Microbiota of the Pregnant Mouse: Characterization of the Bacterial Communities in the Oral Cavity, Lung, Intestine, and Vagina through Culture and DNA Sequencing DOI Creative Commons
Jonathan M. Greenberg, Roberto Romero, Andrew D. Winters

et al.

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(4)

Published: Aug. 2, 2022

Mice are frequently used as animal models for mechanistic studies of infection and obstetrical disease, yet characterization the murine microbiota during pregnancy is lacking. The objective this study was to characterize microbiotas distinct body sites pregnant mouse-vagina, oral cavity, intestine, lung-that harbor microorganisms that could potentially invade amniotic thus leading adverse outcomes. these were characterized through anoxic, hypoxic, oxic culture well 16S rRNA gene sequencing. With exception vagina, cultured each site varied by atmosphere, with greatest diversity in appearing under anoxic conditions. Only cultures vagina comprehensively representative observed direct DNA sequencing samples, primarily due predominance two Rodentibacter strains. Identified pneumotropicus heylii, isolates exhibited patterns similar those Lactobacillus crispatus iners human vagina. Whole-genome strains revealed shared genomic features, including ability degrade glycogen, an abundant polysaccharide In summary, we report site-specific mouse potential ecological parallels humans. Importantly, our findings indicate vaginal mice can be readily cultured, suggesting mock tractably generated maintained experimental manipulation future host vaginal-microbiome interactions. IMPORTANCE widely utilized complications; however, has been neglected. Microorganisms from lung have found intra-amniotic space, where their presence threatens progression gestation. Here, established appropriateness capturing at site. high relative abundance humans, parallels. hypoxic conditions, demonstrating microbial communities test between evaluate relevance structure outcomes, especially preterm birth.

Language: Английский

The vaginal microbiome and the risk of preterm birth: a systematic review and network meta-analysis DOI Creative Commons
Unnur Guðnadóttir, Justine W. Debelius, Juan Du

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: May 13, 2022

Abstract Preterm birth is a major cause of neonatal morbidity and mortality worldwide. Increasing evidence links the vaginal microbiome to risk spontaneous preterm labour that leads birth. The aim this systematic review network meta-analysis was investigate association between microbiome, defined as community state types (CSTs, i.e. dominance specific lactobacilli spp, or not (low-lactobacilli)), Systematic using PubMed, Web Science, Embase Cochrane library performed. Longitudinal studies culture-independent methods categorizing in at least three different CSTs assess were included. A (network) conducted, presenting pooled odds ratios (OR) 95% confidence intervals (CI); weighted proportions CI. All 17 published 2014 2021 included 38–539 pregnancies 8–107 births. Women with “low-lactobacilli” increased (OR 1.69, CI 1.15–2.49) for delivering compared Lactobacillus crispatus dominant women. Our supports being predictive birth, where low abundance associated highest risk, L. lowest.

Language: Английский

Citations

78
Microbial diversity in the vaginal microbiota and its link to pregnancy outcomes DOI Creative Commons

Agnes Baud,

Kenzo-Hugo Hillion, Céline Plainvert

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: June 4, 2023

Abstract The vaginal microbiota refers to the microorganisms that reside in vagina. These contribute significantly a woman’s reproductive and general health. A healthy is typically low-diversity environment with predominance of lactic acid-producing Lactobacillus species. Factors such as antibiotic use, sexual activity, hormonal changes can disrupt balance microbiota, leading conditions bacterial vaginosis. composition takes on added importance during pregnancy, serving barrier against infection for both mother fetus. Despite colonize vagina, details how diversity impact pregnancy outcomes poorly understood. This especially true woman high prevalence Gardnerella vaginalis . Here we report diverse cohort 749 women, enrolled InSPIRe cohort, their final trimester pregnancy. We show Lactobacilli , including L. crispatus are important maintaining low diversity, depletion this critical community linked preterm delivery. further demonstrate it overall not specific species, which provides best indicator risk.

Language: Английский

Citations

54
Roles of the Microbiota of the Female Reproductive Tract in Gynecological and Reproductive Health DOI
Bin Zhu, Zhi Tao,

Laahirie Edupuganti

et al.

Microbiology and Molecular Biology Reviews, Journal Year: 2022, Volume and Issue: 86(4)

Published: Oct. 12, 2022

The microbiome of the female reproductive tract defies convention that high biodiversity is a hallmark an optimal ecosystem. Although not universally true, homogeneous vaginal composed species

Language: Английский

Citations

57
Integrating compositional and functional content to describe vaginal microbiomes in health and disease DOI Creative Commons
Johanna B. Holm, Michael France,

Pawel Gajer

et al.

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: Nov. 30, 2023

Abstract Background A Lactobacillus- dominated vaginal microbiome provides the first line of defense against adverse genital tract health outcomes. However, there is limited understanding mechanisms by which modulates protection, as prior work mostly described its composition through morphologic assessment and marker gene sequencing methods that do not capture functional information. To address this gap, we developed metagenomic community state types (mgCSTs) use sequences to describe define microbiomes based on both potential. Results MgCSTs are categories classified using taxonomy potential encoded in their metagenomes. reflect unique combinations subspecies (mgSs), assemblages bacterial strains same species, within a microbiome. We demonstrate mgCSTs associated with demographics such age race, well pH Gram stain smears. Importantly, these associations varied between predominated species. subset mgCSTs, including three six Gardnerella vaginalis mgSs, mgSs L. iners , were greater likelihood vaginosis diagnosed Amsel clinical criteria. This among other features, enhanced genetic capabilities for epithelial cell attachment could facilitate cytotoxin-mediated lysis. Finally, report mgCST classifier source code provided may be adapted research community. Conclusions novel easily implemented approach reduce dimension complex datasets while maintaining uniqueness. enable investigation multiple species diversity Future investigations key unraveling pathways protection tract. our findings support hypothesis differences microbiomes, those look compositionally similar, critical considerations health. Ultimately, lead hypotheses concerning role promoting disease, identify targets prognostic, diagnostic, therapeutic strategies improve women’s

Language: Английский

Citations

29
Gestational diabetes augments group B Streptococcus infection by disrupting maternal immunity and the vaginal microbiota DOI Creative Commons
Vicki Mercado-Evans, Marlyd E. Mejia, Jacob J. Zulk

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 3, 2024

Group B Streptococcus (GBS) is a pervasive perinatal pathogen, yet factors driving GBS dissemination in utero are poorly defined. Gestational diabetes mellitus (GDM), complication marked by dysregulated immunity and maternal microbial dysbiosis, increases risk for disease. Using murine GDM model of colonization transmission, we find that mice display greater subsequently worse neonatal outcomes. Dual-RNA sequencing reveals differential adaptation to the reproductive tract, including putative glycosyltransferase (yfhO), altered host responses. immune disruptions include reduced uterine natural killer cell activation, impaired recruitment placentae, maternofetal cytokines. Lastly, observe distinct vaginal taxa associated with status invasive disease status. Here, show hosts recapitulates several clinical aspects identifies multiple bacterial drivers

Language: Английский

Citations

13
Stress and depression-associated shifts in gut microbiota: A pilot study of human pregnancy DOI Creative Commons
Therese Rajasekera, Jeffrey D. Galley, Amy R. Mackos

et al.

Brain Behavior & Immunity - Health, Journal Year: 2024, Volume and Issue: 36, P. 100730 - 100730

Published: Jan. 26, 2024

Psychosocial stress and mood-related disorders, such as depression, are prevalent vulnerability to these conditions is heightened during pregnancy. induces consequences via several mechanisms including the gut microbiota-brain axis associated signaling pathways. Previous preclinical work indicates that prenatal alters maternal microbial composition impairs offspring development. Importantly, although fecal vaginal microenvironments undergo alterations across pregnancy, we lack consensus regarding which shifts adaptive or maladaptive in presence of depression. Clinical studies interrogating relationships have identified unique taxa but been limited study design.

Language: Английский

Citations

8
Untangling Associations of Microbiomes of Pregnancy and Preterm Birth DOI
Anna Powell, Fouzia Zahid Ali Khan, Jacques Ravel

et al.

Clinics in Perinatology, Journal Year: 2024, Volume and Issue: 51(2), P. 425 - 439

Published: March 15, 2024

Language: Английский

Citations

8
The Vaginal Microbiota of Pregnant Women Varies with Gestational Age, Maternal Age, and Parity DOI Creative Commons
Roberto Romero, Kevin R. Theis, Nardhy Gómez-López

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(4)

Published: July 24, 2023

The composition of the vaginal microbiota is heavily influenced by pregnancy and may factor into complications, including spontaneous preterm birth. However, results among studies have been inconsistent due, in part, to variation sample sizes ethnicity. Thus, an association between labor continues be debated. Yet, before assessing associations labor, a robust in-depth characterization throughout specific study population under investigation required. Here, we report large longitudinal (

Language: Английский

Citations

16
Joint effects of individual socioeconomic status and residential neighborhood context on vaginal microbiome composition DOI Creative Commons
Meredith Dixon, Anne L. Dunlop, Elizabeth J. Corwin

et al.

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: Jan. 24, 2023

The vaginal microbiome is a dynamic ecosystem that important for women's health. Its composition has been associated with risk menopausal symptoms, sexually transmitted infections, gynecologic cancer, and preterm birth. Conventional factors linked these adverse health outcomes include sexual behaviors, hygiene practices, individual social factors, stress levels. However, there limited research on socio-contextual determinants, whether neighborhood context modifies the association socioeconomic factors.Socioeconomically diverse pregnant African American women in Atlanta, Georgia (n = 439) provided residential addresses first trimester swab samples, which underwent sequencing, taxonomic classification, assignment into mutually exclusive CST (community state types) via hierarchical clustering. Linear probability models were used to estimate prevalence differences (PD) associations of evaluate additive interaction maternal level education, insurance type, recruitment hospital.Factors such as higher (vs. lower) private public) insurance, hospital Lactobacillus-dominant CSTs typically better outcomes. When considering joint effects status CST, most combinations showed greater than effect among doubly exposed; however, case local income homogeneity racial homogeneity, was evidence crossover between those less-advantaged more-advantaged status. Compared at public who lived economically neighborhoods, neighborhoods had 21.9% CSTs, while less (the exposed) only an 11.7% showing (interaction term p-value 0.004).In this study, aspects experienced differently by basis their resources, exposures departure from simple additivity some factors.

Language: Английский

Citations

15
Gestational diabetes as a risk factor for GBS maternal rectovaginal colonization: a systematic review and meta-analysis DOI Creative Commons
Vicki Mercado-Evans, Jacob J. Zulk,

Zainab A. Hameed

et al.

BMC Pregnancy and Childbirth, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 20, 2024

Abstract Background Maternal rectovaginal colonization by group B Streptococcus (GBS) increases the risk of perinatal GBS disease that can lead to death or long-term neurological impairment. Factors increase carriage are incompletely understood resulting in missed opportunities for detecting risk-based clinical approaches. There is a lacking consensus on whether gestational diabetes mellitus (GDM) factor GBS. This systematic review and meta-analysis aims address current conflicting findings determine GDM should be clinically considered as maternal colonization. Methods Peer-reviewed studies provided prevalence documented vaginal and/or rectal women with without were included this analysis. From study inception October 30, 2023, we identified 6,275 relevant from EMBASE PUBMED which 19 eligible inclusion. Eligible analyzed thoroughly assessed bias modified Newcastle-Ottawa Scale interrogated representativeness comparability cohorts, quality reporting status, potential other metabolic diseases. Results synthesized using STATA 18 random-effects meta-analyses. Studies encompassed 266,706 10 different countries, periods spanning 1981 2020. Meta-analysis revealed associated 16% increased (OR 1.16, CI 1.07–1.26, P = 0.003). We also performed subgroup analyses assess independent effects pregestational vs. carriage. Pregestational (Type 1 Type 2 mellitus) was an 76% (pooled OR 1.76, 1.27–2.45, 0.0008). Conclusions achieved among previously discrepant observations demonstrated significant factors Recognition during decisions about screening intrapartum antibiotic prophylaxis may decrease global burden maternal-perinatal health.

Language: Английский

Citations

5