Biomimetic AgNPs@antimicrobial peptide/silk fibroin coating for infection-trigger antibacterial capability and enhanced osseointegration

Bioactive Materials, Journal Year: 2022, Volume and Issue: 20, P. 64 - 80

Published: May 20, 2022

Endowing implant surfaces with combined antibacterial and osteogenic properties by drug-loaded coatings has made great strides, but how to achieve the excellence of infection-triggered bactericidal in vivo-proven activities without causing bacterial resistance still remains a formidable challenge. Herein, antimicrobial peptides (AMPs) fragments were designed complexed on surface silver nanoparticle (AgNP) through hydrogen bonding, collagen structure-bionic silk fibroin (SF) was applied carry AgNPs@ AMPs osteointegration. As verified TEM, contributed dispersion size-regulation AgNPs, particle size about 20 nm, clear protein corona structure observed surface. The release curve ion displayed that SF-based coating owned sensitive pH-responsive properties. In test against S.aureus for up 21 days, rate had always remained above 99%. Meanwhile, underlying mechanism revealed, originating from destruction cell membranes ROS generation. conducive adhesion, diffusion, proliferation bone marrow stem cells (BMSCs) surface, promoted expression genes secretion. vivo implantation results showed compared untreated Ti implants, enhanced osseointegration at week 4 8. Overall, AgNPs@AMPs-loaded presented ability synergistically inhibit bacteria promote osseointegration, possessing tremendous potential application prospects defects related-infection treatments.

Language: Английский

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Advances in silver nanoparticles: a comprehensive review on their potential as antimicrobial agents and their mechanisms of action elucidated by proteomics

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 31, 2024

Nanoparticles play a crucial role in the field of nanotechnology, offering different properties due to their surface area attributed small size. Among them, silver nanoparticles (AgNPs) have attracted significant attention antimicrobial properties, with applications that date back from ancient medicinal practices contemporary commercial products containing ions or nanoparticles. AgNPs possess broad-spectrum biocidal potential against bacteria, fungi, viruses, and Mycobacterium, addition exhibiting synergistic effects when combined certain antibiotics. The mechanisms underlying its action include generation oxygen-reactive species, damage DNA, rupture bacterial cell membranes inhibition protein synthesis. Recent studies highlighted effectiveness various clinically relevant strains through combat antibiotic-resistant pathogens. This review investigates proteomic by which exert effects, special focus on activity planktonic bacteria biofilms. Furthermore, it discusses biomedical non-preparation antibiotic formulations, also addressing issue resistance

Language: Английский

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Multifunctional Ac@ZIF-8/AgNPs nanoplatform with pH-responsive and ROS scavenging antibacterial properties promotes infected wound healing

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 489, P. 151485 - 151485

Published: April 20, 2024

Language: Английский

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Eco-biofabrication of silver nanoparticles from Azadirachta indica, Gymnema sylvestre, and Moringa oleifera for lung cancer treatment

Journal of the Egyptian National Cancer Institute, Journal Year: 2025, Volume and Issue: 37(1)

Published: Jan. 6, 2025

Silver nanoparticles (AgNPs) derived from natural sources have garnered significant attention due to their unique properties and eco-friendly production methods. With lung cancer remaining a major global health issue, there is continuous need for novel effective therapeutic approaches beyond conventional treatments such as chemotherapy, immunotherapy, targeted therapies.

Language: Английский

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Bio-inspired natural platelet hydrogels for wound healing

Science Bulletin, Journal Year: 2022, Volume and Issue: 67(17), P. 1776 - 1784

Published: July 30, 2022

Language: Английский

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Overcoming Methicillin-Resistance Staphylococcus aureus (MRSA) Using Antimicrobial Peptides-Silver Nanoparticles

Antibiotics, Journal Year: 2022, Volume and Issue: 11(7), P. 951 - 951

Published: July 15, 2022

Antibiotics are regarded as a miracle in the medical field it prevents disease caused by pathogenic bacteria. Since discovery of penicillin, antibiotics have become foundation for modern discoveries. However, bacteria soon became resistant to antibiotics, which puts burden on healthcare system. Methicillin-resistant

Language: Английский

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Silver Nanoparticle-Based Therapy: Can It Be Useful to Combat Multi-Drug Resistant Bacteria?

Antibiotics, Journal Year: 2022, Volume and Issue: 11(9), P. 1205 - 1205

Published: Sept. 6, 2022

The present review focuses on the potential use of silver nanoparticles in therapy diseases caused by antibiotic-resistant bacteria. Such bacteria are known as “superbugs”, and most concerning species Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus (methicillin vancomycin-resistant), some Enterobacteriaceae. According to World Health Organization (WHO), there is an urgent need for new treatments against these “superbugs”. One possible approaches treatment antibacterial nanoparticles. After a short overview nanoparticle usage, mechanisms action, methods synthesis nanoparticles, emphasis has been placed (AgNPs) combat relevant emerging resistant toxicological aspects AgNPs, both vitro using cell cultures vivo have reviewed. It was found that toxic activity AgNPs dependent dose, size, shape, electrical charge. mechanism action involves interactions at various levels such plasma membrane, DNA replication, inactivation protein/enzymes necessary, formation reactive oxygen (ROS) leading death. Researchers do not always agree their conclusions topic more work needed this field before can be effectively applied clinical multi-drug

Language: Английский

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Metal-organic framework-modulated Fe3O4 composite au nanoparticles for antibacterial wound healing via synergistic peroxidase-like nanozymatic catalysis

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 15, 2023

Abstract Bacterial wound infections are a serious threat due to the emergence of antibiotic resistance. Herein, we report an innovative hybrid nanozyme independent antibiotics for antimicrobial healing. The nanozymes fabricated from ultra-small Au NPs via in-situ growth on metal-organic framework (MOF)-stabilised Fe 3 O 4 (Fe @MOF@Au NPs, FMA NPs). displayed synergistic peroxidase (POD)-like activities. It showed remarkable level hydroxyl radicals (·OH) in presence low dose H 2 (0.97 mM). Further, exhibited excellent biocompatibility and favourable antibacterial effects against both Gram-negative (Escherichia coli) Gram-positive ( Staphylococcus aureus ) bacteria. animal experiments indicated that promoted repair with adequate biosafety. Thus, well-designed represent potential strategy healing bacterial infections, without any toxic side effects, suggesting possible applications therapy.

Language: Английский

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Targeting bacterial biofilm-related genes with nanoparticle-based strategies

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 22, 2024

Persistent infection caused by biofilm is an urgent in medicine that should be tackled new alternative strategies. Low efficiency of classical treatments and antibiotic resistance are the main concerns persistent due to formation which increases risk morbidity mortality. The gene expression patterns cells differed from those planktonic cells. One promising approaches against biofilms nanoparticle (NP)-based therapy NPs with multiple mechanisms hinder bacterial or forms. For instance, such as silver (Ag), zinc oxide (ZnO), titanium dioxide (TiO 2 ), copper (Cu), iron (Fe 3 O 4 ) through different strategies interfere bacteria associated biofilm. can penetrate into structure affect efflux pump, quorum-sensing, adhesion-related genes, lead inhibit development. Therefore, understanding targeting genes molecular basis point therapeutic targets make possible control infections. In parallel, impact on environment their cytotoxicity avoided controlled exposure safety assessments. This study focuses biofilm-related potential for inhibition highly effective NPs, especially metal NPs.

Language: Английский

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A Comprehensive Review of Recent Research into the Effects of Antimicrobial Peptides on Biofilms—January 2020 to September 2023

Antibiotics, Journal Year: 2024, Volume and Issue: 13(4), P. 343 - 343

Published: April 9, 2024

Microbial biofilm formation creates a persistent and resistant environment in which microorganisms can survive, contributing to antibiotic resistance chronic inflammatory diseases. Increasingly, biofilms are caused by multi-drug microorganisms, which, coupled with diminishing supply of effective antibiotics, is driving the search for new therapies. In this respect, antimicrobial peptides (AMPs) short, hydrophobic, amphipathic that show activity against multidrug-resistant bacteria formation. They also possess broad-spectrum diverse mechanisms action. comprehensive review, 150 publications (from January 2020 September 2023) were collected categorized using terms ‘polypeptide agent’, ‘antimicrobial peptide’, ‘biofilm’. During period, wide range natural synthetic AMPs studied, LL-37, polymyxin B, GH12, Nisin most frequently cited. Furthermore, although many microbes Staphylococcus aureus Pseudomonas aeruginosa popular. Publications considered AMP combinations potential role delivery systems increasing efficacy AMPs, including nanoparticle delivery. Relatively few focused on resistance. This review informs guides researchers about latest developments research, presenting promising evidence as agents.

Language: Английский

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