3D printed hetero-layered composite scaffold with engineered superficial zone promotes osteogenic differentiation of pre-osteoblast MC3T3-E1 cells

Surfaces and Interfaces, Journal Year: 2024, Volume and Issue: 51, P. 104683 - 104683

Published: June 26, 2024

Language: Английский

---

MiR-21-5p regulates the dynamic of mitochondria network and rejuvenates the senile phenotype of bone marrow stromal cells (BMSCs) isolated from osteoporotic SAM/P6 mice

Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 29, 2023

Abstract Background Progression of senile osteoporosis is associated with deteriorated regenerative potential bone marrow-derived mesenchymal stem/stromal cells (BMSCs). According to the recent results, senescent phenotype osteoporotic strongly correlates impaired regulation mitochondria dynamics. Moreover, due ageing population and growing incidence, more efficient methods concerning BMSCs rejuvenation are intensely investigated. Recently, miR-21-5p was reported play a vital role in turnover, but its therapeutic mechanisms progenitor delivered from patients remain unclear. Therefore, goal this paper investigate for first time process mitochondrial network stemness restoration using unique model isolated SAM/P6 mice model. Methods were healthy BALB/c mice. We analysed impact on expression crucial markers related cells’ viability, reconstruction autophagy progression. Further, we established homeostasis, as well defined composition extracellular matrix osteogenic cultures. The miR-21 vivo also investigated critical-size cranial defect by computed microtomography SEM–EDX imaging. Results MiR-21 upregulation improved viability drove dynamics evidenced intensification fission processes. Simultaneously, enhanced differentiation increased Runx-2 downregulated Trap , calcification matrix. Importantly, analyses indicated greater ratio newly formed tissue after application, upregulated content calcium phosphorus within site. Conclusions Our results demonstrate that regulates fusion processes facilitates BMSCs. At same time, it enhances RUNX-2, while reduces TRAP accumulation phenotype. may bring novel molecular strategy diagnostics treatment.

Language: Английский

---

Evaluation of magnetic hyperthermia, drug delivery and biocompatibility (bone cell adhesion and zebrafish assays) of trace element co-doped hydroxyapatite combined with Mn–Zn ferrite for bone tissue applications

RSC Advances, Journal Year: 2024, Volume and Issue: 14(40), P. 29242 - 29253

Published: Jan. 1, 2024

The treatment and regeneration of bone defects, especially tumor-induced is an issue in clinical practice remains a major challenge for substitute material invention.

Language: Английский

---

Electrospun naringin-loaded microsphere/sucrose acetate isobutyrate system promotes macrophage polarization toward M2 and facilitates osteoporotic bone defect repair

Regenerative Biomaterials, Journal Year: 2023, Volume and Issue: 10

Published: Jan. 1, 2023

Abstract Repairing osteoporotic bone defects is still a major clinical challenge. Recent studies have revealed that immune response also essential in osteogenesis. The intrinsic inflammatory of the host, especially M1/M2 polarization status and secretory function macrophages, can directly affect osteogenic differentiation. Therefore, this study, an electrospun naringin-loaded microspheres/sucrose acetate isobutyrate (Ng-m-SAIB) system was constructed to investigate its effect on macrophage defects. results both vitro vivo experiments showed Ng-m-SAIB had good biocompatibility could promote toward M2, thereby forming favorable microenvironment for animal osteogenesis critical size skull model mouse (the senescence-accelerated mouse-strain P6). Together, these collectively suggested might be promising biomaterial treat with osteo-immunomodulatory effects.

Language: Английский

---

Mimic miRNA and Anti-miRNA Activated Scaffolds as a Therapeutic Strategy to Promote Bone, Cartilage, and Skin Regeneration

Macromol—A Journal of Macromolecular Research, Journal Year: 2024, Volume and Issue: 4(2), P. 165 - 189

Published: April 1, 2024

MiRNA-based therapies represent an innovative and promising strategy applicable to various medical fields, such as tissue regeneration the treatment of numerous diseases, including cancer, cardiovascular problems, viral infections. MiRNAs, a group small non-coding RNAs, play critical role in regulating gene expression at post-transcriptional level modulate several signaling pathways that maintain cellular homeostasis. The clinical trials discussed review herald new therapeutic era for miRNAs, particularly engineering, using synthetic exogenous mimic miRNAs antisense (anti-miRNAs) restore health. This provides overview miRNAs’ biogenesis, mechanism action, regulation, potential applications, followed by examination challenges associated with transport delivery miRNAs. possibility non-viral vectors protect against degradation ensure effective miRNA is highlighted, focusing on advantages emerging use 3D biomaterial scaffolds anti-miRNAs facilitate repair regeneration. Finally, assesses current landscape miRNA-activated scaffold preclinical studies bone, cartilage, skin tissues, emphasizing their emergence frontier personalized medicine.

Language: Английский

---

Tailored thymoquinone intercalated Layered Double Hydroxide (LDH) nanocomposites to accelerate mineralization for enhanced osteogenesis

Applied Clay Science, Journal Year: 2024, Volume and Issue: 252, P. 107339 - 107339

Published: March 16, 2024

Language: Английский

---

GelMA hydrogels reinforced by PCL@GelMA nanofibers and bioactive glass induce bone regeneration in critical size cranial defects

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 11, 2024

The process of bone healing is complex and involves the participation osteogenic stem cells, extracellular matrix, angiogenesis. advancement regeneration materials provides a promising opportunity to tackle defects. This study introduces composite hydrogel that can be injected cured using UV light. Hydrogels comprise bioactive glass (BG) PCL@GelMA coaxial nanofibers. addition BG nanofibers improves hydrogel's mechanical capabilities (353.22 ± 36.13 kPa) stability while decreasing its swelling (258.78 17.56%) hydration (72.07 1.44%) characteristics. demonstrates exceptional biocompatibility angiogenesis, enhances development in marrow mesenchymal cells (BMSCs), dramatically increases expression critical markers such as ALP, RUNX2, OPN. significantly (25.08 1.08%) non-healing calvaria defects promotes increased both marker (OPN) angiogenic (CD31) vivo. OPN CD31 was up 5 1.87 times higher than control group at 12 weeks. We successfully prepared novel injectable hydrogel, design hydrogels shows significant potential for enhancing biocompatibility, improving expression, has beneficial effect on producing an optimal microenvironment repair.

Language: Английский

---

Developed Bone Biomaterials Incorporated with MicroRNAs to Promote Bone Regeneration: A Systematic Review, Bioinformatics, and Meta-analysis Study

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(9), P. 5186 - 5204

Published: Aug. 16, 2023

This systematic review and meta-analysis focused on the effectiveness of biomaterials integrated with specific microRNAs (miRNAs) for bone fracture repair treatment. We conducted a comprehensive search PubMed, Web Science, Scopus databases, identifying 42 relevant papers up to March 2022. Hydrogel-based scaffolds were most commonly used, incorporating miRNAs like miR-26a, miR-21, miR-222, miR-26a being prevalent. The revealed significant benefits into repair, particularly in hydrogel scaffolds. However, some controversies observed among studies, presenting challenges selecting appropriate this purpose. study concludes that enhances regeneration, but further trials comparing different are necessary validate their potential applications tissue regeneration.

Language: Английский

---

Enhancing the effectiveness of Alkaline Phosphatase and bone matrix proteins by tunable metal-organic composite for accelerated mineralization

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 252, P. 126524 - 126524

Published: Aug. 24, 2023

Language: Английский

---

A Systematic Review: Comparison of Immunocytochemistry, ELISA, and Western Blot Methods in Alkaline phosphatase Measurement at Genistein-induced Osteoblast Cell

Biomedical & Pharmacology Journal, Journal Year: 2022, Volume and Issue: 15(4), P. 1853 - 1865

Published: Dec. 20, 2022

Osteoporosis is a bone disorder characterized by the decrease of mass along with micro-architecture damage and has risk become fracture. One causes osteoporosis estrogen deficiency. Genistein phytoestrogen compound in isoflavone group containing similar structure compared to 17β-estradiol, thus it can bind receptors produce an estrogenic effect. induction stimulate formation promote increase alkaline phosphate (ALP) activities osteoblast cells which be observed immunocytochemistry or Enzyme-linked Immunosorbent Assay (ELISA) Western blot method. Using PRISMA guideline technique, choose strategize article searches reading title, abstract, then whole text article. Articles keywords "genistein immunofluorescence ELISA western blot" were retrieved from databases including Google Scholar, PubMed, Researchgate, Sciencedirect. 24 relevant research articles uncovered as result this systematic review. Comparison methods order analyze ALP induced genistein includes selectivity, sensitivity, processing time, cost efficiency parameters. The method higher level sensitivity faster whereas selectivity less efficiency. for detecting complex-level protein expression.

Language: Английский

---