Exosomes Derived From M2 Macrophages Facilitate Osteogenesis and Reduce Adipogenesis of BMSCs

Frontiers in Endocrinology, Journal Year: 2021, Volume and Issue: 12

Published: July 6, 2021

Bone regeneration is a complex process that requires the coordination of osteogenesis and osteoclastogenesis. The balance between adipogenesis bone marrow mesenchymal stem cells (BMSCs) plays major role in formation. Recently, intercellular communication surrounding has been gradually recognized, macrophages on surface have proven to regulate metabolism. However, underlying mechanisms not fully elucidated. Recent studies indicated exosomes are vital messengers for cell-cell various biological processes. In this experiment, we found derived from M2 (M2D-Exos) could inhibit promote BMSCs. M2D-Exo intervention increased expression miR-690, IRS-1, TAZ Additionally, miR-690 knockdown with inhibitor partially counteracted effect M2D-Exos BMSC differentiation upregulation IRS-1 expression. Taken together, results our study indicate isolated facilitate reduce through miR-690/IRS-1/TAZ axis might be therapeutic tool loss diseases.

Language: Английский

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Pathogenesis of Aging and Age-related Comorbidities in People with HIV: Highlights from the HIV ACTION Workshop

Pathogens and Immunity, Journal Year: 2020, Volume and Issue: 5(1), P. 143 - 143

Published: June 17, 2020

People with HIV (PWH) experience accentuated biological aging, as defined by markers of inflammation, immune dysfunction, and the epigenetic clock. They also have an elevated risk multiple age-associated comorbidities. To discuss current knowledge, research gaps, priorities in aging age-related comorbidities treated infection, NIH program staff organized a workshop held Bethesda, Maryland September 2019. This review article describes highlights discussions led Pathogenesis/Basic Science Research working group that focused on three high priority topics: immunopathogenesis; microbiome/virome; senescence. We summarize knowledge these fields describe key questions for pathogenesis PWH. Understanding drivers mechanisms underlying is will help identify potential therapeutic targets to improve healthspan older

Language: Английский

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Lignans as Pharmacological Agents in Disorders Related to Oxidative Stress and Inflammation: Chemical Synthesis Approaches and Biological Activities

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(11), P. 6031 - 6031

Published: May 27, 2022

Plant lignans exhibit a wide range of biological activities, which makes them the research objects potential use as therapeutic agents. They provide diverse naturally-occurring pharmacophores and are available for production by chemical synthesis. A large amount accumulated data indicates that different structural groups apt to demonstrate both anti-inflammatory antioxidant effects, in many cases, simultaneously. In this review, we summarize comprehensive knowledge about lignan bioactive agent disorders associated with oxidative stress inflammation, pharmacological effects vitro vivo, molecular mechanisms underlying these synthesis approaches. This article provides an up-to-date overview current area, PubMed, Scopus, Web Science databases, screened from 2000 2022.

Language: Английский

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Harnessing the power of Arctium lappa root: a review of its pharmacological properties and therapeutic applications

Natural Products and Bioprospecting, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 20, 2024

Arctium lappa, widely recognized as burdock, is a perennial plant that employed in the realm of traditional Chinese medicine for wide range medicinal applications. The herb rich bioactive metabolites with therapeutic potential, encompassing polyphenolic antioxidants its leaves, and flavonoids fructo-oligosaccharides underground parts. Nutraceuticals originating from botanical sources such lappa provide supplementary health advantages alongside their nutritional content have demonstrated effectiveness prevention management specific ailments. utilization root extract has exhibited encouraging outcomes addressing hepatotoxicity induced by cadmium, lead, chromium, acetaminophen, ameliorating liver damage oxidative stress. Additionally, displays properties antidiabetic, hypolipidemic, aphrodisiac, anti-rheumatic, anti-Alzheimer, various other pharmacological actions.

Language: Английский

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The neuroprotective potential of phytochemicals in traumatic brain injury: mechanistic insights and pharmacological implications

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 4, 2024

Traumatic brain injury (TBI) leads to damage, comprising both immediate primary damage and a subsequent cascade of secondary mechanisms. The results in localized while the initiates inflammatory responses, followed by disruption blood-brain barrier, infiltration peripheral blood cells, edema, release various immune mediators, including chemotactic factors interleukins. TBI disrupts molecular signaling, cell structures, functions. In addition physical tissue such as axonal injuries, contusions, haemorrhages, interferes with functioning, impacting cognition, decision-making, memory, attention, speech capabilities. Despite deep understanding pathophysiology TBI, an intensive effort evaluate underlying mechanisms effective therapeutic interventions is imperative manage repercussions TBI. Studies have commenced explore potential employing natural compounds for These are characterized their low toxicity limited interactions conventional drugs. Moreover, many demonstrate capacity target aspects process. While our there urgent need mitigate its consequences. Here, we aimed summarize mechanism action role phytochemicals against progression. This review discusses implications phytonutrients addresses consequences addition, highlighted roles emerging promising candidates intervention highlights neuroprotective mechanistic approach. Furthermore, efforts focused on mechanisms, providing better therapeutics.

Language: Английский

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Matairesinol Induces Mitochondrial Dysfunction and Exerts Synergistic Anticancer Effects with 5-Fluorouracil in Pancreatic Cancer Cells

Marine Drugs, Journal Year: 2022, Volume and Issue: 20(8), P. 473 - 473

Published: July 25, 2022

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive types cancer and exhibits a devastating 5-year survival rate. The recent procedure for treatment PDAC combination several conventional chemotherapeutic agents, termed FOLFIRINOX, that includes irinotecan, leucovorin, oxaliplatin, 5-fluorouracil (5-FU). However, ongoing using these agents challenging due to their severe side effects limitations on range patients available PDAC. Therefore, safer more innovative anticancer must be developed. anticarcinoma activity matairesinol can extracted from seagrass has been reported in various cancer, including prostate, breast, cervical, pancreatic cancer. molecular mechanism effective against remains unclear. In present study, we confirmed inhibition cell proliferation progression induced by representative human lines (MIA PaCa-2 PANC-1). Additionally, triggers apoptosis causes mitochondrial impairment as evidenced depolarization membrane, disruption calcium, suppression migration related intracellular signaling pathways. Finally, exerts synergistic effect with 5-FU, standard agent These results demonstrate therapeutic potential

Language: Английский

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Biochemical and Molecular Insights into Variation in Sesame Seed Antioxidant Capability as Revealed by Metabolomics and Transcriptomics Analysis

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 514 - 514

Published: April 25, 2024

Sesame seeds are important resources for relieving oxidation stress-related diseases. Although a significant variation in seeds’ antioxidant capability is observed, the underlying biochemical and molecular basis remains elusive. Thus, this study aimed to reveal major seed components key mechanisms that drive variability of activity (AOA) using panel 400 sesame accessions. The AOA, total flavonoid, phenolic contents varied from 2.03 78.5%, 0.072 3.104 mg CAE/g, 2.717 21.98 GAE/g, respectively. Analyses revealed flavonoids acids main contributors AOA variation, irrespective coat color. LC-MS-based polyphenol profiling high (HA) low (LA) uncovered 320 differentially accumulated compounds (DAPs), including 311 up-regulated HA seeds. Tricin, persicoside, 5,7,4′,5′-tetrahydro-3′,6-dimethoxyflavone, 8-methoxyapigenin, 6,7,8-tetrahydroxy-5-methoxyflavone were top five HA. Comparative transcriptome analysis at three developmental stages identified 627~2357 DEGs unveiled differential regulation flavonoid biosynthesis, phenylpropanoid stilbene biosynthesis capacity variation. Major regulated structural genes transcription factors identified. SINPZ0000571 (MYB), SINPZ0401118 (NAC), SINPZ0500871 (C3H) most highly induced TFs Our findings may enhance quality breeding.

Language: Английский

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Resveratrol Alleviates Arsenic Exposure-Induced Liver Fibrosis in Rats by Inhibiting Hepatocyte Senescence

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: June 3, 2024

Language: Английский

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Targeting NF-κB pathway by dietary lignans in inflammation: expanding roles of gut microbiota and metabolites

Critical Reviews in Food Science and Nutrition, Journal Year: 2022, Volume and Issue: 63(22), P. 5967 - 5983

Published: Jan. 24, 2022

Inflammation is a major factor affecting human health. Nuclear factor-kappa B (NF-κB) plays vital role in the development of inflammation, and promoters most inflammatory cytokine genes have NF-κB-binding sites. Targeting NF-κB could be an exciting route for prevention treatment diseases. As important constituents natural plants, lignans are proved to numerous biological functions. There growing pieces evidence demonstrate that potential anti-inflammatory activities. In this work, type, structure source influence on mitigating inflammation systematically summarized. This review focuses targeting signaling pathway response by different their molecular mechanisms. Lignans also regulate gut microflora change microbial metabolites, which exert novel prevent activation. Taken together, target with various mechanisms inhibit expressions response. It will provide scientific theoretical basis further research effects functional foods.

Language: Английский

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Correlation between METTL3 overexpression and 18F-FDG uptake in patients with soft tissue sarcoma

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 7, 2025

N6-methyladenosine (m6A) methylation plays a key role in tumor progression. However, the significance of methyltransferase-like 3 (METTL3) biological processes soft tissue sarcoma (STS) patients, and relationship between METTL3 STS are unclear. The expression its with patient prognosis were determined from database analyses. Immunohistochemical staining 18F-FDG radioautography performed on tissues 39 patients undergoing PET before treatment. peritumoral was evaluated Wilcoxon test. Mann-Whitney U test Spearman's correlation analysis used to explore correlations both clinicopathological characteristics uptake. One-way variance ROC evaluate efficacy metabolic parameters predicting expression. significantly higher than normal (all p values<0.01), correlated poor (p < 0.05). associated histological differentiation (Z=-2.026, = 0.043), but no significant difference observed according age, sex, size, location, or metastasis values > positively CD163 (r 0.502, 0.011), CD68 0.381, 0.017), CD8 0.319, 0.048), exhibited trend toward CD4 0.310, 0.055). Moreover, metabolism 0.580 for r 0.434 radioautography, all values<0.01). SUVmax tumors high rather low (Z=-2.979, 0.003). overexpressed STS, which may be meaningful target action patients. uptake elevated expression, could provide imaging biomarker

Language: Английский

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