Plant Pathology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 27, 2025
ABSTRACT
Durian
(
Durio
zibethinus
),
a
highly
popular
tropical
fruit
in
Southeast
Asia,
faces
major
challenge
from
stem
canker
disease,
primarily
caused
by
oomycetes.
This
study
aimed
to
identify
and
characterise
the
causal
agent
of
durian
on
eastern
coast
Peninsular
Malaysia.
A
combination
morphological,
pathological
phylogenetic
analyses
was
employed
comprehensively
understand
pathogen's
characteristics.
series
samplings
conducted
several
orchards.
Isolates
were
identified
using
morphological
characteristics,
DNA
sequences
analysis
multiple
markers,
namely
internal
transcribed
spacer
(ITS)
region
ribosomal
RNA
gene
cluster,
β‐tubulin
BTUB
)
cytochrome
c
oxidase
subunit
I
COXI
).
Three
representative
isolates
selected
for
pathogenicity
tests
seedlings.
The
sampling
sites
had
disease
incidence
ranging
10%
54%.
total
25
recovered
symptomatic
tissues
showed
features
that
resembled
those
Phytophthora
species.
Phylogenetic
concatenated
markers
put
all
same
group
as
heterospora
,
supported
with
98%
maximum‐likelihood
bootstrap
values.
result
revealed
P
.
durian.
Plant Pathology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 20, 2025
Abstract
Invasive
Phytophthora
species
infect
a
very
broad
range
of
herbaceous
and
woody
hosts
globally.
The
UK
alone
has
experienced
particularly
damaging
series
outbreaks
epidemics
new,
invasive
affecting
the
nation's
trees
over
last
30
years.
link
between
importation
spread
infected
nursery
stock
is
well
established
across
many
countries
worldwide.
To
understand
better
pathways
in
trade
Britain,
we
applied
standardized
sampling
method
combined
with
refined
metabarcoding
detection
to
capture
diversity
at
134
British
plant
nurseries
representing
biosecurity
trading
practices
multiple
years
2016
2022.
This
included
root
water
samples
collected
from
17
sampled
seasonally
117
once
as
part
health
inspections.
Based
on
analyses
1894
pooled
samples,
DNA
barcodes
85
or
complexes
were
detected,
variation
species'
relative
frequencies
nurseries.
We
present
top
20
host–
associations
ranked
by
frequency
report
five
novel
records
for
UK.
identified
surprisingly
high‐risk
(such
Douglas
fir)
greatest
number
revealed
niche
preferences
roots.
discuss
implications
our
findings
terms
pathogen
abundance,
hosts,
information
dissemination
approach
resulting
advice
aimed
reducing
risk.
European Journal of Plant Pathology,
Journal Year:
2024,
Volume and Issue:
170(1), P. 131 - 156
Published: June 17, 2024
Abstract
Phytophthora
diversity
was
examined
in
eight
forest
and
ornamental
nurseries
the
Czech
Republic.
A
leaf
baiting
isolation
technique
and,
two
nurseries,
also
Illumina
DNA
metabarcoding
were
used
to
reveal
of
soil
irrigation
water
compare
efficacy
both
approaches.
In
total,
revealed
occurrence
12
taxa
59.4%
samples
from
seven
(87.5%)
nurseries.
Additional
compost
carried
out
species
recovered.
Irrigation
three
by
or
direct
partially
decomposed
floating
leaves
collected
source,
obtained.
sequencing
done
one
respectively.
reads
identified
as
45
taxa,
15
them
previously
unknown
Clades
6,
7,
8
9.
Another
11
belonged
known
undescribed
oomycete
genera
Globisporangium
,
Hyaloperonospora
Nothophytophthora
Peronospora
Plasmopara
.
Overall,
with
techniques
50
detected
five
(
P.
taxon
organica,
plurivora,
rosacearum,
syringae
transitoria
)
being
exclusively
38
only
metabarcoding.
Particularly
common
records
barcoding
cinnamomi
lateralis
which
not
isolated
baiting.
Only
techniques.
It
is
recommended
use
combination
determine
true
managed
natural
ecosystems
presence
rare
taxa.
Plant Pathology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 23, 2024
Abstract
Between
2010
and
2015,
an
extensive
survey
of
Phytophthora
diversity
was
performed
across
Portugal.
Baiting
direct
isolation
tests
obtained
48
taxa
in
62
70
forest
stands
(89%)
from
148
302
rhizosphere
soil
samples
(49%)
25
29
tree
species
(86%),
bark
cankers
Alnus
lusitanica
Fagus
sylvatica
;
33
34
rivers
streams
(97%);
12
13
nurseries
(92%)
54
87
root
ball
(61%)
16
21
(76%).
The
799
isolates
belonged
to
28
known
three
recently
described
species;
one
designated
two
new
non‐hybrid
taxa;
five
nine
hybrid
taxa.
majority,
(71%),
are
introduced
aliens
Europe
while
(19%)
(10%)
cryptic
native
origin,
respectively.
cinnamomi
the
most
common
forests
(56%)
(61%).
Multiple
first
records
for
Portugal
partly
both
declines
host–
associations
were
found.
Isolation
rates
differed
considerably
between
declining
(65%)
healthy
trees
(10%),
regardless
species.
widespread
infestations
with
aggressive
wide‐host
range
pathogens
like
P
.
,
pseudocryptogea
×
cambivora
multivora
plurivora
host‐specific
alticola
quercetorum
quercina
will
inevitably
cause
their
further
spread
wider
environment,
exacerbating
pathogen
threats
ecosystems
thus
weakening
resilience
climatic
extremes.
Bioagro,
Journal Year:
2025,
Volume and Issue:
37(1), P. 39 - 52
Published: Jan. 1, 2025
La
Chirimoya
(Annona
cherimola
Mill.)
es
una
planta
que
se
cultiva
principalmente
en
los
valles
interandinos
de
la
región
Lima,
Perú,
presentan
condiciones
adecuadas
para
su
producción.
Sin
embargo,
estas
condiciones,
también,
favorecen
el
desarrollo
patógenos
causan
decaimiento
y
muerte
las
plantas
chirimoya.
El
objetivo
del
estudio
fue
identificar
molecularmente
agente
causal
pudrición
raíz
Se
recolectaron
raicillas
chirimoya
con
síntomas
leves
severos
valles:
Huanangui,
San
Antonio
Cumbe
Huaral,
Lima.
En
medio
PDA
V8
obtuvieron
siete
aislados
Phytophthora
micelio
coraloide,
petiolado,
hifas
cenocíticas
hinchamientos
hifales,
esporangios
obpiriformes,
ovoide
sin
papilas
clamidosporas
globosas.
Las
regiones
subunidad
II
citocromo
oxidasa
(Cox2)
factor
elongación
alfa
1
(EF1-α)
amplificaron
utilizando
primers
FM75,
FM78,
EF1AF
EF1AR.
secuencias
obtenidas
mostraron
similitud
99,22
al
99,87
%
Cox2
99,04
100
EF1-α
cinnamomi.
árbol
filogenético
mostró
topología
congruente
accesiones
Phytophthora.
concluye
P.
cinnamomi
causante
raíz,
siendo
este
primer
reporte
sobre
presencia
patógeno
Perú.
Ecology and Evolution,
Journal Year:
2025,
Volume and Issue:
15(3)
Published: March 1, 2025
ABSTRACT
Phytophthora
is
a
genus
of
oomycetes
that
includes
many
aggressive
pathogens
capable
devastating
farmlands
and
forests
worldwide.
Among
the
oldest
most
well‐known
species,
P.
cactorum
exhibits
morphological
genetic
similarities
to
other
homothallic
species
within
subclade
1a,
which
complicates
understanding
their
evolutionary
relationships.
This
study
primarily
compared
seven
strains
from
diverse
origins
with
three
closely
related
in
utilizing
mitogenome
sequences
for
analysis.
The
circular
mitogenomes
four
were
nearly
identical
size
comprised
38
protein‐coding
genes
(PCGs),
25
transfer
ribonucleic
acid
genes,
2
ribosomal
RNA
genes.
mitochondrial
genomes
exhibited
higher
percentage
A/T
G/C
content.
majority
AT‐skew
GC‐skew
values
among
PCGs
positive,
demonstrating
more
pronounced
bias
than
GC‐skew.
Ka/Ks
ratios
revealed
35
underwent
significant
purifying
selection.
Although
AliGROOVE
analysis
indicated
notable
1a
significantly
pairing
frequency
complete
mitogenome.
results
phylogenetic
aligned
pairwise
distances,
indicating
pseudotsugae
hedraiandra
.
Furthermore,
we
found
nad9
gene
informative
differentiate
akin
cox1
gene.