Recent Nanotechnologies to Overcome the Bacterial Biofilm Matrix Barriers

Small, Journal Year: 2022, Volume and Issue: 19(6)

Published: Dec. 5, 2022

Abstract Bacterial biofilm‐related infectious diseases severely influence human health. Under typical situations, pathogens can colonize inert or biological surfaces and form biofilms. Biofilms are functional aggregates that coat bacteria with extracellular polymeric substances (EPS). The main reason for the failure of biofilm infection treatment is low permeability enrichment therapeutic agents within biofilm, which results from particular features matrix barriers such as negatively charged components highly viscous compact EPS structures. Hence, developing novel strategies enhanced penetrability crucial. Herein, current progress nanotechnology methods to improve agents’ against matrix, regulating material morphology surface properties, utilizing physical penetration nano/micromotors microneedle patches, equipping nanoparticles degradation enzymes signal molecules, first summarized. Finally, challenges, perspectives, future implementations engineered delivery systems manage infections presented in detail.

Language: Английский

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Nanohybrid Double Network Hydrogels Based on a Platinum Nanozyme Composite for Antimicrobial and Diabetic Wound Healing

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(14), P. 17612 - 17626

Published: April 3, 2023

Along with hypoxia, severe bacterial infection, and abnormal pH, continuous inflammatory response hinders diabetic wounds from healing. It leads to the accumulation of large amounts reactive oxygen species (ROS) therefore prevents transition phase proliferative phase. In this work, a nanohybrid double network hydrogel injectable, self-healing, tissue adhesion properties based on platinum nanozyme composite (PFOB@PLGA@Pt) was constructed manage wound PFOB@PLGA@Pt exhibited supply capacity enzyme catalytic performance accompanied by pH self-regulation in entire phases first stage, carried perfluorooctyl bromide (PFOB) can ameliorate hypoxia boost glucose oxidase-like catalyzed reaction Pt NPs, leading lowered environment gluconic acid. As result, NADH oxidase-like, peroxidase-like, multiple activities were activated successively, synergistic antibacterial effects through production ROS. After infection had cleared, catalase-like superoxide dismutase-like NPs reshaped redox microenvironment scavenging excess ROS, which transitioned The microenvironmentally adaptive treatment cover all healing, showing significant promoting effect repair infected wounds.

Language: Английский

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Biofilm Resilience: Molecular Mechanisms Driving Antibiotic Resistance in Clinical Contexts

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 165 - 165

Published: Feb. 6, 2025

Healthcare-associated infections pose a significant global health challenge, negatively impacting patient outcomes and burdening healthcare systems. A major contributing factor to healthcare-associated is the formation of biofilms, structured microbial communities encased in self-produced extracellular polymeric substance matrix. Biofilms are critical disease etiology antibiotic resistance, complicating treatment infection control efforts. Their inherent resistance mechanisms enable them withstand therapies, leading recurrent increased morbidity. This review explores development biofilms their dual roles disease. It highlights structural protective functions EPS matrix, which shields populations from immune responses antimicrobial agents. Key molecular biofilm including restricted penetration, persister cell dormancy, genetic adaptations, identified as barriers effective management. implicated various clinical contexts, chronic wounds, medical device-associated infections, oral complications, surgical site infections. prevalence hospital environments exacerbates challenges underscores urgent need for innovative preventive therapeutic strategies. evaluates cutting-edge approaches such DNase-mediated disruption, RNAIII-inhibiting peptides, DNABII proteins, bacteriophage nanoparticle-based solutions, coatings, lock therapies. also examines associated with biofilm-related diagnostic difficulties, disinfectant economic implications. emphasizes multidisciplinary approach importance understanding dynamics, role pathogenesis, advancements strategies combat biofilm-associated effectively settings. These insights aim enhance reduce burden diseases.

Language: Английский

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Stimuli-responsive therapeutic systems for the treatment of diabetic infected wounds

Nanoscale, Journal Year: 2022, Volume and Issue: 14(36), P. 12967 - 12983

Published: Jan. 1, 2022

Diabetic wound infection is a common disease that has significantly reduced people's quality of life. Although tremendous achievements have been made in clinical treatment, the crucial challenge diabetic infected management stems from detrimental environment and emergence bacterial resistance after long-term medication, which result efficacy, an increased dosage severe side effects. To tackle these issues, it great significance to develop innovative treatment strategy for therapy. Currently, exploitation nanobiomaterial-based therapeutic systems wounds booming, with stimuli-responsive performance received extensive attention. These are able accelerate healing due on-demand release agents response stimulating factors. Based on characteristics wounds, many endogenous (e.g., glucose, enzyme, hypoxia, acidity) employed targeted patients. Additionally, exogenous stimulants, including light, magnetism, temperature, also capable achieving drug activation. In this review, presented, then exogenous/endogenous stimuli summarized. Finally, current challenges future outlook discussed.

Language: Английский

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Biofilm microenvironment response nanoplatform synergistically degrades biofilm structure and relieves hypoxia for efficient sonodynamic therapy

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 453, P. 139839 - 139839

Published: Oct. 19, 2022

Treatment of bacterial biofilms remains a great challenge in the clinic. Recently, ultrasound (US)-driven antimicrobial sonodynamic therapy (aSDT) has been considered as an emerging therapeutic strategy for treatment biofilm infections. However, hypoxic microenvironment and restricted diffusion sonosensitizers within substantially reduce efficacy aSDT. Herein, microenvironment-responsive nanoplatform was proposed to promote degradation sonosensitizer penetration, relieve microenvironment, thereby augmenting aSDT efficiency against This prepared by modifying manganese dioxide nanosheets (MNS) with α-amylase, polyethylene glycol (PEG), chlorin e6 (Ce6) form MNS-α-amylase/PEG-Ce6 (MAPC). After delivery into biofilm-infected tissues, MAPC decompose acidic locally release α-amylase Ce6. The degrades extracellular polymeric substances Ce6 penetration. In addition, MNS catalyze conversion endogenously overproduced H2O2 O2 infected tissue further enhance antibiofilm Such hypoxia-relief enhanced show approximately 6.9 log units (99.99998%) reduction bacteria vitro, efficiently treat methicillin-resistant Staphylococcus aureus (MRSA) biofilms-infected mice. Overall, improves penetration relieves effects MAPC. Thus, use this provides promising combating biofilm-associated

Language: Английский

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Recent advances in nanozymes for combating bacterial infection

Materials Chemistry Frontiers, Journal Year: 2022, Volume and Issue: 6(18), P. 2596 - 2609

Published: Jan. 1, 2022

In this review, we summarize the recent advance of nanozymes in treatment bacterial infection.

Language: Английский

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Environment‐Responsive Therapeutic Platforms for the Treatment of Implant Infection

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(26)

Published: May 15, 2023

Abstract The application of medical implants has greatly improved the survival rate and life quality patients. Nevertheless, in recent years, there are increasing cases implant dysfunction or failure because bacterial infections. Despite significant improvements biomedicine, still serious challenges treatment implant‐related With formation biofilms development resistance, these limitations lead to a low efficacy conventional antibiotics. To address challenges, it is urgent exploit innovative strategies for Based on ideas, environment‐responsive therapeutic platforms with high selectivity, drug minor dose‐limiting toxicity have attracted widespread attention. By using exogenous/endogenous stimuli, antibacterial activity therapeutics can be activated demand exhibit remarkable effects. Exogenous stimuli include photo, magnetism, microwave, ultrasound. Endogenous mainly pathological characteristics infections such as acidic pH, anomalous temperature, abnormal enzymatic activities. In this review, progress spatiotemporally controlled release/activation systematically summarized. Afterward, opportunities emerging highlighted. Finally, hoped that review will offer novel ideas techniques combat

Language: Английский

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Self-Adaptive Antibiofilm Effect and Immune Regulation by Hollow Cu₂MoS₄ Nanospheres for Treatment of Implant Infections

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(15), P. 18720 - 18733

Published: April 5, 2023

Implant infections are difficult to cure by traditional antibiotic therapy due bacterial biofilm-induced tolerance and impaired immune responses. To efficiently treat implant infections, therapeutic agents need kill bacteria regulate the inflammatory response of cells during biofilm elimination process. Herein, multifunctional smart hollow Cu2MoS4 nanospheres (H-CMS NSs) with pH-responsive enzyme-like activities were prepared for self-adaptively eliminating biofilms regulating inflammation macrophages in infections. During infection, tissue microenvironment around implants is acidic. H-CMS NSs oxidase (OXD)/peroxidase (POD)-like can catalyze reactive oxidative species (ROS) generation directly killing polarizing a proinflammatory phenotype. Moreover, POD-like activity antibacterial property be further enhanced under ultrasound (US) irradiation. After biofilms, shifts from acidic neutral. show catalase (CAT)-like eliminate excessive ROS, which polarizes anti-inflammatory phenotype promotes healing infected tissue. This work provides nanozyme self-adaptive regulation antibiofilm ROS generation/elimination according different pathological microenvironments stages.

Language: Английский

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Flexible nanoplatform facilitates antibacterial phototherapy by simultaneously enhancing photosensitizer permeation and relieving hypoxia in bacterial biofilms

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 184, P. 313 - 322

Published: June 17, 2024

Antimicrobial phototherapy has gained recognition as a promising approach for addressing bacterial biofilms, however, its effectiveness is often impeded by the robust physical and chemical defenses of biofilms. Traditional antibacterial nanoplatforms face challenges in breaching extracellular polymeric substances barrier to efficiently deliver photosensitizers deep into Moreover, prevalent hypoxia within biofilms restricts success oxygen-reliant phototherapy. In this study, we engineered soft mesoporous organosilica nanoplatform (SMONs) incorporating polyethylene glycol (PEG), catalase (CAT), indocyanine green (ICG), forming SMONs-PEG-CAT-ICG (SPCI). We compared antimicrobial efficacy SPCI with more rigid nanoplatforms. Our results demonstrated that unique flexible mechanical properties enable it navigate through biofilm barriers, markedly enhancing ICG penetration methicillin-resistant Staphylococcus aureus (MRSA) Notably, murine subcutaneous MRSA infection model, showed superior pharmacokinetic benefits over counterparts. The embedded effectively converts excess H

Language: Английский

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Copper-based nanomaterials for biomedical applications

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 483, P. 149040 - 149040

Published: Jan. 23, 2024

Among metal-based nanomaterials, Copper-based nanomaterials (Cu-BNMs) have become a research hotspot in the biomedical field due to their good biocompatibility and unique properties. However, there are fewer systematic summaries of copper-based especially synthesis Cu-BNMs. As for applications, Cu-BNMs been widely used fields antimicrobial, imaging, therapeutics, showing excellent promising Herein, this review firstly introduces classification various methods synthesizing through both top-down bottom-up types. Then, applications within three tumor therapy classified summarized. Finally, challenges faced application future development c discussed provide basic information illustrations

Language: Английский

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