Morphological, Pathological and Phylogenetic Analyses of Phytophthora heterospora Causing Durian Stem Canker Disease in the Eastern Coast of Peninsular Malaysia DOI

Muhammad Iqbal Hakim Johari,

Dzarifah Zulperi, Norsazilawati Saad

et al.

Plant Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

ABSTRACT Durian ( Durio zibethinus ), a highly popular tropical fruit in Southeast Asia, faces major challenge from stem canker disease, primarily caused by oomycetes. This study aimed to identify and characterise the causal agent of durian on eastern coast Peninsular Malaysia. A combination morphological, pathological phylogenetic analyses was employed comprehensively understand pathogen's characteristics. series samplings conducted several orchards. Isolates were identified using morphological characteristics, DNA sequences analysis multiple markers, namely internal transcribed spacer (ITS) region ribosomal RNA gene cluster, β‐tubulin BTUB ) cytochrome c oxidase subunit I COXI ). Three representative isolates selected for pathogenicity tests seedlings. The sampling sites had disease incidence ranging 10% 54%. total 25 recovered symptomatic tissues showed features that resembled those Phytophthora species. Phylogenetic concatenated markers put all same group as heterospora , supported with 98% maximum‐likelihood bootstrap values. result revealed P . durian.

Language: Английский

What are the 100 most cited fungal genera? DOI

C.S. Bhunjun,

Yijun Chen, Chayanard Phukhamsakda

et al.

Studies in Mycology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

23

Worldwide forest surveys reveal forty-three new species in Phytophthora major Clade 2 with fundamental implications for the evolution and biogeography of the genus and global plant biosecurity DOI Creative Commons
Thomas Jung, Ivan Milenković, Y. Balcì

et al.

Studies in Mycology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

New species: Phytophthora amamensis T. Jung, K. Kageyama, H. Masuya & S. Uematsu, angustata L. Garcia, B. Mendieta-Araica, Y. Balci, balkanensis I. Milenković, Ž. Tomić, Jung M. Horta borneensis A. Durán, Tarigan calidophila Garcia catenulata T.-T. Chang, N.M. Chi celeris Oliveira, curvata Hieno, distorta E. Sanfuentes von Stowasser excentrica Kageyama C.M. Brasier, falcata Uematsu fansipanensis Chi, Corcobado frigidophila Broders furcata Milenković inclinata indonesiensis Tarigan, Oliveira japonensis J.F. Webber, limosa Corcobado, Majek, Ferreira macroglobulosa H.-C. Zeng, H.-H. Ho, F.-C. Zheng montana multipapillata multiplex nimia Masuya, Hieno oblonga obovoidea obturata penetrans platani Pérez-Sierra, S.O. Cacciola proliferata pseudocapensis pseudocitrophthora Cacciola, J. Bakonyi pseudofrigida pseudoccultans pyriformis K.D. Boders sumatera Junaid transposita Brasier vacuola valdiviana Stowasser, Durán variepedicellata vietnamensis ×australasiatica ×lusitanica C. Maia ×taiwanensis H.-S. Fu Jung.

Citations

22

Challenges to Mediterranean Fagaceae ecosystems affected by Phytophthora cinnamomi and Climate Change: Integrated Pest Management perspectives DOI
Carmen Morales‐Rodríguez, Andrea Vannini, Bruno Scanu

et al.

Current Forestry Reports, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 14, 2025

Language: Английский

Citations

2

The prevalence of Phytophthora in British plant nurseries; high‐risk hosts and substrates and opportunities to implement best practice DOI Creative Commons
Sarah Green, D. E. L. Cooke, Louise Barwell

et al.

Plant Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 20, 2025

Abstract Invasive Phytophthora species infect a very broad range of herbaceous and woody hosts globally. The UK alone has experienced particularly damaging series outbreaks epidemics new, invasive affecting the nation's trees over last 30 years. link between importation spread infected nursery stock is well established across many countries worldwide. To understand better pathways in trade Britain, we applied standardized sampling method combined with refined metabarcoding detection to capture diversity at 134 British plant nurseries representing biosecurity trading practices multiple years 2016 2022. This included root water samples collected from 17 sampled seasonally 117 once as part health inspections. Based on analyses 1894 pooled samples, DNA barcodes 85 or complexes were detected, variation species' relative frequencies nurseries. We present top 20 host– associations ranked by frequency report five novel records for UK. identified surprisingly high‐risk (such Douglas fir) greatest number revealed niche preferences roots. discuss implications our findings terms pathogen abundance, hosts, information dissemination approach resulting advice aimed reducing risk.

Language: Английский

Citations

1

Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques DOI Creative Commons
Aneta Bačová, D. E. L. Cooke, Ivan Milenković

et al.

European Journal of Plant Pathology, Journal Year: 2024, Volume and Issue: 170(1), P. 131 - 156

Published: June 17, 2024

Abstract Phytophthora diversity was examined in eight forest and ornamental nurseries the Czech Republic. A leaf baiting isolation technique and, two nurseries, also Illumina DNA metabarcoding were used to reveal of soil irrigation water compare efficacy both approaches. In total, revealed occurrence 12 taxa 59.4% samples from seven (87.5%) nurseries. Additional compost carried out species recovered. Irrigation three by or direct partially decomposed floating leaves collected source, obtained. sequencing done one respectively. reads identified as 45 taxa, 15 them previously unknown Clades 6, 7, 8 9. Another 11 belonged known undescribed oomycete genera Globisporangium , Hyaloperonospora Nothophytophthora Peronospora Plasmopara . Overall, with techniques 50 detected five ( P. taxon organica, plurivora, rosacearum, syringae transitoria ) being exclusively 38 only metabarcoding. Particularly common records barcoding cinnamomi lateralis which not isolated baiting. Only techniques. It is recommended use combination determine true managed natural ecosystems presence rare taxa.

Language: Английский

Citations

6

High diversity of Phytophthora species in natural ecosystems and nurseries of Portugal: Detrimental side effect of plant introductions from the age of discovery to modern globalization DOI Creative Commons
Marília Horta Jung, C. M. B. de F. Maia, Beatriz Mora‐Sala

et al.

Plant Pathology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 23, 2024

Abstract Between 2010 and 2015, an extensive survey of Phytophthora diversity was performed across Portugal. Baiting direct isolation tests obtained 48 taxa in 62 70 forest stands (89%) from 148 302 rhizosphere soil samples (49%) 25 29 tree species (86%), bark cankers Alnus lusitanica Fagus sylvatica ; 33 34 rivers streams (97%); 12 13 nurseries (92%) 54 87 root ball (61%) 16 21 (76%). The 799 isolates belonged to 28 known three recently described species; one designated two new non‐hybrid taxa; five nine hybrid taxa. majority, (71%), are introduced aliens Europe while (19%) (10%) cryptic native origin, respectively. cinnamomi the most common forests (56%) (61%). Multiple first records for Portugal partly both declines host– associations were found. Isolation rates differed considerably between declining (65%) healthy trees (10%), regardless species. widespread infestations with aggressive wide‐host range pathogens like P . , pseudocryptogea × cambivora multivora plurivora host‐specific alticola quercetorum quercina will inevitably cause their further spread wider environment, exacerbating pathogen threats ecosystems thus weakening resilience climatic extremes.

Language: Английский

Citations

5

Phytophthora nicotianae and Ph. mediterranea: A biosecurity threat to Platanus orientalis and P. x acerifolia in urban green areas in Greece DOI Creative Commons
Chiara Antonelli, Nikoleta Soulioti, Benedetto Teodoro Linaldeddu

et al.

Urban forestry & urban greening, Journal Year: 2024, Volume and Issue: 95, P. 128281 - 128281

Published: March 15, 2024

Language: Английский

Citations

4

Caracterización molecular de Phytophthora cinnamomi causante de la pudrición radical de Annona cherimola L. en la costa central del Perú DOI Open Access
Yuri H. Calle-Cheje, René Aguilar-Anccota, Robert Rafael-Rutte

et al.

Bioagro, Journal Year: 2025, Volume and Issue: 37(1), P. 39 - 52

Published: Jan. 1, 2025

La Chirimoya (Annona cherimola Mill.) es una planta que se cultiva principalmente en los valles interandinos de la región Lima, Perú, presentan condiciones adecuadas para su producción. Sin embargo, estas condiciones, también, favorecen el desarrollo patógenos causan decaimiento y muerte las plantas chirimoya. El objetivo del estudio fue identificar molecularmente agente causal pudrición raíz Se recolectaron raicillas chirimoya con síntomas leves severos valles: Huanangui, San Antonio Cumbe Huaral, Lima. En medio PDA V8 obtuvieron siete aislados Phytophthora micelio coraloide, petiolado, hifas cenocíticas hinchamientos hifales, esporangios obpiriformes, ovoide sin papilas clamidosporas globosas. Las regiones subunidad II citocromo oxidasa (Cox2) factor elongación alfa 1 (EF1-α) amplificaron utilizando primers FM75, FM78, EF1AF EF1AR. secuencias obtenidas mostraron similitud 99,22 al 99,87 % Cox2 99,04 100 EF1-α cinnamomi. árbol filogenético mostró topología congruente accesiones Phytophthora. concluye P. cinnamomi causante raíz, siendo este primer reporte sobre presencia patógeno Perú.

Citations

0

Evolutionary Relationships of the Phytophthora 1a Subclade Species Based on Complete Mitogenomes, and Novel Markers for Their Differentiation DOI Creative Commons

Tao Lin,

Huiqin Wang,

Haiting Ma

et al.

Ecology and Evolution, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

ABSTRACT Phytophthora is a genus of oomycetes that includes many aggressive pathogens capable devastating farmlands and forests worldwide. Among the oldest most well‐known species, P. cactorum exhibits morphological genetic similarities to other homothallic species within subclade 1a, which complicates understanding their evolutionary relationships. This study primarily compared seven strains from diverse origins with three closely related in utilizing mitogenome sequences for analysis. The circular mitogenomes four were nearly identical size comprised 38 protein‐coding genes (PCGs), 25 transfer ribonucleic acid genes, 2 ribosomal RNA genes. mitochondrial genomes exhibited higher percentage A/T G/C content. majority AT‐skew GC‐skew values among PCGs positive, demonstrating more pronounced bias than GC‐skew. Ka/Ks ratios revealed 35 underwent significant purifying selection. Although AliGROOVE analysis indicated notable 1a significantly pairing frequency complete mitogenome. results phylogenetic aligned pairwise distances, indicating pseudotsugae hedraiandra . Furthermore, we found nad9 gene informative differentiate akin cox1 gene.

Language: Английский

Citations

0

Morpho-cultural variability, pathogenicity and molecular identification of Phytopthora cajani isolates causing blight disease of pigeonpea in India DOI
Raj Kumar Mishra, Abhishek Bohra, Deepak Kumar

et al.

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102674 - 102674

Published: March 1, 2025

Language: Английский

Citations

0