Tracking tripartite interaction dynamics: isolation, integration, and influence of bacteriophages in the Paraburkholderia-Dictyostelium discoideum symbiosis system DOI Creative Commons
Susanne DiSalvo, Nicholas J. Maness, Andrew P. Braun

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 2, 2025

Introduction Bacteriophages influence interactions between bacterial symbionts and their hosts by exerting parasitic pressure on symbiont populations facilitating evolution through selection, gene exchange, prophage integration. Host organisms also modulate phage-bacteria interactions, with host-specific contexts potentially limiting or promoting phage access to driving alternative phenotypic evolutionary outcomes. Methods To better elucidate tripartite phage-bacteria-host in real-time, we expanded the Dictyostelium discoideum-Paraburkholderia symbiosis system include Paraburkholderia -specific phages. We isolated six environmental phages from soil samples using a multi-host enrichment approach. identified functional monocultures of one strains implemented These were evaluated across all three amoeba-associated species. Finally, treated infected amoeba lines select isolates assessed effects prevalence host fitness. Results The exhibited diverse plaquing characteristics virion morphologies, collectively targeting belonging each amoeba-symbiotic Following treatment experiments, observed that application some cases reduced infection alleviated fitness impacts, while others, no significant noted. Notably, able persist within symbiont-infected over multiple culture transfers, indicating potential long-term interactions. Discussion findings highlight variability phage-symbiont environment underscore complex nature lays foundation for future studies exploring dynamics systems, suggesting mechanisms may shape differential outcomes presenting valuable avenues investigation.

Language: Английский

Phage cocktail administration to reduce Salmonella load in broilers DOI
Wattana Pelyuntha, Ruttayaporn Ngasaman, Mingkwan Yingkajorn

et al.

Research in Veterinary Science, Journal Year: 2024, Volume and Issue: 169, P. 105163 - 105163

Published: Jan. 25, 2024

Language: Английский

Citations

9
Fecal microbiota transplantation alters gut phage communities in a clinical trial for obesity DOI Creative Commons

Michele Zuppi,

Tommi Vatanen, Brooke C. Wilson

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: July 6, 2024

Abstract Background Fecal microbiota transplantation (FMT) is a therapeutic intervention used to treat diseases associated with the gut microbiome. In human microbiome, phages have been implicated in influencing health, successful engraftment of donor correlated FMT treatment efficacy. The impact that gastrointestinal exert on health has primarily connected their ability modulate bacterial communities gut. Nonetheless, how affects recipients’ phage populations, and turn, this influences environment, not yet fully understood. study, we investigated effects phageome composition participants within Gut Bugs Trial (GBT), double-blind, randomized, placebo-controlled trial efficacy treating obesity comorbidities adolescents. Stool samples collected from donors at time recipients four points (i.e., baseline 6 weeks, 12 26 weeks post-intervention), underwent shotgun metagenomic sequencing. Phage sequences were identified characterized silico examine evidence assess extent FMT-induced alterations composition. Results Donor engrafted stably following FMT, composing significant proportion for entire course study (33.8 ± 1.2% females 33.9 3.7% males). varied between was positively alpha diversity. caused shift toward donors’ increased diversity variability over time. Conclusions significantly altered recipients' and, overall, microbial populations. increase consistent population dynamics. This proposes play critical role modulating environment suggests novel approaches understanding altering recipient’s registration registered Australian New Zealand Clinical Trials Registry (ACTR N12615001351505). protocol: protocol available https://bmjopen.bmj.com/content/9/4/e026174 .

Language: Английский

Citations

8
The Emerging Role of the Gut Virome in Health and Inflammatory Bowel Disease: Challenges, Covariates and a Viral Imbalance DOI Creative Commons
Daan Jansen, Jelle Matthijnssens

Viruses, Journal Year: 2023, Volume and Issue: 15(1), P. 173 - 173

Published: Jan. 6, 2023

Virome research is a rapidly growing area in the microbiome field that increasingly associated with human diseases, such as inflammatory bowel disease (IBD). Although substantial progress has been made, major methodological challenges limit our understanding of virota. In this review, we describe must be considered to accurately report virome composition and current knowledge on health IBD. First, description shows strong biases related wetlab (e.g., VLP enrichment) bioinformatics approaches (viral identification classification). Second, IBD patients show consistent viral imbalances characterized by high relative abundance phages belonging Caudovirales low Microviridae. Simultaneously, sporadic contraction CrAss-like potential expansion lysogenic intestinal are observed. Finally, despite numerous studies have conducted diversity analysis, it difficult draw firm conclusions due biases. Overall, present many environmental factors influence virome, its consensus IBD, contributing hypothesis called “positive feedback loop” may play role pathophysiology

Language: Английский

Citations

22
Fecal virome transfer improves proliferation of commensal gut Akkermansia muciniphila and unexpectedly enhances the fertility rate in laboratory mice DOI Creative Commons
Torben Sølbeck Rasmussen, Caroline M. Junker Mentzel, Malene Refslund Danielsen

et al.

Gut Microbes, Journal Year: 2023, Volume and Issue: 15(1)

Published: May 7, 2023

Probiotics are intended to improve gastrointestinal health when consumed. However, the probiotics marketed today only colonize densely populated gut a limited extent. Bacteriophages comprise majority of viruses in human virome and there strong indications that they play important roles shaping microbiome. Here, we investigate use fecal transplantation (FVT, sterile filtrated feces) as mean alter microbiome composition lead way for persistent colonization two types probiotics: Lacticaseibacillus rhamnosus GG (LGG) representing well-established probiotic Akkermansia muciniphila (AKM) putative next-generation probiotic. Male female C57BL/6NTac mice were cohoused pairs from 4 weeks age received following treatment by oral gavage at week 5 6: AKM+FVT, LGG+FVT, sham (Pro-sham)+FVT, LGG+Saline, AKM+Saline, control (Pro-sham+Saline). The FVT donor material originated with high relative abundance A. muciniphila. All animals terminated 9 weeks. did not increase administered LGG or AKM recipient mice. Instead significantly (p < 0.05) increased naturally occurring compared control. This highlights potential propagating existing commensal “probiotics” have already permanently colonized gut. Being co-housed male female, fraction became pregnant. Unexpectedly, treated found higher fertility rate independent administration. These preliminary observations urge follow-up studies investigating interactions between fertility.

Language: Английский

Citations

18
Bacteriophages, gut bacteria, and microbial pathways interplay in cardiometabolic health DOI Creative Commons
Daniel Kirk, Ricardo Costeira, Alessia Visconti

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(2), P. 113728 - 113728

Published: Jan. 31, 2024

Cardiometabolic diseases are leading causes of mortality in Western countries. Well-established risk factors include host genetics, lifestyle, diet, and the gut microbiome. Moreover, bacterial communities their activities can be altered by bacteriophages (also known simply as phages), bacteria-infecting viruses, making these biological entities key regulators human cardiometabolic health. The manipulation populations phages enables possibility using treatment through phage therapy fecal viral transplants. First, however, a deeper understanding role phageome is required. In this review, we first introduce component microbiome discuss transplants relation to diseases. We then summarize current state research propose how might indirectly influence health bacteria metabolites.

Language: Английский

Citations

8
Gut commensal Enterocloster species host inoviruses that are secreted in vitro and in vivo DOI Creative Commons
Juan C. Burckhardt, Derrick Chong, Nicola Pett

et al.

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: March 30, 2023

Abstract Background Bacteriophages in the family Inoviridae , or inoviruses, are under-characterized phages previously implicated bacterial pathogenesis by contributing to biofilm formation, immune evasion, and toxin secretion. Unlike most bacteriophages, inoviruses do not lyse their host cells release new progeny virions; rather, they encode a secretion system that actively pumps them out of cell. To date, no inovirus associated with human gut microbiome has been isolated characterized. Results In this study, we utilized silico, vitro, vivo methods detect members microbiota. By screening representative genome library commensals, detected prophages Enterocloster spp. (formerly Clostridium spp.). We confirmed particles vitro cultures these organisms using imaging qPCR. assess how abiotic environment, physiology, may be linked, deployed tripartite assay progressively evaluated growth dynamics, presence changing osmotic environments. Counter other inovirus-producing bacteria, production was correlated formation Instead, strains had heterogeneous responses osmolality levels relevant physiology. Notably, increasing induced strain-dependent manner. gnotobiotic mouse model inoculated individual unperturbed conditions . Furthermore, consistent our observations, regulated changed environment due laxatives. Conclusion report on detection characterization novel from commensals genus. Together, results demonstrate gut-associated bacteria can secrete begin elucidate environmental niche filled commensal bacteria.

Language: Английский

Citations

12
Circulating T cell profiles associate with enterotype signatures underlying hematological malignancy relapses DOI Creative Commons
Nicolas Vallet, Maud Salmona, Jeanne Malet-Villemagne

et al.

Cell Host & Microbe, Journal Year: 2023, Volume and Issue: 31(8), P. 1386 - 1403.e6

Published: July 17, 2023

Language: Английский

Citations

12
Community Types of the Human Gut Virome are Associated with Endoscopic Outcome in Ulcerative Colitis DOI Creative Commons
Daan Jansen, Gwen Falony, Sara Vieira‐Silva

et al.

Journal of Crohn s and Colitis, Journal Year: 2023, Volume and Issue: 17(9), P. 1504 - 1513

Published: April 13, 2023

Inflammatory bowel disease [IBD] is a major debilitating disease. Recently, the gut microbiota has gained attention as an important factor involved in pathophysiology of IBD. As complement to established bacterial 'enterotypes' associated with IBD, we focused here on viruses. We investigated intestinal virome IBD patients undergoing biological therapy for presence configurations and uncover how those are therapeutic success.

Language: Английский

Citations

11
Exploring the gut DNA virome in fecal immunochemical test stool samples reveals associations with lifestyle in a large population-based study DOI Creative Commons
Paula Istvan, Einar Birkeland, Ekaterina Avershina

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 29, 2024

Stool samples for fecal immunochemical tests (FIT) are collected in large numbers worldwide as part of colorectal cancer screening programs. Employing FIT from 1034 CRCbiome participants, recruited a Norwegian study, we identify, annotate and characterize more than 18000 DNA viruses, using shotgun metagenome sequencing. Only six percent them assigned to known taxonomic family, with Microviridae being the most prevalent viral family. Linking individual profiles comprehensive lifestyle demographic data shows 17/25 variables be associated gut virome. Physical activity, smoking, dietary fiber consumption exhibit strong consistent associations both diversity relative abundance well enrichment auxiliary metabolic genes. We demonstrate suitability virome analysis, opening an opportunity large-scale studies this enigmatic microbiome. The diverse populations their connections uncovered herein paves way further exploration role health disease.

Language: Английский

Citations

4
Gut virome: New key players in the pathogenesis of inflammatory bowel disease DOI Open Access
‏Helal F. Hetta, Rehab Ahmed, Yasmin N. Ramadan

et al.

World Journal of Methodology, Journal Year: 2024, Volume and Issue: 15(2)

Published: Nov. 27, 2024

Inflammatory bowel disease (IBD) is a chronic inflammatory illness of the intestine. While mechanism underlying pathogenesis IBD not fully understood, it believed that complex combination host immunological response, environmental exposure, particularly gut microbiota, and genetic susceptibility represents major determinants. The virome group viruses found in great frequency gastrointestinal tract humans. varies greatly among individuals influenced by factors including lifestyle, diet, health conditions, geography, urbanization. majority research has focused on significance bacteria progression IBD, although viral populations represent an important component microbiome. We conducted this review to highlight communities their expected roles etiopathogenesis regarding published date.

Language: Английский

Citations

4