceutical Biotechnology, Journal Year: 2024, Volume and Issue: 25(13), P. 1664 - 1692

Published: Oct. 1, 2024

Considering the limited number of current effective treatments, Multidrug- Resistant (MDR) illnesses have grown to be a serious concern public health. It has become necessary look for new antimicrobial drugs because emergence resistance numerous kinds antibiotics. The use flavonoids is one phytotherapeutic strategy that been researched as potential remedy this issue. Secondary plant compounds called found an antibacterial effect against resistant microorganisms.

Language: Английский

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Mesoporous Silica Materials Loaded with Gallic Acid with Antimicrobial Potential

Nanomaterials, Journal Year: 2022, Volume and Issue: 12(10), P. 1648 - 1648

Published: May 12, 2022

This paper aimed to develop two types of support materials with a mesoporous structure mobile crystalline matter (known in the literature as MCM, namely MCM-41 and MCM-48) load them gallic acid. Soft templating methodology was chosen for preparation structures-the cylindrical micelles certain structural characteristics being formed due hydrophilic hydrophobic intermolecular forces which occur between molecules surfactants (cetyltrimethylammonium bromide-CTAB) when minimal micellar ionic concentration is reached. These supports were loaded acid using three different MCM-gallic ratios (1:0.41; 1:0.82 1:1.21)-and their characterizations by FTIR, SEM, XRD, BET drug release performed. It worth mentioning that loading carried out vacuum-assisted methodology: are firstly kept under vacuum at ~0.1 barr 30 min followed addition polyphenol solutions. The solutions adapted such final volume covered wet and-in this case-upon reaching normal atmospheric pressure, solution pushed inside pores, thus polyphenols mainly pores. Based on SBET data, it can be seen specific surface area decreased considerably increasing ratio acid; 3.07 4.25 times MCM-48, respectively. sample highest content further evaluated from biological point view, alone or association amoxicillin administration. As expected, MCM-48 not protective against infections-but, acid, potentiated inhibition recorded tested gram-negative bacterial strains. Moreover, important mention these systems efficient recovery gut microbiota after exposure antibiotics, instance.

Language: Английский

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N-acetylcysteine (NAC) attenuates quorum sensing regulated phenotypes in Pseudomonas aeruginosa PAO1

Heliyon, Journal Year: 2023, Volume and Issue: 9(3), P. e14152 - e14152

Published: Feb. 28, 2023

The expression of many virulence genes in bacteria is regulated by quorum sensing (QS), and the inhibition this mechanism has been intensely investigated. N-acetylcysteine (NAC) good antibacterial activity able to interfere with biofilm-related respiratory infections, but little known whether compound an effect on bacterial QS communication. This work aimed evaluate potential NAC as a inhibitor (QSI) Pseudomonas aeruginosa PAO1 through silico vitro analyses, well combination antibiotic tobramycin. Initially, molecular docking analysis was performed between regulatory proteins, LasR RhlR, P. NAC, 3-oxo-C12-HSL, C4-HSL, furanone C30. sub-inhibitory concentration determined growth curves. Then, we tests using reporter strains lasB-gfp rhlA-gfp, QS-related phenotypes. Finally, synergistic tobramycin calculated fractional inhibitory concentrations index (FICi) investigated against growth, pigment production, biofilm formation. In study, bound RhlR proteins similar manner AHL cognate, suggesting that it may be bind receptor vivo. biosensor assay, GFP signal turned down presence at 1000, 500, 250, 125 μM for rhlA-gfp (p < 0.05), effect. Pyocyanin rhamnolipids decreased 0.05) up 34 37%, respectively, μM. Swarming swimming motilities were inhibited 250 10000 Additionally, 2500 reduced NAC-tobramycin showed FICi 0.8, best 2500–1.07 μM, inhibiting formation 60%, besides reducing pyocyanin pyoverdine production. Confocal microscopy images revealed stronger, dense, compact control, while treated became thinner more dispersed. Overall, low promising anti-QS properties PAO1, adding its already antibiofilm agent.

Language: Английский

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